1
40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.ajpath.2013.11.031" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.ajpath.2013.11.031</a>
Pages
697–713
Issue
3
Volume
184
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Mutation in osteoactivin decreases bone formation in vivo and osteoblast differentiation in vitro.
Publisher
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The American journal of pathology
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-03
Subject
The topic of the resource
*Signal Transduction; Alkaline Phosphatase/metabolism; Animals; Apoptosis; Bone and Bones/metabolism/pathology; Cell Differentiation/genetics; Eye Proteins/*genetics; Inbred DBA; Male; Membrane Glycoproteins/*genetics; Mice; Mutation; Newborn; Osteoblasts/cytology/*physiology; Osteocalcin/*genetics; Osteogenesis/*genetics; Phenotype; Receptors; Transforming Growth Factor beta/metabolism
Creator
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Abdelmagid Samir M; Belcher Joyce Y; Moussa Fouad M; Lababidi Suzanne L; Sondag Gregory R; Novak Kimberly M; Sanyurah Afif S; Frara Nagat A; Razmpour Roshanak; Del Carpio-Cano Fabiola E; Safadi Fayez F
Description
An account of the resource
We have previously identified osteoactivin (OA), encoded by Gpnmb, as an osteogenic factor that stimulates osteoblast differentiation in vitro. To elucidate the importance of OA in osteogenesis, we characterized the skeletal phenotype of a mouse model, DBA/2J (D2J) with a loss-of-function mutation in Gpnmb. Microtomography of D2J mice showed decreased trabecular mass, compared to that in wild-type mice [DBA/2J-Gpnmb(+)/SjJ (D2J/Gpnmb(+))]. Serum analysis showed decreases in OA and the bone-formation markers alkaline phosphatase and osteocalcin in D2J mice. Although D2J mice showed decreased osteoid and mineralization surfaces, their osteoblasts were increased in number, compared to D2J/Gpnmb(+) mice. We then examined the ability of D2J osteoblasts to differentiate in culture, where their differentiation and function were decreased, as evidenced by low alkaline phosphatase activity and matrix mineralization. Quantitative RT-PCR analyses confirmed the decreased expression of differentiation markers in D2J osteoblasts. In vitro, D2J osteoblasts proliferated and survived significantly less, compared to D2J/Gpnmb(+) osteoblasts. Next, we investigated whether mutant OA protein induces endoplasmic reticulum stress in D2J osteoblasts. Neither endoplasmic reticulum stress markers nor endoplasmic reticulum ultrastructure were altered in D2J osteoblasts. Finally, we assessed underlying mechanisms that might alter proliferation of D2J osteoblasts. Interestingly, TGF-beta receptors and Smad-2/3 phosphorylation were up-regulated in D2J osteoblasts, suggesting that OA contributes to TGF-beta signaling. These data confirm the anabolic role of OA in postnatal bone formation.
Identifier
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<a href="http://doi.org/10.1016/j.ajpath.2013.11.031" target="_blank" rel="noreferrer noopener">10.1016/j.ajpath.2013.11.031</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Signal Transduction
2014
Abdelmagid Samir M
Alkaline Phosphatase/metabolism
Animals
Apoptosis
Belcher Joyce Y
Bone and Bones/metabolism/pathology
Cell Differentiation/genetics
Del Carpio-Cano Fabiola E
Department of Anatomy & Neurobiology
Eye Proteins/*genetics
Frara Nagat A
Inbred DBA
Lababidi Suzanne L
Male
Membrane Glycoproteins/*genetics
Mice
Moussa Fouad M
Mutation
NEOMED College of Medicine
Newborn
Novak Kimberly M
Osteoblasts/cytology/*physiology
Osteocalcin/*genetics
Osteogenesis/*genetics
Phenotype
Razmpour Roshanak
Receptors
Safadi Fayez F
Sanyurah Afif S
Sondag Gregory R
The American journal of pathology
Transforming Growth Factor beta/metabolism
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jbm.a.30731" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jbm.a.30731</a>
Pages
808–822
Issue
4
Volume
78
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Modified aminosilane substrates to evaluate osteoblast attachment, growth, and gene expression in vitro.
Publisher
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Journal of biomedical materials research. Part A
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-09
Subject
The topic of the resource
*Cell Adhesion; *Cell Division; *Gene Expression; *Silanes/chemistry; Alkaline Phosphatase/metabolism; Animals; Base Sequence; Chick Embryo; Collagen/metabolism; DNA Primers; Osteoblasts/*cytology/enzymology/metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA/isolation & purification/metabolism
Creator
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Siperko L M; Jacquet R; Landis W J
Description
An account of the resource
Bone cell-substrate interactions are important to understand in the design, selection, and surface modification of bone implants. To gain insight into such interactions, substrates designed with surface species approximating the physiological environment of bone matrix were studied. Osteoblasts (Ob) grown on three such surfaces were used to evaluate cell-substrate effects on attachment, growth, and gene expression as compared with controls. Initial surface preparation consisted of coating glass slides with aminopropyltriethoxy silane (APTES), after which the coated slides were modified with collagen-rich extracellular matrix components obtained from normally mineralizing avian tendon: the tripeptide arginine-glycine-aspartic acid (arg-gly-asp), or a precipitate formed from a metastable solution containing inorganic ions normally found in blood (simulated body fluid). Each of the modified substrates, as well as the nonmodified (APTES) control, provided distinctly different physical (evidenced by differences in rms roughness) and chemical surfaces for seeding primary osteoblasts obtained from 14-day-old normal embryonic chickens. Cell responses to each of the substrates were evaluated over a 21-day period in terms of Ob growth and growth rate, alkaline phosphatase (ALP) activity, and gene expression of type I collagen (COL I), osteopontin (OPN), osteocalcin (OC), and bone sialoprotein (BSP). From these preliminary experiments, indications are that cell attachment and growth in this study possibly are independent processes, an assumption that compels the need for further studies. Collagen-rich matrix-modified substrates had a distinct advantage over others when cell growth rate, ALP activity, and gene expression were considered; cells on these substrates exhibited increased ALP activity and enhanced expression of BSP, OPN, and OC when compared with those of cells on APTES controls or other modified substrates. These results indicate that matrix-modified substrates such as those used in this study provide favorable templates for tissue generation, suggesting their potential in the design of surfaces for bone implants.
Identifier
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<a href="http://doi.org/10.1002/jbm.a.30731" target="_blank" rel="noreferrer noopener">10.1002/jbm.a.30731</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Cell Adhesion
*Cell Division
*Gene Expression
*Silanes/chemistry
2006
Alkaline Phosphatase/metabolism
Animals
Base Sequence
Chick Embryo
Collagen/metabolism
DNA Primers
Jacquet R
Journal of biomedical materials research. Part A
Landis W J
Osteoblasts/*cytology/enzymology/metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA/isolation & purification/metabolism
Siperko L M