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Text
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<a href="http://doi.org/10.1002/jcb.29168" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcb.29168</a>
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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A novel regulatory role of TRAPPC9 in L-plastin-mediated osteoclast actin ring formation
Publisher
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Journal of Cellular Biochemistry
Date
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2019
2019-08
Subject
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actin ring; cytoskeleton; L-plastin; osteoclast; protein trafficking; TRAPPC9; TRAPPII
Creator
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Hussein Nazar J; Mbimba Thomas; Al-Adlaan Asaad A; Ansari Mohammad Y; Jaber Fatima A; McDermott Scott; Kasumov Takhar; Safadi Fayez F
Description
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Trafficking protein particle complex 9 (TRAPPC9) is a major subunit of the TRAPPII complex. TRAPPC9 has been reported to bind nuclear factor κB kinase subunit β (IKKβ) and NF-kB-inducing kinase (NIK) where it plays a role in the canonical and noncanonical of nuclear factor-κB (NF-kB) signaling pathways, receptively. The role of TRAPPC9 in protein trafficking and cytoskeleton organization in osteoclast (OC) has not been studied yet. In this study, we examined the mRNA expression of TRAPPC9 during OC differentiation. Next, we examined the colocalization of TRAPPC9 with cathepsin-K, known to mediate OC resorption suggesting that TRAPPC9 mediates the trafficking pathway within OC. To identify TRAPPC9 protein partners important for OC-mediated cytoskeleton re-organization, we conducted immunoprecipitation of TRAPPC9 in mature OCs followed by mass spectrometry analysis. Our data showed that TRAPPC9 binds various protein partners. One protein with high recovery rate is L-plastin (LPL). LPL localizes at the podosomes and reported to play a crucial role in actin aggregation thereby actin ring formation and OC function. Although the role of LPL in OC-mediated bone resorption has not fully reported in detail. Here, first, we confirmed the binding of LPL to TRAPPC9 and, then, we investigated the potential regulatory role of TRAPPC9 in LPL-mediated OC cytoskeleton reorganization. We assessed the localization of TRAPPC9 and LPL in OC and found that TRAPPC9 is colocalized with LPL at the periphery of OC. Next, we determined the effect of TRAPPC9 overexpression on LPL recruitment to the actin ring using a viral system. Interestingly, our data showed that TRAPPC9 overexpression promotes the recruitment of LPL to the actin ring when compared with control cultures. In addition, we observed that TRAPPC9 overexpression reorganizes actin clusters/aggregates and regulates vinculin recruitment into the OC periphery to initiate podosome formation.
Identifier
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<a href="http://doi.org/10.1002/jcb.29168" target="_blank" rel="noreferrer noopener">10.1002/jcb.29168</a>
2019
actin ring
Al-Adlaan Asaad A
Ansari Mohammad Y
cytoskeleton
Department of Anatomy & Neurobiology
Hussein Nazar J
Jaber Fatima A
Journal of cellular biochemistry
Kasumov Takhar
L-plastin
Mbimba Thomas
McDermott Scott
NEOMED College of Medicine
osteoclast
protein trafficking
Safadi Fayez F
September 2019 Update
TRAPPC9
TRAPPII
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jcp.27071" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcp.27071</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Autophagy plays an essential role in bone homeostasis.
Publisher
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Journal of cellular physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2019
2019-02
Subject
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osteoclast; autophagosome; autophagy; osteoblast; osteocytes
Creator
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Jaber Fatima A; Khan Nazir M; Ansari Mohammad Y; Al-Adlaan Asaad A; Hussein Nazar J; Safadi Fayez F
Description
An account of the resource
Autophagy is very critical for multiple cellular processes. Autophagy plays a critical role in bone cell differentiation and function.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/jcp.27071" target="_blank" rel="noreferrer noopener">10.1002/jcp.27071</a>
2019
Al-Adlaan Asaad A
Ansari Mohammad Y
autophagosome
Autophagy
Department of Anatomy & Neurobiology
Hussein Nazar J
Jaber Fatima A
Journal of cellular physiology
Khan Nazir M
NEOMED College of Medicine
OSTEOBLAST
osteoclast
osteocytes
Safadi Fayez F