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<a href="http://doi.org/10.1007/978-1-0716-1119-7_15" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/978-1-0716-1119-7_15</a>
Pages
215-224
Volume
2245
ISSN
1940-6029 1064-3745
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Update Year & Number
January 2021 List
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Dublin Core
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Title
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Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.
Publisher
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Methods in Molecular Biology
Date
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2021
1905-07
Subject
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Inflammation; Mitochondria; Oxidative stress; Osteoarthritis; Chondrocytes; Inflammation; Osteoarthritis; Autophagy; CCCP; Confocal microscopy; IL-1β; Immunofluorescence; Mitochondrial dysfunction; Mitophagy; MitoTracker Deep Red; Parkin; PINK1; Autophagy; CCCP; Chondrocytes; Confocal microscopy; IL-1β; Immunofluorescence; Mitochondria; Mitochondrial dysfunction; Mitophagy; MitoTracker Deep Red; Oxidative stress; Parkin; PINK1
Creator
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Ansari MY; Haqqi TM
Description
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Immunofluorescence staining is a widely used and powerful tool for the visualization and colocalization of two or more proteins and/or cellular organelles. For colocalization studies in fixed cells, one target protein/organelle is immunostained and visualized by one fluorophore and the other target protein/organelle is immunostained and visualized by a different fluorophore whose excitation emission spectra does not overlap with the first fluorophore. Parkin (PARK2) is an E3 ubiquitin ligase which performs ubiquitination of surface proteins of dysfunctional mitochondria to mark them for autolysosomal degradation. Here we describe the immunofluorescence staining of parkin protein and immunofluorescence or dye-based methods to visualize mitochondria and study the colocalization of parkin and mitochondria in primary human or mouse chondrocytes or cell lines.
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<a href="http://doi.org/10.1007/978-1-0716-1119-7_15" target="_blank" rel="noreferrer noopener">10.1007/978-1-0716-1119-7_15</a>
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journalArticle
2021
Ansari MY
Autophagy
CCCP
Chondrocytes
Confocal microscopy
Department of Anatomy & Neurobiology
Haqqi TM
IL-1β
immunofluorescence
Inflammation
January 2021 List
journalArticle
Methods in Molecular Biology
Mitochondria
Mitochondrial dysfunction
mitophagy
MitoTracker Deep Red
NEOMED College of Medicine
Osteoarthritis
Oxidative Stress
Parkin
PINK1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-1119-7_15"><span>https://doi.org/10.1007/978-1-0716-1119-7_15</span></a>
ISSN
978-1-0716-1119-7
NEOMED College
NEOMED College of Medicine
NEOMED Department
Department of Anatomy & Neurobiology
Update Year & Number
Jan to Aug list 2021
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Assessing chondrocyte status by immunofluorescence-mediated localization of parkin relative to mitochondria.
Creator
An entity primarily responsible for making the resource
Ansari MY; Haqqi TM
Publisher
An entity responsible for making the resource available
Methods in Molecular Biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2020
2020-12-15
Subject
The topic of the resource
Immunofluorescence staining is a widely used and powerful tool for the visualization and colocalization of two or more proteins and/or cellular organelles. For colocalization studies in fixed cells, one target protein/organelle is immunostained and visualized by one fluorophore and the other target protein/organelle is immunostained and visualized by a different fluorophore whose excitation emission spectra does not overlap with the first fluorophore. Parkin (PARK2) is an E3 ubiquitin ligase which performs ubiquitination of surface proteins of dysfunctional mitochondria to mark them for autolysosomal degradation. Here we describe the immunofluorescence staining of parkin protein and immunofluorescence or dye-based methods to visualize mitochondria and study the colocalization of parkin and mitochondria in primary human or mouse chondrocytes or cell lines.
Identifier
An unambiguous reference to the resource within a given context
<a href="https://link.springer.com/protocol/10.1007/978-1-0716-1119-7_15"><span>https://doi.org/10.1007/978-1-0716-1119-7_15</span></a>
Rights
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© Springer Science+Business Media, LLC, part of Springer Nature 2021
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2020
Autophagy
CCCP
Chondrocytes
Confocal microscopy
IL-1β
immunofluorescence
Inflammation
Mitochondria
Mitochondrial dysfunction
mitophagy
MitoTracker Deep Red
Osteoarthritis
Oxidative Stress
Parkin
PINK1