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Text
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<a href="http://doi.org/10.1016/j.ijporl.2013.11.028" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.ijporl.2013.11.028</a>
Pages
416–422
Issue
3
Volume
78
Dublin Core
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Title
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Effects of FGF-2 and OP-1 in vitro on donor source cartilage for auricular reconstruction tissue engineering.
Publisher
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International journal of pediatric otorhinolaryngology
Date
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2014
2014-03
Subject
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Bone Morphogenetic Protein 7/*pharmacology; Cell Differentiation/drug effects; Cells; Child; Chondrocytes; Chondrocytes/*drug effects/physiology; Congenital Abnormalities/diagnosis/*surgery; Congenital Microtia; Cultured; Ear Cartilage/surgery; Ear/*abnormalities/surgery; FGF-2; Fibroblast Growth Factor 2/*pharmacology; Humans; Male; Microtia; OP-1; Reconstructive Surgical Procedures/*methods; Reference Values; Tissue Donors; Tissue engineering; Tissue Engineering/*methods
Creator
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Shasti Mark; Jacquet Robin; McClellan Phillip; Yang Julianne; Matsushima Seika; Isogai Noritaka; Murthy Ananth; Landis William J
Description
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OBJECTIVE: Microtia is a congenital partial or total loss of the external ear with current treatment approaches involving autologous construction from costal cartilage. Alternatively, tissue engineering provides possible use of normal or microtia auricular chondrocytes harvested from patients. This study investigated effects in vitro of basic fibroblast growth factor (FGF-2) and osteogenic protein 1 (OP-1) on human pediatric normal and microtia auricular chondrocytes and their potential proliferation and differentiation for cellular expansion. A working hypothesis was that FGF-2 promotes proliferation and OP-1 maintains an auricular phenotype of these cells. METHODS: Two patients, one undergoing otoplasty and one an ear construction, yielded normal and microtia auricular chondrocytes, respectively. The two donor sets of isolated chondrocytes were equally divided into four experimental cell groups. These were controls without added growth factors and cells supplemented with FGF-2, OP-1 or FGF-2/OP-1 combined. Cells were cultured 3, 5, 7, and 10 days (3 replicates/time point), counted and assayed by RT-qPCR to determine elastin and types II and III collagen gene expression. RESULTS: Compared to control counterparts, normal and microtia chondrocytes with
Identifier
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<a href="http://doi.org/10.1016/j.ijporl.2013.11.028" target="_blank" rel="noreferrer noopener">10.1016/j.ijporl.2013.11.028</a>
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2014
Bone Morphogenetic Protein 7/*pharmacology
Cell Differentiation/drug effects
Cells
Child
Chondrocytes
Chondrocytes/*drug effects/physiology
Congenital Abnormalities/diagnosis/*surgery
Congenital Microtia
Cultured
Ear Cartilage/surgery
Ear/*abnormalities/surgery
FGF-2
Fibroblast Growth Factor 2/*pharmacology
Humans
International journal of pediatric otorhinolaryngology
Isogai Noritaka
Jacquet Robin
Landis William J
Male
Matsushima Seika
McClellan Phillip
Microtia
Murthy Ananth
OP-1
Reconstructive Surgical Procedures/*methods
Reference Values
Shasti Mark
Tissue Donors
Tissue Engineering
Tissue Engineering/*methods
Yang Julianne