1
40
9
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0003-9861(90)90664-k" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0003-9861(90)90664-k</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
429-439
Issue
2
Volume
283
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cloning And Characterization Of 2 Major 3-methylcholanthrene Inducible Hamster Liver Cytochrome-p450s
Publisher
An entity responsible for making the resource available
Archives of Biochemistry and Biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-12
Subject
The topic of the resource
Biochemistry & Molecular Biology; Biophysics
Creator
An entity primarily responsible for making the resource
Lai T S; Chiang J Y L
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0003-9861(90)90664-k" target="_blank" rel="noreferrer noopener">10.1016/0003-9861(90)90664-k</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1990
Archives of biochemistry and biophysics
Biochemistry & Molecular Biology
Biophysics
Chiang J Y L
Journal Article or Conference Abstract Publication
Lai T S
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/abbi.2000.2119" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/abbi.2000.2119</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
81-87
Issue
1
Volume
384
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Dublin Core
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Title
A name given to the resource
Cytochrome P450 2e1 (cyp2e1)-dependent Production Of A 37-kda Acetaldehyde-protein Adduct In The Rat Liver
Publisher
An entity responsible for making the resource available
Archives of Biochemistry and Biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-12
Subject
The topic of the resource
acetaldehyde; acetaldehyde-protein adduct; alcohol; alcohol metabolism; aldehyde; Biochemistry & Molecular Biology; Biophysics; CYP2E1; CYP2E1 inhibitor; dehydrogenase; ethanol; expression; hepatocytes; hydroxyethyl radical adducts; induction; lipid-peroxidation; plasma-membrane
Creator
An entity primarily responsible for making the resource
Jeong K S; Soh Y; Jeng J; Felder M R; Hardwick J P; Song B J
Description
An account of the resource
Ethanol-inducible cytochrome P450 2E1 (CYP2E1) has been shown to be involved in the metabolism of both ethanol and acetaldehyde, Acetaldehyde, produced from ethanol metabolism, is highly reactive and can form various protein adducts, In this study, we investigated the role of CYP2E1 in the production of a 37-kDa acetaldehyde-protein adduct, Rats were pair-fed an isocaloric control or an alcohol liquid diet with and without cotreatment of YH439, an inhibitor of CYP2E1 gene transcription, for 4 weeks. The soluble proteins from rat livers of each group were separated on SDS-polyacrylamide gels followed by immunoblot analysis using specific antibodies against the 37-kDa protein acetaldehyde adduct, In addition, catalytic activities of the enzymes involved in alcohol and acetaldehyde metabolism were measured and compared with the adduct level, Immunoblot analysis revealed that the 37-kDa adduct, absent in the pair-fed control, was evident in alcohol-fed rats but markedly reduced by YH439 treatment. Immunohistochemical analysis also showed that the 37-kDa adduct is predominantly localized in the pericentral region of the liver where CYP2E1 protein is mainly expressed. This staining disappeared in the pericentral region after YH439 treatment. The levels of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase isozymes were unchanged after YH439 treatment. However, the level of the 37-kDa protein adduct positively correlated with the hepatic content of P4502E1, These data indicate that the 37-kDa adduct could be produced by CYP2E1-mediated ethanol metabolism in addition to the ADH-dependent formation. (C) 2000 Academic Press.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/abbi.2000.2119" target="_blank" rel="noreferrer noopener">10.1006/abbi.2000.2119</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2000
acetaldehyde
acetaldehyde-protein adduct
Alcohol
alcohol metabolism
aldehyde
