1
40
47
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0006-291x(88)80881-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0006-291x(88)80881-7</a>
Pages
576–580
Issue
1
Volume
156
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The complete nucleotide sequence of human liver cytochrome b5 mRNA.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1988
1988-10
Subject
The topic of the resource
Humans; Amino Acid Sequence; Liver/*metabolism; Base Sequence; Molecular Sequence Data; DNA/genetics; Cytochrome b Group/*genetics; Cytochromes b5; RNA; Cloning; Molecular; Messenger/*genetics
Creator
An entity primarily responsible for making the resource
Yoo M; Steggles A W
Description
An account of the resource
We have isolated and sequenced a cDNA clone corresponding to human liver cytochrome b5 mRNA. The 760 base pair (bp) sequence contains the complete coding and 3' non-translated regions plus 52 bp of 5' non-translated sequence. The derived amino acid sequence showed that the previous assignment of several amino acids was in error. In addition, the sequence of the previously unknown COOH hydrophobic region has been obtained.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0006-291x(88)80881-7" target="_blank" rel="noreferrer noopener">10.1016/s0006-291x(88)80881-7</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1988
Amino Acid Sequence
Base Sequence
Biochemical and biophysical research communications
Cloning
Cytochrome b Group/*genetics
Cytochromes b5
DNA/genetics
Humans
Liver/*metabolism
Messenger/*genetics
Molecular
Molecular Sequence Data
RNA
Steggles A W
Yoo M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0006-291x(89)92092-5" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0006-291x(89)92092-5</a>
Pages
18–24
Issue
1
Volume
163
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The characterization of three types of partially processed mRNA and two pseudogenes for human liver cytochrome b5.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1989
1989-08
Subject
The topic of the resource
Humans; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Liver/*physiology; DNA/genetics; Cytochrome b Group/*genetics; Cytochromes b5; Pseudogenes; Cloning; Molecular; Post-Transcriptional; RNA Processing
Creator
An entity primarily responsible for making the resource
Yoo M; Steggles A W
Description
An account of the resource
We have isolated cDNA clones corresponding to partially processed human liver cytochrome b5 mRNAs. All the clones contained poly(A) sequences, and one clone had a shorter 3' non-translated sequence, indicating the use of an alternative poly(A) addition signal. In addition, all the clones contained the coding information for amino acids 87-134; however, there were two types of intron junction adjacent to the coding sequence. Detailed analysis of the Type I clones showed that the Type II intron sequence was contained within the Type I sequence, but approximately 1000 bp 5' of the Type I intron-exon junction showed alternative splicing within this intron. In addition, we have isolated two pseudogenes which lack introns, suggesting the retroviral insertion of human liver cytochrome b5 mRNA sequences into the human genome.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0006-291x(89)92092-5" target="_blank" rel="noreferrer noopener">10.1016/0006-291x(89)92092-5</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1989
Amino Acid Sequence
Base Sequence
Biochemical and biophysical research communications
Cloning
Cytochrome b Group/*genetics
Cytochromes b5
DNA/genetics
Humans
Liver/*physiology
Molecular
Molecular Sequence Data
Post-Transcriptional
Pseudogenes
RNA Processing
Steggles A W
Yoo M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
63–73
Issue
1
Volume
1583
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
On the mechanism of bile acid inhibition of rat sterol 12alpha-hydroxylase gene (CYP8B1) transcription: roles of alpha-fetoprotein transcription factor and hepatocyte nuclear factor 4alpha.
Publisher
An entity responsible for making the resource available
Biochimica et biophysica acta
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-06
Subject
The topic of the resource
Animals; Rats; Gene Expression Regulation; Base Sequence; Cattle; Cytochrome P-450 Enzyme System/*genetics; Hepatocyte Nuclear Factor 4; *DNA-Binding Proteins; Bile Acids and Salts/*pharmacology; Steroid Hydroxylases/*genetics; alpha-Fetoproteins/genetics/*physiology; DNA; Phosphoproteins/genetics/*physiology; Steroid 12-alpha-Hydroxylase; Transcription Factors/genetics/*physiology; Sprague-Dawley; RNA; Transcription; Genetic; Promoter Regions; Messenger/genetics; Enzymologic/*drug effects/physiology; Genetic/*drug effects
Creator
An entity primarily responsible for making the resource
Yang Yizeng; Zhang Ming; Eggertsen Gosta; Chiang John Y L
Description
An account of the resource
The sterol 12alpha-hydroxylase (CYP8B1) is a key enzyme of the bile acid biosynthetic pathway. It regulates the composition of bile acids in bile, i.e. ratio between cholic acid (CA) and chenodeoxycholic acid (CDCA). In similarity with cholesterol 7alpha-hydroxylase (CYP7A1), this enzyme is subjected to a negative feedback regulation by bile acids. It has been recently reported that bile acid-activated farnesoid X receptor (FXR) induces the small heterodimer partner (SHP) that interacts with alpha-fetoprotein transcription factor (FTF) and down-regulates CYP7A1 transcription. We studied whether the same mechanism also regulated rat CYP8B1 gene transcription. Feeding rats with CDCA caused a
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA-Binding Proteins
2002
alpha-Fetoproteins/genetics/*physiology
Animals
Base Sequence
Bile Acids and Salts/*pharmacology
Biochimica et biophysica acta
Cattle
Chiang John Y L
Cytochrome P-450 Enzyme System/*genetics
Department of Integrative Medical Sciences
DNA
Eggertsen Gosta
Enzymologic/*drug effects/physiology
Gene Expression Regulation
Genetic
Genetic/*drug effects
Hepatocyte Nuclear Factor 4
Messenger/genetics
NEOMED College of Medicine
Phosphoproteins/genetics/*physiology
Promoter Regions
Rats
RNA
Sprague-Dawley
Steroid 12-alpha-Hydroxylase
Steroid Hydroxylases/*genetics
Transcription
Transcription Factors/genetics/*physiology
Yang Yizeng
Zhang Ming
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
191–197
Issue
1
Volume
272
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional regulation of human oxysterol 7 alpha-hydroxylase gene (CYP7B1) by Sp1.
Publisher
An entity responsible for making the resource available
Gene
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-07
Subject
The topic of the resource
Humans; Protein Binding; Gene Expression Regulation; Cell Line; Transfection; Base Sequence; Binding Sites/genetics; Molecular Sequence Data; Cytochrome P450 Family 7; Mutagenesis; Luciferases/genetics/metabolism; Recombinant Fusion Proteins/genetics/metabolism; CpG Islands/genetics; Cytochrome P-450 Enzyme System/*genetics/metabolism; DNA/genetics; Sequence Deletion; Sp1 Transcription Factor/metabolism/*physiology; Steroid Hydroxylases/*genetics/metabolism; Cultured; Binding; Competitive; Transcription; Genetic; Enzymologic; Tumor Cells; Site-Directed; Regulatory Sequences; Nucleic Acid/genetics
Creator
An entity primarily responsible for making the resource
Wu Z; Chiang J Y
Description
An account of the resource
Oxysterol 7 alpha-hydroxylase catalyzes hydroxylation of oxysterols and neurosterols and plays a role in the alternative bile acid synthesis pathway. This gene is widely expressed in many organs and peripheral tissues and may protect tissues from the toxicity of oxysterols. Mutation in CYP7B1 caused neonatal cholestasis. To examine the regulatory mechanisms governing CYP7B1 expression, the 5' flanking sequence of the CYP7B1 was analyzed and revealed a CpG island of about 1.2 kb. Transient transfection assays of deletion mutants of the CYP7B1 promoter-luciferase reporter gene in human liver-derived HepG2, fibroblast NT1088, and human embryonic kidney 293 cell lines revealed that the region from -291 to +189 was critical for gene transcription. Three GC box sequences located between -25 and +10 were essential for basal transcription because mutations of these sequences markedly reduced promoter activity. Sp1 and Sp3 bound to these sequences as demonstrated by DNase I footprinting assays and electrophoretic mobility shift assay. Thus, regulation of CYP7B1 transcription by Sp1 may play a pivotal role in regulating oxysterol levels, which regulate cholesterol metabolism.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2001
Base Sequence
Binding
Binding Sites/genetics
Cell Line
Chiang J Y
Competitive
CpG Islands/genetics
Cultured
Cytochrome P-450 Enzyme System/*genetics/metabolism
Cytochrome P450 Family 7
Department of Integrative Medical Sciences
DNA/genetics
Enzymologic
gene
Gene Expression Regulation
Genetic
Humans
Luciferases/genetics/metabolism
Molecular Sequence Data
Mutagenesis
NEOMED College of Medicine
Nucleic Acid/genetics
Protein Binding
Recombinant Fusion Proteins/genetics/metabolism
Regulatory Sequences
Sequence Deletion
Site-Directed
Sp1 Transcription Factor/metabolism/*physiology
Steroid Hydroxylases/*genetics/metabolism
Transcription
Transfection
Tumor Cells
Wu Z
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
2195–2203
Issue
12
Volume
40
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999-12
Subject
The topic of the resource
Humans; Animals; Mice; Cell Line; Transfection; Base Sequence; Molecular Sequence Data; Chromosome Mapping; Cytochrome P450 Family 7; DNA; Luciferases/genetics; Cytochrome P-450 Enzyme System/*genetics/metabolism; Steroid Hydroxylases/*genetics/metabolism; Hydroxycholesterols/metabolism; Codon; Northern; Blotting; Transcription; Genetic; Cloning; Molecular; Genetic/genetics; Promoter Regions; Nucleic Acid; Complementary/biosynthesis/*isolation & purification; Initiator; Regulatory Sequences
Creator
An entity primarily responsible for making the resource
Wu Z; Martin K O; Javitt N B; Chiang J Y
Description
An account of the resource
Oxysterol 7alpha-hydroxylase has broad substrate specificity for sterol metabolites and may be involved in many metabolic processes including bile acid synthesis and neurosteroid metabolism. The cloned human oxysterol 7alpha-hydroxylase (CYP7B1) cDNA encodes a polypeptide of 506 amino acid residues that shares 40% sequence identity to human cholesterol 7alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in the conversion of cholesterol to bile acids in the liver. In contrast to the liver-specific expression of CYP7A1, CYP7B1 mRNA transcripts were detected in human tissues involved in steroid genesis (brain, testes, ovary, and prostate) and in bile acid synthesis (liver) and reabsorption (colon, kidney, and small intestine). The human oxysterol 7alpha-hydroxylase transiently expressed in 293/T cells was able to catalyze 7alpha-hydroxylation of
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1999
Animals
Base Sequence
Blotting
Cell Line
Chiang J Y
Chromosome Mapping
Cloning
Codon
Complementary/biosynthesis/*isolation & purification
Cytochrome P-450 Enzyme System/*genetics/metabolism
Cytochrome P450 Family 7
Department of Integrative Medical Sciences
DNA
Genetic
Genetic/genetics
Humans
Hydroxycholesterols/metabolism
Initiator
Javitt N B
Journal of lipid research
Luciferases/genetics
Martin K O
Mice
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Northern
Nucleic Acid
Promoter Regions
Regulatory Sequences
Steroid Hydroxylases/*genetics/metabolism
Transcription
Transfection
Wu Z
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
1831–1841
Issue
9
Volume
37
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene (CYP7A) in HepG2 cells.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-09
Subject
The topic of the resource
Humans; Binding Sites; Gene Expression Regulation; Cell Line; Transfection; Base Sequence; Molecular Sequence Data; Phorbol Esters/pharmacology; DNA-Binding Proteins/genetics/metabolism; *Transcription Factors; Enzyme Repression; Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics; Consensus Sequence; Glucocorticoids/pharmacology; Hepatocyte Nuclear Factor 3-alpha; Insulin/pharmacology; Nuclear Proteins/genetics/metabolism; Recombinant Fusion Proteins/biosynthesis; Thyroid Hormones/pharmacology; Genes; Receptors; Enzymologic/*drug effects; Genetic; *Promoter Regions; Reporter; *Transcription; Glucocorticoid/genetics/metabolism
Creator
An entity primarily responsible for making the resource
Wang D P; Stroup D; Marrapodi M; Crestani M; Galli G; Chiang J Y
Description
An account of the resource
A stable HepG2 cell line harboring a human cholesterol 7 alpha-hydroxylase (CYP7A) minigene/luciferase reporter gene construct was selected for studying transcriptional regulation of CYP7A gene promoter. Insulin and phorbol
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
*Transcription
*Transcription Factors
1996
Base Sequence
Binding Sites
Cell Line
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics
Consensus Sequence
Crestani M
Department of Integrative Medical Sciences
DNA-Binding Proteins/genetics/metabolism
Enzyme Repression
Enzymologic/*drug effects
Galli G
Gene Expression Regulation
Genes
Genetic
Glucocorticoid/genetics/metabolism
Glucocorticoids/pharmacology
Hepatocyte Nuclear Factor 3-alpha
Humans
Insulin/pharmacology
Journal of lipid research
Marrapodi M
Molecular Sequence Data
NEOMED College of Medicine
Nuclear Proteins/genetics/metabolism
Phorbol Esters/pharmacology
Receptors
Recombinant Fusion Proteins/biosynthesis
Reporter
Stroup D
Thyroid Hormones/pharmacology
Transfection
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
1–11
Issue
1
Volume
41
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
HNF4 and COUP-TFII interact to modulate transcription of the cholesterol 7alpha-hydroxylase gene (CYP7A1).
