1
40
9
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s10461-006-9069-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s10461-006-9069-7</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
253-261
Issue
3
Volume
10
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The differential impact of PTSD and depression on HIV disease markers and adherence to HAART in people living with HIV
Publisher
An entity responsible for making the resource available
Aids and Behavior
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-05
Subject
The topic of the resource
depression; social support; HIV; adherence; PTSD; Environmental & Occupational Health; Public; Biomedical Social Sciences; posttraumatic-stress-disorder; primary care; medication; drug-resistance; protease inhibitors; active antiretroviral therapy; CD4; cell count; outpatient clinics; viral load
Creator
An entity primarily responsible for making the resource
Boarts J M; Sledjeski E M; Bogart L M; Delahanty D L
Description
An account of the resource
Despite high rates of comorbidity, research has typically focused on the independent impact of posttraumatic stress disorder (PTSD) and depression symptoms in people living with HIV (PLWH). The present study examined the independent and comorbid influence of PTSD and depression symptoms on medication adherence, CD4 cell counts, and viral load, over the course of 3 months in 57 PLWH (82% men, 54% Caucasian, 44% African American) recruited from a clinic or social service agency. Both PTSD and depressive symptoms predicted lower subsequent adherence. However, only depressive symptoms predicted lower CD4 counts and presence of a detectable viral load. Participants reporting symptoms consistent with diagnostic levels of comorbid PTSD and depression were less likely to adhere to HAART and were more likely to have a detectable viral load. These results highlight the influences of PTSD and depression on adherence and HIV disease markers, and underscore the importance of examining comorbid symptomatology in PLWH.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s10461-006-9069-7" target="_blank" rel="noreferrer noopener">10.1007/s10461-006-9069-7</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2006
active antiretroviral therapy
adherence
Aids and Behavior
Biomedical Social Sciences
Boarts J M
Bogart L M
CD4
Cell Count
Delahanty D L
Depression
drug-resistance
Environmental & Occupational Health
HIV
Journal Article or Conference Abstract Publication
medication
Outpatient Clinics
posttraumatic-stress-disorder
primary care
protease inhibitors
PTSD
Public
Sledjeski E M
Social Support
viral load
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
71–74
Issue
2
Volume
319
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Estrogen receptor-alpha and -beta coexist in a subpopulation of sensory neurons of female rat dorsal root ganglia.
Publisher
An entity responsible for making the resource available
Neuroscience letters
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-02
Subject
The topic of the resource
Female; Animals; Rats; Cell Count; *Sex Characteristics; Fluorescent Antibody Technique; Estrous Cycle/*physiology; Estrogen Receptor alpha; Cell Nucleus/metabolism/ultrastructure; Estrogen Receptor beta; Estrogens/*metabolism; Ganglia; Neurons; Sprague-Dawley; Receptors; Spinal/cytology/*metabolism; Genitalia; Estrogen/*metabolism; Afferent/cytology/*metabolism; Female/innervation
Creator
An entity primarily responsible for making the resource
Papka Raymond E; Storey-Workley Megan
Description
An account of the resource
Immunoreactivities for estrogen receptor-alpha (ER-alpha) and ER-beta are expressed in sensory neurons of the dorsal root ganglia (DRG). It has not been established, however, if the two receptor subtypes coexist in the same neuron. Double-staining immunohistochemical techniques were used to determine if subpopulations of neurons in the lumbosacral DRG exist based on their content of ERs. Results indicate that some neurons (approximately 17%) of the L6-S1 DRG contain ER-alpha -, some (approximately 23%) contain ER-beta - immunoreactivity and some (approximately 5%) express immunoreactivity for both subtypes of the ER. These results suggest that many sensory neurons can respond to estrogens, but estrogens may produce different morphofunctional effects in different neurons based on their expression of ER subtypes.
