1
40
2
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.cbi.2008.11.015" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.cbi.2008.11.015</a>
Pages
131–144
Issue
2
Volume
179
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Resveratrol-mediated chemoprevention of diethylnitrosamine-initiated hepatocarcinogenesis: inhibition of cell proliferation and induction of apoptosis.
Publisher
An entity responsible for making the resource available
Chemico-biological interactions
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-05
Subject
The topic of the resource
Animal; Animals; Anticarcinogenic Agents/*antagonists & inhibitors/pharmacology; Antitumor; Apoptosis/*drug effects; Body Weight/drug effects; Cell Proliferation/drug effects; Diethylnitrosamine; Disease Models; Dose-Response Relationship; Drinking/drug effects; Drug; Drug Screening Assays; Eating/drug effects; Experimental/chemically induced/pathology/*prevention & control; Female; Immunohistochemistry; Liver Neoplasms; Organ Size/drug effects; Phenobarbital; Proto-Oncogene Proteins c-bcl-2/biosynthesis; Rats; Resveratrol; Sprague-Dawley; Stilbenes/*pharmacology
Creator
An entity primarily responsible for making the resource
Bishayee Anupam; Dhir Neetika
Description
An account of the resource
Hepatocellular carcinoma (HCC) is one of the most common cancers and lethal diseases. In view of the limited treatment and a grave prognosis of liver cancer, preventive control has been emphasized. Resveratrol, a polyphenol found in grape skins, peanuts, berries and red wine, has been shown to possess potent growth inhibitory effects against various human cancer cells. Although resveratrol has been found to exhibit chemopreventive actions in experimentally induced skin, breast, colon and esophagus rodent tumors, chemopreventive potential of this dietary constituent has not been explored well against experimental liver cancer. We evaluated the inhibitory effect of resveratrol using a two-stage model of rat hepatocarcinogenesis in Sprague-Dawley rats. Initiation was performed by a single intraperitoneal injection of diethylnitrosamine (DENA, 200 mg/kg), followed by promotion with phenobarbital (0.05%) in drinking water. The rats had free access to food supplemented with resveratrol equivalent to 50, 100 or 300 mg/kg body weight/day. Resveratrol treatment was started 4 weeks prior to the initiation and continued for 20 weeks. Resveratrol dose-dependently reduced the incidence, total number and multiplicity of visible hepatocyte nodules. Mean nodular volume and nodular volume as percentage of liver volume were also inhibited upon resveratrol treatment. Histopathological examination of liver tissue confirmed the protective effect of resveratrol. Immunohistochemical detection of cell proliferation and assay of apoptosis indicated a decrease in cell proliferation and increase of apoptotic cells in the livers of resveratrol-supplemented rats. Resveratrol also induced the expression of pro-apoptotic protein Bax, reduced anti-apoptotic Bcl-2 expression, with a concurrent increase in Bax/Bcl-2 ratio with respect to DENA control. The present study provides evidence, for the first time, that resveratrol exerts a significant chemopreventive effect on DENA-initiated hepatocarcinogenesis through inhibition of cell proliferation and induction of apoptosis. Resveratrol-induced apoptogenic signal during rat liver carcinogenesis may be mediated through the downregulation of Bcl-2 and upregulation of Bax expression. Due to a favorable toxicity profile, resveratrol can potentially be developed as a chemopreventive drug against human HCC.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.cbi.2008.11.015" target="_blank" rel="noreferrer noopener">10.1016/j.cbi.2008.11.015</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
Animal
Animals
Anticarcinogenic Agents/*antagonists & inhibitors/pharmacology
Antitumor
Apoptosis/*drug effects
Bishayee Anupam
Body Weight/drug effects
Cell Proliferation/drug effects
Chemico-biological interactions
Dhir Neetika
Diethylnitrosamine
Disease Models
Dose-Response Relationship
Drinking/drug effects
Drug
Drug Screening Assays
Eating/drug effects
Experimental/chemically induced/pathology/*prevention & control
Female
Immunohistochemistry
Liver Neoplasms
Organ Size/drug effects
Phenobarbital
Proto-Oncogene Proteins c-bcl-2/biosynthesis
Rats
Resveratrol
Sprague-Dawley
Stilbenes/*pharmacology
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.cbi.2017.06.025" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.cbi.2017.06.025</a>
Pages
13–23
Volume
274
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Wogonin, a natural flavonoid, intercalates with genomic DNA and exhibits protective effects in IL-1beta stimulated osteoarthritis chondrocytes.
