1
40
2
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s11262-018-1617-5" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s11262-018-1617-5</a>
Pages
68–75
Issue
1
Volume
55
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The large protein 'L' of Peste-des-petits-ruminants virus exhibits RNA triphosphatase activity, the first enzyme in mRNA capping pathway.
Publisher
An entity responsible for making the resource available
Virus Genes
Date
A point or period of time associated with an event in the lifecycle of the resource
2019
2019-02
Subject
The topic of the resource
Acid Anhydride Hydrolases/*metabolism; Animals; Baculoviridae/genetics; Cercopithecus aethiops; Cloning; Conventional mRNA capping; Enzyme Activation; Gene Expression; Genetic Vectors/genetics; Messenger/*genetics/*metabolism; Molecular; Morbillivirus; mRNA capping; Peste-des-petits-ruminants virus L protein; Peste-des-petits-ruminants virus/*physiology; Peste-des-Petits-Ruminants/*virology; PPRV; RNA; RNA Caps/*metabolism; RNA triphosphatase; Vero Cells; Viral Proteins/*metabolism
Creator
An entity primarily responsible for making the resource
Ansari Mohammad Yunus; Singh Piyush Kumar; Rajagopalan Deepa; Shanmugam Purnima; Bellur Asutosh; Shaila Melkote Subbarao
Description
An account of the resource
Peste-des-petits-ruminants is a highly contagious and fatal disease of goats and sheep caused by non-segmented, negative strand RNA virus belonging to the Morbillivirus genus-Peste-des-petits-ruminants virus (PPRV) which is evolutionarily closely related to Rinderpest virus (RPV). The large protein 'L' of the members of this genus is a multifunctional catalytic protein, which transcribes and replicates the viral genomic RNA as well as possesses mRNA capping, methylation and polyadenylation activities; however, the detailed mechanism of mRNA capping by PPRV L protein has not been studied. We have found earlier that the L protein of RPV has RNA triphosphatase (RTPase), guanylyltransferase (GTase) and methyltransferase activities, and unlike vesicular stomatitis virus (VSV), follows the conventional pathway of mRNA capping. In the present work, using a 5'-end labelled viral RNA as substrate, we demonstrate that PPRV L protein has RTPase activity when present in the ribonucleoprotein complex of purified virus as well as recombinant L-P complex expressed in insect cells. Further, a minimal domain in the C-terminal region (aa1640-1840) of the L protein has been expressed in E. coli and shown to exhibit RTPase activity. The RTPase activity of PPRV L protein is metal-dependent and functions with a divalent cation, either magnesium or manganese. In addition, RTPase associated nucleotide triphosphatase activity (NTPase) of PPRV L protein is also demonstrated. This work provides the first detailed study of RTPase activity and identifies the RTPase domain of PPRV L protein.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s11262-018-1617-5" target="_blank" rel="noreferrer noopener">10.1007/s11262-018-1617-5</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2019
Acid Anhydride Hydrolases/*metabolism
Animals
Ansari Mohammad Yunus
Baculoviridae/genetics
Bellur Asutosh
Cercopithecus aethiops
Cloning
Conventional mRNA capping
Department of Anatomy & Neurobiology
Enzyme Activation
Gene Expression
Genetic Vectors/genetics
Messenger/*genetics/*metabolism
Molecular
Morbillivirus
mRNA capping
NEOMED College of Medicine
Peste-des-petits-ruminants virus L protein
Peste-des-petits-ruminants virus/*physiology
Peste-des-Petits-Ruminants/*virology
PPRV
Rajagopalan Deepa
RNA
RNA Caps/*metabolism
RNA triphosphatase
Shaila Melkote Subbarao
Shanmugam Purnima
Singh Piyush Kumar
Vero Cells
Viral Proteins/*metabolism
Virus Genes
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jor.23262" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jor.23262</a>
Pages
311–320
Issue
2
Volume
35
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Harpagoside suppresses IL-6 expression in primary human osteoarthritis chondrocytes.
Publisher
An entity responsible for making the resource available
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-02
Subject
The topic of the resource
*c-FOS/AP-1; *harpagoside; *IL-6; *MMP-13; *osteoarthritis; CCAAT-Enhancer-Binding Protein-beta/metabolism; Chemokines/metabolism; Chondrocytes/*drug effects/metabolism; Drug Evaluation; Glycosides/pharmacology/*therapeutic use; Harpagophytum; Humans; Interleukin-1beta; Interleukin-6/antagonists & inhibitors/*metabolism; Matrix Metalloproteinase 13/metabolism; NF-kappa B/metabolism; Osteoarthritis/*drug therapy/metabolism; Phytotherapy; Plant Extracts/pharmacology/therapeutic use; Preclinical; Primary Cell Culture; Proto-Oncogene Proteins c-fos/metabolism; Pyrans/pharmacology/*therapeutic use; Reactive Oxygen Species/metabolism; Transcription Factor AP-1/metabolism
Creator
An entity primarily responsible for making the resource
Haseeb Abdul; Ansari Mohammad Yunus; Haqqi Tariq M
Description
An account of the resource
There is growing evidence in support of the involvement of inflammatory response in the pathogenesis of osteoarthritis (OA). Harpagoside, one of the bioactive components of Harpagophytum procumbens (Hp), has been shown to possess anti-inflammatory properties. Here we used an in vitro model of inflammation in OA to investigate the potential of harpagoside to suppress the production of inflammatory cytokines/chemokines such as IL-6 and matrix degrading proteases. We further investigated the likely targets of harpagoside in primary human OA chondrocytes. OA chondrocytes were pre-treated with harpagoside before stimulation with IL-1beta. mRNA expression profile of 92 cytokines/chemokines was determined using TaqMan Human Chemokine PCR Array. Expression levels of selected mRNAs were confirmed using TaqMan assays. Protein levels of IL-6 and MMP-13 were assayed by ELISA and immunoblotting. Total protein levels and phosphorylation of signaling proteins were determined by immunoblotting. Cellular localization of
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/jor.23262" target="_blank" rel="noreferrer noopener">10.1002/jor.23262</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*c-FOS/AP-1
*harpagoside
*IL-6
*MMP-13
*OSTEOARTHRITIS
2017
Ansari Mohammad Yunus
CCAAT-Enhancer-Binding Protein-beta/metabolism
Chemokines/metabolism
Chondrocytes/*drug effects/metabolism
Department of Anatomy & Neurobiology
Drug Evaluation
Glycosides/pharmacology/*therapeutic use
Haqqi Tariq M
Harpagophytum
Haseeb Abdul
Humans
Interleukin-1beta
Interleukin-6/antagonists & inhibitors/*metabolism
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Matrix Metalloproteinase 13/metabolism
NEOMED College of Medicine
NF-kappa B/metabolism
Osteoarthritis/*drug therapy/metabolism
Phytotherapy
Plant Extracts/pharmacology/therapeutic use
Preclinical
Primary Cell Culture
Proto-Oncogene Proteins c-fos/metabolism
Pyrans/pharmacology/*therapeutic use
Reactive Oxygen Species/metabolism
Transcription Factor AP-1/metabolism