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<a href="http://doi.org/10.1371/journal.pone.0068528" target="_blank" rel="noreferrer noopener">http://doi.org/10.1371/journal.pone.0068528</a>
Pages
e68528–e68528
Issue
7
Volume
8
Dublin Core
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Title
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Bone marrow SSEA1+ cells support the myocardium in cardiac pressure overload.
Publisher
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PloS one
Date
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2013
1905-07
Subject
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Male; Animals; Mice; Mesenchymal Stem Cells/cytology/metabolism; Ventricular Remodeling; *Bone Marrow Transplantation; Bone Marrow Cells/cytology/*metabolism; Cell Tracking; Lewis X Antigen/*metabolism; Myocardium/cytology/*metabolism/pathology; Myocytes; Cardiac/cytology/metabolism
Creator
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Finan Amanda; Sopko Nikolai; Dong Feng; Turturice Ben; Kiedrowski Matthew; Penn Marc S
Description
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RATIONALE: Stage specific embryonic antigen 1+ (SSEA1+) cells have been described as the most primitive mesenchymal progenitor cell in the bone marrow. Cardiac injury mobilizes SSEA1+ cells into the peripheral blood but their in vivo function has not been characterized. OBJECTIVE: We generated animals with chimeric bone marrow to determine the fate and function of bone marrow SSEA1+ cells in response to acute cardiac pressure overload. METHODS AND RESULTS: Lethally irradiated mice were transplanted with normal bone marrow where the wild-type SSEA1+ cells were replaced with green fluorescent protein (GFP) SSEA1+ cells. Cardiac injury was induced by trans-aortic constriction (TAC). We identified significant GFP+ cell engraftment into the myocardium after TAC. Bone marrow GFP+ SSEA1 derived cells acquired markers of endothelial lineage, but did not express markers of c-kit+ cardiac progenitor cells. The function of bone marrow SSEA1+ cells after TAC was determined by transplanting lethally irradiated mice with bone marrow depleted of SSEA1+ cells (SSEA1-BM). The cardiac function of SSEA1-BM mice declined at a greater rate after TAC compared to their complete bone marrow transplant counterparts and was associated with decreased bone marrow cell engraftment and greater vessel rarefication in the myocardium. CONCLUSIONS: These results provide evidence for the recruitment of endogenous bone marrow SSEA1+ cells to the myocardium after TAC. We demonstrate that, in vivo, bone marrow SSEA1+ cells have the differentiation potential to acquire endothelial lineage markers. We also show that bone marrow SSEA1+ deficiency is associated with a reduced compensatory capacity to cardiac pressure overload, suggesting their importance in cardiac homeostasis. These data demonstrate that bone marrow SSEA1+ cells are critical for sustaining vascular density and cardiac repair to pressure overload.
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<a href="http://doi.org/10.1371/journal.pone.0068528" target="_blank" rel="noreferrer noopener">10.1371/journal.pone.0068528</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Bone Marrow Transplantation
2013
Animals
Bone Marrow Cells/cytology/*metabolism
Cardiac/cytology/metabolism
Cell Tracking
Department of Integrative Medical Sciences
Dong Feng
Finan Amanda
Kiedrowski Matthew
Lewis X Antigen/*metabolism
Male
Mesenchymal Stem Cells/cytology/metabolism
Mice
Myocardium/cytology/*metabolism/pathology
Myocytes
NEOMED College of Medicine
Penn Marc S
PloS one
Sopko Nikolai
Turturice Ben
Ventricular Remodeling