Archives of biochemistry and biophysics
Biochemistry & Molecular Biology
Biophysics
CYP2E1
CYP2E1 inhibitor
dehydrogenase
ETHANOL
expression
Felder M R
Hardwick J P
hepatocytes
hydroxyethyl radical adducts
induction
Jeng J
Jeong K S
Journal Article or Conference Abstract Publication
lipid-peroxidation
plasma-membrane
Soh Y
Song B J
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0003-9861(95)90012-8" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0003-9861(95)90012-8</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
289-296
Issue
2
Volume
320
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
FATTY-ACID DISCRIMINATION AND OMEGA-HYDROXYLATION BY CYTOCHROME-P450 4A1 AND A CYTOCHROME P4504A1/NADPH-P450 REDUCTASE FUSION PROTEIN
Publisher
An entity responsible for making the resource available
Archives of Biochemistry and Biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-07
Subject
The topic of the resource
Biochemistry & Molecular Biology; Biophysics; catalytic properties; cytochrome p450 4a1; escherichia-coli; expression; fusion protein; gene family; inhibition; lauric acid; mechanisms; omega-hydroxylation; purification
Creator
An entity primarily responsible for making the resource
Alterman M A; Chaurasia C S; Lu P; Hardwick J P; Hanzlik R P
Description
An account of the resource
The omega-hydroxylation of fatty acids by certain cytochrome P450 enzymes shows a degree of chain-length and regiospecificity which is remarkable in view of the conformational flexibility of these substrates, the strong similarity in properties among homologs, and the lack of polar groups (other than the carboxy terminus) with which to guide and strengthen enzyme-substrate interactions, To investigate the chemical basis for these features of omega-hydroxylation we designed and synthesized a series of lauric acid analogs and evaluated them as substrates and inhibitors of omega-hydroxylation catalyzed by cytochrome P4504A1 and a cytochrome P450 4A1/NADPH-P450 reductase fusion protein, Among n-alkanoic acids, lauric acid was found to have the optimum chain length for the fusion protein, as it does for native cytochrome P450 4A1, With both enzymes, chain shortening caused a precipitous drop in turnover while chain lengthening caused a gradual drop in turnover, The fusion protein omega-hydroxylated methyl laurate and lauryl alcohol about 1/10th as efficiently as lauric acid, but it did not hydroxylate lauramide, 10-Methoxydecanoic acid underwent O-demethylation (via omega-hydroxylation). The branched substrate 11-methyllauric acid was hydroxylated efficiently and selectively at the omega-position, In contrast, the cyclopropyl analog 11,12-methanolauric acid was not detectably hydroxylated, although it induced Type I binding spectrum and inhibited lauric acid omega-hydroxylation by 43% at equimolar concentrations, omega-(Imidazolyl)-decanoic acid induced a Type II heme-binding spectrum and was an especially potent inhibitor of lauric acid hydroxylation, Collectively these data suggest that the active site of cytochrome P450 4A1 has an elongated tubular shape of definite length (ca, 14 Angstrom) with a recognition site for polar groups (including but not limited to carboxyl) at its entrance and the (oxo)heme group at its terminus, (C) 1995 Academic Press, Inc.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0003-9861(95)90012-8" target="_blank" rel="noreferrer noopener">10.1016/0003-9861(95)90012-8</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1995
Alterman M A
Archives of biochemistry and biophysics
Biochemistry & Molecular Biology
Biophysics
catalytic properties
Chaurasia C S
cytochrome p450 4a1
escherichia-coli
expression
fusion protein
gene family
Hanzlik R P
Hardwick J P
inhibition
Journal Article or Conference Abstract Publication
lauric acid
Lu P
mechanisms
omega-hydroxylation
purification
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0003-9861(90)90664-k" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0003-9861(90)90664-k</a>
Pages
429–439
Issue
2
Volume
283
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s.
