Characterization Of Regional Cerebral Blood Flow And Expression Of Angiogenic Growth Factors In The Frontal Cortex Of Juvenile Male Shrsp And Shr
abnormalities; AD/HD; angiogemic factor; animal-model; attention-deficit/hyperactivity; brain; children; deficit-hyperactivity-disorder; disorder; frontal cortex; Neurosciences & Neurology; nitric-oxide; NOS isoform; regional cerebral blood flow; spontaneously hypertensive-rats; stroke-prone; vegf
Attention-deficit/hyperactivity disorder (AD/HD) is a common pediatric behavioral disorder associated with male preponderance and reduction of regional cerebral blood flow (rCBF). However, lack of an appropriate animal model exhibiting appropriate AD/HD symptoms stands in the way of studying mechanism(s) underlying reduced rCBF and male preponderance. Our group has been investigating the suitability of juvenile male stroke-prone spontaneously hypertensive rats (SHRSP), a substrain of the commonly used AD/HD animal model SHR, as a model for AD/HD because, unlike SHR, SHRSP displays cognitive impairment and male preponderance. Our more recent studies revealed alterations in the synthesis of sex steroid hormones and angiogenic factors in the frontal cortex of male SHRSP compared to the genetic control WKY. Based on these observations, the present study utilizes laser-Doppler flowmetry, histochemistry, enzyme immunoassay, immunoblotting, and real-time PCR to characterize and compare the patterns of regional cerebral blood flow and synthesis of angiogenic molecules [basic fibroblast growth factor; nitric oxide synthase isoforms (endothelial, neuronal and inducible); vascular endothelial growth factor (VEGF) and its signaling molecules VEGF receptors, phosphorylated Akt, endothelial nitric oxide synthase eNOS] between male SHRSP and SHR. Overall, consistent with our previous data showing alteration in VEGF/Akt/NO signaling, there was a marked reduction in the profile of rCBF (35%) and angiogenic factors of SHRSP, compared to age-matched genetic control Wistar-Kyoto rats (WKY) and SHR. We conclude that, unlike SHR, the profiles of rCBF and angiogenic factors in SHRSP are altered in juvenile male. Thus, SHRSP appears to be a more suitable animal model for studying changes in rCBF in AD/HD. (C) 2004 Elsevier B.V. All rights reserved.
Jesmin S; Togashi H; Mowa C N; Ueno K; Yamaguchi T; Shibayama A; Miyauchi T; Sakuma I; Yoshioka M
Brain Research
2004
2004-12
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1016/j.brainres.2004.10.004" target="_blank" rel="noreferrer noopener">10.1016/j.brainres.2004.10.004</a>
The Trouble With Flippers: A Report On The Prevalence Of Digital Anomalies In Cetacea
abnormalities; bottle-nosed-dolphin; evolution; flipper; forelimb; hyperphalangy; limb; limb malformations; manus; mechanobiology; patterns; polydactyly; polyphalangy; tursiops-truncatus; vertebrate; Zoology
Cooper L N; Dawson S D
Zoological Journal of the Linnean Society
2009
2009-03
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1111/j.1096-3642.2008.00454.x" target="_blank" rel="noreferrer noopener">10.1111/j.1096-3642.2008.00454.x</a>
Madelung Deformity In Skeletally Immature Patients: Morphologic Assessment Using Radiography, Ct, And Mri
abnormalities; anomalies; bones; computed tomography; congenital; Deformity; Madelung; magnetic resonance imaging; Nuclear Medicine & Medical Imaging; Radiology
Cook P A; Yu J S; Wiand W; Lubbers L; Coleman C R; Cook A J; Kean J R
Journal of Computer Assisted Tomography
1996
1996-07
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1097/00004728-199607000-00001" target="_blank" rel="noreferrer noopener">10.1097/00004728-199607000-00001</a>
The association of sacroiliac joint bridging with other enthesopathies in the human body
Orthopedics; Neurosciences & Neurology; disease; prevalence; fusion; ankylosing-spondylitis; arthritis; spondyloarthropathy; abnormalities; criteria; erosive; idiopathic skeletal hyperostosis; ankylosing-spondylitis; sacroiliac joint; diffuse idiopathic skeletal hyperostosis; dish; ankylosing; entheseal; forestier; reaction; spinal diseases