Publisher
An entity responsible for making the resource available
Chemico-biological interactions
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-08
Subject
The topic of the resource
Apoptosis/drug effects; Binding Sites; Cells; Chondrocytes/cytology/*drug effects/metabolism; Chondroprotective effects; Cultured; Denaturation; DNA binding; DNA/chemistry/*metabolism; Flavanones/chemistry/metabolism/*pharmacology; Flavonoids/chemistry/pharmacology; Fluorescence Resonance Energy Transfer; Humans; Intercalating Agents/chemistry/metabolism/*pharmacology; Interleukin-1beta/*pharmacology; Molecular Docking Simulation; Nucleic Acid Conformation; Osteoarthritis; Osteoarthritis/metabolism/pathology; Protective Agents/chemistry/metabolism/*pharmacology; Reactive Oxygen Species/metabolism; Up-Regulation/drug effects; Wogonin
Creator
An entity primarily responsible for making the resource
Khan Nazir M; Ahmad Imran; Ansari Mohammad Y; Haqqi Tariq M
Description
An account of the resource
Wogonin has recently been shown to possess anti-inflammatory and chondroprotective properties and is of considerable interest due to its broad pharmacological activities. The present study highlights that Wogonin binds DNA and exerts chondroprotective effects in vitro. Wogonin showed strong binding with chondrocytes genomic DNA in vitro. The mode of binding of Wogonin to genomic-DNA was assessed by competing Wogonin with EtBr or DAPI, known DNA intercalator and a minor groove binder, respectively. EtBr fluorescence reduced significantly with increase in Wogonin concentration suggesting possible DNA intercalation of Wogonin. Further, in silico molecular docking of Wogonin on mammalian DNA also indicated possible intercalation of Wogonin with DNA. The denaturation and FRET studies revealed that Wogonin prevents denaturation of DNA strands and provide stability to genomic DNA against a variety of chemical denaturants. The cellular uptake study showed that Wogonin enters osteoarthritis chondrocytes and was mainly localized in the nucleus. Wogonin treatment to OA chondrocytes protects the fragmentation of genomic DNA in response to IL-1beta as evaluated by DNA ladder and TUNEL assay. Treatment of chondrocytes with Wogonin resulted in significant suppression of IL-1beta-mediated induction of ROS. Further, Wogonin exhibited protective potential through potent suppression of extrinsic and intrinsic apoptotic pathways and induction of anti-apoptotic proteins in
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.cbi.2017.06.025" target="_blank" rel="noreferrer noopener">10.1016/j.cbi.2017.06.025</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Ahmad Imran
Ansari Mohammad Y
Apoptosis/drug effects
Binding Sites
Cells
Chemico-biological interactions
Chondrocytes/cytology/*drug effects/metabolism
Chondroprotective effects
Cultured
Denaturation
Department of Anatomy & Neurobiology
DNA binding
DNA/chemistry/*metabolism
Flavanones/chemistry/metabolism/*pharmacology
Flavonoids/chemistry/pharmacology
Fluorescence Resonance Energy Transfer
Haqqi Tariq M
Humans
Intercalating Agents/chemistry/metabolism/*pharmacology
Interleukin-1beta/*pharmacology
Khan Nazir M
Molecular Docking Simulation
NEOMED College of Medicine
Nucleic Acid Conformation
Osteoarthritis
Osteoarthritis/metabolism/pathology
Protective Agents/chemistry/metabolism/*pharmacology
Reactive Oxygen Species/metabolism
Up-Regulation/drug effects
Wogonin