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-12
Subject
The topic of the resource
Female; Humans; Male; Animals; Rats; Amino Acid Sequence; Reference Values; Base Sequence; Liver/enzymology; Immunoblotting; Molecular Sequence Data; Cricetinae; Mesocricetus; Phenobarbital/pharmacology; Restriction Mapping; Nucleic Acid Hybridization; Cytochrome P-450 Enzyme System/biosynthesis/*genetics; Enzyme Induction; Liver/drug effects/*enzymology; Methylcholanthrene/*pharmacology; Inbred Strains; Sequence Homology; Cloning; Molecular; Nucleic Acid; Microsomes
Creator
An entity primarily responsible for making the resource
Lai T S; Chiang J Y
Description
An account of the resource
We have studied the immunochemical properties of two major 3-methylcholanthrene inducible hamster liver cytochrome P450 isozymes, P450 MC1 and P450 MC4. Immunoblots using specific antibodies against P450 MC1 and P450 MC4 demonstrated that these two P450s were present in very low levels in control hamster livers and were greatly induced by 3-methylcholanthrene treatment. P450 MC1 was immunochemically different from P450 MC4, rat P450c and P450d, and rabbit LM4. The immunorelated polypeptide to P450 MC1 was not present in the control or the
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0003-9861(90)90664-k" target="_blank" rel="noreferrer noopener">10.1016/0003-9861(90)90664-k</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1990
Amino Acid Sequence
Animals
Archives of biochemistry and biophysics
Base Sequence
Chiang J Y
Cloning
Cricetinae
Cytochrome P-450 Enzyme System/biosynthesis/*genetics
Department of Integrative Medical Sciences
Enzyme Induction
Female
Humans
Immunoblotting
Inbred Strains
Lai T S
Liver/drug effects/*enzymology
Liver/enzymology
Male
Mesocricetus
Methylcholanthrene/*pharmacology
Microsomes
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Nucleic Acid
Nucleic Acid Hybridization
Phenobarbital/pharmacology
Rats
Reference Values
Restriction Mapping
Sequence Homology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/S0003-9861(02)00066-8" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/S0003-9861(02)00066-8</a>
Pages
84–93
Issue
1
Volume
402
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Phosphatidylinositide 3-kinase regulates angiotensin II-induced cytosolic phospholipase A2 activity and growth in vascular smooth muscle cells.
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-06
Subject
The topic of the resource
Angiotensin II/*metabolism; Animals; Arachidonic Acid/metabolism; Arachidonic Acids/pharmacology; Blotting; Cells; Chromones/pharmacology; Cultured; Enzyme Inhibitors/pharmacology; Flavonoids/pharmacology; Group IV Phospholipases A2; Male; Mitogen-Activated Protein Kinases/metabolism; Morpholines/pharmacology; Muscle; Phosphatidylinositol 3-Kinases/*physiology; Phospholipases A/*metabolism; Phospholipases A2; Phosphorylation; Rats; Smooth; Sprague-Dawley; Vascular/drug effects/*growth & development; Western
Creator
An entity primarily responsible for making the resource
Silfani Tonous N; Freeman Ernest J
Description
An account of the resource
Angiotensin (Ang) II via the AT(1) receptor acts as a mitogen in vascular smooth muscle cells (VSMC) through stimulation of multiple signaling mechanisms, including tyrosine kinases and mitogen-activated protein kinase (MAPK). In addition, cytosolic phospholipase A(2)(cPLA(2))-dependent release of arachidonic acid (AA) is linked to VSMC growth and we have reported that Ang II stimulates cPLA(2) activity via the AT(1) receptor. The coupling of Ang II to the activation of cPLA(2) appears to involve mechanisms both upstream and downstream of MAPK such that AA stimulates MAPK activity which phosphorylates cPLA(2) to further enhance AA release. However, the upstream mechanisms responsible for activation of cPLA(2) are not well-defined. One possibility includes phosphatidylinositide
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/S0003-9861(02)00066-8" target="_blank" rel="noreferrer noopener">10.1016/S0003-9861(02)00066-8</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2002
Angiotensin II/*metabolism
Animals
Arachidonic Acid/metabolism
Arachidonic Acids/pharmacology
Archives of biochemistry and biophysics
Blotting
Cells
Chromones/pharmacology
Cultured
Enzyme Inhibitors/pharmacology
Flavonoids/pharmacology
Freeman Ernest J
Group IV Phospholipases A2
Male
Mitogen-Activated Protein Kinases/metabolism
Morpholines/pharmacology
Muscle
Phosphatidylinositol 3-Kinases/*physiology
Phospholipases A/*metabolism
Phospholipases A2
Phosphorylation
Rats
Silfani Tonous N
Smooth
Sprague-Dawley
Vascular/drug effects/*growth & development
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.abb.2008.01.018" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.abb.2008.01.018</a>
Pages
1–16
Issue
1
Volume
472
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Genomic structure and regulation of the rat hepatic CYP4F1 gene by peroxisome proliferators.