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-01
Subject
The topic of the resource
Humans; Animals; Rats; Liver/metabolism; Recombinant Proteins/metabolism; Base Sequence; Mutation; DNA Primers; Cholesterol 7-alpha-Hydroxylase/*genetics; Transcription Factors/*metabolism; Hepatocyte Nuclear Factor 4; DNA-Binding Proteins/*metabolism; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Luciferases/genetics; COUP Transcription Factor II; COUP Transcription Factors; Phosphoproteins/*metabolism; Genes; Sprague-Dawley; Cultured; Genetic; Tumor Cells; Reporter; Promoter Regions; *Transcription; *Receptors; Steroid
Creator
An entity primarily responsible for making the resource
Stroup D; Chiang J Y
Description
An account of the resource
The gene for cholesterol 7alpha-hydroxylase (CYP7A1) contains a sequence at nt -149 to -118 that was found to play a large role in determining the overall transcriptional activity and regulation of the promoter. Hepatocyte nuclear factor 4 (HNF4) and chicken ovalbumin upstream promoter transcription factor II (COUP-TFII) synergistically activate transcription of the CYP7A1 promoter. Transactivation of CYP7A1 by HNF4 in the human hepatoma cell line, HepG2, was enhanced by cotransfection with COUP-TFII or the basal transcription element binding protein (BTEB). HNF4 prepared from rat liver nuclear extracts bound to oligomers homologous to the nt -146 to -134 sequences in electrophoretic mobility shift assays (EMSA), which corresponded to a conserved region containing a direct repeat of hormone response elements spaced by one nucleotide (DR1). The sequences surrounding this DR1 were found to be essential for the HNF4 transactivation. In vitro-translated COUP-TFII was found to bind the adjacent sequences from nt -139 to -128 (DR0), but COUP-TFII interacted with this region at a much lower affinity than to the COUP-TFII-site at nt -72 to -57 (DR4). Mutations at nt -139 to -128 or nt -72 to -57 reduced the COUP-TFII and HNF4 synergy; however, these
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Receptors
*Transcription
2000
Animals
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
COUP Transcription Factor II
COUP Transcription Factors
Cultured
Department of Integrative Medical Sciences
DNA Primers
DNA-Binding Proteins/*metabolism
Genes
Genetic
Hepatocyte Nuclear Factor 4
Humans
Journal of lipid research
Liver/metabolism
Luciferases/genetics
Mutation
NEOMED College of Medicine
Phosphoproteins/*metabolism
Promoter Regions
Rats
Recombinant Proteins/metabolism
Reporter
Sprague-Dawley
Steroid
Stroup D
Transcription Factors/*metabolism
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
12012–12019
Issue
20
Volume
265
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol 7 alpha-hydroxylase in the liver. Cloning, sequencing, and regulation of cholesterol 7 alpha-hydroxylase mRNA.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-07
Subject
The topic of the resource
Female; Animals; Rats; Amino Acid Sequence; *Gene Expression Regulation; Kinetics; Base Sequence; Immunoblotting; Molecular Sequence Data; Steroid Hydroxylases/*genetics; Circadian Rhythm; DNA/genetics/isolation & purification; Restriction Mapping; Enzyme Induction; Liver/drug effects/*enzymology; Cell Fractionation; Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics/immunology; Cholestyramine Resin/pharmacology; Cytochrome P-450 Enzyme System/genetics; Epitopes/analysis; Polyribosomes/metabolism/ultrastructure; Inbred Strains; RNA; Enzymologic; Sequence Homology; Cloning; Nucleic Acid; Messenger/*genetics; Centrifugation; Density Gradient; Molecular/methods
Creator
An entity primarily responsible for making the resource
Li Y C; Wang D P; Chiang J Y
Description
An account of the resource
Monospecific antibody against purified rat liver cholesterol 7 alpha-hydroxylase cytochrome P-450 was used to screen a lambda gt11 cDNA library constructed from immuno-enriched polysomal RNA of cholestyramine-treated female rat liver. Two types of cDNA clones differing in the length of the 3'-untranslated region were identified, and DNA sequences were determined. The full length clone contains 3561 base pairs plus a long poly(A) tail. The amino acid sequence deduced from the open reading frame revealed a unique P-450 protein containing 503 amino acid residues which belonged to a new gene family designated family VII or CYP7. Southern blot hybridization experiments indicated that the minimal size of P-450 VII gene was 11 kilobase pairs (kb), and there was probably only one gene in this new family. Northern blot hybridization using specific cDNA probes revealed at least two major mRNA species of about 4.0 kb and 2.1 kb, respectively. These two mRNA species may be derived from the use of different polyadenylation signals and reverse-transcribed to two types of cDNA clones. Cholesterol 7 alpha-hydroxylase mRNAs were induced 2- to 3-fold in rat liver by cholestyramine treatment. The mRNA level was rapidly reduced upon the removal of the inducer. Similarly, cholesterol feeding induced enzyme activity, protein, and mRNA levels in the rat by 2-fold, suggesting that cholesterol is an important regulator of cholesterol 7 alpha-hydroxylase in the liver. On the other hand, dexamethasone and pregnenolone-16 alpha-carbonitrile drastically reduced the activity, protein, and mRNA levels. These experiments suggest that the induction of cholesterol 7 alpha-hydroxylase activity by cholestyramine or cholesterol and inhibition of cholesterol 7 alpha-hydroxylase activity by bile acid feedback are results of the rapid turnover of cholesterol 7 alpha-hydroxylase enzyme and mRNA levels.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
1990
Amino Acid Sequence
Animals
Base Sequence
Cell Fractionation
Centrifugation
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics/immunology
Cholestyramine Resin/pharmacology
Circadian Rhythm
Cloning
Cytochrome P-450 Enzyme System/genetics
Density Gradient
Department of Integrative Medical Sciences
DNA/genetics/isolation & purification
Enzyme Induction
Enzymologic
Epitopes/analysis
Female
Immunoblotting
Inbred Strains
Kinetics
Li Y C
Liver/drug effects/*enzymology
Messenger/*genetics
Molecular Sequence Data
Molecular/methods
NEOMED College of Medicine
Nucleic Acid
Polyribosomes/metabolism/ultrastructure
Rats
Restriction Mapping
RNA
Sequence Homology
Steroid Hydroxylases/*genetics
The Journal of biological chemistry
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
19186–19191
Issue
29
Volume
266
Dublin Core
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Title
A name given to the resource
The expression of a catalytically active cholesterol 7 alpha-hydroxylase cytochrome P450 in Escherichia coli.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1991
1991-10
Subject
The topic of the resource
Gene Expression; Amino Acid Sequence; Base Sequence; Polymerase Chain Reaction; Liver/enzymology; Catalysis; Molecular Sequence Data; Substrate Specificity; Cholesterol/metabolism; Cholesterol 7-alpha-Hydroxylase/*genetics; DNA/genetics; Codon; Escherichia coli/*enzymology; Plasmids; Chromatography; Blotting; Western; Electrophoresis; Polyacrylamide Gel; Liquid; Microsomes
Creator
An entity primarily responsible for making the resource
Li Y C; Chiang J Y
Description
An account of the resource
We have recently cloned a full-length cDNA encoding the rat hepatic cholesterol 7 alpha-hydroxylase cytochrome P450 (P450c7) (Li, Y. C., Wang, D. P., and Chiang, J. Y. L. (1990) J. Biol. Chem. 265, 12012-12019), which catalyzes the rate-limiting reaction of bile acid synthesis in the liver. By using the polymerase chain reaction, we have designed two P450c7 cDNAs. One has the second Met codon deleted and the third Thr codon replaced with an Ala. The other lacks codons for the NH2-terminal hydrophobic sequence of amino acids 2-24 (P450c7 delta 2-24). The cDNAs were separately cloned into the expression vector pKK233-2 and transformed into Escherichia coli. After induction with isopropyl-beta-D-thiogalactopyranoside, bacteria harboring recombinant plasmids expressed a polypeptide which reacted with the antibody against cholesterol 7 alpha-hydroxylase in immunoblots. The slightly modified full-length enzyme was expressed to 0.2% of the total bacterial lysate and was located in the membrane fraction, whereas P450c7 delta 2-24 was expressed at a 10-fold higher level (2%), of which 85% was in the cytosol and the remaining associated with the membranes. We have purified P450c7 delta 2-24 which showed a typical reduced-CO difference spectrum of cytochrome P450 and reconstituted cholesterol 7 alpha-hydroxylase activity in the presence of NADPH-cytochrome P450 reductase. P450c7 delta 2-24 has a similar Km for cholesterol (24.6 microM) but a lower Vmax (0.10 nmol/min) and a lower turnover number (1.93 min-1) as compared with the enzyme isolated from rat liver microsomes. The purified P450c7 delta 2-24 has an unique hydrophilic NH2 terminus and contains monomers and dimers in equal amounts. This is the first report demonstrating that a genetically engineered cytochrome P450 enzyme lacking a typical NH2-terminal hydrophobic sequence is mainly cytosolic and catalytically active.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1991
Amino Acid Sequence
Base Sequence
Blotting
Catalysis
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cholesterol/metabolism
Chromatography
Codon
Department of Integrative Medical Sciences
DNA/genetics
Electrophoresis
Escherichia coli/*enzymology
Gene Expression
Li Y C
Liquid
Liver/enzymology
Microsomes
Molecular Sequence Data
NEOMED College of Medicine
Plasmids
Polyacrylamide Gel
Polymerase Chain Reaction
Substrate Specificity
The Journal of biological chemistry
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0003-9861(90)90664-k" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0003-9861(90)90664-k</a>
Pages
429–439
Issue
2
Volume
283
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cloning and characterization of two major 3-methylcholanthrene inducible hamster liver cytochrome P450s.
