Expression Of Catalytically Active Cholesterol 7-alpha-hydroxylase In Escherichia-coli
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Li Y C; Chiang J Y L
Faseb Journal
1991
1991-03
Journal Article or Conference Abstract Publication
n/a
Prolactin Stimulates Dopamine Release From The Rat Corpus Striatum In The Absence Of Extracellular Calcium
amphetamine; behavior; binding-sites; brain; calcium; cerebrospinal-fluid; corpus striatum; dopamine; hypothalamus; in-vitro; Neurosciences & Neurology; prolactin; rat
Prolactin (PRL) increased basal dopamine (DA) release and attenuated amphetamine (AMPH)-stimulated DA release in vitro from rat corpus striatum in a concentration-dependent manner with 10(-5) M PRL being the most effective. The effects of PRL on DA release were enhanced in the absence of extracellular calcium. PRL at 10(-5) M did not alter the DA post-superfusion content of the striatal tissue. These results indicate that the stimulatory effect of PRL on basal DA release does not require extra-cellular calcium and the inhibitory effect on AMPH-stimulated DA release is not due to depletion of DA stores.
Laping N J; Dluzen D E; Ramirez V D
Neuroscience Letters
1991
1991-12
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0304-3940(91)90494-e" target="_blank" rel="noreferrer noopener">10.1016/0304-3940(91)90494-e</a>
Pulmonary-edema Produced By Pulmonary-hypertension After Cocaine Administration
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Lang S A; Maron M B
Faseb Journal
1991
1991-03
Journal Article or Conference Abstract Publication
n/a
Hyperpolarizing And Depolarizing Gaba-a Receptor-mediated Dendritic Inhibition In Area Ca1 Of The Rat Hippocampus
activity-dependent disinhibition; cortical-neurons; depression; electrophysiology; gamma-aminobutyric acid; lacunosum-moleculare interneurons; morphology; Neurosciences & Neurology; Physiology; pyramidal cells-invitro; responses; voltage-clamp
1. Gamma-aminobutyric acid(A) (GABA(A)) receptor-mediated inhibition of pyramidal neuron dendrites was studied in area CA1 of the rat hippocampal slice preparation with the use of intracellular and extracellular recording and one-dimensional current source-density (CSD) analysis. 2. Electrical stimulation of Schaffer collateral/commissural fibers evoked monosynaptic excitatory postsynaptic potentials (EPSPs) and population EPSPs, which were followed by biphasic inhibitory postsynaptic potentials (IPSPs). In the presence of the excitatory amino acid receptor antagonists 6,7-dinitroquinoxaline-2,3-dione (DNQX) and D,L-2-amino-5-phosphonovalerate (APV), stimulation in stratum radiatum evoked monosynaptic fast, GABA(A) and late, GABA(B) receptor or-mediated IPSPs and fast and late positive field potentials recorded in s. radiatum. 3. Fast monosynaptic IPSPs and fast positive field potentials evoked in the presence of DNQX and APV were reversibly abolished by the GABA(A) receptor antagonist bicuculline methiodide (BMI; 30-mu-M) and were not changed by the GABA(B) receptor antagonist P-[3-aminopropyl]-P-diethoxymethylphosphinic acid (CGP 35 348; 0.1-1.0 mM). CGP 35 348 (0.1 mM) reversibly blocked late monosynaptic IPSPs and late positive field potentials. These results suggest that fast field potentials are GABA(A) receptor-mediated population IPSPs (GABA(A), fast pIPSPs) and that late field potentials are GABA(B) receptor-mediated population IPSPs (GABA(B), late pIPSPs). 4. Fast pIPSPs were reversibly abolished when the extracellular Cl- concentration ([Cl-1]o) was reduced from 132 to 26 mM in parallel with a depolarizing shift in the reversal potential of fast IPSPs. Paired or repetitive stimulation in s. radiatum reversibly depressed fast pIPSPs and fast IPSPs. Paired-pulse depression of fast pIPSPs was reversibly antagonized by CGP 35 348 (0.40. 8 mM). 5. Laminar analysis of s. radiatum-evoked fast pIPSPs and one-dimensional CSD analysis revealed active current sources in s. radiatum and passive current sinks in s. oriens and s. lacunosum moleculare. S. radiatum sources were abolished by pressure application of BMI in s. radiatum but not in s. oriens. Stimulation in s. oriens, s. pyramidale, or s. lacunosum moleculare evoked GABA(A) current sources horizontal to the stimulation site. Changes in the dendritic location of inhibitory current with changes in stimulus location paralleled changes in the distribution of excitatory current. 6. In the presence of 4-aminopyridine (50-100-mu-M), DNQX and APV long-lasting depolarizing GABA(A) receptor-mediated responses (LLDs) occurred spontaneously or could be evoked. Current sinks associated with s. radiatum-evoked LLDs were located in the same dendritic area as sources associated with hyperpolarizing fast IPSPs. 7. These results suggest that activation of GABA(A) receptors located on pyramidal neuron apical and basal dendrites produces outward Cl-1 current and hyperpolarizing IPSPs. This suggests that depolarizing responses to dendritic GABA application and orthodromic activation in area CA1 do not result from inward chloride current.