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Sex Characteristics
2002
Afferent/cytology/*metabolism
Animals
Cell Count
Cell Nucleus/metabolism/ultrastructure
Estrogen Receptor alpha
Estrogen Receptor beta
Estrogen/*metabolism
Estrogens/*metabolism
Estrous Cycle/*physiology
Female
Female/innervation
Fluorescent Antibody Technique
Ganglia
Genitalia
Neurons
Neuroscience letters
Papka Raymond E
Rats
Receptors
Spinal/cytology/*metabolism
Sprague-Dawley
Storey-Workley Megan
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
2419–2432
Issue
11
Volume
36
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Hormonal regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7).
Publisher
An entity responsible for making the resource available
Journal of lipid research
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-11
Subject
The topic of the resource
Animals; Rats; Gene Expression Regulation; Cell Count; Transfection; Base Sequence; Molecular Sequence Data; Phorbol Esters/pharmacology; Cholesterol 7-alpha-Hydroxylase/*genetics; Luciferases/genetics; Cyclic AMP/pharmacology; Hormones/*pharmacology; Second Messenger Systems/physiology; Cultured; Genetic; Tumor Cells; Cloning; Molecular; *Promoter Regions; *Transcription; Enzymologic/*physiology
Creator
An entity primarily responsible for making the resource
Crestani M; Stroup D; Chiang J Y
Description
An account of the resource
The transcriptional regulation of the rat cholesterol 7 alpha-hydroxylase gene (CYP7) by hormones and signal transduction pathways was studied by transient transfection assay of the promoter activity. HepG2 cells were transfected with deletion mutants of the CYP7 upstream region linked to the luciferase reporter gene. The transcription of CYP7/luciferase chimeric genes was higher in confluent than in subconfluent cultures of HepG2 cells. Glucocorticoid receptors, in the presence of dexamethasone, up-regulated the CYP7 gene through two regions located between -3262 and -2803, and between -344 and -222, respectively. Thyroid hormones did not have any effect on the promoter activity. Insulin inhibited the promoter activity through sequences located between -344 and -222, and abolished the stimulation by dexamethasone. Hence, the insulin effect was dominant over that of glucocorticoids. Treatment of transfected HepG2 cells with phorbol
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Promoter Regions
*Transcription
1995
Animals
Base Sequence
Cell Count
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cloning
Crestani M
Cultured
Cyclic AMP/pharmacology
Department of Integrative Medical Sciences
Enzymologic/*physiology
Gene Expression Regulation
Genetic
Hormones/*pharmacology
Journal of lipid research
Luciferases/genetics
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Phorbol Esters/pharmacology
Rats
Second Messenger Systems/physiology
Stroup D
Transfection
Tumor Cells
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/physiolgenomics.2000.2.3.129" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/physiolgenomics.2000.2.3.129</a>
Pages
129–136
Issue
3
Volume
2
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Inactivation of one copy of the mouse neurotrophin-3 gene induces cardiac sympathetic deficits.
