1
40
4
-
Text
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URL Address
<a href="http://doi.org/10.1089/1076327041348527" target="_blank" rel="noreferrer noopener">http://doi.org/10.1089/1076327041348527</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
673-687
Issue
5
Volume
10
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Dublin Core
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Title
A name given to the resource
Tissue Engineering Of An Auricular Cartilage Model Utilizing Cultured Chondrocyte-poly(l-lactide-epsilon-caprolactone) Scaffolds
Publisher
An entity responsible for making the resource available
Tissue Engineering
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-05
Subject
The topic of the resource
articular-cartilage; Cell Biology; cells; chondrocytes; construct; copolymers; laser capture microdissection; morphogenesis; polymer matrices; reconstruction; total; transplantation
Creator
An entity primarily responsible for making the resource
Isogai N; Asamura S; Higashi T; Ikada Y; Morita S; Hillyer J; Jacquet R; Landis W J
Description
An account of the resource
To determine the potential development in vivo of tissue-engineered auricular cartilage, chondrocytes from articular cartilage of bovine forelimb joints were seeded on poly(L-lactic acid-epsilon-caprolactone) copolymer scaffolds molded into the shape of a human ear. Copolymer scaffolds alone in the same shape were studied for comparison. Chondrocyte-seeded copolymer constructs and scaffolds alone were each implanted in dorsal skin flaps of athymic mice for up to 40 weeks. Retrieved specimens were examined by histological and molecular techniques. After 10 weeks of implantation, cell-seeded constructs developed cartilage as assessed by toluidine blue and safranin-O red staining; a vascular, perichondrium-like capsule enveloped these constructs; and tissue formation resembled the auricular shape molded originally. Cartilage matrix formation increased, the capsule persisted, and initial auricular configuration was maintained through implantation for 40 weeks. The presence of cartilage production was correlated with RT-PCR analysis, which showed expression of bovine-specific type II collagen and aggrecan mRNA in cell-seeded specimens at 20 and 40 weeks. Copolymer scaffolds monitored only for 40 weeks failed to develop cartilage or a defined capsule and expressed no mRNA. Extensive vascularization led to scaffold erosion, decrease in original size, and loss of contour and shape. These results demonstrate that poly(L-lactic acid-epsilon-caprolactone) copolymer seeded with articular chondrocytes supports development and maintenance of cartilage in a human ear shape over periods to 40 weeks in this implantation model.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1089/1076327041348527" target="_blank" rel="noreferrer noopener">10.1089/1076327041348527</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2004
articular-cartilage
Asamura S
Cell Biology
Cells
Chondrocytes
construct
copolymers
Higashi T
Hillyer J
Ikada Y
Isogai N
Jacquet R
Journal Article or Conference Abstract Publication
Landis W J
laser capture microdissection
Morita S
Morphogenesis
polymer matrices
reconstruction
Tissue Engineering
total
Transplantation
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1089/1076327041887862" target="_blank" rel="noreferrer noopener">http://doi.org/10.1089/1076327041887862</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1204-1213
Issue
7
Volume
10
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Title
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Characterization of the cellular origin of a tissue-engineered human phalanx model by in situ hybridization
Publisher
An entity responsible for making the resource available
Tissue Engineering
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-07
Subject
The topic of the resource
cells; Cell Biology; expression; endothelial growth-factor; cartilage; joints
Creator
An entity primarily responsible for making the resource
Chubinskaya S; Jacquet R; Isogai N; Asamura S; Landis W J
Description
An account of the resource
Tissue-engineered models of human phalanges have previously been fabricated from a combination of bovine periosteum, cartilage, tendon, and biodegradable polyglycolic acid and poly-L-lactic acid scaffolds. Resulting constructs implanted in athymic mice for more than 40 weeks developed new bone, cartilage, and tendon and became vascularized, but cell types comprising the constructs were unidentified. The origin of cells in middle phalanx models implanted for 20 weeks in nude mice has been studied by in situ hybridization analyzing species-specific gene expression. Oligonucleotide probes homologous to species-specific gene sequences of bovine type 11 and X collagen, aggrecan, bone sialoprotein, biglycan, and osteopontin, and mouse decorin were labeled with S-35 and hybridized to respective serial sections of bovine tissue, mouse tissue, and phalanx constructs. In situ hybridization showed positive message and tissue-specific localization for all bovine-specific probes examined within cartilaginous and midshaft portions of constructs and negative message for the mouse-specific decorin probe. These data show that osteoblasts and chondrocytes comprising constructs are derived exclusively from their original bovine sources over 20 weeks of implantation. Defining the cellular origin of the models lends insight into their biological, chemical, and physical nature and their growth and development. Maintenance of their initial genotype is crucial for future application of the models in augmenting impaired human phalanges and related tissues.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1089/1076327041887862" target="_blank" rel="noreferrer noopener">10.1089/1076327041887862</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2004
Asamura S
Cartilage
Cell Biology
Cells
Chubinskaya S
endothelial growth-factor
expression
Isogai N
Jacquet R
joints
Journal Article or Conference Abstract Publication
Landis W J
Tissue Engineering
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.bone.2005.08.025" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.bone.2005.08.025</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
350-358
Issue
3
Volume
38
Search for Full-text
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Title
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Non-destructive studies of tissue-engineered phalanges by magnetic resonance microscopy and X-ray microtomography