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-12
Subject
The topic of the resource
Female; Humans; Male; Animals; Rats; Amino Acid Sequence; Reference Values; Base Sequence; Liver/enzymology; Immunoblotting; Molecular Sequence Data; Cricetinae; Mesocricetus; Phenobarbital/pharmacology; Restriction Mapping; Nucleic Acid Hybridization; Cytochrome P-450 Enzyme System/biosynthesis/*genetics; Enzyme Induction; Liver/drug effects/*enzymology; Methylcholanthrene/*pharmacology; Inbred Strains; Sequence Homology; Cloning; Molecular; Nucleic Acid; Microsomes
Creator
An entity primarily responsible for making the resource
Lai T S; Chiang J Y
Description
An account of the resource
We have studied the immunochemical properties of two major 3-methylcholanthrene inducible hamster liver cytochrome P450 isozymes, P450 MC1 and P450 MC4. Immunoblots using specific antibodies against P450 MC1 and P450 MC4 demonstrated that these two P450s were present in very low levels in control hamster livers and were greatly induced by 3-methylcholanthrene treatment. P450 MC1 was immunochemically different from P450 MC4, rat P450c and P450d, and rabbit LM4. The immunorelated polypeptide to P450 MC1 was not present in the control or the
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0003-9861(90)90664-k" target="_blank" rel="noreferrer noopener">10.1016/0003-9861(90)90664-k</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1990
Amino Acid Sequence
Animals
Archives of biochemistry and biophysics
Base Sequence
Chiang J Y
Cloning
Cricetinae
Cytochrome P-450 Enzyme System/biosynthesis/*genetics
Department of Integrative Medical Sciences
Enzyme Induction
Female
Humans
Immunoblotting
Inbred Strains
Lai T S
Liver/drug effects/*enzymology
Liver/enzymology
Male
Mesocricetus
Methylcholanthrene/*pharmacology
Microsomes
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Nucleic Acid
Nucleic Acid Hybridization
Phenobarbital/pharmacology
Rats
Reference Values
Restriction Mapping
Sequence Homology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
588–595
Issue
2
Volume
185
Dublin Core
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Title
A name given to the resource
Polymorphisms of human cholesterol 7 alpha-hydroxylase.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
1992-06
Subject
The topic of the resource
Humans; Amino Acid Sequence; Base Sequence; Polymerase Chain Reaction; Molecular Sequence Data; Cholesterol 7-alpha-Hydroxylase/*genetics; DNA/genetics; Restriction Mapping; Oligodeoxyribonucleotides/chemistry; Genetic; Cloning; Molecular; Polymorphism
Creator
An entity primarily responsible for making the resource
Karam W G; Chiang J Y
Description
An account of the resource
Human liver cholesterol 7 alpha-hydroxylase (CYP7) cDNAs were isolated from a human liver cDNA library. A full-length cDNA has 2901 nucleotides which encode a typical P450 polypeptide of 504 amino acid residues. Two different sequences of codon 100, TTT (Phe) and TCT (Ser), were identified in cDNA clones. In addition, codons 347 and 385 are GAT (Asp) and GAC (Asp) in all cDNA clones, whereas those reported previously (FEBS Lett. 268, 137-140, 1990) are AAT (Asn) and AGC (Ser), respectively. Since there is only one 7 alpha-hydroxylase gene in the human genome, it is likely that polymorphisms at the codon 100 of cDNA clones arise from two different alleles in the 7 alpha-hydroxylase gene of this human liver.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1992
Amino Acid Sequence
Base Sequence
Biochemical and biophysical research communications
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cloning
Department of Integrative Medical Sciences
DNA/genetics
Genetic
Humans
Karam W G
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Oligodeoxyribonucleotides/chemistry
Polymerase Chain Reaction
Polymorphism
Restriction Mapping
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
1222–1231
Issue
7
Volume
35
Dublin Core
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Title
A name given to the resource
Expression and purification of human cholesterol 7 alpha-hydroxylase in Escherichia coli.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-07
Subject
The topic of the resource
Humans; Animals; Rats; Kinetics; Base Sequence; Catalysis; Molecular Sequence Data; Escherichia coli; Solubility; Cholesterol 7-alpha-Hydroxylase/biosynthesis/genetics/*isolation & purification; Cytochrome P-450 Enzyme System/biosynthesis/isolation & purification; Immunochemistry; Recombinant Proteins/biosynthesis; Subcellular Fractions/enzymology
Creator
An entity primarily responsible for making the resource
Karam W G; Chiang J Y
Description
An account of the resource
Cholesterol 7 alpha-hydroxylase (P450c7) is the first and rate-limiting enzyme in bile acid biosynthesis and is the product of a cytochrome P450 gene, CYP7. We have previously reported the cloning of a full-length human cholesterol 7 alpha-hydroxylase cDNA (Karam, W. G., and J. Y. L. Chiang. 1992. Biochem. Biophys. Res. Commun. 185: 588-595). Using this clone in a polymerase chain reaction, we have generated a cDNA (H7 alpha 1.5) in which the codons for the
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1994
Animals
Base Sequence
Catalysis
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/biosynthesis/genetics/*isolation & purification
Cytochrome P-450 Enzyme System/biosynthesis/isolation & purification
Department of Integrative Medical Sciences
Escherichia coli
Humans
Immunochemistry
Journal of lipid research
Karam W G
Kinetics
Molecular Sequence Data
NEOMED College of Medicine
Rats
Recombinant Proteins/biosynthesis
Solubility
Subcellular Fractions/enzymology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
13–17
Volume
5
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identification and nucleotide sequence of the leukocyte and reticulocyte forms of rabbit cytochrome b5 mRNA.
Publisher
An entity responsible for making the resource available
SAAS bulletin, biochemistry and biotechnology
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
1992-01
Subject
The topic of the resource
Animals; Amino Acid Sequence; Rabbits; Base Sequence; Polymerase Chain Reaction; Molecular Sequence Data; Nucleic Acid Hybridization; Cytochromes b5/chemistry/*genetics; Leukocytes/*chemistry; Liver/chemistry; Reticulocytes/*chemistry; RNA; Sequence Homology; Nucleic Acid; Messenger/*chemistry
Creator
An entity primarily responsible for making the resource
Giordano S J; Steggles A W
Description
An account of the resource
RNA extracted from rabbit leukocytes and reticulocytes was reverse transcribed and used in the Polymerase Chain Reaction technique along with primers designed to amplify the coding sequence of rabbit cytochrome b5. The resultant amplified products were subcloned and analyzed. Sequencing confirmed that leukocyte and liver cDNAs are homologous and encode the membrane-bound form of the protein. In contrast, reticulocytes exhibit a highly similar, but different mRNA which encodes the smaller, soluble cytochrome b5. This is the first example of a cytochrome b5 sequence from a tissue other than liver, erythrocyte or reticulocyte.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1992
Amino Acid Sequence
Animals
Base Sequence
Cytochromes b5/chemistry/*genetics
Giordano S J
Leukocytes/*chemistry
Liver/chemistry
Messenger/*chemistry
Molecular Sequence Data
Nucleic Acid
Nucleic Acid Hybridization
Polymerase Chain Reaction
Rabbits
Reticulocytes/*chemistry
RNA
SAAS bulletin, biochemistry and biotechnology
Sequence Homology
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
351–353
Issue
5
Volume
1
Dublin Core
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Title
A name given to the resource
The nucleotide sequence of rabbit liver cytochrome b5 mRNA.
Publisher
An entity responsible for making the resource available
Protein sequences & data analysis
Date
A point or period of time associated with an event in the lifecycle of the resource
1988
1905-06
Subject
The topic of the resource
Animals; Amino Acid Sequence; Liver/*metabolism; Rabbits; Base Sequence; Molecular Sequence Data; Cytochrome b Group/*genetics; Cytochromes b5; Chromosome Deletion; Genes; RNA; Cloning; Molecular; Messenger/*genetics
Creator
An entity primarily responsible for making the resource
Dariush N; Fisher C W; Steggles A W
Description
An account of the resource
Using a synthetic 26 base pair (bp) oligonucleotide, we have screened a rabbit liver cDNA library in lambda gt11 and isolated a 1000 bp DNA clone corresponding to cytochrome b5 mRNA. The clone contains the complete coding region and long 5' and 3' non-translated regions. The derived amino acid sequence (sequence; see text) agrees with published sequences, and confirms that the amino acids 62 and 104 are asparagine and aspartic acid, respectively.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1988
Amino Acid Sequence
Animals
Base Sequence
Chromosome Deletion
Cloning
Cytochrome b Group/*genetics
Cytochromes b5
Dariush N
Fisher C W
Genes
Liver/*metabolism
Messenger/*genetics
Molecular
Molecular Sequence Data
Protein sequences & data analysis
Rabbits
RNA
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
123–130; discussion 131
Volume
319
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Isolation and characterization of cDNA clones for human, bovine and rabbit liver cytochrome b5 mRNA's.
Publisher
An entity responsible for making the resource available
Progress in clinical and biological research
Date
A point or period of time associated with an event in the lifecycle of the resource
1989
1905-06
Subject
The topic of the resource
Humans; Animals; Amino Acid Sequence; Rabbits; Base Sequence; Molecular Sequence Data; Liver/*enzymology; Cattle; Chickens; Cytochromes b5/blood/*genetics; DNA/*isolation & purification; Erythrocytes/enzymology; RNA; Sequence Homology; Cloning; Molecular; Nucleic Acid; Messenger/*genetics
Creator
An entity primarily responsible for making the resource
Cristiano R J; Yoo M; Dariush N; Steggles A W
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1989
Amino Acid Sequence
Animals
Base Sequence
Cattle
Chickens
Cloning
Cristiano R J
Cytochromes b5/blood/*genetics
Dariush N
DNA/*isolation & purification
Erythrocytes/enzymology
Humans
Liver/*enzymology
Messenger/*genetics
Molecular
Molecular Sequence Data
Nucleic Acid
Progress in clinical and biological research
Rabbits
RNA
Sequence Homology
Steggles A W
Yoo M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1093/nar/17.2.799" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/nar/17.2.799</a>
Pages
799–799
Issue
2
Volume
17
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The complete nucleotide sequence of bovine liver cytochrome b5 mRNA.
Publisher
An entity responsible for making the resource available
Nucleic acids research
Date
A point or period of time associated with an event in the lifecycle of the resource
1989
1989-01
Subject
The topic of the resource
Animals; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Liver/*enzymology; Cattle; Cytochromes b5; Cytochrome b Group/*genetics/isolation & purification; RNA; Messenger/*isolation & purification
Creator
An entity primarily responsible for making the resource
Cristiano R J; Steggles A W
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1093/nar/17.2.799" target="_blank" rel="noreferrer noopener">10.1093/nar/17.2.799</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1989
Amino Acid Sequence
Animals
Base Sequence
Cattle
Cristiano R J
Cytochrome b Group/*genetics/isolation & purification
Cytochromes b5
Liver/*enzymology
Messenger/*isolation & purification
Molecular Sequence Data
Nucleic acids research
RNA
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
2192–2200
Issue
11
Volume
39
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional activation of the cholesterol 7alpha-hydroxylase gene (CYP7A) by nuclear hormone receptors.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1998
1998-11
Subject
The topic of the resource
Animals; Rats; Transcription Factors/metabolism; Base Sequence; Molecular Sequence Data; DNA/metabolism; Cholesterol 7-alpha-Hydroxylase/*genetics; Hepatocyte Nuclear Factor 4; Mutagenesis; Luciferases/genetics; Retinoid X Receptors; Phosphoproteins/metabolism; COUP Transcription Factor II; COUP Transcription Factors; *Transcriptional Activation; Bile Acids and Salts/biosynthesis; DNA-Binding Proteins/metabolism; Hormones/*physiology; Oligonucleotide Probes/metabolism; Genes; Cultured; Receptors; Genetic; Cytoplasmic and Nuclear/*physiology; Tumor Cells; Reporter; Retinoic Acid/metabolism; Promoter Regions; Nucleic Acid; Site-Directed; *Receptors; Repetitive Sequences; Steroid
Creator
An entity primarily responsible for making the resource
Crestani M; Sadeghpour A; Stroup D; Galli G; Chiang J Y
Description
An account of the resource
The gene encoding cholesterol 7alpha-hydroxylase (CYP7A), the rate-limiting enzyme in bile acid synthesis, is transcriptionally regulated by bile acids and hormones. Previously, we have identified two bile acid response elements (BARE) in the promoter of the CYP7A gene. The BARE II is located in nt -149/-118 region and contains three hormone response element (HRE)-like sequences that form two overlapping nuclear receptor binding sites. One is a direct repeat separated by one nucleotide DR1 (-146- TGGACTtAGTTCA-134) and the other is a direct repeat separated by five nucleotides DR5 (-139-AGTTCAaggccGGG TAA-123). Mutagenesis of these HRE sequences resulted in lower transcriptional activity of the CYP7A promoter/reporter genes in transient transfection assay in HepG2 cells. The orphan nuclear receptor, hepatocyte nuclear factor 4 (HNF-4)1, binds to the DR1 sequence as assessed by electrophoretic mobility shift assay, and activates the CYP7A promoter/reporter activity by about 9-fold. Cotransfection of HNF-4 plasmid with another orphan nuclear receptor, chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII), synergistically activated the CYP7A transcription by 80-fold. The DR5 binds the RXR/RAR heterodimer. A hepatocyte nuclear factor-3 (HNF-3) binding site (-175-TGTTTGTTCT-166) was identified. HNF-3 was required for both basal transcriptional activity and stimulation of the rat CYP7A promoter activity by retinoic acid. Combinatorial interactions and binding of these transcription factors to BAREs may modulate the promoter activity and also mediate bile acid repression of CYP7A gene transcription.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Receptors
*Transcriptional Activation
1998
Animals
Base Sequence
Bile Acids and Salts/biosynthesis
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
COUP Transcription Factor II
COUP Transcription Factors
Crestani M
Cultured
Cytoplasmic and Nuclear/*physiology
Department of Integrative Medical Sciences
DNA-Binding Proteins/metabolism
DNA/metabolism
Galli G
Genes
Genetic
Hepatocyte Nuclear Factor 4
Hormones/*physiology
Journal of lipid research
Luciferases/genetics
Molecular Sequence Data
Mutagenesis
NEOMED College of Medicine
Nucleic Acid
Oligonucleotide Probes/metabolism
Phosphoproteins/metabolism
Promoter Regions
Rats
Receptors
Repetitive Sequences
Reporter
Retinoic Acid/metabolism
Retinoid X Receptors
Sadeghpour A
Site-Directed
Steroid
Stroup D
Transcription Factors/metabolism
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
2419–2432
Issue
11
Volume
36
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Hormonal regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7).