Publisher
An entity responsible for making the resource available
Physiological genomics
Date
A point or period of time associated with an event in the lifecycle of the resource
2000
2000-04
Subject
The topic of the resource
*Gene Dosage; Aging/metabolism; Animals; Axons/metabolism; Body Weight/genetics; Cell Count; Coronary Vessels/innervation; Heart Rate/genetics; Heart/*innervation; Heterozygote; Homozygote; In Situ Nick-End Labeling; Knockout; Mice; Muscle Tonus/genetics; Mutant Strains; Myocardium/cytology/*metabolism; Neurotrophin 3/deficiency/*genetics; Norepinephrine/metabolism; Organ Size/genetics; Stellate Ganglion/cytology; Sympathetic Nervous System/cytology/*growth & development/metabolism; Tyrosine 3-Monooxygenase/metabolism
Creator
An entity primarily responsible for making the resource
Story G M; DiCarlo S E; Rodenbaugh D W; Dluzen D E; Kucera J; Maron M B; Walro J M
Description
An account of the resource
Whether two copies of the neurotrophin-3 (NT3) gene are necessary for proper development of cardiac sympathetic innervation was investigated in mice carrying a targeted inactivation of the NT3 gene. Heterozygous (+/-) and null (-/-) mutant mice had fewer stellate ganglion neurons than did wild-type (+/+) mice at postnatal day 0 (P0 or birth), and this deficit was maintained between adult (P60) +/- and +/+ mice. The sympathetic innervation of the heart matured postnatally in +/+ and +/- mice. Tyrosine hydroxylase (TH)-positive axons were restricted largely to the epicardium at P0, were concentrated around large blood vessels in the myocardium at P21, and were present among cardiac myocytes at P60. Cardiac norepinephrine (NE) concentrations paralleled the growth of the sympathetic axons into the heart. NE concentrations were equivalent among +/+, +/-, and -/- mice at birth, but differences between +/- and +/+ mice increased with age. Adult +/- mice also exhibited lower resting heart rates and sympathetic tonus than +/+ mice. Thus deletion of one copy of the NT3 gene translates into anatomical, biochemical, and functional deficits in cardiac sympathetic innervation of postnatal mice, thereby indicating a gene-dosage effect for the NT3 gene.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/physiolgenomics.2000.2.3.129" target="_blank" rel="noreferrer noopener">10.1152/physiolgenomics.2000.2.3.129</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Dosage
2000
Aging/metabolism
Animals
Axons/metabolism
Body Weight/genetics
Cell Count
Coronary Vessels/innervation
DiCarlo S E
Dluzen D E
Heart Rate/genetics
Heart/*innervation
Heterozygote
Homozygote
In Situ Nick-End Labeling
Knockout
Kucera J
Maron M B
Mice
Muscle Tonus/genetics
Mutant Strains
Myocardium/cytology/*metabolism
Neurotrophin 3/deficiency/*genetics
Norepinephrine/metabolism
Organ Size/genetics
Physiological genomics
Rodenbaugh D W
Stellate Ganglion/cytology
Story G M
Sympathetic Nervous System/cytology/*growth & development/metabolism
Tyrosine 3-Monooxygenase/metabolism
Walro J M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/jn.01152.2004" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/jn.01152.2004</a>
Pages
3294–3312
Issue
6
Volume
93
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Roles of inhibition in creating complex auditory responses in the inferior colliculus: facilitated combination-sensitive neurons.
Publisher
An entity responsible for making the resource available
Journal of neurophysiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-06
Subject
The topic of the resource
Acoustic Stimulation/methods; Action Potentials/*physiology; Animals; Auditory Pathways/physiology; Bicuculline/pharmacology; Cell Count; Drug Interactions; GABA Antagonists/pharmacology; Glycine Agents/pharmacology; Inferior Colliculi/*cytology; Iontophoresis/methods; Models; Neural Inhibition/drug effects/*physiology; Neurological; Neurons/classification/drug effects/*physiology/radiation effects; Otters; Reaction Time/*physiology/radiation effects; Regression Analysis; Strychnine/pharmacology; Time Factors; Wakefulness/physiology
Creator
An entity primarily responsible for making the resource
Nataraj Kiran; Wenstrup Jeffrey J
Description
An account of the resource
