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URL Address
<a href="http://doi.org/10.1006/geno.1993.1331" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/geno.1993.1331</a>
Pages
348–354
Issue
2
Volume
17
Dublin Core
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Title
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The isolation and characterization of the bovine cytochrome b5 gene, and a transcribed pseudogene.
Publisher
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Genomics
Date
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1993
1993-08
Subject
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*Pseudogenes; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cattle/*genetics; Cytochromes b5/*genetics; DNA/genetics/isolation & purification; Exons; Gene Expression; Genetic; Genomic Library; Humans; Introns; Messenger/biosynthesis/metabolism; Molecular Sequence Data; Nucleic Acid; Nucleic Acid Conformation; Oligodeoxyribonucleotides; Rabbits; Restriction Mapping; Reticulocytes/metabolism; RNA; RNA Precursors/chemistry/metabolism; Sequence Homology; Southern; Transcription
Creator
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Cristiano R J; Giordano S J; Steggles A W
Description
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This is the first isolation and characterization of a cytochrome b5(b5) gene. The bovine b5 gene is quite large, spanning about 28 kb and contains six exons. One of these exons appears to code for a reticulocyte-specific sequence similar to that described for human and rabbit b5. All of the splicing junctions conform to the GT-AG consensus rule. The 5' flanking sequence has no obvious TATA box, two CAAT boxes, and contains several G:C-rich SpI motifs indicative of a house-keeping gene. In reticulocyte mRNA we found evidence for a transcribed b5 pseudogene, but could not detect sequences coding for the soluble form of b5. We conclude that the soluble form of b5 is derived from the membrane-bound b5 by a post-translational mechanism.
Identifier
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<a href="http://doi.org/10.1006/geno.1993.1331" target="_blank" rel="noreferrer noopener">10.1006/geno.1993.1331</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Pseudogenes
1993
Amino Acid Sequence
Animals
Base Sequence
Blotting
Cattle/*genetics
Cristiano R J
Cytochromes b5/*genetics
DNA/genetics/isolation & purification
Exons
Gene Expression
Genetic
Genomic Library
Genomics
Giordano S J
Humans
Introns
Messenger/biosynthesis/metabolism
Molecular Sequence Data
Nucleic Acid
Nucleic Acid Conformation
Oligodeoxyribonucleotides
Rabbits
Restriction Mapping
Reticulocytes/metabolism
RNA
RNA Precursors/chemistry/metabolism
Sequence Homology
Southern
Steggles A W
Transcription