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-11
Subject
The topic of the resource
Animals; Rats; Gene Expression Regulation; Cell Count; Transfection; Base Sequence; Molecular Sequence Data; Phorbol Esters/pharmacology; Cholesterol 7-alpha-Hydroxylase/*genetics; Luciferases/genetics; Cyclic AMP/pharmacology; Hormones/*pharmacology; Second Messenger Systems/physiology; Cultured; Genetic; Tumor Cells; Cloning; Molecular; *Promoter Regions; *Transcription; Enzymologic/*physiology
Creator
An entity primarily responsible for making the resource
Crestani M; Stroup D; Chiang J Y
Description
An account of the resource
The transcriptional regulation of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) by hormones and signal transduction pathways was studied by transient transfection assay of the promoter activity. HepG2 cells were transfected with deletion mutants of the CYP7 upstream region linked to the luciferase reporter gene. The transcription of CYP7/luciferase chimeric genes was higher in confluent than in subconfluent cultures of HepG2 cells. Glucocorticoid receptors, in the presence of dexamethasone, up-regulated the CYP7 gene through two regions located between -3262 and -2803, and between -344 and -222, respectively. Thyroid hormones did not have any effect on the promoter activity. Insulin inhibited the promoter activity through sequences located between -344 and -222, and abolished the stimulation by dexamethasone. Hence, the insulin effect was dominant over that of glucocorticoids. Treatment of transfected HepG2 cells with phorbol
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
*Transcription
1995
Animals
Base Sequence
Cell Count
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cloning
Crestani M
Cultured
Cyclic AMP/pharmacology
Department of Integrative Medical Sciences
Enzymologic/*physiology
Gene Expression Regulation
Genetic
Hormones/*pharmacology
Journal of lipid research
Luciferases/genetics
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Phorbol Esters/pharmacology
Rats
Second Messenger Systems/physiology
Stroup D
Transfection
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
17502–17507
Issue
26
Volume
269
Dublin Core
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Title
A name given to the resource
Identification and characterization of a putative bile acid-responsive element in cholesterol 7 alpha-hydroxylase gene promoter.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-07
Subject
The topic of the resource
Humans; Animals; Rats; Gene Expression Regulation; Base Sequence; Bile Acids and Salts/*metabolism; Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism; Molecular Sequence Data; Recombinant Fusion Proteins/genetics/metabolism; Luciferases/genetics; DNA-Binding Proteins/metabolism; Deoxyribonuclease I; Nuclear Proteins/metabolism; Oligodeoxyribonucleotides; Thyroid Hormones/genetics; Sprague-Dawley; Genetic; Enzymologic; Cloning; Molecular; *Promoter Regions; Antigens; Polyomavirus Transforming/genetics
Creator
An entity primarily responsible for making the resource
Chiang J Y; Stroup D
Description
An account of the resource
Nucleotide sequences of a 7997-base pair SacI fragment spanning 3643 base pairs of the upstream promoter region to exon 4 of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) have been determined. DNase I footprinting and electrophoretic mobility shift assay of the proximal promoter from nucleotides -346 to +36 revealed two protected regions which specifically shifted proteins in rat liver nuclear extracts. Footprint A (nucleotides -81 to -35) contained a cluster of overlapping sequence motifs of TGT3, steroid/thyroid hormone response elements (7 alpha TRE), hepatocyte nuclear factors 1 and 4, and CAAT/enhancer-binding protein alpha and has been shown to confer bile acid repression of the CYP7 gene promoter activity. Footprint B (nucleotides -148 to -129) contained a sequence motif HNF4. When footprint A (-101 to -49) or 7 alpha TRE (-73 to -55) sequence was linked upstream to a heterologous SV40 promoter/luciferase plasmid and transiently transfected into HepG2 cells, taurodeoxycholate suppressed the SV40 promoter activity. Electrophoretic mobility shift assays revealed that one or two bands shifted by the 7 alpha TRE or by a direct repeat sequence in 7 alpha TRE were absent when liver nuclear extracts of deoxycholic acid-treated rats were used. Similar gel shift patterns were also observed when human 7 alpha TRE or human liver nuclear extracts were used. The rat direct repeat sequence interacted with two polypeptides (M(r) = 57,000 and 116,000) in both rat and human liver nuclear extracts. These results suggest that hydrophobic bile acids may suppress the CYP7 gene expression by binding to a bile acid receptor which interacts with and prevents the binding of liver nuclear protein(s) to a bile acid-responsive element and that the core of bile acid-responsive element is a direct repeat.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
1994
Animals
Antigens
Base Sequence
Bile Acids and Salts/*metabolism
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism
Cloning
Deoxyribonuclease I
Department of Integrative Medical Sciences
DNA-Binding Proteins/metabolism
Enzymologic
Gene Expression Regulation
Genetic
Humans
Luciferases/genetics
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Nuclear Proteins/metabolism
Oligodeoxyribonucleotides
Polyomavirus Transforming/genetics
Rats
Recombinant Fusion Proteins/genetics/metabolism
Sprague-Dawley
Stroup D
The Journal of biological chemistry
Thyroid Hormones/genetics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
337–339
Issue
3
Volume
1132
Dublin Core
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Title
A name given to the resource
Cloning and 5'-flanking sequence of a rat cholesterol 7 alpha-hydroxylase gene.
Publisher
An entity responsible for making the resource available
Biochimica et biophysica acta
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
1992-10
Subject
The topic of the resource
Animals; Rats; Amino Acid Sequence; Base Sequence; Molecular Sequence Data; Cholesterol 7-alpha-Hydroxylase/*genetics; DNA; Restriction Mapping; Genetic; Cloning; Molecular; *Promoter Regions
Creator
An entity primarily responsible for making the resource
Chiang J Y; Yang T P; Wang D P
Description
An account of the resource
A cholesterol 7 alpha-hydroxylase gene containing 8 kb of the 5'-flanking region and 5 kb of the transcription region which covers exons 1 to 5 was isolated from a rat genomic library. The 2015 bp nucleotide sequence 5'-upstream from the start codon was determined. This promotor region contains many liver-enriched or -specific elements (TGT3, HNF/LF-B1), putative hormone responsive elements (TRE, GRE, RRE or RARE) and ubiquitous transcription factor binding sites (NF-1,
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
1992
Amino Acid Sequence
Animals
Base Sequence
Biochimica et biophysica acta
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cloning
Department of Integrative Medical Sciences
DNA
Genetic
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Rats
Restriction Mapping
Wang D P
Yang T P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
71–82
Volume
313
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of human sterol 27-hydroxylase gene (CYP27A1) by bile acids and hepatocyte nuclear factor 4alpha (HNF4alpha).
Publisher
An entity responsible for making the resource available
Gene
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-08
Subject
The topic of the resource
Humans; Cell Line; Transfection; Gene Expression Regulation/drug effects; Base Sequence; Binding Sites/genetics; Response Elements/genetics; Molecular Sequence Data; Mutation; Chenodeoxycholic Acid/pharmacology; Transcription Factors/genetics/*metabolism; Hepatocyte Nuclear Factor 4; Mutagenesis; *DNA-Binding Proteins; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Bile Acids and Salts/*pharmacology; Cholestanetriol 26-Monooxygenase; DNA/chemistry/genetics; Luciferases/genetics/metabolism; Phosphoproteins/genetics/*metabolism; Recombinant Fusion Proteins/genetics/metabolism; Steroid Hydroxylases/*genetics; DNA; Dose-Response Relationship; Drug; Cultured; Receptors; Tumor Cells; Cloning; Molecular; Sequence Analysis; Promoter Regions; Genetic/*genetics; Cytoplasmic and Nuclear/genetics/metabolism; Site-Directed
Creator
An entity primarily responsible for making the resource
Chen Wenling; Chiang John Y L
Description
An account of the resource
Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyses sterol side-chain oxidation of bile acid synthesis from cholesterol, and the first reaction of the acidic bile acid biosynthetic pathway. Hydrophobic bile acids suppress human CYP27A1 gene reporter activity when assayed in human hepatocellular blastoma HepG2 cells. Bile acids also inhibit CYP27A1 reporter activity in human embryonic kidney 293 cells. A putative bile acid response element (BARE) was mapped to a region downstream of nt -147 of the human CYP27A1 gene, within which a binding site for a liver-specific nuclear receptor, HNF4alpha, is identified. HNF4alpha strongly stimulates CYP27A1 gene transcription and mutation of its binding site markedly reduced promoter activity. Results suggest that human CYP27A1 gene transcription is suppressed by bile acids and HNF4alpha plays a pivotal role in transcriptional regulation of this gene.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA-Binding Proteins
2003
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Bile Acids and Salts/*pharmacology
Binding Sites/genetics
Cell Line
Chen Wenling
Chenodeoxycholic Acid/pharmacology
Chiang John Y L
Cholestanetriol 26-Monooxygenase
Cloning
Cultured
Cytoplasmic and Nuclear/genetics/metabolism
Department of Integrative Medical Sciences
DNA
DNA/chemistry/genetics
Dose-Response Relationship
Drug
gene
Gene Expression Regulation/drug effects
Genetic/*genetics
Hepatocyte Nuclear Factor 4
Humans
Luciferases/genetics/metabolism
Molecular
Molecular Sequence Data
Mutagenesis
Mutation
NEOMED College of Medicine
Phosphoproteins/genetics/*metabolism
Promoter Regions
Receptors
Recombinant Fusion Proteins/genetics/metabolism
Response Elements/genetics
Sequence Analysis
Site-Directed
Steroid Hydroxylases/*genetics
Transcription Factors/genetics/*metabolism
Transfection
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
3843–3853
Issue
5
Volume
73
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Intrastrain variants of herpes simplex virus type 1 isolated from a neonate with fatal disseminated infection differ in the ICP34.5 gene, glycoprotein processing, and neuroinvasiveness.
