1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.jchromb.2014.05.048" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.jchromb.2014.05.048</a>
Pages
83–89
Volume
963
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Development and validation of an LC-MS/MS method for determination of the L-type voltage-gated calcium channel/NMDA receptor antagonist NGP1-01 in mouse serum.
Publisher
An entity responsible for making the resource available
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-07
Subject
The topic of the resource
Animals; Bridged-Ring Compounds/*blood; Calcium Channel Blockers/*blood; Chromatography; LC-MS/MS; Limit of Detection; Liquid/*methods; Mice; Mouse serum; Multifunctional drug; N-Methyl-D-Aspartate/*antagonists & inhibitors; Neuroprotective agent; NGP1-01; Pentacycloundecylamine; Receptors; Reproducibility of Results; Tandem Mass Spectrometry/*methods
Creator
An entity primarily responsible for making the resource
Jogiraju Harini; Zhou Xiang; Gobburi Ashta Lakshmi Prasad; Pedada Kiran K; Geldenhuys Werner J; Van der Schyf Cornelis J; Crish Samuel D; Anderson David J
Description
An account of the resource
NGP1-01 (8-benzylamino-8,11-oxapentacyclo[5.4.0.0(2,6).0(3,10).0(5,9)]undecane) is a heterocyclic cage compound with multifunctional calcium channel blocking activity that has been demonstrated to be neuroprotective in several neurodegenerative models. A sensitive internal standard LC-MS/MS method was developed and validated to quantify NGP1-01 in mouse serum. The internal standard (IS) was
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.jchromb.2014.05.048" target="_blank" rel="noreferrer noopener">10.1016/j.jchromb.2014.05.048</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2014
Anderson David J
Animals
Bridged-Ring Compounds/*blood
Calcium Channel Blockers/*blood
Chromatography
Crish Samuel D
Department of Pharmaceutical Sciences
Geldenhuys Werner J
Gobburi Ashta Lakshmi Prasad
Jogiraju Harini
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
LC-MS/MS
Limit of Detection
Liquid/*methods
Mice
Mouse serum
Multifunctional drug
N-Methyl-D-Aspartate/*antagonists & inhibitors
NEOMED College of Pharmacy
Neuroprotective agent
NGP1-01
Pedada Kiran K
Pentacycloundecylamine
Receptors
Reproducibility of Results
Tandem Mass Spectrometry/*methods
Van der Schyf Cornelis J
Zhou Xiang
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.jchromb.2013.11.048" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.jchromb.2013.11.048</a>
Pages
141–146
Volume
945-946
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A quantitative LC-MS/MS method for determination of thiazolidinedione mitoNEET ligand NL-1 in mouse serum suitable for pharmacokinetic studies.
Publisher
An entity responsible for making the resource available
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-01
Subject
The topic of the resource
%CV; %RE; 1-methyl-4-phenyl-1; 2; 3; 3-thiazolidine-2; 4-dione; 5-[3; 5-[4-hydroxy-3; 5-di-tert-butyl-4-hydroxyphenyl)methyl]-1; 5-dimethyl-phenyl)methyl]thiazolidine-2; 6-tetrahydropyridine; Animals; Carry-over; Chromatography; High Pressure Liquid/*methods; internal standard; IS; LC-MS/MS; Limit of Detection; LLOQ; lower limit of quantification; Methanol; Mice; mitoNEET; Mouse serum; MPTP; MRM; multiple reaction monitoring; NL-1; NL-2; percent coefficient of variation; peroxisome proliferator activated receptor-gamma; pharmacokinetic; PK; PPAR-gamma; QC; quality control; relative error; relative matrix effect; RME; SD; standard deviation; Tandem Mass Spectrometry/*methods; thiazolidinedione; Thiazolidinedione (TZD); Thiazolidinediones/*blood; TZD
Creator
An entity primarily responsible for making the resource
Pedada Kiran K; Zhou Xiang; Jogiraju Harini; Carroll Richard T; Geldenhuys Werner J; Lin Li; Anderson David J
Description
An account of the resource
Thiazolidinedione (TZD) compounds have shown promise as antidiabetic, antibiotics, antifungal and neuroprotective agents. The mitochondrial effect of a novel mitoNEET ligand, NL-1 {5-[(3,5-di-tert-butyl-4-hydroxyphenyl)methyl]-1,3-thiazolidine-2,4-dione}, and other TZD compounds, is a newly proposed mechanism for the neuroprotective action of these TZD compounds. In this work, a sensitive LC-MS/MS assay has been developed and validated for quantification of NL-1 in mouse serum. Sample preparation involved an acetonitrile protein precipitation procedure with addition of an internal standard NL-2 {5-[(4-hydroxy-3,5-dimethyl-phenyl)methyl]thiazolidine-2,4-dione}. LC-MS/MS analysis utilized a Columbus C-18 HPLC column (2mmx50mm, 5mum). Chromatography employed a multiple step gradient program that featured a steep linear gradient (25-95% in 0.5min) of 15muM ammonium acetate (additive for eliminating carry-over) in 2% methanol mixing with increasing proportions of 100% methanol. The HPLC was interfaced to a QTrap 5500 mass spectrometer (AB Sciex) equipped with an electrospray ionization source used in a negative ionization mode. Multiple reaction monitoring (MRM) of m/z 334–\textgreater263 for NL-1 and m/z 250–\textgreater179 for NL-2 was done. The method had a linear range of at least 1-100ng/mL in serum. The intra-assay and inter-assay percent coefficient of variation (%CV) were less than 4% and accuracies (%RE) ranged from -2.7% to 2.0%. The analytical procedure gave 96-115% absolute extraction recovery of NL-1. The relative matrix effect was measured and found to be insignificant. The analyte in serum was confirmed to be stable during storage and treatment. The method is suitable for pharmacokinetic (PK) studies of the parent drug NL-1 based on the preliminary serum results from dosed NL-1 mouse studies.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.jchromb.2013.11.048" target="_blank" rel="noreferrer noopener">10.1016/j.jchromb.2013.11.048</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
%CV
%RE
1-Methyl-4-phenyl-1
2
2014
3
3-thiazolidine-2
4-dione
5-[3
5-[4-hydroxy-3
5-di-tert-butyl-4-hydroxyphenyl)methyl]-1
5-dimethyl-phenyl)methyl]thiazolidine-2
6-tetrahydropyridine
Anderson David J
Animals
Carroll Richard T
Carry-over
Chromatography
Department of Internal Medicine
Geldenhuys Werner J
High Pressure Liquid/*methods
internal standard
IS
Jogiraju Harini
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
LC-MS/MS
Limit of Detection
Lin Li
LLOQ
lower limit of quantification
Methanol
Mice
mitoNEET
Mouse serum
MPTP
MRM
multiple reaction monitoring
NEOMED College of Medicine
NL-1
NL-2
Pedada Kiran K
percent coefficient of variation
peroxisome proliferator activated receptor-gamma
pharmacokinetic
PK
PPAR-gamma
QC
Quality Control
relative error
relative matrix effect
RME
SD
standard deviation
Tandem Mass Spectrometry/*methods
thiazolidinedione
Thiazolidinedione (TZD)
Thiazolidinediones/*blood
TZD
Zhou Xiang