Lambert N A; Borroni A M; Grover L M; Teyler T J
Journal of Neurophysiology
1991
1991-11
Journal Article or Conference Abstract Publication
n/a
Fluorescence Study Of A Mutant Cytochrome-b5 With A Single Tryptophan In The Membrane-binding Domain
b5; Biochemistry & Molecular Biology; energy; indole; mutagenesis; probes; residue; Spectroscopy; vesicles
Fluorescence studies of cytochrome b5 are complicated by the presence of three tryptophans, at positions 108, 109, and 112, in the membrane-binding domain. The cDNA for rabbit liver cytochrome b5, isolated from a lambda-gt11 library, was used to generate a mutated mRNA where the codons for tryptophans-108 and -112 were replaced by codons for leucine. The sequence was expressed in Escherichia coli and the mutant protein was isolated. This mutant protein had the expected absorption spectrum, and its amino acid composition was confirmed by amino acid analysis and by DNA sequencing of the construct. The fluorescence emission spectrum of the mutant is blue-shifted and is narrower than that of the native protein. The quantum yield of the mutant protein, per molecule, is only 60% of that of the native protein, and the enhancement when bound to lipid vesicles or detergent micelles is higher for the mutant. Fluorescence anisotropy measurements and quenching studies using brominated lipids suggest that the fluorescence of the native protein is due to tryptophans-109 and -108 while tryptophan-112 does not emit but undergoes nonradiative energy transfer to tryptophan-108. With this mutant, it was shown that incomplete energy transfer from tyrosines-126 and -129 to tryptophan-109 occurs when the membrane binding domain is inserted into lipid vesicles, which suggests that the membrane-binding domain does not exist in a tight hairpin loop.
Ladokhin A S; Wang L; Steggles A W; Holloway P W
Biochemistry
1991
1991-10
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1021/bi00106a018" target="_blank" rel="noreferrer noopener">10.1021/bi00106a018</a>
Neural Organization Of Spindles In 3 Hindlimb Muscles Of The Rat
Anatomy & Morphology; cat tenuissimus muscle; expression; fusimotor innervation; intrafusal fibers; lumbrical muscles; motor innervation; muscle; nuclear chain fibers; skeletofusimotor; soleus; ultrastructure
Kucera J; Walro J M; Reichler J
American Journal of Anatomy
1991
1991-01
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1002/aja.1001900107" target="_blank" rel="noreferrer noopener">10.1002/aja.1001900107</a>
Slow-tonic Mhc Expression In Paralyzed Hindlimbs Of Fetal Rats
Cell Biology; heavy-chain isoforms; innervation; intrafusal muscle-fibers; numbers; spindles
Whether nerve activity and active contraction of myotubes are essential for the assembly and initial differentiation of muscle spindles was investigated by paralyzing fetal rats with tetrodotoxin (TTX) from embryonic day 16 (E16) to E21, prior to and during the period when spindles typically form. TTX-treated soleus muscles were examined by light and electron microscopy for the presence of spindles and expression of myosin heavy chain (MHC) isoforms by the intrafusal fibers. Treatment with TTX did not inhibit the formation of a spindle capsule or the expression of a slow-tonic MHC isoform characteristic of intrafusal fibers, but did retard development of spindles. Spindles of TTX-treated E21 muscles usually consisted of one intrafusal fiber (bag2) only rather than two fibers (bag1 and bag2) typically present in untreated (control) E21 spindles. Intrafusal fibers of TTX-treated spindles also had only one sensory region supplied by multiple afferents, and were devoid of motor innervation. These features are characteristic of spindles in normal E18-E19 muscles. Thus, nerve and/or muscle activity is not essential for the assembly of muscle spindles, formation of a spindle capsule, and transformation of undifferentiated myotubes into the intrafusal fibers containing spindle-specific myosin isoforms. However, activity may promote the maturation of intrafusal bundles, as well as the maturation of afferent and efferent nerve supplies to intrafusal fibers.
