1
40
3
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1039/c4ra12878h" target="_blank" rel="noreferrer noopener">http://doi.org/10.1039/c4ra12878h</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
28019-28022
Issue
35
Volume
5
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Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A naturally derived dextran-peptide vector for microRNA antagomir delivery
Publisher
An entity responsible for making the resource available
Rsc Advances
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015
Subject
The topic of the resource
cells; Chemistry; gene delivery; human cancers; in-vitro; mechanism; RNA interference; therapy; vivo
Creator
An entity primarily responsible for making the resource
Tang Q; Lei X; Cao B; Sun B B; Zhang Y Q; Cheng G
Description
An account of the resource
Single stranded microRNAs and their antagomirs are unstable and polyanionic, which impedes efficient cellular uptake and reduces half-life. Therefore, effective delivery systems with low toxicity for microRNAs are urgently needed for the success of microRNA-based therapy. Here, a dextran-peptide hybrid, Dex10-R5H5(40%), was developed as a carrier to deliver microRNAs. Dex10-R5H5(40%) loaded with antagomir-149 could reduce the level of endogenous microRNA-149 by 76% and it is more effective than the commercially available transfection reagent, RNAiMAX, which leads to 67% reduction. Additionally, Dex10-R5H5(40%) exhibited no cytotoxicity to HepG2 cells. These results indicate that the dextran-peptide hybrid may be a promising delivery system for the safe and efficient microRNA-based therapy.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1039/c4ra12878h" target="_blank" rel="noreferrer noopener">10.1039/c4ra12878h</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2015
Cao B
Cells
Chemistry
Cheng G
gene delivery
human cancers
in-vitro
Journal Article
Lei X
mechanism
RNA Interference
Rsc Advances
Sun B B
Tang Q
therapy
vivo
Zhang Y Q
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1021/la500905z" target="_blank" rel="noreferrer noopener">http://doi.org/10.1021/la500905z</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
5202-5208
Issue
18
Volume
30
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Dextran-Peptide Hybrid for Efficient Gene Delivery
Publisher
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Langmuir
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-05
Subject
The topic of the resource
cationic lipids; cells; Chemistry; cytotoxicity; drug delivery; in-vitro; Materials Science; nanoparticles; nonviral vector; plasmid dna; polyethylenimine; therapy
Creator
An entity primarily responsible for making the resource
Tang Q; Cao B; Lei X; Sun B B; Zhang Y Q; Cheng G
Description
An account of the resource
Gene therapy has drawn significant interest in the past two decades since it provides a promising strategy to treat both genetic disorders and acquired diseases. However, the transfer of gene therapy to clinical applications is troubled with many difficulties, since many current systems are of toxicity, low transfection efficiency and low biodegradability. To address these challenges, we developed a dextran-peptide hybrid system as a safe and efficient vector for gene therapy and investigated the structure-function-cytotoxicity relationship of this dextran-peptide hybrid system. Dextrans (Dex10, Dex20, and Dex70) with different molecular weights (10, 20 and 70 kDa) were conjugated with a cationic peptide, R5H5, at various degrees of substitution. Gene expression and cytotoxicity mediated by this delivery system were evaluated against SKOV-3 human ovarian carcinoma cells and compared to 25 kDa branched poly(ethylenimine) (PEI). The results showed that Dex10-R5H5 and Dex20-R5H5 hybrids derived from low molecular weight dextrans induced higher gene expression and lower cytotoxicity than Dex70-R5H5 hybrid from higher molecular weight dextran. The best performance on gene expression was achieved by Dex10-R5H5 at 40% substitution of R5H5, which induced greater gene expression than PEI at a low N/P ratio of S. Dex10-R5H5/DNA complexes at 40% substitution of R5H5 also showed much higher cell viability (93%) than PEI/DNA (66%) at the same N/P ratio. These results indicate that the Dex-R5H5 hybrid with the low molecular weight of dextran and the high degree of substitution of R5H5 is a very promising material for safe and efficient gene therapy.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1021/la500905z" target="_blank" rel="noreferrer noopener">10.1021/la500905z</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2014
Cao B
Cationic lipids
Cells
Chemistry
Cheng G
cytotoxicity
Drug delivery
in-vitro
Journal Article
Langmuir
Lei X
Materials Science
Nanoparticles
nonviral vector
plasmid dna
polyethylenimine
Sun B B
Tang Q
therapy
Zhang Y Q
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1371/journal.pone.0054460" target="_blank" rel="noreferrer noopener">http://doi.org/10.1371/journal.pone.0054460</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
8-8
Issue
1
Volume
8
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Cholesterol-Peptide Hybrids to Form Liposome-Like Vesicles for Gene Delivery
Publisher
An entity responsible for making the resource available
PLOS ONE
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-01
Subject
The topic of the resource
condensation; dna; histidine; nanoparticles; oligopeptides; polymers; prospects; Science & Technology - Other Topics; systems; therapy; vectors
Creator
An entity primarily responsible for making the resource
Tang Q; Cao B; Wu H Y; Cheng G
Description
An account of the resource
In this paper, four amphiphilic cholesterol-peptide conjugates (Ch-R5H5, Ch-R3H3, Ch-R5 and Ch-R5) were designed and synthesized, and their properties in gene delivery were evaluated in vitro with an aim of developing more efficient gene delivery carriers. These amphiphilic cholesterol-peptide conjugates are composed of hydrophobic cholesterol and positively charged peptides. They were able to self-assemble into micelles at low concentrations and their critical micelle concentrations in phosphate buffered saline (pH 7.4) are <= 85 mu g/mL. Amphiphilic cholesterol-peptide conjugates condensed DNA more efficiently than a hydrophilic cationic oligoarginine (R10) peptide with no hydrophobic segment. Their transfection efficiencies were at least two orders of magnitude greater than that of R10 peptide in HEK-293 cells. Moreover, the introduction of histidine residues in cholesterol-peptide conjugates led to higher gene expression efficiency compared with cholesterol-peptides without histidine (Ch-R5 and Ch-R3), and the luciferase expression level was comparable or even higher than that induced by PEI at its optimal N/P ratio. In particular, Ch-R5H5 condensed DNA into smaller nanoparticles than Ch-R3H3 at higher N/P ratios, and the minimum size of Ch-R5H5/DNA complexes was 180 nm with zeta potential of 23 mV, achieved at the N/P ratio of 30. This liposome-like vesicle may be a promising gene delivery carrier for intravenous therapy.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1371/journal.pone.0054460" target="_blank" rel="noreferrer noopener">10.1371/journal.pone.0054460</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2013
Cao B
Cheng G
condensation
DNA
histidine
Journal Article
Nanoparticles
oligopeptides
PloS one
Polymers
prospects
Science & Technology - Other Topics
systems
Tang Q
therapy
vectors
Wu H Y