1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
159–173
Issue
1
Volume
9
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Scanning electron microscopy and transmission electron microscopy aspects of synergistic antitumor activity of vitamin C - vitamin K3 combinations against human prostatic carcinoma cells.
Publisher
An entity responsible for making the resource available
Scanning microscopy
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-03
Subject
The topic of the resource
Humans; Male; Antineoplastic Agents/*pharmacology; Drug Synergism; Coloring Agents; Ascorbic Acid/*pharmacology; Carcinoma/*drug therapy/pathology; Cell Membrane/drug effects/ultrastructure; Cytoskeleton/drug effects/ultrastructure; Mitochondria/drug effects/ultrastructure; Prostatic Neoplasms/*drug therapy/pathology; Tetrazolium Salts; Thiazoles; Vitamin K/*pharmacology; Microscopy; Electron; Cultured; Scanning; Tumor Cells; Drug Therapy; Combination
Creator
An entity primarily responsible for making the resource
Gilloteaux J; Jamison J M; Venugopal M; Giammar D; Summers J L
Description
An account of the resource
A MTT/formazan assay was used to evaluate the antitumor activity of vitamin C (Vit C), vitamin K3 (Vit K3), or vitamin C:vitamin K3 combinations against a human prostatic carcinoma cell line (DU145). Both Vit C and Vit K3 alone exhibited antitumor activity, but only at elevated doses. When Vit C and Vit K3 were combined at a C:K3 ratio of 100:1 and administered to the carcinoma cells, the 50% cytotoxic concentrations (CD50) of the vitamins decreased 10- to 60-fold. Subsequently, the DU145 cells were examined with transmission and scanning electron microscopy (TEM and SEM) following a 1 hour treatment with Vit C, Vit K3, or Vit C/K3 combined at their 50% cytotoxic dose. Our morphological data suggest that vitamin treatment with individual vitamins affects the cytoskeleton, the mitochondria, and other membranous components of the cell. Treatment with the vitamin combination appears to potentiate the effects of the individual vitamin treatment. Specifically, there are abundant necrotic cells. The surviving cells display morphological defects characteristic of cell injury.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1995
Antineoplastic Agents/*pharmacology
Ascorbic Acid/*pharmacology
Carcinoma/*drug therapy/pathology
Cell Membrane/drug effects/ultrastructure
Coloring Agents
Combination
Cultured
Cytoskeleton/drug effects/ultrastructure
Drug Synergism
Drug Therapy
Electron
Giammar D
Gilloteaux J
Humans
Jamison J M
Male
Microscopy
Mitochondria/drug effects/ultrastructure
Prostatic Neoplasms/*drug therapy/pathology
Scanning
Scanning microscopy
Summers J L
Tetrazolium Salts
Thiazoles
Tumor Cells
Venugopal M
Vitamin K/*pharmacology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.bcp.2003.08.040" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.bcp.2003.08.040</a>
Pages
337–351
Issue
2
Volume
67
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cell cycle arrest and autoschizis in a human bladder carcinoma cell line following Vitamin C and Vitamin K3 treatment.
Publisher
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Biochemical pharmacology
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-01
Subject
The topic of the resource
Antioxidants/*pharmacology; Ascorbic Acid/*pharmacology; Calcium/metabolism; Cell Cycle/*drug effects; Cultured; DNA; Flow Cytometry; Humans; Hydrogen Peroxide/metabolism; Male; Neoplasm/drug effects; Sulfhydryl Compounds/metabolism; Tumor Cells; Urinary Bladder Neoplasms/pathology; Vitamin K 3/*pharmacology
Creator
An entity primarily responsible for making the resource
Jamison James M; Gilloteaux Jacques; Nassiri M Reza; Venugopal Meenakshi; Neal Deborah R; Summers Jack L
Description
An account of the resource
Exponentially growing cultures of human bladder tumor cells (T24) were treated with Vitamin C (VC) alone, Vitamin K(3) (VK(3)) alone, or with a VC:VK(3) combination for 1, 2, or 4hr. Flow cytometry of T24 cells exposed to the vitamins for 1h revealed a growth arrested population and a population undergoing cell death. Cells in G(1) during vitamin treatment arrested in G(1) while those in S phase progressed through S phase and arrested in G(2)/M. DNA synthesis decreased to 14 to 21% of control levels which agreed with the percent of cells in S phase during treatment. Annexin V labeling demonstrated the majority of the cells died by autoschizis, but necrosis and apoptosis also were observed. Catalase treatment abrogated both cell cycle arrest and cell death which implicated hydrogen peroxide (H(2)O(2)) in these processes. Redox cycling of VC and VK(3) increased H(2)O(2) production and decreased cellular thiol levels and DNA content, while increasing intracellular Ca(2+) levels and lipid peroxidation. Feulgen staining of treated cells revealed a time-dependent decrease in tumor cell DNA, while electrophoresis revealed a spread pattern. These results suggest that Ca(2+) disregulation activates at least one DNase which degrades tumor cell DNA and induces tumor cell death.
Identifier
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<a href="http://doi.org/10.1016/j.bcp.2003.08.040" target="_blank" rel="noreferrer noopener">10.1016/j.bcp.2003.08.040</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2004
Antioxidants/*pharmacology
Ascorbic Acid/*pharmacology
Biochemical pharmacology
Calcium/metabolism
Cell Cycle/*drug effects
Cultured
DNA
Flow Cytometry
Gilloteaux Jacques
Humans
Hydrogen Peroxide/metabolism
Jamison James M
Male
Nassiri M Reza
Neal Deborah R
Neoplasm/drug effects
Sulfhydryl Compounds/metabolism
Summers Jack L
Tumor Cells
Urinary Bladder Neoplasms/pathology
Venugopal Meenakshi
Vitamin K 3/*pharmacology