Study Design. A descriptive study of the association between sacroiliac joint (extra-articular) bridging and other enthesopathies. Objectives. To examine the relationship between sacroiliac joint bridging with other entheseal reaction sites in the skeleton, and its prognostic value in spinal diseases. Summary of Background Data. Sacroiliac joint bridging is considered a hallmark of spinal diseases ( e. g., ankylosing spondylitis). Nevertheless, its association with other enthesopathies has never been quantified and analyzed. Methods. A total of 289 human male skeletons with sacroiliac joint bridging and 127 without ( of similar demographic structure) were evaluated for the presence of entheseal ossification, cartilaginous calcification, and other axial skeleton joint fusion ( a total of 18 anatomic sites). The presence of diffuse idiopathic skeletal hyperostosis and spondyloarthropathy was also recorded. Results. Sacroiliac joint bridging was strongly associated with entheseal reactions in other parts of the body. Of the sacroiliac joint bridging group, 24.91% had diffuse idiopathic skeletal hyperostosis, and 8.05% had spondyloarthropathy. Conclusions. The presence of sacroiliac joint bridging indicates an intensive general entheseal process in the skeleton.
Dar G; Peleg S; Masharawi Y; Steinberg N; Rothschild B M; Hershkovitz I
Spine
2007
2007-05
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1097/01.brs.0000261568.88404.18" target="_blank" rel="noreferrer noopener">10.1097/01.brs.0000261568.88404.18</a>
RAPID REGRESSION OF CORONARY DILATATION IN KAWASAKI-DISEASE WITH INTRAVENOUS GAMMA-GLOBULIN
abnormalities; arterial aneurysm; Cardiovascular System & Cardiology; children; dimensional echocardiography; follow-up; infants; lymph-node syndrome
Saalouke M G; Venglarcik J S; Baker D R; Saalouke Z I; Bashour T T
American Heart Journal
1991
1991-03
Journal Article
<a href="http://doi.org/10.1016/0002-8703(91)90207-x" target="_blank" rel="noreferrer noopener">10.1016/0002-8703(91)90207-x</a>
Knockdown of amyloid precursor protein normalizes cholinergic function in a cell line derived from the cerebral cortex of a trisomy 16 mouse: An animal model of Down syndrome
16 mice; abnormalities; acetylcholine; acetylcholine-release; alzheimers-disease; amyloid; antisense; beta-protein; calcium; cell line; Down syndrome; Neurosciences & Neurology; neurotoxicity; peptide; rat hippocampal slices; root ganglion neurons
We have generated immortal neuronal cell lines from normal and trisomy 16 (Ts16) mice, a model for Down syndrome (DS). Ts16 lines overexpress DS-related genes (App, amyloid precursor protein; Sod1, Cu/Zn superoxide dismutase) and show altered cholinergic function (reduced choline uptake, ChAT expression and fractional choline release after stimulation). As previous evidence has related amyloid to cholinergic dysfunction, we reduced APP expression using specific mRNA antisense sequences in our neuronal cell line named CTb, derived from Ts16 cerebral cortex, compared to a cell line derived from a normal animal, named CNh. After transfection, Western blot studies showed APP expression knockdown in CTb cells of 36% (24 hr), 40.4% (48 hr), and 50.2% (72 hr) compared to CNh. Under these reduced APP levels, we studied 3 H-choline uptake in CTb and CNh cells. CTb, as reported previously, expressed reduced choline uptake compared to CNh cells (75%, 90%, and 69% reduction at 1, 2, and 5 min incubation, respectively). At 72 hr of APP knockdown, choline uptake levels were essentially similar in both cell types. Further, fractional release of H-3-choline in response to glutamate, nicotine, and depolarization with KCI showed a progressive increase after APP knockdown, reaching values similar to those of CNh after 72 hr of transfection. The results suggest that APP overexpression in CTb cells contributes to impaired cholinergic function, and that gene knockdown in CTb cells is a relevant tool to study DS-related dysfunction. (c) 2006 Wiley-Liss, Inc.