Publisher
An entity responsible for making the resource available
Journal of virology
Date
A point or period of time associated with an event in the lifecycle of the resource
1999
1999-05
Subject
The topic of the resource
Humans; Male; Animals; Mice; Amino Acid Sequence; *Genetic Variation; Base Sequence; Molecular Sequence Data; Polymerase Chain Reaction/methods; DNA; Deoxyribonuclease BamHI; Deoxyribonuclease EcoRI; Glycoproteins/metabolism; Viral Envelope Proteins/analysis; Viral Proteins/*genetics; Genes; Viral; Animal; Disease Models; Herpesvirus 1; Inbred BALB C; Amino Acid; Sequence Homology; Sequence Analysis; Nucleic Acid; Polymorphism; *Protein Processing; Post-Translational; Human/*genetics/growth & development/isolation & purification; Restriction Fragment Length
Creator
An entity primarily responsible for making the resource
Bower J R; Mao H; Durishin C; Rozenbom E; Detwiler M; Rempinski D; Karban T L; Rosenthal K S
Description
An account of the resource
Two intrastrain variants of herpes simplex virus type 1 (HSV-1) were isolated from a newborn with fatal disseminated infection. A small-plaque-producing variant (SP7) was the predominant virus (\textgreater99%) in the brain, and a large-plaque-producing variant (LP5) was the predominant virus (\textgreater99%) in the lung and gastrointestinal tract. EcoRI and BamHI restriction fragment patterns indicated that SP7 and LP5 are related strains. The large-plaque variants produced plaques similar in size to those produced by HSV-1 KOS. Unlike LP5 or KOS, SP7 was highly cell associated and processing of glycoprotein C and glycoprotein D was limited to precursor forms in infected Vero cells. The large-plaque phenotype from KOS could be transferred into SP7 by cotransfection of plasmids containing the EK or JK EcoRI fragment or a 3-kb plasmid with the UL34.5 gene of HSV-1 KOS together with SP7 DNA. PCR analysis using primers from within the ICP34.5 gene indicated differences for SP7, LP5, and KOS. Sequencing data indicated two sets of deletions in the UL34.5 gene that distinguish SP7 from LP5. Both SP7 and LP5 variants were neurovirulent (lethal following intracranial inoculation of young BALB/c mice); however, the LP5 variant was much less able to cause lethal neuroinvasive disease (footpad inoculation) whereas KOS caused no disease. Passage of SP7 selected for viruses (SLP-5 and SLP-10) which were attenuated for lethal neuroinvasive disease, were not cell-associated, and differed in the UL34.5 gene. UL34.5 from SLP-5 or SLP-10 resembled that of KOS. These findings support a role for UL34.5 in promoting virus egress and for neuroinvasive disease.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Genetic Variation
*Protein Processing
1999
Amino Acid
Amino Acid Sequence
Animal
Animals
Base Sequence
Bower J R
Deoxyribonuclease BamHI
Deoxyribonuclease EcoRI
Detwiler M
Disease Models
DNA
Durishin C
Genes
Glycoproteins/metabolism
Herpesvirus 1
Human/*genetics/growth & development/isolation & purification
Humans
Inbred BALB C
Journal of virology
Karban T L
Male
Mao H
Mice
Molecular Sequence Data
Nucleic Acid
Polymerase Chain Reaction/methods
Polymorphism
Post-Translational
Rempinski D
Restriction Fragment Length
Rosenthal K S
Rozenbom E
Sequence Analysis
Sequence Homology
Viral
Viral Envelope Proteins/analysis
Viral Proteins/*genetics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1194/jlr.M600282-JLR200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1194/jlr.M600282-JLR200</a>
Pages
373–384
Issue
2
Volume
48
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
PXR induces CYP27A1 and regulates cholesterol metabolism in the intestine.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007-02
Subject
The topic of the resource
*Lipid Metabolism; ATP Binding Cassette Transporter; ATP Binding Cassette Transporter 1; ATP-Binding Cassette Transporters/genetics; Base Sequence; Cell Line; Cholestanetriol 26-Monooxygenase/*metabolism; Cholesterol; Cholesterol/*metabolism; Fluorinated; Genes; Genetic/drug effects; Genetic/genetics; HDL/metabolism; Hepatocytes/drug effects/enzymology/metabolism; Humans; Hydrocarbons; Hydroxycholesterols/metabolism/pharmacology; Intestinal Mucosa/metabolism; Intestines/cytology/drug effects/enzymology; Member 1; Messenger/genetics/metabolism; Molecular Sequence Data; Pregnane X Receptor; Promoter Regions; Receptors; Reporter; Response Elements/genetics; Rifampin/pharmacology; RNA; Steroid/*metabolism; Subfamily G; Sulfonamides/pharmacology; Transcription; Up-Regulation/drug effects
Creator
An entity primarily responsible for making the resource
Li Tiangang; Chen Wenling; Chiang John Y L
Description
An account of the resource
Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1194/jlr.M600282-JLR200" target="_blank" rel="noreferrer noopener">10.1194/jlr.M600282-JLR200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Lipid Metabolism
2007
ATP Binding Cassette Transporter
ATP Binding Cassette Transporter 1
ATP-Binding Cassette Transporters/genetics
Base Sequence
Cell Line
Chen Wenling
Chiang John Y L
Cholestanetriol 26-Monooxygenase/*metabolism
Cholesterol
Cholesterol/*metabolism
Department of Integrative Medical Sciences
Fluorinated
Genes
Genetic/drug effects
Genetic/genetics
HDL/metabolism
Hepatocytes/drug effects/enzymology/metabolism
Humans
Hydrocarbons
Hydroxycholesterols/metabolism/pharmacology
Intestinal Mucosa/metabolism
Intestines/cytology/drug effects/enzymology
Journal of lipid research
Li Tiangang
Member 1
Messenger/genetics/metabolism
Molecular Sequence Data
NEOMED College of Medicine
Pregnane X Receptor
Promoter Regions
Receptors
Reporter
Response Elements/genetics
Rifampin/pharmacology
RNA
Steroid/*metabolism
Subfamily G
Sulfonamides/pharmacology
Transcription
Up-Regulation/drug effects
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1194/jlr.M004531" target="_blank" rel="noreferrer noopener">http://doi.org/10.1194/jlr.M004531</a>
Pages
2223–2233
Issue
8
Volume
51
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A putative role of micro RNA in regulation of cholesterol 7alpha-hydroxylase expression in human hepatocytes.
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
2010
2010-08
Subject
The topic of the resource
3' Untranslated Regions/genetics; Base Sequence; Chenodeoxycholic Acid/pharmacology; Cholesterol 7-alpha-Hydroxylase/*genetics; Enzymologic/drug effects/*genetics; Fibroblast Growth Factors/pharmacology; Gene Expression Regulation; Genetic/drug effects/genetics; Hep G2 Cells; Hepatocytes/drug effects/enzymology/*metabolism; Humans; Isoxazoles/pharmacology; MicroRNAs/*genetics/*metabolism; Oligonucleotide Array Sequence Analysis; Post-Transcriptional/drug effects/genetics; RNA Processing; Transcription
Creator
An entity primarily responsible for making the resource
Song Kwang-Hoon; Li Tiangang; Owsley Erika; Chiang John Y L
Description
An account of the resource
Cholesterol 7alpha-hydroxylase (CYP7A1) plays a critical role in regulation of bile acid synthesis in the liver. CYP7A1 mRNAs have very short half-lives, and bile acids destabilize CYP7A1 mRNA via the 3'-untranslated region (3'-UTR). However, the underlying mechanism of translational regulation of CYP7A1 mRNA remains unknown. Screening of a human micro RNA (miRNA) microarray has identified five differentially expressed miRNAs in human primary hepatocytes treated with chenodeoxycholic acid, GW4064, or fibroblast growth factor (FGF)19. These compounds also significantly induced the expression of miR-122a, a liver-specific and the predominant miRNA in human hepatocytes. The putative recognition sequences for miR-122a and miR-422a were localized in the 3'-UTR of human CYP7A1 mRNA. The miR-122a and miR-422a mimics inhibited, whereas their inhibitors stimulated CYP7A1 mRNA expression. These miRNAs specifically inhibited the activity of the CYP7A1-3'-UTR reporter plasmids, and mutations of miRNA binding sites in 3'-UTR abrogated miRNA inhibition of reporter activity. These results suggest that miR-122a and miR-422a may destabilize CYP7A1 mRNA to inhibit CYP7A1 expression. However, these miRNAs did not play a role in mediating FGF19 inhibition of CYP7A1 transcription. Under certain conditions, miRNA may reduce CYP7A1 mRNA stability to inhibit bile acid synthesis, and the miR-122a antagomirs may stimulate bile acid synthesis to reduce serum cholesterol and triglycerides.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1194/jlr.M004531" target="_blank" rel="noreferrer noopener">10.1194/jlr.M004531</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2010
3' Untranslated Regions/genetics
Base Sequence
Chenodeoxycholic Acid/pharmacology
Chiang John Y L
Cholesterol 7-alpha-Hydroxylase/*genetics
Department of Integrative Medical Sciences
Enzymologic/drug effects/*genetics
Fibroblast Growth Factors/pharmacology
Gene Expression Regulation
Genetic/drug effects/genetics
Hep G2 Cells
Hepatocytes/drug effects/enzymology/*metabolism
Humans
Isoxazoles/pharmacology
Journal of lipid research
Li Tiangang
MicroRNAs/*genetics/*metabolism
NEOMED College of Medicine
Oligonucleotide Array Sequence Analysis
Owsley Erika
Post-Transcriptional/drug effects/genetics
RNA Processing
Song Kwang-Hoon
Transcription
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajplung.90506.2008" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajplung.90506.2008</a>
Pages
L527–533
Issue
3
Volume
296
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
IL-1beta-induced cortisol stimulates lung fluid absorption in fetal guinea pigs via SGK-mediated Nedd4-2 inhibition.
Publisher
An entity responsible for making the resource available
American journal of physiology. Lung cellular and molecular physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-03
Subject
The topic of the resource
Absorption/drug effects; Animals; Base Sequence; Body Fluids/metabolism; DNA/genetics; Endosomal Sorting Complexes Required for Transport; Epithelial Sodium Channels/metabolism; Female; Guinea Pigs; Hydrocortisone/*biosynthesis/blood/physiology; Immediate-Early Proteins/genetics/*metabolism; Interleukin-1beta/*pharmacology; Lung/*drug effects/*physiology; Molecular Sequence Data; Nedd4 Ubiquitin Protein Ligases; Pregnancy; Protein-Serine-Threonine Kinases/genetics/*metabolism; Recombinant Proteins/pharmacology; Ubiquitin-Protein Ligases/*antagonists & inhibitors/genetics
Creator
An entity primarily responsible for making the resource
Li Tianbo; Koshy Shyny; Folkesson Hans G
Description
An account of the resource
We tested the hypothesis that interleukin (IL)-1beta-induced cortisol synthesis stimulates distal lung fluid absorption in fetal guinea pigs via induction of serum- and glucocorticoid-regulated kinase (SGK) and inhibition of neural precursor cell expressed, developmentally downregulated protein 4-2 (Nedd4-2).
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajplung.90506.2008" target="_blank" rel="noreferrer noopener">10.1152/ajplung.90506.2008</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
Absorption/drug effects
American journal of physiology. Lung cellular and molecular physiology
Animals
Base Sequence
Body Fluids/metabolism
DNA/genetics
Endosomal Sorting Complexes Required for Transport
Epithelial Sodium Channels/metabolism
Female
Folkesson Hans G
Guinea Pigs
Hydrocortisone/*biosynthesis/blood/physiology
Immediate-Early Proteins/genetics/*metabolism
Interleukin-1beta/*pharmacology
Koshy Shyny
Li Tianbo
Lung/*drug effects/*physiology
Molecular Sequence Data
Nedd4 Ubiquitin Protein Ligases
Pregnancy
Protein-Serine-Threonine Kinases/genetics/*metabolism
Recombinant Proteins/pharmacology
Ubiquitin-Protein Ligases/*antagonists & inhibitors/genetics
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajpgi.1997.273.2.G508" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpgi.1997.273.2.G508</a>
Pages
G508–517
Issue
2
Volume
273
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Identification of a bile acid response element in the cholesterol 7 alpha-hydroxylase gene CYP7A.