We studied roles of inhibition on temporally sensitive facilitation in combination-sensitive neurons from the mustached bat's inferior colliculus (IC). In these integrative neurons, excitatory responses to best frequency (BF) tones are enhanced by much lower frequency signals presented in a specific temporal relationship. Most facilitated neurons (76%) showed inhibition at delays earlier than or later than the delays causing facilitation. The timing of inhibition at earlier delays was closely related to the best delay of facilitation, but the inhibition had little influence on the duration or strength of the facilitatory interaction. Local iontophoretic application of antagonists to receptors for glycine (strychnine, STRY) and gamma-aminobutyric acid (GABA) (bicuculline, BIC) showed that STRY abolished facilitation in 96% of tested units, but BIC eliminated facilitation in only 28%. This suggests that facilitatory interactions are created in IC and reveals a differential role for these neurotransmitters. The facilitation may be created by coincidence of a postinhibitory rebound excitation activated by the low-frequency signal with the BF-evoked excitation. Unlike facilitation, inhibition at earlier delays was not eliminated by application of antagonists, suggesting an origin in lower brain stem nuclei. However, inhibition at delays later than facilitation, like facilitation itself, appears to originate within IC and to be more dependent on glycinergic than GABAergic mechanisms. Facilitatory and inhibitory interactions displayed by these combination-sensitive neurons encode information within sonar echoes and social vocalizations. The results indicate that these complex response properties arise through a series of neural interactions in the auditory brain stem and midbrain.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/jn.01152.2004" target="_blank" rel="noreferrer noopener">10.1152/jn.01152.2004</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2005
Acoustic Stimulation/methods
Action Potentials/*physiology
Animals
Auditory Pathways/physiology
Bicuculline/pharmacology
Cell Count
College of Anatomy & Neurobiology
Department of Anatomy & Neurobiology
Drug Interactions
GABA Antagonists/pharmacology
Glycine Agents/pharmacology
Inferior Colliculi/*cytology
Iontophoresis/methods
Journal of neurophysiology
Models
Nataraj Kiran
NEOMED College of Medicine
Neural Inhibition/drug effects/*physiology
Neurological
Neurons/classification/drug effects/*physiology/radiation effects
Otters
Reaction Time/*physiology/radiation effects
Regression Analysis
Strychnine/pharmacology
Time Factors
Wakefulness/physiology
Wenstrup Jeffrey J
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.neuro.2017.03.008" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.neuro.2017.03.008</a>
Pages
99–106
Volume
60
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Single low doses of MPTP decrease tyrosine hydroxylase expression in the absence of overt neuron loss.
Publisher
An entity responsible for making the resource available
Neurotoxicology
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-05
Subject
The topic of the resource
1-Methyl-4-phenyl-1; 2; 3; 6-tetrahydropyridine/*administration & dosage; Animals; Catecholamines/metabolism; Cell Count; Corpus Striatum/*drug effects/metabolism; Dopamine; Dopamine transporter; Dopaminergic Neurons/*drug effects/metabolism; Inbred C57BL; Male; Mice; MPTP; MPTP Poisoning; Parkinsonian Disorders/*metabolism/*pathology; Stereology; Substantia Nigra/*drug effects/metabolism; Tyrosine 3-Monooxygenase/*metabolism; Tyrosine hydroxylase; Vesicular monoamine transporter
Creator
An entity primarily responsible for making the resource
Alam Gelareh; Edler Melissa; Burchfield Shelbie L; Richardson Jason R
Description
An account of the resource
Parkinson's disease (PD) is the second most common age-related neurodegenerative disease. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a prototypical neurotoxicant used in mice to mimic primary features of PD pathology including striatal dopamine depletion and dopamine neuron loss in the substantia nigra pars compacta (SNc). In the literature, there are several experimental paradigms involving multiple doses of MPTP that are used to elicit dopamine neuron loss. However, a recent study reported that a single low dose caused significant loss of dopamine neurons. Here, we determined the effect of a single intraperitoneal injection of one of three doses of MPTP (0.1, 2 and 20mg/kg) on dopamine neurons, labeled by tyrosine hydroxylase (TH(+)), and total neuron number (Nissl(+)) in the SNc using unbiased stereological counting. Data reveal a significant loss of neurons in the SNc (TH(+) and Nissl(+)) only in the group treated with 20mg/kg MPTP. Groups treated with lower dose of MPTP (0.1 and 2mg/kg) only showed significant loss of TH(+) neurons rather than TH(+) and Nissl(+) neurons. Striatal dopamine levels were decreased in the groups treated with 2 and 20mg/kg MPTP and striatal terminal markers including, TH and the dopamine transporter (DAT), were only decreased in the groups treated with 20mg/kg MPTP. These data demonstrate that lower doses of MPTP likely result in loss of TH expression rather than actual dopamine neuron loss in the SN. This finding reinforces the need to measure both total neuron number along with TH(+) cells in determining dopamine neuron loss.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.neuro.2017.03.008" target="_blank" rel="noreferrer noopener">10.1016/j.neuro.2017.03.008</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1-Methyl-4-phenyl-1