Opazo P; Saud K; de Saint Pierre M; Cardenas A M; Allen D D; Segura-Aguilar J; Caviedes R; Caviedes P
Journal of Neuroscience Research
2006
2006-11
Journal Article
<a href="http://doi.org/10.1002/jnr.21035" target="_blank" rel="noreferrer noopener">10.1002/jnr.21035</a>
Extra-amniotic pregnancy with fetal gastroschisis and clubfoot.
Female; Humans; Ultrasonography; Pregnancy; Amnion/diagnostic imaging; Clubfoot/*diagnostic imaging/embryology; Gastroschisis/*diagnostic imaging/embryology; Abnormalities; Ectopic/*diagnostic imaging; Multiple/*diagnostic imaging; Prenatal/*methods
Baca Diana; Thomas Ronald L; Celebrezze Jennifer U; Golde Steven H
Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine
2009
2009-01
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.7863/jum.2009.28.1.77" target="_blank" rel="noreferrer noopener">10.7863/jum.2009.28.1.77</a>
Management of Bilateral Undescended Bilobed Testes and Review of the Literature.
*Abnormalities; Abnormalities; Cryptorchidism – Complications; Cryptorchidism – Surgery; Cryptorchidism/*complications/surgery; Humans; Infant; Male; Multiple – Surgery; Multiple/surgery; Testis – Abnormalities; Testis – Surgery; Testis/*abnormalities/surgery
Polyorchidism is a rare anomaly of testicular development. Particularly, a bilobed testis is an extremely rare congenital malformation, which is thought to be a variant expression of polyorchidism. Only 5 cases of bilobed testis have been reported in the literature to date. This report is of bilateral, undescended, bilobed testes in a 15-month-old boy who has multiple other malformations of possible genetic etiology.
Cohen Tal; Agard Hannah; Parekh Neel; Clark Curtis
Urology
2017
2017-12
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/j.urology.2017.08.026" target="_blank" rel="noreferrer noopener">10.1016/j.urology.2017.08.026</a>
Gastroschisis: an 18-year review.
Abdominal Muscles/*abnormalities/surgery; Abnormalities; Cesarean Section; Congenital Abnormalities/surgery; Female; Humans; Infant; Male; Methods; Multiple; Newborn; Postoperative Complications; Retrospective Studies
From 1972 to 1990, 69 cases of gastroschisis were treated at Akron Children's Hospital Medical Center. Eighty-one percent of these patients underwent primary closure of their abdominal wall defect. Thirteen of 69 patients (19%) required Silastic silos with final closure in an average of 7.8 days. There was no sex predilection, the average birth weight was 2,473 g, and the mean gestational age was 36.3 weeks. Twenty-six percent had associated anomalies, the majority were intestinal atresia, volvulus, and/or undescended testicles. Seventy-seven percent of the infants were delivered vaginally. Fourteen children were delivered via cesarean section. Seven cesarean sections were done solely for prenatal ultrasonic identification of an abdominal wall defect. There was no improvement in hospital stay, complications, days until enteral feeds were tolerated, days intubated, or number of surgical procedures in this group. In 14 patients, mesh sheeting (Marlex, Silastic) was used in the final closure. Sixty-four percent of these incurred wound breakdown necessitating removal of the mesh. This compares with a 3.2% wound breakdown in the nonmesh group. The average hospital stay was 43.9 days and the average time to enteral feeds 20.2 days. Sixty-four percent of the patients required postoperative intubation for an average of 5.5 days. The overall mortality rate was 4.3%. The present data do not support gastroschisis alone as an indication for cesarean section. The data indicate that mesh be avoided in the final closure if possible and support a favorable prognosis for most babies.
Novotny D A; Klein R L; Boeckman C R
Journal of pediatric surgery
1993
1993-05
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0022-3468(93)90022-d" target="_blank" rel="noreferrer noopener">10.1016/0022-3468(93)90022-d</a>