Osteoactivin, Mmp And Hsp70 Expression In Skeletal Muscle In A Rat Model Of Repetitive Loading
Cell Biology
Frara N; Abdelmagid S M; Tytell M; Popoff S N; Safadi F F; Barbe M F
Molecular Biology of the Cell
2013
2013
Journal Article or Conference Abstract Publication
n/a
Growth And Repair Factors, Osteoactivin, Matrix Metalloproteinase And Heat Shock Protein 72, Increase With Resolution Of Inflammation In Musculotendinous Tissues In A Rat Model Of Repetitive Grasping
carpal-tunnel-syndrome; disorders; endothelial-cells; extracellular-matrix; gene-expression; Heat shock protein; human fibroblasts; Metalloproteinases; muscle; musculoskeletal; Orthopedics; Osteoactivin; Overuse; posttranscriptional; regulation; Restorative repair; Rheumatology; skeletal-muscle regeneration; Tendon; transgenic expression; upper extremity
Frara N; Abdelmagid S M; Tytell M; Amin M; Popoff S N; Safadi F F; Barbe M F
Bmc Musculoskeletal Disorders
2016
2016-01
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1186/s12891-016-0892-3" target="_blank" rel="noreferrer noopener">10.1186/s12891-016-0892-3</a>
Osteoactivin, Inducible Heat Shock Protein 70 And Transforming Growth Factor Beta 1 Expression In Skeletal Muscle Of High Repetitive High Force Reaching Rats In Association With Anti-inflammatory Drugs
Cell Biology
Frara N; Abdelmagid S M; Fisher P W; Popoff S N; Safadi F F; Barbe M F
Molecular Biology of the Cell
2014
2014-12
Journal Article or Conference Abstract Publication
n/a
Performance of Repetitive Tasks Induces Decreased Grip Strength and Increased Fibrogenic Proteins in Skeletal Muscle: Role of Force and Inflammation
carpal-tunnel-syndrome; factor gene-expression; flexor tendon cells; in-vivo; lengthening contractions; necrosis-factor-alpha; rat model; Science & Technology - Other Topics; strain injury; tissue growth-factor; tnf-alpha
Background: This study elucidates exposure-response relationships between performance of repetitive tasks, grip strength declines, and fibrogenic-related protein changes in muscles, and their link to inflammation. Specifically, we examined forearm flexor digitorum muscles for changes in connective tissue growth factor (CTGF; a matrix protein associated with fibrosis), collagen type I (Col1; a matrix component), and transforming growth factor beta 1 (TGFB1; an upstream modulator of CTGF and collagen), in rats performing one of two repetitive tasks, with or without anti-inflammatory drugs. Methodology/Results: To examine the roles of force versus repetition, rats performed either a high repetition negligible force food retrieval task (HRNF), or a high repetition high force handle-pulling task (HRHF), for up to 9 weeks, with results compared to trained only (TR-NF or TR-HF) and normal control rats. Grip strength declined with both tasks, with the greatest declines in 9-week HRHF rats. Quantitative PCR (qPCR) analyses of HRNF muscles showed increased expression of Col1 in weeks 3-9, and CTGF in weeks 6 and 9. Immunohistochemistry confirmed PCR results, and also showed greater increases of CTGF and collagen matrix in 9-week HRHF rats than 9-week HRNF rats. ELISA, and immunohistochemistry revealed greater increases of TGFB1 in TR-HF and 6-week HRHF, compared to 6-week HRNF rats. To examine the role of inflammation, results from 6-week HRHF rats were compared to rats receiving ibuprofen or anti-TNF-alpha treatment in HRHF weeks 4-6. Both treatments attenuated HRHF-induced increases in CTGF and fibrosis by 6 weeks of task performance. Ibuprofen attenuated TGFB1 increases and grip strength declines, matching our prior results with anti-TNF alpha. Conclusions/Significance: Performance of highly repetitive tasks was associated with force-dependent declines in grip strength and increased fibrogenic-related proteins in flexor digitorum muscles. These changes were attenuated, at least short-term, by anti-inflammatory treatments.
Abdelmagid S M; Barr A E; Rico M; Amin M; Litvin J; Popoff S N; Safadi F; Barbe M F
PLOS ONE
2012
2012-05
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1371/journal.pone.0038359" target="_blank" rel="noreferrer noopener">10.1371/journal.pone.0038359</a>