Publisher
An entity responsible for making the resource available
The American journal of physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-08
Subject
The topic of the resource
Animals; Base Sequence; Bile Acids and Salts/*pharmacology; Cholesterol 7-alpha-Hydroxylase/*genetics; Cultured; DNA-Binding Proteins/metabolism; Feedback; Genes; Genes/*drug effects; Luciferases/genetics; Rats; Reporter; Tumor Cells
Creator
An entity primarily responsible for making the resource
Stroup D; Crestani M; Chiang J Y
Description
An account of the resource
The transcriptional activity of the cholesterol 7 alpha-hydroxylase gene CYP7A is repressed by bile acids. Taurine conjugates of chenodeoxycholate and deoxycholate, but not cholate and ursodeoxycholate, inhibited the CYP7A promoter/luciferase reporter activity in transient transfection assays in Hep G2 cells. A region from nucleotide (nt) -74 to -55 was found to mediate bile acid response. However, deletion of this bile acid response element (BARE-I) enhanced reporter activity but did not eliminate the bile acid response. This is due to the presence of another BARE-II located in a conserved region between nt -149 and -128. Deletion or mutations of these sequences reduced promoter activity and abolished bile acid repression. This BARE-II shares an identical AGTTCAAG core sequence with BARE-I. Electrophoretic mobility shift assays of BARE-I and BARE-II probes using Hep G2 nuclear extract and the partially purified binding activity of nt -65/-54 DNA-affinity column revealed that the same or a similar nuclear protein might bind to both BAREs. BARE-II is the major BARE involved in the transcriptional repression of the CYP7A gene by hydrophobic bile acids.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajpgi.1997.273.2.G508" target="_blank" rel="noreferrer noopener">10.1152/ajpgi.1997.273.2.G508</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1997
Animals
Base Sequence
Bile Acids and Salts/*pharmacology
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Crestani M
Cultured
Department of Integrative Medical Sciences
DNA-Binding Proteins/metabolism
Feedback
Genes
Genes/*drug effects
Luciferases/genetics
NEOMED College of Medicine
Rats
Reporter
Stroup D
The American journal of physiology
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1124/dmd.108.025155" target="_blank" rel="noreferrer noopener">http://doi.org/10.1124/dmd.108.025155</a>
Pages
469–478
Issue
3
Volume
37
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Mechanism of vitamin D receptor inhibition of cholesterol 7alpha-hydroxylase gene transcription in human hepatocytes.
Publisher
An entity responsible for making the resource available
Drug metabolism and disposition: the biological fate of chemicals
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-03
Subject
The topic of the resource
Base Sequence; Calcitriol/drug effects/genetics/*physiology; Cell Line; Cells; Cholesterol 7-alpha-Hydroxylase/*genetics; Cultured; DNA Primers; Electrophoretic Mobility Shift Assay; Gene Knockdown Techniques; Genetic/*physiology; Hepatocytes/*drug effects/enzymology; Humans; Immunoprecipitation; Lithocholic Acid/pharmacology; Messenger/genetics; Polymerase Chain Reaction; Receptors; RNA; Small Interfering; Transcription; Tumor; Two-Hybrid System Techniques
Creator
An entity primarily responsible for making the resource
Han Shuxin; Chiang John Y L
Description
An account of the resource
Lithocholic acid (LCA) is a potent endogenous vitamin D receptor (VDR) ligand. In cholestasis, LCA levels increase in the liver and intestine. The objective of this study is to test the hypothesis that VDR plays a role in inhibiting cholesterol 7alpha-hydroxylase (CYP7A1) gene expression and bile acid synthesis in human hepatocytes. Immunoblot analysis has detected VDR proteins in the nucleus of the human hepatoma cell line HepG2 and human primary hepatocytes. 1alpha, 25-Dihydroxy-vitamin D(3) or LCA acetate-activated VDR inhibited CYP7A1 mRNA expression and bile acid synthesis, whereas small interfering RNA to VDR completely abrogated VDR inhibition of CYP7A1 mRNA expression in HepG2 cells. Electrophoretic mobility shift assay and mutagenesis analyses have identified the negative VDR response elements that bind VDR/retinoid X receptor alpha in the human CYP7A1 promoter. Mammalian two-hybrid, coimmunoprecipitation, glutathione
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1124/dmd.108.025155" target="_blank" rel="noreferrer noopener">10.1124/dmd.108.025155</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
Base Sequence
Calcitriol/drug effects/genetics/*physiology
Cell Line
Cells
Chiang John Y L
Cholesterol 7-alpha-Hydroxylase/*genetics
Cultured
Department of Integrative Medical Sciences
DNA Primers
Drug metabolism and disposition: the biological fate of chemicals
Electrophoretic Mobility Shift Assay
Gene Knockdown Techniques
Genetic/*physiology
Han Shuxin
Hepatocytes/*drug effects/enzymology
Humans
Immunoprecipitation
Lithocholic Acid/pharmacology
Messenger/genetics
NEOMED College of Medicine
Polymerase Chain Reaction
Receptors
RNA
Small Interfering
Transcription
Tumor
Two-Hybrid System Techniques
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1099/0022-1317-75-7-1743" target="_blank" rel="noreferrer noopener">http://doi.org/10.1099/0022-1317-75-7-1743</a>
Pages
1743–1747
Volume
75 ( Pt 7)
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Analysis of the thymidine kinase of a herpes simplex virus type 1 isolate that exhibits resistance to (E)-5-(2-bromovinyl)-2'-deoxyuridine.
Publisher
An entity responsible for making the resource available
The Journal of general virology
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-07
Subject
The topic of the resource
Acyclovir/pharmacology; Amino Acid Sequence; Antiviral Agents/metabolism/*pharmacology; Base Sequence; Binding Sites; Bromodeoxyuridine/*analogs & derivatives/metabolism/pharmacology; Deoxyuridine/analogs & derivatives/pharmacology; DNA; Drug Resistance; Herpesvirus 1; Human/drug effects/*enzymology/genetics; Humans; Kinetics; Microbial; Molecular Sequence Data; Phosphonoacetic Acid/pharmacology; Phosphorylation; Point Mutation/genetics; Sequence Analysis; Thymidine Kinase/genetics/metabolism; Thymidine/metabolism; Vidarabine/pharmacology
Creator
An entity primarily responsible for making the resource
Wilber B A; Docherty J J
Description
An account of the resource
The mechanism responsible for the decreased sensitivity of a clinical herpes simplex virus type 1 (HSV-1) isolate, HSV-145, to (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) was examined. Measurements of 50% inhibitory doses of several drugs demonstrated that although HSV-145 was sensitive to phosphonoacetic acid, adenine arabinoside and acyclovir, its sensitivity to BVDU and 5-(2-chloroethyl)-2'-deoxyuridine was significantly less than that normally observed for HSV-1. Analysis of the thymidylate kinase (TMP-K) activity of HSV-145 thymidine kinase (TK) demonstrated a decreased level of TMP-K activity when compared to HSV-1 TK. The TMP-K activity of HSV-145 resembled that observed for HSV-2 and the TK-deficient strain HSV-1 TK-7. When the nucleotide sequence of the HSV-145 TK gene was compared to that of the HSV-1 strains C1(101) and SC16 a single nucleotide substitution (G changed to A at base position 502) was detected which would result in the substitution of threonine at amino acid position 168 for alanine. The substitution is the same as that for the laboratory-derived BVDU-resistant virus HSV-1 SC16B3. Collectively, these studies highlight the importance of amino acid conservation at position 168 of the HSV-1 TK in conferring efficient TMP-K activity and BVDU sensitivity.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1099/0022-1317-75-7-1743" target="_blank" rel="noreferrer noopener">10.1099/0022-1317-75-7-1743</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1994
Acyclovir/pharmacology
Amino Acid Sequence
Antiviral Agents/metabolism/*pharmacology
Base Sequence
Binding Sites
Bromodeoxyuridine/*analogs & derivatives/metabolism/pharmacology
Deoxyuridine/analogs & derivatives/pharmacology
DNA
Docherty J J
Drug Resistance
Herpesvirus 1
Human/drug effects/*enzymology/genetics
Humans
Kinetics
Microbial
Molecular Sequence Data
Phosphonoacetic Acid/pharmacology
Phosphorylation
Point Mutation/genetics
Sequence Analysis
The Journal of general virology
Thymidine Kinase/genetics/metabolism
Thymidine/metabolism
Vidarabine/pharmacology
Wilber B A
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M111553200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M111553200</a>
Pages
11423–11431
Issue
13
Volume
277
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
An N-terminal arginine-rich cluster and a proline-alanine-threonine repeat region determine the cellular localization of the herpes simplex virus type 1 ICP34.5 protein and its ligand, protein phosphatase 1.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-03
Subject
The topic of the resource
*Repetitive Sequences; Alanine/metabolism; Amino Acid; Amino Acid Sequence; Animals; Arginine/metabolism; Base Sequence; Cell Compartmentation; Cercopithecus aethiops; DNA Primers; Fluorescent Antibody Technique; Indirect; Ligands; Molecular Sequence Data; Phosphoprotein Phosphatases/*metabolism; Proline/metabolism; Protein Phosphatase 1; Recombinant Proteins/chemistry/metabolism; Subcellular Fractions/metabolism; Threonine/metabolism; Vero Cells; Viral Proteins/chemistry/*metabolism
Creator
An entity primarily responsible for making the resource
Mao Hanwen; Rosenthal Kenneth S
Description
An account of the resource
The ICP34.5 protein facilitates herpes simplex virus replication by binding and activating protein phosphatase 1 (PP1) by means of a very conserved C-terminal
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M111553200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M111553200</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Repetitive Sequences
2002
Alanine/metabolism
Amino Acid
Amino Acid Sequence
Animals
Arginine/metabolism
Base Sequence
Cell Compartmentation
Cercopithecus aethiops
DNA Primers
Fluorescent Antibody Technique
Indirect
Ligands
Mao Hanwen
Molecular Sequence Data
Phosphoprotein Phosphatases/*metabolism
Proline/metabolism
Protein Phosphatase 1
Recombinant Proteins/chemistry/metabolism
Rosenthal Kenneth S
Subcellular Fractions/metabolism
The Journal of biological chemistry
Threonine/metabolism
Vero Cells
Viral Proteins/chemistry/*metabolism
-
Text
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URL Address
<a href="http://doi.org/10.1074/jbc.M105117200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M105117200</a>
Pages
41690–41699
Issue
45
Volume
276
Dublin Core
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Title
A name given to the resource
Transcriptional regulation of the human sterol 12alpha-hydroxylase gene (CYP8B1): roles of heaptocyte nuclear factor 4alpha in mediating bile acid repression.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-11
Subject
The topic of the resource
*DNA-Binding Proteins; *Transcription; Base Sequence; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Bile Acids and Salts/*pharmacology; Binding Sites; Cholesterol 7-alpha-Hydroxylase/genetics; Cultured; Cytochrome P-450 Enzyme System/*genetics; Cytoplasmic and Nuclear/physiology; Genetic; Genetic/physiology; Hepatocyte Nuclear Factor 4; Humans; Molecular Sequence Data; Phosphoproteins/*physiology; Promoter Regions; Receptors; Repressor Proteins/*pharmacology; Steroid 12-alpha-Hydroxylase; Steroid Hydroxylases/*genetics; Transcription Factors/*physiology; Tumor Cells
Creator
An entity primarily responsible for making the resource
Zhang M; Chiang J Y
Description
An account of the resource
Sterol 12alpha-hydroxylase catalyzes the synthesis of cholic acid and controls the ratio of cholic acid over chenodeoxycholic acid in the bile. Transcription of CYP8B1 is inhibited by bile acids, cholesterol, and insulin. To study the mechanism of CYP8B1 transcription by bile acids, we have cloned and determined 3389 base pairs of the 5'-upstream nucleotide sequences of the human CYP8B1. Deletion analysis of CYP8B1/luciferase reporter activity in HepG2 cells revealed that the sequences from -57 to +300 were important for basal and liver-specific promoter activities. Hepatocyte nuclear factor 4alpha (HNF4alpha) strongly activated human CYP8B1 promoter activities, whereas cholesterol 7alpha-hydroxylase promoter factor (CPF), an NR5A2 family of nuclear receptors, had much less effect. Electrophoretic mobility shift assay identified an overlapping HNF4alpha- and CPF-binding site in the +198/+227 region. The human CYP8B1 promoter activities were strongly repressed by bile acids, and the bile acid response element was localized between +137 and +220. Site-directed mutagenesis of the HNF4alpha-binding site markedly reduced promoter activity and its response to bile acid repression. On the other hand, mutation of the
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An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M105117200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M105117200</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA-Binding Proteins
*Transcription
2001
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Bile Acids and Salts/*pharmacology
Binding Sites
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/genetics
Cultured
Cytochrome P-450 Enzyme System/*genetics
