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Text
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URL Address
<a href="http://doi.org/10.5966/sctm.2013-0157" target="_blank" rel="noreferrer noopener">http://doi.org/10.5966/sctm.2013-0157</a>
Pages
760–767
Issue
6
Volume
3
Dublin Core
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Title
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Functional outcome after anal sphincter injury and treatment with mesenchymal stem cells.
Publisher
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Stem cells translational medicine
Date
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2014
2014-06
Subject
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Female; Time Factors; Animals; Rats; Mesenchymal stem cells; Transfection; Recovery of Function; Fibrosis; *Mesenchymal Stem Cell Transplantation; *Regeneration; Anal Canal/injuries/metabolism/pathology/physiopathology/*surgery; Anal pressures; Anal sphincter; Fecal incontinence; Green Fluorescent Proteins/biosynthesis/genetics; i.v. infusion; Mesenchymal Stem Cells/metabolism; Pressure; Injections; Intralesional; Sprague-Dawley; Cells; Cultured; Animal; Disease Models; Infusions; Intravenous
Creator
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Salcedo Levilester; Penn Marc; Damaser Margot; Balog Brian; Zutshi Massarat
Description
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This research demonstrates the regenerative effects of mesenchymal stem cells (MSCs) on the injured anal sphincter by comparing anal sphincter pressures following intramuscular and serial intravascular MSC infusion in a rat model of anal sphincter injury. Fifty rats were divided into injury (n = 35) and no injury (NI; n = 15) groups. Each group was further divided into i.m., serial i.v., or no-treatment (n = 5) groups and followed for 5 weeks. The injury consisted of an excision of 25% of the anal sphincter complex. Twenty-four hours after injury, 5 x 10(5) green fluorescent protein-labeled MSCs in 0.2 ml of phosphate-buffered saline (PBS) or PBS alone (sham) were injected into the anal sphincter for i.m. treatment; i.v. and sham i.v. treatments were delivered daily for 6 consecutive days via the tail vein. Anal pressures were recorded before injury and 10 days and 5 weeks after treatment. Ten days after i.m. MSC treatment, resting and peak pressures were significantly increased compared with those in sham i.m. treatment (p \textless .001). When compared with the NI group, the injury groups had anal pressures that were not significantly different 5 weeks after i.m./i.v. treatment. Both resting and peak pressures were also significantly increased after i.m./i.v. MSC treatment compared with treatment with PBS (p \textless .001), suggesting recovery. Statistical analysis was done using paired t test with Bonferroni correction. Marked decrease in fibrosis and scar tissue was seen in both MSC-treated groups. Both i.m. and i.v. MSC treatment after injury caused an increase in anal pressures sustained at 5 weeks, although fewer cells were injected i.m. The
Identifier
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<a href="http://doi.org/10.5966/sctm.2013-0157" target="_blank" rel="noreferrer noopener">10.5966/sctm.2013-0157</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Mesenchymal Stem Cell Transplantation
*Regeneration
2014
Anal Canal/injuries/metabolism/pathology/physiopathology/*surgery
Anal pressures
Anal sphincter
Animal
Animals
Balog Brian
Cells
Cultured
Damaser Margot
Disease Models
Fecal incontinence
Female
Fibrosis
Green Fluorescent Proteins/biosynthesis/genetics
i.v. infusion
Infusions
Injections
Intralesional
Intravenous
Mesenchymal stem cells
Mesenchymal Stem Cells/metabolism
Penn Marc
Pressure
Rats
Recovery of Function
Salcedo Levilester
Sprague-Dawley
Stem cells translational medicine
Time Factors
Transfection
Zutshi Massarat