Ultrastructural immunolocalization of the atrial natriuretic factor pathways in fetal, neonatal, and adult Syrian hamsters: from the atrial cardiomyocytes to the circulation via the endocardium, atrial capillaries and epicardium.
Animals; Cricetinae; Aging/*metabolism; Mesocricetus; Microscopy; Atrial Natriuretic Factor/*metabolism; Capillaries/cytology/metabolism/ultrastructure; Endocardium/cytology/metabolism/ultrastructure; Myocardium/metabolism/*ultrastructure; Endothelium; Immunoelectron; Newborn/*metabolism; Vascular/cytology/metabolism/ultrastructure
Atrial natriuretic factor (ANF)-immunoreactivity was identified with peroxidase-antiperoxidase staining and immunoelectron microscopic gold labeling techniques in atrial tissues of fetal, newborn and adult Syrian hamsters.
Gilloteaux J; Jennes L; Menu R; Vanderhaeghen J J
Journal of submicroscopic cytology and pathology
1991
1991-01
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Alterations in neurotransmitter amino acid content in the aging rat striatum are subregion dependent.
Male; Animals; Rats; Aging/*metabolism; Amino Acids/*analysis; Neurotransmitter Agents/*analysis; Corpus Striatum/*analysis; Inbred F344
The topographical distribution of putative neurotransmitter amino acids in both
Donzanti B A; Ung A K
Neurobiology of aging
1990
1990-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0197-4580(90)90050-a" target="_blank" rel="noreferrer noopener">10.1016/0197-4580(90)90050-a</a>
The effects of aging on spinal neurochemistry in the rat.
Aging/*metabolism; Animals; Chromatography; Dopamine/metabolism; Electrochemistry/methods; High Pressure Liquid; Inbred F344; Male; Norepinephrine/metabolism; Rats; Serotonin/metabolism; Spinal Cord/*metabolism
The purpose of this study was to investigate how the aging process alters the basal levels of serotonin, norepinephrine, dopamine, and their respective metabolites in the spinal cord using high-performance liquid chromatography and electrochemical detection. Young, mature and aged male Fischer 344 rats (5-6,
Ko M L; King M A; Gordon T L; Crisp T
Brain research bulletin
1997
1905-6
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/s0361-9230(96)00216-x" target="_blank" rel="noreferrer noopener">10.1016/s0361-9230(96)00216-x</a>
The effects of aging on mu and delta opioid receptors in the spinal cord of Fischer-344 rats.
Aging/*metabolism; Ala(2)-MePhe(4)-Gly(5)-; Animals; delta/antagonists & inhibitors/*metabolism; Enkephalin; Enkephalins/metabolism; Inbred F344; Male; mu/agonists/*metabolism; Naltrexone/analogs & derivatives/metabolism; Narcotic Antagonists/metabolism; Opioid; Radioligand Assay; Rats; Receptors; Spinal Cord/*metabolism
Previous research has demonstrated that the antinociceptive efficacy of opioids decreases with advancing age. This study utilized radioligand binding techniques to determine if this decline is due to a change in the receptor density (Bmax) and/or affinity (measured as Kd) of the mu (mu) and/or delta (delta) opioid receptors in the spinal cord with advancing age. Saturation binding analysis with [3H][d-Ala2,N-methyl-Phe4,Gly5-ol]enkephalin (DAMGO: a mu-opioid selective agonist) and [3H]naltrindole (a delta-opioid selective antagonist) revealed no age-related changes in Bmax for either the mu or delta-opioid receptors. The Kd value for naltrindole was likewise unaffected by age. The Kd value for DAMGO however, was significantly higher in the aged group as compared with the young and mature groups, indicating a decreased affinity of spinal mu-opioid receptors for DAMGO.
Hoskins D L; Gordon T L; Crisp T
Brain research
1998
1998-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/s0006-8993(98)00034-1" target="_blank" rel="noreferrer noopener">10.1016/s0006-8993(98)00034-1</a>
Striatal dopamine release in vitro from immature male rats shows enhanced responsiveness to pulsatile, but not continuous, infusions of L-dopa.
