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Text
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URL Address
<a href="http://doi.org/10.1006/bbrc.1998.9759" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1998.9759</a>
Pages
109–113
Issue
1
Volume
253
Dublin Core
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Title
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Basic transcription element binding protein (BTEB) transactivates the cholesterol 7 alpha-hydroxylase gene (CYP7A).
Publisher
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Biochemical and biophysical research communications
Date
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1998
1998-12
Subject
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*Gene Expression Regulation; Animals; Bile Acids and Salts/genetics; Binding Sites/genetics; Carcinoma; Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism; Complementary/analysis; Cultured; DNA; DNA-Binding Proteins/metabolism/*physiology; Electrophoresis; Enzyme Activation/genetics; Genetic; Hepatocellular; Humans; Kruppel-Like Transcription Factors; Liver/enzymology; Podophyllin/analogs & derivatives/metabolism; Podophyllotoxin/analogs & derivatives; Polyacrylamide Gel; Promoter Regions; Protein Binding/genetics; Rats; Transcription Factors/metabolism/*physiology; Tumor Cells
Creator
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Foti D; Stroup D; Chiang J Y
Description
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Cholesterol 7 alpha-hydroxylase catalyzes the first and rate-limiting step in the conversion of cholesterol to bile acids in the liver. Previously, we have identified two bile acid response elements located in nt -74 to -54 (BARE-I) and -148 to -118 (BARE-II) regions. The nucleotide sequences in these BAREs are highly conserved and shared a novel sequence, AGTTCAAG. To identify and isolate nuclear protein factors that bind to these BAREs, we have screened a human liver cDNA expression library with oligonucleotide probes containing the sequence from nt -149 to -127. Twenty positive clones were selected and purified. Partial nucleotide sequences of these clones were determined. Nucleotide homology search of DNA databases of the sequences of these clones revealed that sequence of one clone, G13, is identical to basic transcription element binding protein (BTEB), a GC box-binding protein of Sp1 family transcription factors known to regulate many cytochrome P450 genes. Electrophoretic mobility shift assays have identified a basic transcription element (BTE) in BARE-II and a Sp1 binding site located in the nt -100/-82 region of the CYP7A promoter. Transient transfection assays have confirmed that BTEB was able to transactivate the CYP7A promoter/luciferase chimergic gene.
Identifier
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<a href="http://doi.org/10.1006/bbrc.1998.9759" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1998.9759</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
1998
Animals
Bile Acids and Salts/genetics
Binding Sites/genetics
Biochemical and biophysical research communications
Carcinoma
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics/metabolism
Complementary/analysis
Cultured
Department of Integrative Medical Sciences
DNA
DNA-Binding Proteins/metabolism/*physiology
Electrophoresis
Enzyme Activation/genetics
Foti D
Genetic
Hepatocellular
Humans
Kruppel-Like Transcription Factors
Liver/enzymology
NEOMED College of Medicine
Podophyllin/analogs & derivatives/metabolism
Podophyllotoxin/analogs & derivatives
Polyacrylamide Gel
Promoter Regions
Protein Binding/genetics
Rats
Stroup D
Transcription Factors/metabolism/*physiology
Tumor Cells