Kucera J; Walro J M
Histochemistry
1991
1991
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1007/bf00266761" target="_blank" rel="noreferrer noopener">10.1007/bf00266761</a>
Development Of Muscle-spindles In Fetal Rats Treated With Tetrodotoxin
Neurosciences & Neurology; Physiology
Kucera J; Walro J M
Journal of Physiology-London
1991
1991-03
Journal Article or Conference Abstract Publication
n/a
Aggregation Of Myonuclei And The Spread Of Slow-tonic Myosin Immunoreactivity In Developing Muscle-spindles
Cell Biology; expression; fibers; heavy-chain isoforms; innervation; motor; rat; skeletal-muscle
The pattern of regional expression of a slowtonic myosin heavy chain (MHC) isoform was studied in developing rat soleus intrafusal muscle fibers. Binding of the slow-tonic antibody (ATO) began at the equator of prenatal intrafusal fibers where sensory nerve endings are located, and spread into the polar regions of nuclear bag2 and bag1 fibers but not nuclear chain fibers during ontogeny. The onset of the ATO reactivity coincided with the appearance of equatorial clusters of myonuclei (nuclear bag formations) in bag1 and bag2 fibers. Moreover, the intensity of the ATO reaction was strongest in the region of equatorial myonuclei and decreased with increasing distance from the equator of bag1 and bag2 fibers at all stages of prenatal and postnatal development. The polar expansion of ATO reactivity continued throughout the postnatal development of bag1 fibers, but ceased shortly after birth in bag2 fiber coincident with innervation by motor axons. Thus, afferents that innervate the equator might induce the slow-tonic MHC isoform in bag2 and bag1 fibers by regulating the myosin gene expression by equatorial myonuclei, and efferents or twitch contractile activity might inhibit the spread of the slow-tonic MHC isoform into the poles of bag2 but not bag1 fibers. Absence of ATO binding in chain fibers suggests that chain myotubes may not be as susceptible to the effect of afferents as are myotubes that develop into bag2 and bag1 fibers. The different patterns of slow-tonic MHC expression in the three types of intrafusal fiber may therefore result from the interaction of three elements: sensory neurons, motor neurons, and intrafusal myotubes.
Kucera J; Walro J M
Histochemistry
1991
1991
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1007/bf00315994" target="_blank" rel="noreferrer noopener">10.1007/bf00315994</a>
Alpha-2-macroglobulin - Binding-specificity For Nerve Growth-factor And Transforming Growth-factor-beta, And Its Possible Role In Growth-regulation
Biochemistry & Molecular Biology
Koo P H
Biological Chemistry Hoppe-Seyler
1991
1991-03
Journal Article or Conference Abstract Publication
n/a
Extrahepatic Cholestasis In The Rat Causes Up-regulation Of Hmg-coa-reductase (hmgr) And Cholesterol 7-alpha-hydroxylase (c7-alpha-h) At A Posttranscriptional Level
Gastroenterology & Hepatology
Jones M P; Vlahcevic Z R; Pandak W M; Heuman D M; Chiang J Y L; Hylemon P B
Hepatology
1991
1991-10
Journal Article or Conference Abstract Publication
n/a
Can Trait Anxiety, Grades, And Test-scores Measured Prior To Medical-school Matriculation Predict Clerkship Performance
academic-performance; Education & Educational Research; Health Care Sciences & Services; personality; stress; students
Jones B J
Academic Medicine
1991
1991-09
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1097/00001888-199109000-00029" target="_blank" rel="noreferrer noopener">10.1097/00001888-199109000-00029</a>
Higher Alpha-noradrenergic Receptors In Paraventricular Nucleus Of Obese Zucker Rats - Decline After Food-deprivation
adrenergic receptors; alpha-2-noradrenergic receptors; alpha-noradrenergic receptors; Behavioral Sciences; binding; brain-areas; circulating corticosterone; energy-expenditure; fa-fa; feeding behavior; feeding behavior; hypothalamic neuropeptide-y; hypothalamus; Neurosciences & Neurology; norepinephrine; obesity; paraventricular nucleus; Pharmacology & Pharmacy; zucker rats