3; 4-Dihydroxyphenylacetic Acid/metabolism; Aging/*metabolism; Animals; Corpus Striatum/*metabolism; Dopamine/*metabolism; Inbred Strains; Levodopa/*administration & dosage/pharmacology; Male; Pulsatile Flow; Rats
In the present experiment we compared the effects of continuous versus pulsed infusions of L-beta-3,4,dihydroxyphenylalanine (L-DOPA) upon dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) release in vitro from superfused corpus striatal tissue fragments of immature (25-32 day) and adult (90-120 day) male rats. In response to two pulse infusions of L-DOPA (5 microM) a significantly greater amount of dopamine and DOPAC was released from the striatal fragments of immature vs adult males. In contrast, when L-DOPA was infused continuously throughout the superfusion virtually identical amounts of dopamine and DOPAC were obtained for immature and adult male rats. No differences in postsuperfusion dopamine tissue content were obtained between adult and immature rats following the two pulses or continuous infusion of L-DOPA. These results suggest that the corpus striatum of the immature rat has an enhanced capacity to convert and release dopamine in response to a submaximal pulsatile infusion of L-DOPA.
Dluzen D; McDermott J
Brain research. Developmental brain research
1991
1991-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0165-3806(91)90175-i" target="_blank" rel="noreferrer noopener">10.1016/0165-3806(91)90175-i</a>
Age-related changes in monoamines within the olfactory bulbs of the Fischer 344 male rat.
3; 4-Dihydroxyphenylacetic Acid/metabolism; Aging/*metabolism; Animals; Biogenic Monoamines/*metabolism; Dopamine/metabolism; Inbred F344; Male; Olfactory Bulb/*metabolism; Rats; Serotonin/metabolism
In this report the olfactory bulbs (OB) were removed from 5-6 month, 15-16 month and 25-26 month male Fischer 344 rats and assayed for concentrations of monoamines and their metabolites using HPLC-EC. Concentrations of norepinephrine were significantly greater in 25-26 and 15-16 compared to the 5-6 month old rats. By contrast, the norepinephrine metabolite, 3-methoxy-4-hydroxyphenyl glycol (MHPG), was significantly lower in the 25-26 vs. the 15-16 and 5-6 month old animals. While OB concentrations of dopamine and serotonin did not differ among the three age groups, their respective metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindole-3-acetic acid (5-HIAA), were significantly reduced in the 15-16 and 25-26 month old animals compared to the 5-6 month old animals. These data show some of the age related changes which occur in the monoamines of the OB of the Fischer 344 rat. The salient bi-directional changes which are observed between norepinephrine and its metabolite, MHPG, are particularly intriguing since they reveal some of the mechanistic alterations that occur in the OB noradrenergic system, which may underlie the age related changes in olfactory related memory/recognition processes.
Dluzen D E
Mechanisms of ageing and development
1996
1996-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0047-6374(96)01774-5" target="_blank" rel="noreferrer noopener">10.1016/0047-6374(96)01774-5</a>
The effect of estrogen administration in vivo upon catecholamine release in vitro from superfused hypothalamic tissue of ovariectomized pre-pubertal and adult mice.
*Ovariectomy; Aging/*metabolism; Animals; Catecholamines/*metabolism; Dopamine/metabolism; Estradiol/*pharmacology; Female; Hypothalamus/*drug effects/metabolism; Mice; Norepinephrine/metabolism; Organ Size/drug effects; Potassium/pharmacology; Uterus/drug effects/growth & development
In the present report we examined the effects of estrogen upon catecholamine release from superfused medial basal hypothalamic tissue fragments of pre-pubertal ovariectomized CD-1 mice. Prepubertal mice treated with estradiol benzoate (EB–5 micrograms x 2 days, sc), showed significantly reduced amounts of dopamine but no changes in norepinephrine release in response to a depolarizing concentration of potassium (30 mmol/L) compared with their respective groups receiving the oil vehicle. Since EB treatment reduced potassium stimulated dopamine release in these pre-pubertal mice, in a second experiment we compared the effects of EB versus oil vehicle treatment upon potassium stimulated dopamine release from the hypothalamus of the ovariectomized adult female mouse. Similar to that observed in the pre-pubertal mouse, EB treatment significantly reduced the amount of potassium stimulated dopamine release. Interestingly, the absolute amounts of potassium stimulated dopamine release was substantially greater in adult compared with pre-pubertal mice. These results demonstrate that the hypothalamic dopaminergic system of both pre-pubertal and adult mice show relatively similar responses to estrogen treatment but differ in absolute amounts of dopamine released.