Cytoplasmic and Nuclear/physiology
Department of Integrative Medical Sciences
Genetic
Genetic/physiology
Hepatocyte Nuclear Factor 4
Humans
Molecular Sequence Data
NEOMED College of Medicine
Phosphoproteins/*physiology
Promoter Regions
Receptors
Repressor Proteins/*pharmacology
Steroid 12-alpha-Hydroxylase
Steroid Hydroxylases/*genetics
The Journal of biological chemistry
Transcription Factors/*physiology
Tumor Cells
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.bbrc.2004.02.029" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.bbrc.2004.02.029</a>
Pages
158–164
Issue
1
Volume
316
Dublin Core
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Title
A name given to the resource
Transcriptional regulation of human oxysterol 7alpha-hydroxylase by sterol response element binding protein.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-03
Subject
The topic of the resource
*Transcription Factors; Base Sequence; CCAAT-Enhancer-Binding Proteins/*metabolism; Cytochrome P-450 Enzyme System/*genetics/physiology; Cytochrome P450 Family 7; DNA-Binding Proteins/*metabolism; Enzyme Repression; Genetic; Humans; Molecular Sequence Data; Mutagenesis; Promoter Regions; Response Elements; Sp1 Transcription Factor/antagonists & inhibitors; Steroid Hydroxylases/*genetics/physiology; Sterol Regulatory Element Binding Protein 1; Sterols/metabolism; Transcription
Creator
An entity primarily responsible for making the resource
Norlin Maria; Chiang John Y L
Description
An account of the resource
Oxysterol 7alpha-hydroxylase (CYP7B1) metabolizes oxysterols, potent regulators of lipid homeostasis. Very little is known about transcriptional regulation of human CYP7B1. The present results indicate that sterol response element binding protein (SREBP), a family of oxysterol-responsive transcription factors that stimulates cholesterol synthesis, may be an important regulator of CYP7B1. SREBP suppressed a human CYP7B1 luciferase reporter gene in several cell lines, most markedly in rat hepatoma McA-RH7777 cells. An SREBP-1-responsive region was mapped to a GC-rich sequence in the proximal CYP7B1 promoter, containing binding sites for the basal transcriptional activator Sp1. Mutagenesis of this sequence abolished SREBP-1-mediated suppression. Data indicated that SREBP does not bind this sequence but affects the gene indirectly, probably via interaction with Sp1. Our findings indicate that CYP7B1 transcription is controlled by SREBP and reveal a link between oxysterol-sensitive regulators and oxysterol metabolism. We propose that CYP7B1 is important for regulating cellular sterol content and protects against oxysterol-mediated toxicity.
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<a href="http://doi.org/10.1016/j.bbrc.2004.02.029" target="_blank" rel="noreferrer noopener">10.1016/j.bbrc.2004.02.029</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Transcription Factors
2004
Base Sequence
Biochemical and biophysical research communications
CCAAT-Enhancer-Binding Proteins/*metabolism
Chiang John Y L
Cytochrome P-450 Enzyme System/*genetics/physiology
Cytochrome P450 Family 7
Department of Integrative Medical Sciences
DNA-Binding Proteins/*metabolism
Enzyme Repression
Genetic
Humans
Molecular Sequence Data
Mutagenesis
NEOMED College of Medicine
Norlin Maria
Promoter Regions
Response Elements
Sp1 Transcription Factor/antagonists & inhibitors
Steroid Hydroxylases/*genetics/physiology
Sterol Regulatory Element Binding Protein 1
Sterols/metabolism
Transcription
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0167-4781(93)90274-h" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0167-4781(93)90274-h</a>
Pages
95–100
Issue
1
Volume
1172
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Differential expression of the mRNAs for the soluble and membrane-bound forms of rabbit cytochrome b5.
Publisher
An entity responsible for making the resource available
Biochimica et biophysica acta
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-02
Subject
The topic of the resource
Agar Gel; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cell Membrane/metabolism; Cytochromes b5/*genetics; Cytosol/metabolism; DNA/genetics/isolation & purification; Electrophoresis; Exons; Leukocytes/metabolism; Liver/*metabolism; Messenger/genetics/isolation & purification/*metabolism; Molecular Sequence Data; Oligodeoxyribonucleotides; Polymerase Chain Reaction/methods; Rabbits; Reticulocytes/metabolism; RNA; Southern
Creator
An entity primarily responsible for making the resource
Giordano S J; Steggles A W
Description
An account of the resource
Total RNA was extracted from a variety of rabbit tissues and reverse transcribed for use in the polymerase chain reaction technique. Using primers designed to amplify the membrane-bound liver cytochrome b5 cDNA, products of two sizes were observed. Both hybridized strongly to a radiolabelled liver cytochrome b5 probe. Sequencing confirmed that the two types of cDNA product encoded the membrane-bound and the soluble forms of b5. Messenger RNA corresponding to the soluble cytochrome was detected in the lung, gallbladder and the adrenal gland, as well as in reticulocytes and bone marrow. This was an unexpected finding since the protein has been isolated only from erythrocytes. In contrast, membrane-bound cytochrome b5 mRNA was detected in all tissues tested, suggesting that the corresponding protein is ubiquitous in tissue distribution.
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<a href="http://doi.org/10.1016/0167-4781(93)90274-h" target="_blank" rel="noreferrer noopener">10.1016/0167-4781(93)90274-h</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
Agar Gel
Amino Acid Sequence
Animals
Base Sequence
Biochimica et biophysica acta
Blotting
Cell Membrane/metabolism
Cytochromes b5/*genetics
Cytosol/metabolism
DNA/genetics/isolation & purification
Electrophoresis
Exons
Giordano S J
Leukocytes/metabolism
Liver/*metabolism
Messenger/genetics/isolation & purification/*metabolism
Molecular Sequence Data
Oligodeoxyribonucleotides
Polymerase Chain Reaction/methods
Rabbits
Reticulocytes/metabolism
RNA
Southern
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0167-4781(92)90535-8" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0167-4781(92)90535-8</a>
Pages
227–228
Issue
2
Volume
1130
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Nucleotide sequence of a region duplicated in Escherichia coli toc mutants.
Publisher
An entity responsible for making the resource available
Biochimica et biophysica acta
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
1992-03
Subject
The topic of the resource
*Genes; *Multigene Family; Bacterial; Base Sequence; DNA Topoisomerases; Escherichia coli/*genetics; Molecular Sequence Data; Type I/genetics
Creator
An entity primarily responsible for making the resource
Yang T P; Depew R E
Description
An account of the resource
We have sequenced about 5 kb of the Escherichia coli chromosome downstream from the tolC gene, looking for a topoisomerase gene. This region does not contain a topoisomerase gene.
Identifier
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<a href="http://doi.org/10.1016/0167-4781(92)90535-8" target="_blank" rel="noreferrer noopener">10.1016/0167-4781(92)90535-8</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Genes
*Multigene Family
1992
Bacterial
Base Sequence
Biochimica et biophysica acta
Depew R E
DNA Topoisomerases
Escherichia coli/*genetics
Molecular Sequence Data
Type I/genetics
Yang T P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0006-291x(91)91776-9" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0006-291x(91)91776-9</a>
Pages
38–44
Issue
1
Volume
178
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The human liver and reticulocyte cytochrome b5 mRNAs are products from a single gene.
Publisher
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Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1991
1991-07
Subject
The topic of the resource
*Genes; Amino Acid Sequence; Base Sequence; Cytochromes b5/blood/*genetics; Gene Library; Genetic; Humans; Liver/*metabolism; Molecular Sequence Data; Polymerase Chain Reaction; Polymorphism; Reticulocytes/*metabolism; RNA/blood/genetics/isolation & purification
Creator
An entity primarily responsible for making the resource
Giordano S J; Steggles A W
Description
An account of the resource
Using a combination of standard cDNA library screening techniques and the Polymerase Chain Reaction (PCR) we have isolated and sequenced DNA fragments corresponding to the human reticulocyte cytochrome b5 mRNA. The reticulocyte specific sequence codes for amino acids 97 and 98 only, with a TAA stop codon. The reticulocyte specific 3'non-translated sequence has 15 new nucleotides then utilizes the liver mRNA sequence from amino acid 97 onward. This indicates that the reticulocyte specific exon has 24 base pairs (bp). In addition, we have isolated sequences that are derived from a transcribed cytochrome b5 pseudogene. This transcript contains multiple mutations which prevent the synthesis of any functional protein.
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An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0006-291x(91)91776-9" target="_blank" rel="noreferrer noopener">10.1016/0006-291x(91)91776-9</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Genes
1991
Amino Acid Sequence
Base Sequence
Biochemical and biophysical research communications
Cytochromes b5/blood/*genetics
Gene Library
Genetic
Giordano S J
Humans
Liver/*metabolism
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism
Reticulocytes/*metabolism
RNA/blood/genetics/isolation & purification
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s00360-016-1029-6" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s00360-016-1029-6</a>
Pages
235–252
Issue
1
Volume
187
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Beyond thermoregulation: metabolic function of cetacean blubber in migrating bowhead and beluga whales.
Publisher
An entity responsible for making the resource available
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-01
Subject
The topic of the resource
*Lipid Metabolism; Adipose Tissue/*metabolism; Aging/metabolism; Amino Acid Sequence; Animals; Base Sequence; Beluga Whale/*physiology; Blubber; Body Temperature Regulation; Bowhead whale; Bowhead Whale/*physiology; Development; Female; Humans; Inbred C57BL; Leptin; Leptin/genetics; Leptin/genetics/metabolism; Lipase/genetics; Long-Evans; Male; Metabolic activity; Mice; Rats; Receptors; Seasons
Creator
An entity primarily responsible for making the resource
Ball H C; Londraville R L; Prokop J W; George John C; Suydam R S; Vinyard C; Thewissen J G M; Duff R J
Description
An account of the resource
The processes of lipid deposition and utilization, via the gene leptin (Lep), are poorly understood in taxa with varying degrees of adipose storage. This study examines how these systems may have adapted in marine aquatic environments inhabited by cetaceans. Bowhead (Balaena mysticetus) and beluga whales (Delphinapterus leucas) are ideal study animals-they possess large subcutaneous adipose stores (blubber) and undergo bi-annual migrations concurrent with variations in food availability. To answer long-standing questions regarding how (or if) energy and lipid utilization adapted to aquatic stressors, we quantified variations in gene transcripts critical to lipid metabolism related to season, age, and blubber depth. We predicted leptin tertiary structure conservation and assessed inter-specific variations in Lep transcript numbers between bowheads and other mammals. Our study is the first to identify seasonal and age-related variations in Lep and lipolysis in these cetaceans. While Lep transcripts and protein oscillate with season in adult bowheads reminiscent of hibernating mammals, transcript levels reach up to 10 times higher in bowheads than any other mammal. Data from immature bowheads are consistent with the hypothesis that short baleen inhibits efficient feeding. Lipolysis transcripts also indicate young Fall bowheads and those sampled during Spring months limit energy utilization. These novel data from rarely examined species expand the existing knowledge and offer unique insight into how the regulation of Lep and lipolysis has adapted to permit seasonal deposition and maintain vital blubber stores.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s00360-016-1029-6" target="_blank" rel="noreferrer noopener">10.1007/s00360-016-1029-6</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Lipid Metabolism
2017
Adipose Tissue/*metabolism
Aging/metabolism
Amino Acid Sequence
Animals
Ball H C
Base Sequence
Beluga Whale/*physiology
Blubber
Body Temperature Regulation
bowhead whale
Bowhead Whale/*physiology
Department of Anatomy & Neurobiology
Department of Family & Community Medicine
development
Duff R J
Female
George John C
Humans
Inbred C57BL
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
leptin
Leptin/genetics
Leptin/genetics/metabolism
Lipase/genetics
Londraville R L
Long-Evans
Male
Metabolic activity
Mice
NEOMED College of Medicine
Prokop J W
Rats
Receptors
Seasons
Suydam R S
Thewissen J G M
Vinyard C
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/bf00944199" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf00944199</a>
Pages
21–26
Issue
1
Volume
139
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Induction of ethanol dependence increases signal peptidase mRNA levels in rat brain.
