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40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajplung.00151.2007" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajplung.00151.2007</a>
Pages
L1069–1078
Issue
4
Volume
293
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Involvement of {alpha}ENaC and Nedd4-2 in the conversion from lung fluid secretion to fluid absorption at birth in the rat as assayed by RNA interference analysis.
Publisher
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American journal of physiology. Lung cellular and molecular physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007-10
Subject
The topic of the resource
*RNA Interference; Absorption; Animals; Body Fluids/*metabolism; Death; Endosomal Sorting Complexes Required for Transport; Epithelial Sodium Channels/genetics/*physiology; Extravascular Lung Water/metabolism; Gene Silencing; Lung/*metabolism; Nedd4 Ubiquitin Protein Ligases; Newborn/*metabolism; Rats; RNA; Small Interfering/metabolism; Sodium-Potassium-Exchanging ATPase/metabolism; Sprague-Dawley; Tissue Distribution; Ubiquitin-Protein Ligases/genetics/metabolism/*physiology
Creator
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Li Tianbo; Koshy Shyny; Folkesson Hans G
Description
An account of the resource
To explore interactions between the epithelial Na channel (ENaC) and neural precursor expressed, developmentally downregulated protein 4-2 (Nedd4-2) at the conversion of the rat lung from fluid secretion to absorption at birth, we used small-interfering RNA (siRNA) against alphaENaC and Nedd4-2. siRNA-generating plasmid DNA (pDNA) was administered via trans-thoracic intrapulmonary (ttip) injection 24 h before ENaC and Nedd4-2 expression, extravascular lung water, and mortality were measured. alphaENaC mRNA and protein were specifically reduced by approximately 65% after pSi-4 injection. Nedd4-2 mRNA and protein were reduced by approximately 60% after pSi-N1 injection. Interestingly, alphaENaC and betaENaC mRNA and protein expression were increased after Nedd4-2 silencing. Extravascular lung water was significantly increased after alphaENaC silencing and reduced after Nedd4-2 silencing. alphaENaC silencing resulted in a fourfold increase in newborn mortality, whereas silencing Nedd4-2 did not affect mortality. We also isolated distal lung epithelial (DLE) cells after in vivo alphaENaC or Nedd4-2 silencing and measured alphaENaC or Nedd4-2 expression in freshly isolated DLE cells. In these DLE cells, there were attenuated alphaENaC or Nedd4-2 mRNA and protein, thus demonstrating that alphaENaC and Nedd4-2 silencing occurred in alveolar epithelial cells after ttip injection. We also looked for pDNA by PCR to determine pDNA presence in the lungs and found strong evidence for pDNA presence in both lungs. Thus we provide evidence that ENaC and Nedd4-2 are involved in the transition from lung fluid secretion to fluid absorption near term and at birth.
Identifier
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<a href="http://doi.org/10.1152/ajplung.00151.2007" target="_blank" rel="noreferrer noopener">10.1152/ajplung.00151.2007</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*RNA Interference
2007
Absorption
American journal of physiology. Lung cellular and molecular physiology
Animals
Body Fluids/*metabolism
Death
Endosomal Sorting Complexes Required for Transport
Epithelial Sodium Channels/genetics/*physiology
Extravascular Lung Water/metabolism
Folkesson Hans G
Gene Silencing
Koshy Shyny
Li Tianbo
Lung/*metabolism
Nedd4 Ubiquitin Protein Ligases
Newborn/*metabolism
Rats
RNA
Small Interfering/metabolism
Sodium-Potassium-Exchanging ATPase/metabolism
Sprague-Dawley
Tissue Distribution
Ubiquitin-Protein Ligases/genetics/metabolism/*physiology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1186/1465-9921-9-55" target="_blank" rel="noreferrer noopener">http://doi.org/10.1186/1465-9921-9-55</a>
Pages
55–55
Volume
9
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
RNA interference for CFTR attenuates lung fluid absorption at birth in rats.
Publisher
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Respiratory research
Date
A point or period of time associated with an event in the lifecycle of the resource
2008
2008-07
Subject
The topic of the resource
*RNA Interference; Animals; Biological Transport/drug effects; Cells; Cultured; Cystic Fibrosis Transmembrane Conductance Regulator/*genetics/*metabolism; Epithelial Sodium Channels/genetics/metabolism; Female; Gene Expression Regulation/drug effects; Lung/cytology/drug effects/*metabolism; Messenger/metabolism; Newborn; Pregnancy; Rats; Respiratory Mucosa/cytology/drug effects/metabolism; RNA; Small Interfering/*pharmacology; Sprague-Dawley; Water/*metabolism
Creator
An entity primarily responsible for making the resource
Li Tianbo; Koshy Shyny; Folkesson Hans G
Description
An account of the resource
BACKGROUND: Small interfering RNA (siRNA) against alphaENaC (alpha-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs. METHODS: Twenty-four hours after trans-thoracic intrapulmonary (ttip) injection of siRNA-generating plasmid DNA (pSi-0, pSi-4, or pSi-C2), we measured CFTR and ENaC expression, extravascular lung water, and mortality. RESULTS: alphaENaC and CFTR mRNA and protein decreased by approximately 80% and approximately 85%, respectively, following alphaENaC and CFTR silencing. Extravascular lung water and mortality increased after alphaENaC and CFTR-silencing. In pSi-C2-transfected isolated DLE cells there were attenuated CFTR mRNA and protein. In pSi-4-transfected DLE cells alphaENaC mRNA and protein were both reduced. Interestingly, CFTR-silencing also reduced alphaENaC mRNA and protein. alphaENaC silencing, on the other hand, only slightly reduced CFTR mRNA and protein. CONCLUSION: Thus, ENaC and CFTR are both involved in the fluid secretion to absorption conversion around at birth.
Identifier
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<a href="http://doi.org/10.1186/1465-9921-9-55" target="_blank" rel="noreferrer noopener">10.1186/1465-9921-9-55</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*RNA Interference
2008
Animals
Biological Transport/drug effects
Cells
Cultured
Cystic Fibrosis Transmembrane Conductance Regulator/*genetics/*metabolism
Epithelial Sodium Channels/genetics/metabolism
Female
Folkesson Hans G
Gene Expression Regulation/drug effects
Koshy Shyny
Li Tianbo
Lung/cytology/drug effects/*metabolism
Messenger/metabolism
Newborn
Pregnancy
Rats
Respiratory Mucosa/cytology/drug effects/metabolism
Respiratory research
RNA
Small Interfering/*pharmacology
Sprague-Dawley
Water/*metabolism