1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.neuroscience.2009.02.036" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.neuroscience.2009.02.036</a>
Pages
103–114
Issue
1
Volume
160
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Sources of cholinergic input to the inferior colliculus.
Publisher
An entity responsible for making the resource available
Neuroscience
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-04
Subject
The topic of the resource
Acetylcholine/*metabolism; Animals; Choline O-Acetyltransferase/metabolism; Female; Guinea Pigs; Immunohistochemistry; Inferior Colliculi/*anatomy & histology; Male; Neural Pathways/anatomy & histology/metabolism; Pedunculopontine Tegmental Nucleus/anatomy & histology/metabolism; Photomicrography; Tegmentum Mesencephali/*anatomy & histology/*metabolism
Creator
An entity primarily responsible for making the resource
Motts S D; Schofield B R
Description
An account of the resource
We combined retrograde tracing with immunohistochemistry for choline acetyltransferase to identify the source of cholinergic input to the inferior colliculus (IC) in guinea pigs. Injection of a retrograde tracer into one IC labeled cells in many brainstem nuclei. Retrogradely-labeled cells that were also immunoreactive for choline acetyltransferase were identified in two nuclei in the midbrain tegmentum: the pedunculopontine tegmental nucleus (PPT) and the laterodorsal tegmental nucleus (LDT). More PPT and LDT cells project ipsilaterally than contralaterally to the IC and, on both sides, there are more projecting cells in the PPT than in the LDT. Double-labeled cells were not found in any other brainstem nucleus. A common feature of cholinergic cells in PPT and LDT is collateral projections to multiple targets. We placed different retrograde tracers into each IC to identify cells in PPT and LDT that project to both ICs. In both PPT and LDT, a substantial proportion (up to 57%) of the immunoreactive cells that contained tracer from the contralateral IC also contained tracer from the ipsilateral IC. We conclude that acetylcholine in the IC originates from the midbrain tegmental cholinergic nuclei: PPT and LDT. These nuclei are known to participate in arousal, the sleep/wake cycle and prepulse inhibition of acoustic startle. It is likely that the cholinergic input to the IC is directly associated with these functions.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.neuroscience.2009.02.036" target="_blank" rel="noreferrer noopener">10.1016/j.neuroscience.2009.02.036</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
Acetylcholine/*metabolism
Animals
Choline O-Acetyltransferase/metabolism
Department of Anatomy & Neurobiology
Female
Guinea Pigs
Immunohistochemistry
Inferior Colliculi/*anatomy & histology
Male
Motts S D
NEOMED College of Medicine
Neural Pathways/anatomy & histology/metabolism
Neuroscience
Pedunculopontine Tegmental Nucleus/anatomy & histology/metabolism
Photomicrography
Schofield B R
Tegmentum Mesencephali/*anatomy & histology/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.neuroscience.2009.12.008" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.neuroscience.2009.12.008</a>
Pages
231–240
Issue
1
Volume
166
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Projections from auditory cortex to midbrain cholinergic neurons that project to the inferior colliculus.
Publisher
An entity responsible for making the resource available
Neuroscience
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-03
Subject
The topic of the resource
Acetylcholine/*metabolism; Amidines; Animals; Auditory Cortex/*cytology/metabolism; Auditory Perception/physiology; Brain Mapping; Choline O-Acetyltransferase/metabolism; Cholinergic Fibers/metabolism/ultrastructure; Dextrans; Female; Functional Laterality/physiology; Guinea Pigs; Immunohistochemistry; Inferior Colliculi/*cytology/metabolism; Male; Mesencephalon/*cytology/metabolism; Neural Pathways/cytology/metabolism; Neuroanatomical Tract-Tracing Techniques; Neuronal Tract-Tracers; Neurons/*cytology/metabolism; Pedunculopontine Tegmental Nucleus/*cytology/metabolism; Rhodamines; Synaptic Transmission/physiology
Creator
An entity primarily responsible for making the resource
Schofield B R
Description
An account of the resource
We have shown that auditory cortex projects to cholinergic cells in the pedunculopontine tegmental nucleus (PPT) and laterodorsal tegmental nucleus (LDT). PPT and LDT are the sources of cholinergic projections to the inferior colliculus, but it is not known if the cortical inputs contact the cholinergic cells that project to the inferior colliculus. We injected FluoroRuby into auditory cortex in pigmented guinea pigs to label cortical projections to PPT and LDT. In the same animals, we injected Fast Blue into the left or right inferior colliculus to label PPT and LDT cells that project to the inferior colliculus. We processed the brain to identify cholinergic cells with an antibody to choline acetyltransferase, which was visualized with a green fluorescent marker distinguishable from both FluoroRuby and Fast Blue. We then examined the PPT and LDT to determine whether boutons of FluoroRuby-labeled cortical axons were in close contact with cells that were double-labeled with the retrograde tracer and the immunolabel. Apparent contacts were observed ipsilateral and, less often, contralateral to the injected cortex. On both sides, the contacts were more numerous in PPT than in LDT. The results indicate that auditory cortex projects directly to brainstem cholinergic cells that innervate the ipsilateral or contralateral inferior colliculus. This suggests that cortical projections could elicit cholinergic effects on both sides of the auditory midbrain.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.neuroscience.2009.12.008" target="_blank" rel="noreferrer noopener">10.1016/j.neuroscience.2009.12.008</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2010
Acetylcholine/*metabolism
Amidines
Animals
Auditory Cortex/*cytology/metabolism
Auditory Perception/physiology
Brain Mapping
Choline O-Acetyltransferase/metabolism
Cholinergic Fibers/metabolism/ultrastructure
Department of Anatomy & Neurobiology
Dextrans
Female
Functional Laterality/physiology
Guinea Pigs
Immunohistochemistry
Inferior Colliculi/*cytology/metabolism
Male
Mesencephalon/*cytology/metabolism
NEOMED College of Medicine
Neural Pathways/cytology/metabolism
Neuroanatomical Tract-Tracing Techniques
Neuronal Tract-Tracers
Neurons/*cytology/metabolism
Neuroscience
Pedunculopontine Tegmental Nucleus/*cytology/metabolism
Rhodamines
Schofield B R
Synaptic Transmission/physiology