Norepinephrine (NE), acting through alpha-2-noradrenergic receptors in the hypothalamic paraventricular nucleus (PVN), has been implicated in the control of feeding behavior and body weight gain. To determine whether this hypothalamic receptor system is disturbed in genetically obese rats, the binding of radioligands to alpha-2-noradrenergic, as well as to alpha-1-noradrenergic, receptors was examined in seven hypothalamic nuclei of obese Zucker rats relative to their lean littermates. Receptor binding procedures, using the alpha-2-noradrenergic agonist [H-3]p-aminoclonidine ([H-3]PAC) and the alpha-1-noradrenergic antagonist [H-3]prazosin, demonstrated that the obese rats, compared to the lean rats, had significantly greater alpha-2-noradrenergic and alpha-1-noradrenergic receptor binding, specifically in the PVN as opposed to other hypothalamic areas examined. Moreover, the obese rats, compared to the lean rats, exhibited greater responsiveness to the effects of food deprivation (48 h), which caused a significant decline in radioligand binding to both alpha-2 and alpha-1 receptors, specifically in the PVN. A decrease in alpha-2-receptor binding after deprivation in the obese rats was also seen in two basal hypothalamic areas, namely, the supraoptic nucleus and arcuate nucleus-median eminence. The possibility exists that these disturbances in hypothalamic alpha-receptors may be involved in the development and/or maintenance of the genetic obesity.
Jhanwaruniyal M; Awad I R; Gearhart G M; Finkelstein J A; Leibowitz S F
Pharmacology Biochemistry and Behavior
1991
1991-12
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0091-3057(91)90097-l" target="_blank" rel="noreferrer noopener">10.1016/0091-3057(91)90097-l</a>
Severe Nonimmune Hydrops Secondary To Parvovirus B-19 Infection - Spontaneous Reversal Inutero And Survival Of A Term Infant
b19 parvovirus; Obstetrics & Gynecology; pregnancy
We present a case of intrauterine infection with parvovirus B-19 and accompanying severe nonimmune hydrops at 26 weeks' gestation. The fetus showed progressive recovery on ultrasound. A term infant was delivered with hepatosplenomegaly as the only abnormality.
Humphrey W; Magoon M; Oshaughnessy R
Obstetrics and Gynecology
1991
1991-11
Journal Article or Conference Abstract Publication
n/a
Differential-diagnosis Of Chest Discomfort And General-approach To Myocardial-ischemia Decision-making
chest pain; clinical; differential diagnosis; Emergency Medicine
Howell J M; Hedges J R
American Journal of Emergency Medicine
1991
1991-11
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0735-6757(91)90118-4" target="_blank" rel="noreferrer noopener">10.1016/0735-6757(91)90118-4</a>
Alkalinity Of Nonindustrial Cleaning Products And The Likelihood Of Producing Significant Esophageal Burns
alkali; caustics; cleaning products; Emergency Medicine; ph
Howell J M
American Journal of Emergency Medicine
1991
1991-11
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0735-6757(91)90114-y" target="_blank" rel="noreferrer noopener">10.1016/0735-6757(91)90114-y</a>
Reduced Aortic Reactivity To Phenylephrine (pe) After Acute Exercise In Rabbits
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Howard M G; Dicarlo S E; Stallone J N
Faseb Journal
1991
1991-03
Journal Article or Conference Abstract Publication
n/a
Kipsaramon - A Lower Miocene Hominoid Site In The Tugen Hills, Baringo District, Kenya
Anthropology; ape; buluk; Evolutionary Biology; morphology; northern kenya
Hill A; Behrensmeyer K; Brown B; Deino A; Rose M; Saunders J; Ward S; Winkler A
Journal of Human Evolution
1991
1991-01
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0047-2484(91)90046-x" target="_blank" rel="noreferrer noopener">10.1016/0047-2484(91)90046-x</a>
Preventing Kinking Of The Peel-away Sheath During Insertion Of A Long-term Central Venous Catheter Using Percutaneous Subclavicular Venipuncture
Obstetrics & Gynecology; placement; Surgery
Heyd R L; Rosser J C
Surgery Gynecology & Obstetrics
1991
1991-02
Journal Article or Conference Abstract Publication
n/a
Recommendations For Monitoring Intracranial-pressure