2
2017
3
6-tetrahydropyridine/*administration & dosage
Alam Gelareh
Animals
Burchfield Shelbie L
Catecholamines/metabolism
Cell Count
Corpus Striatum/*drug effects/metabolism
Department of Pharmaceutical Sciences
Dopamine
Dopamine transporter
Dopaminergic Neurons/*drug effects/metabolism
Edler Melissa
Inbred C57BL
Male
Mice
MPTP
MPTP Poisoning
NEOMED College of Pharmacy
Neurotoxicology
Parkinsonian Disorders/*metabolism/*pathology
Richardson Jason R
Stereology
Substantia Nigra/*drug effects/metabolism
Tyrosine 3-Monooxygenase/*metabolism
Tyrosine hydroxylase
Vesicular monoamine transporter
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0040-8166(93)90006-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0040-8166(93)90006-7</a>
Pages
527–536
Issue
4
Volume
25
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cytometric study of the female Syrian hamster gallbladder epithelium following sex steroid administration.
Publisher
An entity responsible for making the resource available
Tissue & cell
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-08
Subject
The topic of the resource
Animals; Cell Count; Cholelithiasis/*chemically induced/pathology; Cricetinae; Cytoplasm/drug effects; Drug Administration Schedule; Epithelial Cells; Epithelium/drug effects; Estrogens/*pharmacology; Female; Gallbladder/cytology/*drug effects; Mesocricetus; Progesterone/*pharmacology
Creator
An entity primarily responsible for making the resource
Adamiec-Beyga E; Karkare S; Kelly T R; Gilloteaux J
Description
An account of the resource
This report is a cytometric study of the female Syrian hamster gallbladder epithelium following 1-, 2-, and 3-month administration of female sex steroids. Nulliparous, multiparous, young, old and pregnant hamsters were used in this study. A 1 month treatment with estrogen alone significantly increases the nuclear volume of the gallbladder epithelial cells, while E + P treatment significantly affects the nuclear volume only after a 2 month treatment. On the other hand, E + P and P treatments significantly increase the cell volumes as compared to the E-treated groups, this effect is most striking following the 1 month period. Prolonged sex steroid treatment (2 and 3 month) does not appear to influence the gallbladder epithelial cell and nuclear volumes as dramatically as that observed following the 1 month treatment. The nulliparous, progesterone-treated hamsters appear to have a greater cytoplasmic volume than the multiparous group and this is substantiated by the bulging apices and the luminal cellular excrescences observed with scanning and transmission electron microscopy. These observations are similar to those reported in ovariectomized hamsters (Gilloteaux et al., 1992). Further, the gallbladder epithelial cells and nuclei of the older female hamsters demonstrate an accentuated response to a 1 month sex steroid treatment as compared to the younger hamsters for the same treatment duration. These results enable us to hypothesize that changes induced by a short term sex steroid treatment participate in the gallstone nucleation process, while longer duration of the treatments contribute to progressive enlargement and accumulation of gallbladder calculi.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0040-8166(93)90006-7" target="_blank" rel="noreferrer noopener">10.1016/0040-8166(93)90006-7</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
Adamiec-Beyga E
Animals
Cell Count
Cholelithiasis/*chemically induced/pathology
Cricetinae
Cytoplasm/drug effects
Drug Administration Schedule
Epithelial Cells
Epithelium/drug effects
Estrogens/*pharmacology
Female
Gallbladder/cytology/*drug effects
Gilloteaux J
Karkare S
Kelly T R
Mesocricetus
Progesterone/*pharmacology
Tissue & cell
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/s002210000630" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/s002210000630</a>
Pages
219–227
Issue
2
Volume
137
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Neuropathic pain in aged rats: behavioral responses and astrocytic activation.