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
2008
2008-04
Subject
The topic of the resource
Animals; Cell Line; Chromosome Mapping; Cytochrome P-450 Enzyme System/*genetics; Gene Expression Regulation/drug effects/*genetics; Genetic/*genetics; Hepatocytes/drug effects/*physiology; Liver/drug effects/*physiology; Peroxisome Proliferator-Activated Receptors/*genetics; Peroxisome Proliferators/*pharmacology; Promoter Regions; Rats
Creator
An entity primarily responsible for making the resource
Donelson Ellen; Chen Liping; Zhang Xiaolan; Goswami Puja; Song Byoung J; Hardwick James P
Description
An account of the resource
The rat hepatic gene CYP4F1 encodes a fatty acid omega hydroxylase P450 that metabolizes proinflammatory eicosanoids and long-chain fatty acids. We have completely sequenced the CYP4F1 gene (Accession Nos. AF200361 and AF181083), identified multiple transcription start sites, and characterized a strong core promoter region, -760/116, induced by retinoic acids and peroxisome proliferators in rat hepatoma McA-RH7777 cells. Three peroxisome proliferator responsive elements (PPRE) bind both PPARalpha/RXRalpha and HNF4alpha. Co-transfection of McA-RH7777 cells with the -760/116 reporter construct and PPARalpha/RXRalpha or HNF4alpha showed that HNF4alpha activated while PPARalpha/RXRalpha inhibited CYP4F1 promoter activity. Treating cells with Wy14,643 reversed all initial effects, indicating co-regulation of CYP4F1 gene transcription by PPARalpha/RXRalpha and HNF4alpha. Chromatin immunoprecipitation analysis of cells treated with Wy14,643 showed association of PPARalpha/RXRalpha with the active transcription of the CYP4F1 gene while in clofibrate treated rats HNF4alpha binds during gene repression, suggesting differential regulation of the CYP4F1 gene in vivo and in cell lines.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.abb.2008.01.018" target="_blank" rel="noreferrer noopener">10.1016/j.abb.2008.01.018</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2008
Animals
Archives of biochemistry and biophysics
Cell Line
Chen Liping
Chromosome Mapping
Cytochrome P-450 Enzyme System/*genetics
Department of Integrative Medical Sciences
Donelson Ellen
Gene Expression Regulation/drug effects/*genetics
Genetic/*genetics
Goswami Puja
Hardwick James P
Hepatocytes/drug effects/*physiology
Liver/drug effects/*physiology
NEOMED College of Medicine
Peroxisome Proliferator-Activated Receptors/*genetics
Peroxisome Proliferators/*pharmacology
Promoter Regions
Rats
Song Byoung J
Zhang Xiaolan
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/abbi.2000.1836" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/abbi.2000.1836</a>
Pages
364–376
Issue
2
Volume
378
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Promoter activity and regulation of the CYP4F2 leukotriene B(4) omega-hydroxylase gene by peroxisomal proliferators and retinoic acid in HepG2 cells.