Emergency Medicine
Heiselman D E
Annals of Emergency Medicine
1991
1991-01
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/s0196-0644(05)81141-2" target="_blank" rel="noreferrer noopener">10.1016/s0196-0644(05)81141-2</a>
Cyp4a Subfamily - Functional-analysis By Immunohistochemistry And Insitu Hybridization
acid omega-hydroxylation; Biochemistry & Molecular Biology; cells; clofibrate; cytochrome-p-450; genes; identification; kidney cortex microsomes; leukotriene-b4; sequence
Hardwick J P
Methods in Enzymology
1991
1991
Journal Article or Conference Abstract Publication
n/a
Response Of Obstructive Sleep-apnea To Fluoxetine And Protriptyline
follow-up; General & Internal Medicine; mechanism; nasal cpap; Respiratory System; stimulation; system; upper airway muscles; uvulopalatopharyngoplasty
Protriptyline is the pharmacologic agent most commonly used to treat obstructive sleep apnea (OSA); however, its anticholinergic side effects make it intolerable to many patients. Because serotonin may be a central respiratory stimulant and because the serotonin-uptake inhibitor, fluoxetine, is usually well tolerated, we wanted to try fluoxetine in the treatment of OSA. Therefore, we compared the effect of fluoxetine to that of protriptyline in 12 patients with OSA. Both drugs significantly decreased the proportion of REM sleep time and decreased the number of apneas or hypopneas in NREM sleep. The response to fluoxetine was equivalent to that of protriptyline; however, for the group as a whole, there was no significant improvement in the number of arterial oxygen desaturation events, the level of arterial oxygen desaturation, or the number of arousals with either agent. Although there was wide variability in the response to each medication, six of the 12 patients had good responses, including improvement in oxygenation, to either fluoxetine or protriptyline. Three patients could not complete the trial of protriptyline. We conclude that fluoxetine is beneficial to some, but not all, patients with OSA. Fluoxetine was better tolerated than protriptyline.
Hanzel D A; Proia N G; Hudgel D W
Chest
1991
1991-08
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1378/chest.100.2.416" target="_blank" rel="noreferrer noopener">10.1378/chest.100.2.416</a>
Effect Of Pathophysiological Plasma-concentrations Of Neuropeptide Y (npy) On Pulmonary Hemodynamics
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Maron M B; Lang S A; Maender K C; Pilati C F
Faseb Journal
1991
1991-03
Journal Article or Conference Abstract Publication
n/a
Purpura Fulminans And Adrenal Hemorrhage Due To Group-y Meningococcemia In An Elderly Woman
complement deficiencies; disease; General & Internal Medicine; neisseria-meningitidis; united-states
Marnejon T; Watanakunakorn C
Southern Medical Journal
1991
1991-04
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1097/00007611-199104000-00034" target="_blank" rel="noreferrer noopener">10.1097/00007611-199104000-00034</a>
Immunoreactivity Of Bufo-marinus Heart For Atrial-natriuretic-factor
amphibian; atrial natriuretic factor; body; factor anf; frog-heart; heart; immunohistochemistry; localization; peptide; ventricles; water regulation; Zoology
Gilloteaux J
Belgian Journal of Zoology
1991
1991-12
Journal Article or Conference Abstract Publication
n/a
Long-term Health Impacts Of Forced Early Retirement Among Steelworkers
employment; General & Internal Medicine; personnel turnover health
Gillanders W R; Buss T F; Wingard E; Gemmel D
Journal of Family Practice
1991
1991-04
Journal Article or Conference Abstract Publication
n/a
Managing Jehovahs-witnesses - Medical, Legal, And Ethical Challenges
Emergency Medicine
Fontanarosa P B; Giorgio G T
Annals of Emergency Medicine
1991
1991-10
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/s0196-0644(05)81396-4" target="_blank" rel="noreferrer noopener">10.1016/s0196-0644(05)81396-4</a>
Treatment Of Bacterial Skin And Soft-tissue Infections
bypass-surgery; diabetic foot infections; disease; moxalactam; Obstetrics & Gynecology; pharmacokinetics; plus clavulanic acid; recurrent cellulitis; resistance; staphylococci; Surgery; timentin