Publisher
An entity responsible for making the resource available
Experimental brain research
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-03
Subject
The topic of the resource
Aging/*physiology; Animal; Animal/*physiology; Animals; Astrocytes/*metabolism; Behavior; Cell Count; Disease Models; Functional Laterality/physiology; Glial Fibrillary Acidic Protein/metabolism; Hyperalgesia/physiopathology; Immunohistochemistry; Inbred F344; Lumbar Vertebrae; Male; Nerve Crush/methods; Neuralgia/*metabolism/pathology/physiopathology; Pain Measurement; Peripheral Nervous System Diseases/*metabolism/pathology/physiopathology; Posterior Horn Cells/*metabolism; Rats; Thermosensing/physiology; Touch/physiology; Up-Regulation/*physiology
Creator
An entity primarily responsible for making the resource
Stuesse S L; Crisp T; McBurney D L; Schechter J B; Lovell J A; Cruce W L
Description
An account of the resource
We used the Bennett and Xie (1988) model of chronic neuropathic pain to study the effect of age on thermal and tactile sensitivity and on astrocytic activation in the dorsal horn of the spinal cord after nerve injury. Fischer 344 FBNF1 hybrid rats in three age groups, 4-6, 14-16, and 24-26 months, were studied. Rats were either unligated (day 0, control) or the left sciatic nerve was loosely ligated to cause a chronic constriction injury (CCI). CCI causes a neuropathic pain condition characterized by tactile allodynia and thermal hyperalgesia. Rats were behaviorally assessed for tactile and thermal sensitivity of their ligated and unligated hind paws up to 35 days postligation. Rats were sacrificed before or at various days postligation, and activated astrocytes were identified at the L4-L5 levels of their spinal cords by use of an antibody to glial fibrillary acid protein (GFAP). The number of GFAP-ir astrocytes in the dorsal horn of the spinal cord in the control, uninjured condition decreased with age (P \textless or = 0.001) but increased after CCI in all three age groups. After CCI, astrocytic activation in the cord was less robust in aged rats than in younger ones (P \textless or = 0.01). Not all the CCI rats displayed hyperalgesia to touch and to heat. Rats with an increased sensitivity to heat had increased levels of GFAP-ir in their cords; however, rats with decreased thermal sensitivity also displayed increased
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1007/s002210000630" target="_blank" rel="noreferrer noopener">10.1007/s002210000630</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2001
Aging/*physiology
Animal
Animal/*physiology
Animals
Astrocytes/*metabolism
Behavior
Cell Count
Crisp T
Cruce W L
Department of Anatomy & Neurobiology
Disease Models
Experimental brain research
Functional Laterality/physiology
Glial Fibrillary Acidic Protein/metabolism
Hyperalgesia/physiopathology
Immunohistochemistry
Inbred F344
Lovell J A
Lumbar Vertebrae
Male
McBurney D L
NEOMED College of Medicine
Nerve Crush/methods
Neuralgia/*metabolism/pathology/physiopathology
Pain Measurement
Peripheral Nervous System Diseases/*metabolism/pathology/physiopathology
Posterior Horn Cells/*metabolism
Rats
Schechter J B
Stuesse S L
Thermosensing/physiology
Touch/physiology
Up-Regulation/*physiology
-
Text
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URL Address
<a href="http://doi.org/10.1002/(SICI)1097-0185(199812)252:4%3C626::AID-AR13%3E3.0.CO;2-M" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/(SICI)1097-0185(199812)252:4%3C626::AID-AR13%3E3.0.CO;2-M</a>
Pages
626–636
Issue
4
Volume
252
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Title
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Quantifying rat pulmonary intravascular mononuclear phagocytes.