Dluzen D; Attaran M; Liu B
Journal of endocrinological investigation
1994
1994-12
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1007/BF03347791" target="_blank" rel="noreferrer noopener">10.1007/BF03347791</a>
Developmental changes in levels of growth hormone mRNA in Zucker rats.
Aging/*metabolism; Animals; Blotting; Glyceraldehyde-3-Phosphate Dehydrogenases/genetics; Growth Hormone/blood/*genetics; Immunoblotting; Male; Messenger/*metabolism; Northern; Obesity/*genetics; Prolactin/genetics; Rats; RNA; Zucker
Levels of pituitary growth hormone (GH) messenger RNA (mRNA) were compared in groups of genetically obese (fa/fa) and lean (Fa/-) littermate male Zucker rats at four different ages, 3, 5, 9, and 11 weeks, in order to determine the earliest age at which a difference between the two groups could be detected. No difference was seen in three-week-old animals. Five weeks of age was the earliest time at which the level of GH mRNA was significantly decreased in the obese rats; this decrease was present at all subsequent ages. Mean serum growth hormone levels were lower in obese animals at all ages, but the differences were not statistically significant because of the large individual variation associated with the pulsatile nature of GH release. The earliest occurrence of differences in GH mRNA level is later than some of the obesity associated abnormalities present in adipose tissue. The earliest time of the GH mRNA differences can be associated with the time when decreased protein deposition is initially seen in the obese rats. Because of this association, decreased GH mRNA may enhance the development of obesity.
Ahmad I; Steggles A W; Carrillo A J; Finkelstein J A
Journal of cellular biochemistry
1990
1990-05
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1002/jcb.240430106" target="_blank" rel="noreferrer noopener">10.1002/jcb.240430106</a>
Age-related expression patterns of Bag-1 and Bcl-2 in growth plate and articular chondrocytes.
Aging/*metabolism; Animals; Articular/cytology/*metabolism; Carrier Proteins/genetics/*metabolism; Cartilage; Cell Division/physiology; Cells; Chondrocytes/cytology/*metabolism; Cultured; DNA-Binding Proteins; Gene Expression Regulation; Growth Plate/*metabolism; Inbred C57BL; Mice; Proto-Oncogene Proteins c-bcl-2/*metabolism; Tissue Distribution; Transcription Factors
Aging cartilage displays increased chondrocyte apoptosis and decreased responsiveness of chondrocytes to growth factors. The molecular mechanisms responsible for these changes have not been identified. Bag-1 is a Bcl-2-binding protein that promotes cell survival, interacts with a diverse group of cellular proteins, and may integrate multiple pathways involved in controlling cell survival, growth, and phenotype. Bcl-2 is important for maintaining chondrocyte phenotype and delaying terminal differentiation and apoptosis of chondrocytes. Comparatively little is known about the role of Bag-1 in cartilage. Here we show that both growth plate and articular chondrocytes in the mouse express the Bag-1 protein. In the growth plate, Bag-1 expression is prominent in the late proliferative and prehypertrophic chondrocytes, displaying a pattern similar to what has been reported for Bcl-2. Further, the expression of both Bcl-2 and Bag-1 declines with age in the articular cartilage. Growth assays demonstrate that knocking down Bag-1 expression causes a decrease in growth rate. These results suggest that Bag-1 is involved in the regulation of chondrocyte phenotype and cartilage aging.
Kinkel Mary D; Yagi Rieko; McBurney Denise; Nugent Ashleigh; Horton Walter E Jr
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
2004
2004-08
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1002/ar.a.20063" target="_blank" rel="noreferrer noopener">10.1002/ar.a.20063</a>