1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g</a> Rights Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). Pages 241-249 Issue 4 Volume 17 Search for Full-text Locate full-text within NEOMED Library's e-journal collections <p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p> Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Specificity of cDNA-expressed human and rodent cytochrome P450s in the oxidative metabolism of the potent carcinogen 7,12-dimethylbenz a anthracene Publisher An entity responsible for making the resource available Molecular Carcinogenesis Date A point or period of time associated with an event in the lifecycle of the resource 1996 1996-12 Subject The topic of the resource 12-dimethylbenz(a)anthracene; 12-dimethylbenz(a)anthracene; 12-dimethylbenzanthracene metabolites; 7; binding; Biochemistry & Molecular Biology; cDNA expression; chromatography; cytochrome P450; high-performance liquid; human-tissues; hydrocarbons; liver; lung-cancer; metabolism of polycyclic aromatic; microsomes; mouse skin; Oncology; purification; rat; vaccinia virus Creator An entity primarily responsible for making the resource Shou M G; Korzekwa K R; Krausz K W; Buters J T M; Grogan J; Goldfarb I; Hardwick J P; Gonzalez F J; Gelboin H V Description An account of the resource 7,12-Dimethylbenz[a]anthracene (DMBA), a potent carcinogen, requires metabolic activation by cytochrome P450s (P450s) to electrophilic metabolites that result in DNA modification, mutagenicity, and carcinogenicity. In this study, we used eight human forms, four rodent forms, and one rabbit form of P450 expressed from recombinant vaccinia or baculovirus vectors to define their specificity for metabolizing DMBA. Of the eight human P450s, 1A1 was the most active (specific activity = 14.7 nmol/min/nmol of P450) in total metabolism of DMBA and showed approximately 6- to 33-fold more activity than other P450s. 2B6, 2C9, and 1A2 were also capable of metabolizing DMBA (2.0-2.5 nmol/min/nmol of P450), whereas 2C8, 2E1, 3A4, and 3A5 exhibited relatively low activities. Among animal P450s, mouse 1A1 exhibited activity similar to that of human 1A1 and had 5.0- to 37-fold more activity than other rodent and rabbit P450s. In regard to enzyme regioselectivity, most human and rodent P450s predominantly formed the 8,9-diol, but human 2B6 and rat 2B1 preferentially formed the 5,6-diol. In the production of monohydroxymethyl metabolites, all the enzymes yielded more 7-hydroxymethyl-12-methylbenz[a]anthracene (7HOM12MBA) than 12-hydroxymethyl-7-methylbenz[a]anthracene (7M12HOMBA), except for human 1A1, which presented the reverse selectivity. Human liver microsomes from 10 organ donors were shown to metabolize DM BA and in most circumstances generated the meta belie profile DM BA trans-8,9-d dihydrodiol > 7HOM12MBA greater than or equal to DMBA trans-5,6-dihydrodiol greater than or equal to 7,12-dihydroxymethylbenz[a]anthracene > 7M12HOMBA > DMBA trans-3,4-dihydrodiol. Thus, the combined activity of hepatic microsomal 2C9, 1A2, and 2B6 may contribute to the metabolic activation and the metabolism of DMBA in normal human liver. (C) 1996 Wiley-Liss, Inc. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g" target="_blank" rel="noreferrer noopener">10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g</a> Format The file format, physical medium, or dimensions of the resource Journal Article 12-dimethylbenz(a)anthracene 12-dimethylbenzanthracene metabolites 1996 7 Binding Biochemistry & Molecular Biology Buters J T M cDNA expression Chromatography cytochrome P450 Gelboin H V Goldfarb I Gonzalez F J Grogan J Hardwick J P high-performance liquid human-tissues Hydrocarbons Journal Article Korzekwa K R Krausz K W Liver lung-cancer metabolism of polycyclic aromatic Microsomes Molecular carcinogenesis mouse skin oncology purification rat Shou M G vaccinia virus