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-06
Subject
The topic of the resource
*Gene Expression Regulation; *Promoter Regions; Amino Acid Sequence; Base Sequence; Cell Line; Cloning; Cytochrome P-450 CYP4A; Cytochrome P-450 Enzyme System/*genetics/metabolism; Cytochrome P450 Family 4; Cytoplasmic and Nuclear/metabolism; DNA; DNA Footprinting; Enzymologic; Exons; Genes; Genetic; Genetic/drug effects; Humans; Introns; Leukotriene B4/metabolism; Mixed Function Oxygenases/metabolism; Models; Molecular; Molecular Sequence Data; Peroxisome Proliferators/*metabolism; Receptors; Reporter; Retinoic Acid Receptor alpha; Retinoic Acid/metabolism; Sequence Analysis; Transcription; Transcription Factors/metabolism; Transfection; Tretinoin/*metabolism
Creator
An entity primarily responsible for making the resource
Zhang X; Chen L; Hardwick J P
Description
An account of the resource
The human liver CYP4F2 gene (Accession No. AF221943) encodes a leukotriene B(4) omega-hydroxylase that metabolizes leukotriene B(4) (LTB(4)) to a less potent proinflammatory eicosanoid, 20-OH-LTB(4). We sequenced a 6.7-kb genomic fragment of the human CYP4F2 gene that has the first five exons and 500 bp of the 5'-flanking region. The major transcription start site was found to be 49 bp upstream of the 3' end of exon 1 and the ATG translation initiation codon was located in exon 2. Besides the TATA box at -39 bp and basal transcription factor binding sites, the promoter region and 412-bp intron 1 have several putative binding sites for nuclear factors that may mediate the inflammatory response and lipid homeostasis. We found two DR1 elements in the 5' promoter, a DR2 element in intron 1, and RXR/RAR binding sites in both intron 1 and the 5' promoter. DNase I footprinting revealed three protected sequences, with the region containing two CAATT boxes at -71 and -111 bp important in CYP4F2 gene expression. Luciferase reporter assays showed that the 500-bp upstream sequence has strong promoter activity. Transient transfection experiments identified two sites in the 5' promoter and intron 1 that cooperate in gene transcription while exon 1 and a
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/abbi.2000.1836" target="_blank" rel="noreferrer noopener">10.1006/abbi.2000.1836</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
*Promoter Regions
2000
Amino Acid Sequence
Archives of biochemistry and biophysics
Base Sequence
Cell Line
Chen L
Cloning
Cytochrome P-450 CYP4A
Cytochrome P-450 Enzyme System/*genetics/metabolism
Cytochrome P450 Family 4
Cytoplasmic and Nuclear/metabolism
Department of Integrative Medical Sciences
DNA
DNA Footprinting
Enzymologic
Exons
Genes
Genetic
Genetic/drug effects
Hardwick J P
Humans
Introns
Leukotriene B4/metabolism
Mixed Function Oxygenases/metabolism
Models
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Peroxisome Proliferators/*metabolism
Receptors
Reporter
Retinoic Acid Receptor alpha
Retinoic Acid/metabolism
Sequence Analysis
Transcription
Transcription Factors/metabolism
Transfection
Tretinoin/*metabolism
Zhang X
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/abbi.1993.1537" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/abbi.1993.1537</a>
Pages
451–460
Issue
2
Volume
306
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Genomic cloning, sequencing, and analysis of the hamster cholesterol 7 alpha-hydroxylase gene (CYP7).
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-11
Subject
The topic of the resource
Amino Acid; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cholesterol 7-alpha-Hydroxylase/*genetics; Cloning; Cricetinae; DNA/genetics/isolation & purification; Genomic Library; Humans; Liver/enzymology; Mesocricetus/*genetics; Messenger/biosynthesis/metabolism; Molecular; Molecular Sequence Data; Northern; Poly A/biosynthesis/metabolism; Rats; Restriction Mapping; RNA; Sequence Homology; Southern
Creator
An entity primarily responsible for making the resource
Crestani M; Galli G; Chiang J Y
Description
An account of the resource
Cholesterol 7 alpha-hydroxylase is the rate limiting enzyme in bile acid biosynthesis and plays an important role in cholesterol homeostasis. The Golden Syrian hamster has been used as an animal model for the study of atherosclerosis and cholesterol gallstone disease. We have screened a lambda DASH II hamster liver genomic library using a rat cDNA as a hybridization probe. A 14-kb genomic clone has been isolated and characterized by restriction mapping and Southern blot hybridization. The clone contained the full-length gene encoding cholesterol 7 alpha-hydroxylase together with an upstream sequence of approximately 5 kb. DNA sequencing and analysis of about 11 kb of the gene revealed that the hamster CYP7 gene consists of six exons and five