File T M; Tan J S
Surgery Gynecology & Obstetrics
1991
1991
Journal Article or Conference Abstract Publication
n/a
Ticarcillin-clavulanate Therapy For Bacterial Skin And Soft-tissue Infections
acid; Immunology; Microbiology; moxalactam; penetration; pharmacokinetics; potassium; safety; timentin
File T M; Tan J S
Reviews of Infectious Diseases
1991
1991-07
Journal Article or Conference Abstract Publication
n/a
Full-thickness Rotator-cuff Tear - An Analysis Of Results
Orthopedics; ruptures; Surgery
Essman J A; Bell R H; Askew M
Clinical Orthopaedics and Related Research
1991
1991-04
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1097/00003086-199104000-00018" target="_blank" rel="noreferrer noopener">10.1097/00003086-199104000-00018</a>
The Effect Of Cocaine On Myocardial-contractility
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Espinal A; Pukys T; Pilati C F
Faseb Journal
1991
1991-03
Journal Article or Conference Abstract Publication
n/a
Professional Oaths - Pharmacy Can Lead The Way
Pharmacology & Pharmacy
Erlen J A; Erlen J; Dickstein E
Journal of Clinical Pharmacy and Therapeutics
1991
1991-10
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1111/j.1365-2710.1991.tb00319.x" target="_blank" rel="noreferrer noopener">10.1111/j.1365-2710.1991.tb00319.x</a>
The Effect Of Long-term Treatment With Deprenyl On Basal And L-dopa Evoked Dopamine Release Invitro From The Corpus Striatum Of Aged Rats
(-)deprenyl; aged; amphetamine; brain; corpus striatum; (-)deprenyl; dopac; dopamine; l-dopa; life; Neurosciences & Neurology; parkinsons-disease
Dluzen D E; McDermott J L
Journal of Neural Transmission-General Section
1991
1991
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1007/bf01244706" target="_blank" rel="noreferrer noopener">10.1007/bf01244706</a>
Cardiac Afferents (ca) Inhibit Active Vasodilation (av) In The Rabbit Ear
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
DiCarlo S E; Collins H L
Faseb Journal
1991
1991-03
Journal Article or Conference Abstract Publication
n/a
The Evolution Of Surgical Endoscopic Training - Meeting The American Board Of Surgery Requirements
fiberoptic endoscopy; program; residents; Surgery; university
Cullado M J; Porter J A; Slezak F A
American Surgeon
1991
1991-04
Journal Article or Conference Abstract Publication
n/a
The Local Monoaminergic Dependency Of Spinal Ketamine
(intrathecal); 5-ht (5-hydroxytryptamine; analgesia; antinociception; cord; inhibition; ketamine; neurons; norepinephrine; opiate; opioid receptors; optical isomers; pharmacology; Pharmacology & Pharmacy; rat-brain; receptor; serotonin; tail-flick test
Crisp T; Perrotti J M; Smith D L; Stafinsky J L; Smith D J
European Journal of Pharmacology
1991
1991-03
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0014-2999(91)90101-u" target="_blank" rel="noreferrer noopener">10.1016/0014-2999(91)90101-u</a>
VAGINOSONOGRAPHIC MEASUREMENT OF ENDOMETRIAL THICKNESS IN THE EVALUATION OF AMENORRHEA
Reproductive Biology; Obstetrics & Gynecology; ultrasonic assessment
The progestin-induced withdrawal bleeding test has often been used in patients with amenorrhea to assess endogenous estrogen (E) production. The endometrial thickness measured by vaginal ultrasonography with a 5 MHz transducer is also dependent on E stimulation of the endometrium. In this study, 70 consecutive patients were evaluated with both the progestin-induced withdrawal bleeding test and a measurement of the endometrial thickness by vaginosonography. An endometrial thickness of 1.5 mm or less was selected to predict absence of bleeding after a progesterone (P) challenge test. This resulted in a sensitivity of 94% with a 95% confidence interval (CI) of 0.70 to 1.00 and a specificity of 93% with a 95% CI of 0.82 and 0.98. The positive and negative predictive values were 79% and 98%, respectively. This study shows that the endometrial thickness measured by vaginosonography can predict the results of the P challenge test in patients with amenorrhea.