Publisher
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The Anatomical record
Date
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1998
1998-12
Subject
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*Pulmonary Circulation/physiology; Animals; Cell Count; Cell Separation; Collagenases/metabolism; Indoles/pharmacology; Lung/blood supply/cytology; Male; Microspheres; Organometallic Compounds/pharmacology; Phagocytes/*physiology/ultrastructure; Phagocytosis/*physiology; Polystyrenes; Rats; Sprague-Dawley
Creator
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Niehaus G D; Mehendale S R
Description
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Cells of the mononuclear phagocyte system (MPS) protect the host by clearing effete and foreign particulates from the circulation. The current study was designed to identify, quantify, harvest, and provide a partial functional characterization of the systemic host-defense cell located in the pulmonary microvasculature of the rat. Critical colloid doses of test particulates (monastral blue B [MBB] or polystyrene beads) were infused intra-arterially into anesthetized rats so that phagocytically active pulmonary intravascular phagocytes could be identified. Morphologic characterization of in situ phagocytes was performed using electron microscopy. The number of active phagocytes was then determined using tissue samples processed for light microscopy. Finally, sequential perfusion of the pulmonary vasculature with buffer, chelating agent, and collagenase allowed elution and preliminary functional characterization of the pulmonary intravascular mononuclear phagocyte (PIMP). Electron microscopy demonstrated that both mononuclear phagocytes and neutrophils contributed to pulmonary sequestration of circulating particulates. Light microscopy showed that the microvasculature of each alveolus contained 0.50+/-0.19 active mononuclear phagocytes and 0.14+/-0.12 active neutrophils. A chelation/collagenase elution technique was then used to harvest the PIMP. Histologic evaluation of the postperfusion lungs indicated that 80% of the active phagocytes were removed by the technique. In total, the elution fluids contained 2.63+/-1.04 x 10(7) cells, with 1.60+/-0.78 x 10(7), 0.49+/-0.17 x 10(7), and 0.54+/-0.26 x 10(7) of those cells being mononuclear phagocytes, neutrophils, and lymphocytes, respectively. Functionally, the mononuclear phagocyte population exhibited a spectrum of phagocytic activities, with 51.5+/-19.5% of the cells being inactive, 33.9+/-13.4% exhibiting moderate phagocytic activity, and 14.6+/-9.8% demonstrating intensive phagocytic capacity. The current study provides the first quantified demonstration that mononuclear phagocytes are primarily responsible for sequestering blood-borne foreign particulates in the pulmonary circulation of the rat. Approximately 2 x 10(7) PIMP existed in the lungs of 300 gram rats. The functionally heterogeneous mononuclear phagocytes exhibited phagocytic capacities ranging from avidly phagocytic (14.6+/-9.8%) through moderately active (33.9+/-13.4%) to inactive. The lung microvasculature's large pool of inactive mononuclear phagocytes may provide a recruitable mechanism to allow significant increases in clearance of circulating particulates. A resident pool of activatable mononuclear phagocytes might explain previous clinical observations of increased particulate localization in the lung microvasculature of septic patients.
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<a href="http://doi.org/10.1002/(SICI)1097-0185(199812)252:4%3C626::AID-AR13%3E3.0.CO;2-M" target="_blank" rel="noreferrer noopener">10.1002/(SICI)1097-0185(199812)252:4%3C626::AID-AR13%3E3.0.CO;2-M</a>
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*Pulmonary Circulation/physiology
1998
Animals
Cell Count
Cell Separation
Collagenases/metabolism
Department of Integrative Medical Sciences
Indoles/pharmacology
Lung/blood supply/cytology
Male
Mehendale S R
Microspheres
NEOMED College of Medicine
Niehaus G D
Organometallic Compounds/pharmacology
Phagocytes/*physiology/ultrastructure
Phagocytosis/*physiology
Polystyrenes
Rats
Sprague-Dawley
The Anatomical record