introns, which have the same structures and sizes as predicted in the rat and human CYP7 genes. The nucleotide and deduced amino acid sequences of the hamster cholesterol 7 alpha-hydroxylase have a high sequence identity of about 90% to the rat and 82% to the human sequences. Particularly, exons 2, 5, and 6 are highly conserved among these species, thus reflecting the presence of some domains that are crucial for the activity of this unique enzyme. The putative cholesterol-binding region, an aromatic amino acid region, and the P450 heme-binding region are completely conserved. Comparison of the 250-bp 5'-flanking sequence to the corresponding region in the rat and human genes revealed a high degree of homology ranging between 71% and 82%. Next to the canonical TATA and CCAAT boxes are many consensus sequences (LF-A1, LF-B1, TGT3) for liver-specific or -enriched transcription factors (HNF4, HNF1, and HNF5, respectively) and an imperfect direct repeat of thyroid hormone responsive element (TRE), which is located between TGT3 and LF-B1. These sequence motifs are completely conserved among the rat, human, and hamster CYP7 genes. Several modified sterol regulatory element (SRE)-like sequences are located in the upstream flanking region and in the first intron. This highly conserved proximal promoter may play important roles in the transcription activity and in the regulation of the CYP7 gene by physiological agents, such as bile acids and steroid/thyroid hormones. This is the first report describing the complete nucleotide sequence and confirming the structure of a CYP7 gene.(ABSTRACT TRUNCATED AT 400 WORDS)
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/abbi.1993.1537" target="_blank" rel="noreferrer noopener">10.1006/abbi.1993.1537</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
Amino Acid
Amino Acid Sequence
Animals
Archives of biochemistry and biophysics
Base Sequence
Blotting
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cloning
Crestani M
Cricetinae
Department of Integrative Medical Sciences
DNA/genetics/isolation & purification
Galli G
Genomic Library
Humans
Liver/enzymology
Mesocricetus/*genetics
Messenger/biosynthesis/metabolism
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Northern
Poly A/biosynthesis/metabolism
Rats
Restriction Mapping
RNA
Sequence Homology
Southern
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Text
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URL Address
<a href="http://doi.org/10.1006/abbi.1993.1003" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/abbi.1993.1003</a>
Pages
18–23
Issue
1
Volume
300
Dublin Core
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Title
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Identification of a new P450 subfamily, CYP4F1, expressed in rat hepatic tumors.
Publisher
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Archives of biochemistry and biophysics
Date
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1993
1993-01
Subject
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2-Acetylaminofluorene; Aflatoxin B1; Amino Acid; Amino Acid Sequence; Animals; Antisense Elements (Genetics); Base Sequence; Blotting; Clofibrate; Cytochrome P-450 Enzyme System/analysis/*genetics; Humans; Liver Neoplasms/chemically induced/*enzymology/genetics; Male; Microsomes/enzymology; Molecular Sequence Data; Multigene Family; Northern; Rats; Sequence Homology; Western
Creator
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Chen L; Hardwick J P
Description
An account of the resource
The expression of the rat cytochrome P450 CYP4 family was studied in hepatic tumors. In most of the primary and transplantable hepatic tumors studied, lauric acid omega-hydroxylase activity associated with the CYP4A subfamily enzymes decreased. The expression of CYP4A proteins and mRNAs in these tumors as assessed by Western and Northern blot was undetectable. However, while RNA analysis revealed the absence of 4A1, 4A2, and 4A3 mRNAs, the expression of CYP4 gene(s) was detected. A Uni-ZAP cDNA library was constructed from a
Identifier
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<a href="http://doi.org/10.1006/abbi.1993.1003" target="_blank" rel="noreferrer noopener">10.1006/abbi.1993.1003</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
2-Acetylaminofluorene
Aflatoxin B1
Amino Acid
Amino Acid Sequence
Animals
Antisense Elements (Genetics)
Archives of biochemistry and biophysics
Base Sequence
Blotting
Chen L
Clofibrate
Cytochrome P-450 Enzyme System/analysis/*genetics
Department of Integrative Medical Sciences
Hardwick J P
Humans
Liver Neoplasms/chemically induced/*enzymology/genetics
Male
Microsomes/enzymology
Molecular Sequence Data
Multigene Family
NEOMED College of Medicine
Northern
Rats
Sequence Homology
Western