Publisher
An entity responsible for making the resource available
Molecular and cellular biochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-10
Subject
The topic of the resource
*Membrane Proteins; *Serine Endopeptidases; Alcoholism/*enzymology; Amino Acid; Amino Acid Sequence; Animals; Base Sequence; Blotting; Brain/*enzymology; Complementary; DNA; Endopeptidases/*biosynthesis/chemistry/genetics; Ethanol/*pharmacology; Male; Messenger/genetics/metabolism; Molecular Sequence Data; Northern; Nucleic Acid; Rats; RNA; Sequence Homology; Sprague-Dawley; Substance Withdrawal Syndrome/enzymology
Creator
An entity primarily responsible for making the resource
Signs S A; Jacquet R
Description
An account of the resource
Differential Northern blot hybridization was used as a screening tool to identify mRNAs that respond quantitatively to the induction of ethanol dependence. Adult male rats were treated with repeated, high doses of ethanol for 4 consecutive days. This regimen resulted in the development of tolerance and dependence upon ethanol. RNA isolated from the ethanol-dependent rat brains was used to construct a cDNA library. One cDNA was identified that hybridized to a mRNA which increased in rat brain during the ethanol treatment. Sequence analysis of the cDNA indicated that it recognized a mRNA in rat brain which was very similar to that which encodes the 18 kDa subunit of canine signal peptidase. The rat signal peptidase mRNA was observed to increase in brain nearly 2-fold within 48 h after the initiation of ethanol treatment. Ethanol did not significantly alter beta-actin mRNA levels during the treatment period. These results support the existence of an ethanol-responsive signal peptidase mRNA in rat brain.
Identifier
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<a href="http://doi.org/10.1007/bf00944199" target="_blank" rel="noreferrer noopener">10.1007/bf00944199</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Membrane Proteins
*Serine Endopeptidases
1994
Alcoholism/*enzymology
Amino Acid
Amino Acid Sequence
Animals
Base Sequence
Blotting
Brain/*enzymology
Complementary
DNA
Endopeptidases/*biosynthesis/chemistry/genetics
Ethanol/*pharmacology
Jacquet R
Male
Messenger/genetics/metabolism
Molecular and cellular biochemistry
Molecular Sequence Data
Northern
Nucleic Acid
Rats
RNA
Sequence Homology
Signs S A
Sprague-Dawley
Substance Withdrawal Syndrome/enzymology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/bf00202825" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf00202825</a>
Pages
568–570
Issue
5
Volume
93
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A splicing mutation in the cytochrome b5 gene from a patient with congenital methemoglobinemia and pseudohermaphrodism.
Publisher
An entity responsible for making the resource available
Human genetics
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-05
Subject
The topic of the resource
*DNA; *Mutation; Base Sequence; Cytochromes b5/*genetics; Disorders of Sex Development/*enzymology/genetics; DNA Primers/chemistry; Gene Deletion; Humans; Leukocytes/metabolism; Male; Messenger/metabolism; Methemoglobinemia/*congenital/*enzymology; Molecular Sequence Data; Polymerase Chain Reaction; Recombinant; Reticulocytes/metabolism; RNA
Creator
An entity primarily responsible for making the resource
Giordano S J; Kaftory A; Steggles A W
Description
An account of the resource
We have analyzed reticulocyte and leukocyte mRNAs isolated from a patient with congenital methemoglobinemia and pseudohermaphrodism. The cytochrome b5 cDNA sequences were amplified using specific oligonucleotide primers and the polymerase chain reaction (PCR). DNA sequencing indicated that there was a 16-bp deletion in the cDNA leading to a new, in-frame stop signal and resulting in a truncated protein of 45 amino acids. Genomic DNA was analyzed, and the molecular lesion was shown to be an AG–\textgreaterGG alteration in the 3' splicing junction of intron 1. The splice site alteration leads to the usage of the nearest AG as an alternative splice site, resulting in a 16-bp deletion in the mRNA. All of the studies on reticulocyte mRNA and genomic DNA indicated that the patient was homozygous for the lesion.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/bf00202825" target="_blank" rel="noreferrer noopener">10.1007/bf00202825</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA
*Mutation
1994
Base Sequence
Cytochromes b5/*genetics
Disorders of Sex Development/*enzymology/genetics
DNA Primers/chemistry
Gene Deletion
Giordano S J
Human genetics
Humans
Kaftory A
Leukocytes/metabolism
Male
Messenger/metabolism
Methemoglobinemia/*congenital/*enzymology
Molecular Sequence Data
Polymerase Chain Reaction
Recombinant
Reticulocytes/metabolism
RNA
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/geno.1994.1177" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/geno.1994.1177</a>
Pages
320–323
Issue
2
Volume
20
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Structure and nucleotide sequences of the human cholesterol 7 alpha-hydroxylase gene (CYP7).
Publisher
An entity responsible for making the resource available
Genomics
Date
A point or period of time associated with an event in the lifecycle of the resource
1994
1994-03
Subject
The topic of the resource
Amino Acid Sequence; Base Sequence; Cholesterol 7-alpha-Hydroxylase/chemistry/*genetics; DNA; Humans; Molecular Sequence Data; Restriction Mapping
Creator
An entity primarily responsible for making the resource
Wang D P; Chiang J Y
Description
An account of the resource
The human cholesterol 7 alpha-hydroxylase gene (CYP7) spans about 11 kb of the genome and contains six exons and five introns. Nucleotide sequences of a 5'-upstream region to exon III (5535 bp), a 5'-upstream EcoRI fragment (2575 bp), and an EcoRI fragment covering intron V to exon VI (2319 bp) have been determined. A comparison of our sequences with those reported previously unveiled numerous sequencing discrepancies that are apparently due to sequencing errors. There are only one confirmed and one possible genetic polymorphism in the promoter of this highly conserved human gene. The proximal promoter contained many consensus recognition sequences for liver-enriched transcription factors and steroid hormone receptors that may play important roles in regulation of the CYP7 gene transcription by bile acids, cholesterol, and hormones.
Identifier
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<a href="http://doi.org/10.1006/geno.1994.1177" target="_blank" rel="noreferrer noopener">10.1006/geno.1994.1177</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1994
Amino Acid Sequence
Base Sequence
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/chemistry/*genetics
Department of Integrative Medical Sciences
DNA
Genomics
Humans
Molecular Sequence Data
NEOMED College of Medicine
Restriction Mapping
Wang D P
-
Text
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URL Address
<a href="http://doi.org/10.1006/geno.1993.1331" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/geno.1993.1331</a>
Pages
348–354
Issue
2
Volume
17
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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The isolation and characterization of the bovine cytochrome b5 gene, and a transcribed pseudogene.
Publisher
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Genomics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-08
Subject
The topic of the resource
*Pseudogenes; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cattle/*genetics; Cytochromes b5/*genetics; DNA/genetics/isolation & purification; Exons; Gene Expression; Genetic; Genomic Library; Humans; Introns; Messenger/biosynthesis/metabolism; Molecular Sequence Data; Nucleic Acid; Nucleic Acid Conformation; Oligodeoxyribonucleotides; Rabbits; Restriction Mapping; Reticulocytes/metabolism; RNA; RNA Precursors/chemistry/metabolism; Sequence Homology; Southern; Transcription
Creator
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Cristiano R J; Giordano S J; Steggles A W
Description
An account of the resource
This is the first isolation and characterization of a cytochrome b5(b5) gene. The bovine b5 gene is quite large, spanning about 28 kb and contains six exons. One of these exons appears to code for a reticulocyte-specific sequence similar to that described for human and rabbit b5. All of the splicing junctions conform to the GT-AG consensus rule. The 5' flanking sequence has no obvious TATA box, two CAAT boxes, and contains several G:C-rich SpI motifs indicative of a house-keeping gene. In reticulocyte mRNA we found evidence for a transcribed b5 pseudogene, but could not detect sequences coding for the soluble form of b5. We conclude that the soluble form of b5 is derived from the membrane-bound b5 by a post-translational mechanism.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/geno.1993.1331" target="_blank" rel="noreferrer noopener">10.1006/geno.1993.1331</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Pseudogenes
1993
Amino Acid Sequence
Animals
Base Sequence
Blotting
Cattle/*genetics
Cristiano R J
Cytochromes b5/*genetics
DNA/genetics/isolation & purification
Exons
Gene Expression
Genetic
Genomic Library
Genomics
Giordano S J
Humans
Introns
Messenger/biosynthesis/metabolism
Molecular Sequence Data
Nucleic Acid
Nucleic Acid Conformation
Oligodeoxyribonucleotides
Rabbits
Restriction Mapping
Reticulocytes/metabolism
RNA
RNA Precursors/chemistry/metabolism
Sequence Homology
Southern
Steggles A W
Transcription
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/bbrc.1997.7599" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1997.7599</a>
Pages
80–83
Issue
1
Volume
240
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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The isolation and characterization of the soluble and membrane-bound porcine cytochrome b5 cDNAs.
Publisher
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Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1997-11
Subject
The topic of the resource
Amino Acid Sequence; Animals; Base Sequence; Blotting; Complementary/blood/*chemistry/*isolation & purification; Cytochromes b5/blood/*chemistry/genetics/*isolation & purification; DNA; Male; Membrane Proteins/*chemistry/genetics/*isolation & purification; Molecular Sequence Data; Solubility; Southern; Swine; Testis/enzymology
Creator
An entity primarily responsible for making the resource
VanDerMark P K; Steggles A W
Description
An account of the resource
In some male pigs, there is an increased production of the testicular 16 androstene steroids which end up being concentrated in fatty tissue. When the meat is cooked, a disagreeable odor/flavor is produced, a phenomenon known as "boar taint." All boars selected for food production are castrated even though only ca 10% of boars may be "tainted." This has a high economic cost because castrated pigs convert food into meat less efficiently, the meat is fatter, and there is an increased mortality due to the castration procedure. Recent data has implicated an increased level of cytochrome b5 in the testes with the increased synthesis of the 16-androstene steroids. As an initial step in analyzing this process, we used 5' and 3' RACE PCR procedures to isolate, clone and sequence the cDNAs for the membrane-bound and soluble forms of porcine cytochrome b5.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/bbrc.1997.7599" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1997.7599</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1997
Amino Acid Sequence
Animals
Base Sequence
Biochemical and biophysical research communications
Blotting
Complementary/blood/*chemistry/*isolation & purification
Cytochromes b5/blood/*chemistry/genetics/*isolation & purification
DNA
Male
Membrane Proteins/*chemistry/genetics/*isolation & purification
Molecular Sequence Data
Solubility
Southern
Steggles A W
Swine
Testis/enzymology
VanDerMark P K