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<a href="http://doi.org/10.1016/j.neuroscience.2013.01.061" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.neuroscience.2013.01.061</a>
Pages
170–183
Volume
237
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Title
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Analysis of excitatory synapses in the guinea pig inferior colliculus: a study using electron microscopy and GABA immunocytochemistry.
Publisher
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Neuroscience
Date
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2013
2013-05
Subject
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*Inferior Colliculi/cytology/metabolism/ultrastructure; *Microscopy; Animals; Female; gamma-Aminobutyric Acid/*metabolism; Guinea Pigs; Immunoelectron; Male; NADPH Dehydrogenase/metabolism; Neurons/metabolism/*ultrastructure; Presynaptic Terminals/metabolism/ultrastructure; Synapses/*physiology/*ultrastructure
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Nakamoto K T; Mellott J G; Killius J; Storey-Workley M E; Sowick C S; Schofield B R
Description
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The inferior colliculus (IC) integrates ascending auditory input from the lower brainstem and descending input from the auditory cortex. Understanding how IC cells integrate these inputs requires identification of their synaptic arrangements. We describe excitatory synapses in the dorsal cortex, central nucleus, and lateral cortex of the IC (ICd, ICc and IClc) in guinea pigs. We used electron microscopy (EM) and post-embedding anti-GABA immunogold histochemistry on aldehyde-fixed tissue from pigmented adult guinea pigs. Excitatory synapses were identified by round vesicles, asymmetric synaptic junctions, and gamma-aminobutyric acid-immunonegative (GABA-negative) presynaptic boutons. Excitatory synapses constitute approximately 60% of the synapses in each IC subdivision. Three types can be distinguished by presynaptic profile area and number of mitochondrial profiles. Large excitatory (LE) boutons are more than 2 mum(2) in area and usually contain five or more mitochondrial profiles. Small excitatory (SE) boutons are usually less than 0.7 mum(2) in area and usually contain 0 or 1 mitochondria. Medium excitatory (ME) boutons are intermediate in size and usually contain 2 to 4 mitochondria. LE boutons are mostly confined to the ICc, while the other two types are present throughout the IC. Dendritic spines are the most common target of excitatory boutons in the IC dorsal cortex, whereas dendritic shafts are the most common target in other IC subdivisions. Finally, each bouton type terminates on both gamma-aminobutyric acid-immunopositive (GABA+) and GABA-negative (i.e., glutamatergic) targets, with terminations on GABA-negative profiles being much more frequent. The ultrastructural differences between the three types of boutons presumably reflect different origins and may indicate differences in postsynaptic effect. Despite such differences in origins, each of the bouton types contact both GABAergic and non-GABAergic IC cells, and could be expected to activate both excitatory and inhibitory IC circuits.
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<a href="http://doi.org/10.1016/j.neuroscience.2013.01.061" target="_blank" rel="noreferrer noopener">10.1016/j.neuroscience.2013.01.061</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Inferior Colliculi/cytology/metabolism/ultrastructure
*Microscopy
2013
Animals
Department of Anatomy & Neurobiology
Female
gamma-Aminobutyric Acid/*metabolism
Guinea Pigs
Immunoelectron
Killius J
Male
Mellott J G
NADPH Dehydrogenase/metabolism
Nakamoto K T
NEOMED College of Medicine
Neurons/metabolism/*ultrastructure
Neuroscience
Presynaptic Terminals/metabolism/ultrastructure
Schofield B R
Sowick C S
Storey-Workley M E
Synapses/*physiology/*ultrastructure