Morcos R N; Leonard M D; Smith M; Bourguet C C; Makii M; Khawli O
Fertility and Sterility
1991
1991-03
Journal Article or Conference Abstract Publication
n/a
EVALUATION OF THE INTERACTIONS OF SEROTONERGIC AND ADRENERGIC-DRUGS WITH MU, DELTA, AND KAPPA OPIOID BINDING-SITES
receptor; Neurosciences & Neurology; involvement; rat-brain; morphine; dorsal horn; adrenergic; cord; mu opioid; nociceptive; periaqueductal gray; phentolamine; raphe magnus stimulation; reflexes; serotonergic; spinal antinociceptive action; spiroxatrine; delta opioid; kappa opioid
Several serotonergic and adrenergic agents were tested for an ability to interact with mu, delta, and kappa opioid binding sites. Spiroxatrine interacted nearly equipotently with all three opioid subtypes, yielding K(i) values near 110 nM. A number of other serotonergic and adrenergic agents interacted with affinities in the 1-50-mu-M range. Most of the other compounds tested in this study were found to compete for opioid binding to some degree, though not achieving a 50% inhibition of binding at concentrations up to 100-mu-M. If this interaction between monoaminergic agents and opioid receptors is found to have functional significance, it must be considered in the interpretation of results from studies using these agents to evaluate the contribution of monoaminergic systems to opioid-mediated events.
Monroe P J; Perschke S E; Crisp T; Smith D J
Neuroscience Letters
1991
1991-12
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/0304-3940(91)90576-f" target="_blank" rel="noreferrer noopener">10.1016/0304-3940(91)90576-f</a>
ULTRASTRUCTURAL CHARACTERISTICS OF A CELL-LINE DERIVED FROM A MELANOTIC NEUROECTODERMAL TUMOR OF INFANCY
Pathology; cell line; Dentistry; Oral Surgery & Medicine; freeze-fracture; melanotic neuroectodermal tumor of infancy; transmission electron microscopy
Thin section and freeze-fracture transmission electron microscopy were used to examine and identify the cytoplasmic and membrane structures in a cell line derived from a melanotic neuroectodermal tumor of infancy (MNTI). The cultured cells had a uniform appearance after 70 population doublings characterized by long dendritic processes and evidence of melanin production. The cytoplasm contained numerous melanosomes in various stages of development, vesiculated rough endoplasmic reticulum, microfilaments and uncoated as well as coated vesicles. The membrane specializations included caveoli, coated pits, gap junctions, microfilaments, desmosome-like structures and lamellipodia. The ultrastructural appearance of the cultured MNTI cells was similar to features previously seen in electron micrographs of MNTI tumor specimens. However, correlated freeze-fracture and thin section micrographs permitted further identification of structures previously described. The MNTI cell line represents one of the cell types of the tumor and provides an opportunity for further study of the pathogenesis of this rare tumor.
Claman L J; Stetson D; Steinberg B; Shuler C F
Journal of Oral Pathology & Medicine
1991
1991-05
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1111/j.1600-0714.1991.tb00428.x" target="_blank" rel="noreferrer noopener">10.1111/j.1600-0714.1991.tb00428.x</a>
REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ASSAY OF CHOLESTEROL 7-ALPHA-HYDROXYLASE
Biochemistry & Molecular Biology; cloning; enzyme; purification; rat-liver microsomes; cholesterol 7-alpha-hydroxylase
Chiang J Y L
Methods in Enzymology
1991
1991
Journal Article or Conference Abstract Publication
n/a