Activation Of The Interferon-inducing And Anti-viral Activities Of Polyribonucleotides By Intercalating Drugs
Biophysics
Jamison J M; Bonilla P J; Koo P H; Tsai C C
Biophysical Journal
1985
1985
Journal Article or Conference Abstract Publication
n/a
Effects Of Intercalating Drugs On The Antiviral And Interferon-inducing Activities Of Polyribonucleotides
Biophysics
Jamison J M; Bonilla P J; Tsai C C
Biophysical Journal
1986
1986-02
Journal Article or Conference Abstract Publication
n/a
Enhancement Of The Antiviral Activity Of Poly (a-u) By Adriamycin And Daunomycin
Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Virology
The role of adriamycin (ADR) and daunomycin (DMN) in modulating the antiviral activity of poly (adeylate-uridylate) (poly (A-U)) was examined using a human foreskin fibroblast - vesicular stomatitis virus (HSF-VSV) bioassay in which the concentration of poly (A-U) was fixed at 0.05 mm or 0.2 mm while the ADR or DMN concentration was varied to produce ADR (or DMN)/ribonucleotide ratios ranging from 1:16 to 2:1. Poly (A-U), ADR and DMN were not efficacious antiviral agents when tested individually at the concentrations employed in the ADR (or DMN)/poly (A-U) combinations. When the ADR or DMN was combined with the poly (A-U) to produce ADR (or DMN)/poly (A-U) ratios of 1/6, the 50% effective doses (ED50) of the poly (A-U), ADR and DMN decreased 18, 104, and 185-fold, respectively. However, when ADR or DMN was combined with polyriboinosinic-polyribocytidylic acid [poly (I) . poly (C)], the ED50 of the ADR, DMN and the poly (I) . poly (C) were not affected. Interferon neutralization studies indicated that ADR, DMN, poly (A-U) and the ADR (or DMN)/poly (A-U) combination induced the production of interferon-beta (IFN-beta). The amount of IFN produced by the ADR (or DMN)/poly (A-U) combinations was equal to the sum of the IFN produced by their constituents. These results indicate that the ADR and DMN potentiate the antiviral activity of the poly (A-U) without affecting the amount of IFN induced. The direct viral inactivation study demonstrated that ADR, DMN, poly (A-U) and the ADR (or DMN)/poly (A-U) combinations do not inactivate the VSV at concentrations near the ED50.
Jamison J M; Bonilla P J; Tsai C C
Antiviral Chemistry & Chemotherapy
1990
1990-10
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1177/095632029000100502" target="_blank" rel="noreferrer noopener">10.1177/095632029000100502</a>
Invitro Antiviral Activity Induced By Poly-r(a-u) And Intercalating Dyes
Biophysics
Jamison J M; Bonilla P J; Flowers D G; Kitareewan S; Tsai C C
Biophysical Journal
1987
1987-02
Journal Article or Conference Abstract Publication
n/a
Modulation Of The Antiviral Activity Of Poly (a-u) By Ethidium Bromide And Propidium Iodide
Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Virology
The role of ethidium bromide (EB) and propidium iodide (PI) in modulating the antiviral and interferon-inducing activities of poly(adenylate-uridylate) (poly (A-U )) was examined using the human foreskin fibro-blast-vesicular stomatitis virus (HSF-VSV) bioassay system in which the concentration of poly (A-U) was fixed at 0.05 mM or 0.2 mM while the EB or PI concentration was varied to produce variable EB (or PI)/ribonucleotide ratios ranging from 1: 16 to 2:1. EB, PI and poly (A-U) tested individually were not efficacious antiviral agents. When poly (A-U) was combined with the ethidium bromide or propidium iodide the antiviral activity was potentiated 15- to 22-fold at EB (or Pl)/ribonucleotide ratios in the region of 1/4. The interferon-inducing activity of the EB (or Pl)/poly (A-U) combinations were equal to the sum of the interferon-inducing activity of the poly (A-U) and the EB or (PI). These results indicate that the EB and PI potentiate the antiviral activity of the poly (A-U) without superinduction of interferon. The direct viral inactivation study demonstrated that EB, PI, poly (A-U) and the EB (or Pl)/poly (A-U) combinations did not inactivate the VSV at concentrations near the 50% viral inhibitory dose.
Jamison J M; Bonilla P J; Tsai C C
Antiviral Chemistry & Chemotherapy
1990
1990-02
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1177/095632029000100109" target="_blank" rel="noreferrer noopener">10.1177/095632029000100109</a>