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Text
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URL Address
<a href="http://doi.org/10.1016/j.ygcen.2012.07.011" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.ygcen.2012.07.011</a>
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Pages
562-572
Issue
3
Volume
178
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Title
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Knockdown Of Leptin A Expression Dramatically Alters Zebrafish Development
Publisher
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General and Comparative Endocrinology
Date
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2012
2012-09
Subject
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atlantic salmon; Auditory; bhlh genes; bone; carp cyprinus-carpio; cell fate specification; central-nervous-system; cloning; danio-rerio; differentiation; Endocrinology & Metabolism; gene; metabolism; n-cadherin; receptor; sensory ganglia; Visual; visual-system
Creator
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Liu Q; Dalman M; Chen Y; Akhter M; Brahmandam S; Patel Y; Lowe J; Thakkar M; Gregory A V; Phelps D; Riley C; Londraville R L
Description
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Using morpholino antisense oligonucleotide (MO) technology, we blocked leptin A or leptin receptor expression in embryonic zebrafish, and analyzed consequences of leptin A knock-down on fish development. Embryos injected with leptin A or leptin receptor MOs (leptin A or leptin receptor morphants) had smaller bodies and eyes, undeveloped inner ear, enlarged pericardial cavity, curved body and/or tail and larger yolk compared to control embryos of the same stages. The defects persisted in 6-9 days old larvae. We found that blocking leptin A function had little effect on the development of early brain (1 day old), but differentiation of both the morphant dorsal brain and retinal cells was severely disrupted in older (2 days old) embryos. Despite the enlarged pericardial cavity, differentiation of cardiac cells appeared to be similar to control embryos. Formation of the morphants' inner ear is also severely disrupted, which corroborates existing reports of leptin receptor expression in inner ear of both zebrafish and mammals. Co-injection of leptin A MO and recombinant leptin results in partial rescue of the wild-type phenotype. Our results suggest that leptin A plays distinct roles in zebrafish development. (c) 2012 Elsevier Inc. All rights reserved.
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<a href="http://doi.org/10.1016/j.ygcen.2012.07.011" target="_blank" rel="noreferrer noopener">10.1016/j.ygcen.2012.07.011</a>
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Journal Article or Conference Abstract Publication
2012
Akhter M
atlantic salmon
Auditory
bhlh genes
Bone
Brahmandam S
carp cyprinus-carpio
cell fate specification
central-nervous-system
Chen Y
Cloning
Dalman M
danio-rerio
differentiation
Endocrinology & Metabolism
gene
General and Comparative Endocrinology
Gregory A V
Journal Article or Conference Abstract Publication
Liu Q
Londraville R L
Lowe J
Metabolism
n-cadherin
Patel Y
Phelps D
Receptor
Riley C
sensory ganglia
Thakkar M
Visual
visual-system