Publisher
An entity responsible for making the resource available
Bone
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-03
Subject
The topic of the resource
bone; bone-mineral density; cartilage; computed-tomography; computed-tomography; Endocrinology & Metabolism; in-vivo; macromolecules; magnetic resonance microscopy; microct; mr; quantitative; relaxation; scaffolds; tissue engineering; trabecular bone; X-ray microtomography
Creator
An entity primarily responsible for making the resource
Potter K; Sweet D E; Anderson P; Davis G R; Isogai N; Asamura S; Kusuhara H; Landis W J
Description
An account of the resource
One of the intents of tissue engineering is to fabricate biological materials for the augmentation or replacement of impaired, damaged, or diseased human tissue. In this context, novel models of the human phalanges have been developed recently through suturing of polymer scaffolds supporting osteoblasts, chondrocytes, and tenocytes to mimic bone, cartilage, and tendon, respectively. Characterization of the model constructs has been accomplished previously through histological and biochemical means, both of which are necessarily destructive to the constructs. This report describes the application of two complementary, non-destructive, non-invasive techniques, magnetic resonance microscopy (MRM) and X-ray microtomography (XMT or quantitative computed tomography), to evaluate the spatial and temporal growth and developmental status of tissue elements within tissue-engineered constructs obtained after 10 and 38 weeks of implantation in athymic (nude) mice. These two times represent respective points at which model middle phalanges are comprised principally of organic components while being largely unmineralized and later become increasingly more mineralized. The spatial distribution of mineralized deposits within intact constructs was readily detected by XMT (qCT) and was comparable to low intensity zones observed on MRM hydration maps. Moreover, the MRM-derived hydration values for mineralized zones were inversely correlated with mineral densities measured by XMT. In addition, the MRM method successfully mapped fat deposits, collagenous tissues, and the hydration state of the soft tissue elements comprising the specimens. These results support the application of non-destructive, non-invasive, quantitative MRM and XMT for the evaluation of constituent tissue elements within complex constructs of engineered implants. (c) 2005 Elsevier Inc. All rights reserved.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.bone.2005.08.025" target="_blank" rel="noreferrer noopener">10.1016/j.bone.2005.08.025</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2006
Anderson P
Asamura S
Bone
bone-mineral density
Cartilage
computed-tomography
Davis G R
Endocrinology & Metabolism
in-vivo
Isogai N
Journal Article
Kusuhara H
Landis W J
macromolecules
magnetic resonance microscopy
microct
mr
Potter K
quantitative
relaxation
scaffolds
Sweet D E
Tissue Engineering
trabecular bone
X-Ray Microtomography
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1111/j.1601-6343.2005.00353.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1111/j.1601-6343.2005.00353.x</a>
Pages
303–312
Issue
4
Volume
8
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Design and assessment of a tissue-engineered model of human phalanges and a small joint.
Publisher
An entity responsible for making the resource available
Orthodontics & craniofacial research
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-11
Subject
The topic of the resource
*Bioartificial Organs; *Biomimetic Materials; *Finger Joint; *Finger Phalanges; *Tissue Engineering; Animals; Biological; Bone and Bones; Cartilage; Cattle; Humans; Lactic Acid; Mice; Models; Nude; Polyglycolic Acid; Polylactic Acid-Polyglycolic Acid Copolymer; Polymers; Tendons
Creator
An entity primarily responsible for making the resource
Landis W J; Jacquet R; Hillyer J; Lowder E; Yanke A; Siperko L; Asamura S; Kusuhara H; Enjo M; Chubinskaya S; Potter K; Isogai N
Description
An account of the resource
OBJECTIVES: To develop models of human phalanges and small joints by suturing different cell-polymer constructs that are then implanted in athymic (nude) mice. DESIGN: Models consisted of bovine periosteum, cartilage, and/or tendon cells seeded onto biodegradable polymer scaffolds of either polyglycolic acid (PGA) or copolymers of PGA and poly-L-lactic acid (PLLA) or poly-epsilon-caprolactone (PCL) and PLLA. Constructs were fabricated to produce a distal phalanx, middle phalanx, or distal interphalangeal joint. SETTING AND SAMPLE POPULATION: Studies of more than 250 harvested implants were conducted at the Northeastern Ohio Universities College of Medicine. EXPERIMENTAL VARIABLE: Polymer scaffold, cell type, and implantation time were examined. OUTCOME MEASURE: Tissue-engineered specimens were characterized by histology, transmission electron microscopy, in situ hybridization, laser capture microdissection and qualitative and quantitative polymerase chain reaction analysis, magnetic resonance microscopy, and X-ray microtomography. RESULTS: Over periods to 60 weeks of implantation, constructs developed through vascularity from host mice; formed new cartilage, bone, and/or tendon; expressed characteristic genes of bovine origin, including type I, II and X collagen, osteopontin, aggrecan, biglycan, and bone sialoprotein; secreted corresponding proteins; responded to applied mechanical stimuli; and maintained shapes of human phalanges with small joints. CONCLUSION: Results give insight into construct processes of tissue regeneration and development and suggest more complete tissue-engineered cartilage, bone, and tendon models. These should have significant future scientific and clinical applications in medicine, including their use in plastic surgery, orthopaedics, craniofacial reconstruction, and teratology.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1111/j.1601-6343.2005.00353.x" target="_blank" rel="noreferrer noopener">10.1111/j.1601-6343.2005.00353.x</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Bioartificial Organs
*Biomimetic Materials
*Finger Joint
*Finger Phalanges
*Tissue Engineering
2005
Animals
Asamura S
Biological
Bone and Bones
Cartilage
Cattle
Chubinskaya S
Enjo M
Hillyer J
Humans
Isogai N
Jacquet R
Kusuhara H
Lactic Acid
Landis W J
Lowder E
Mice
Models
Nude
Orthodontics & craniofacial research
Polyglycolic Acid
Polylactic Acid-Polyglycolic Acid Copolymer
Polymers
Potter K
Siperko L
Tendons
Yanke A