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40
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Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jor.23262" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jor.23262</a>
Pages
311–320
Issue
2
Volume
35
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Harpagoside suppresses IL-6 expression in primary human osteoarthritis chondrocytes.
Publisher
An entity responsible for making the resource available
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-02
Subject
The topic of the resource
*c-FOS/AP-1; *harpagoside; *IL-6; *MMP-13; *osteoarthritis; CCAAT-Enhancer-Binding Protein-beta/metabolism; Chemokines/metabolism; Chondrocytes/*drug effects/metabolism; Drug Evaluation; Glycosides/pharmacology/*therapeutic use; Harpagophytum; Humans; Interleukin-1beta; Interleukin-6/antagonists & inhibitors/*metabolism; Matrix Metalloproteinase 13/metabolism; NF-kappa B/metabolism; Osteoarthritis/*drug therapy/metabolism; Phytotherapy; Plant Extracts/pharmacology/therapeutic use; Preclinical; Primary Cell Culture; Proto-Oncogene Proteins c-fos/metabolism; Pyrans/pharmacology/*therapeutic use; Reactive Oxygen Species/metabolism; Transcription Factor AP-1/metabolism
Creator
An entity primarily responsible for making the resource
Haseeb Abdul; Ansari Mohammad Yunus; Haqqi Tariq M
Description
An account of the resource
There is growing evidence in support of the involvement of inflammatory response in the pathogenesis of osteoarthritis (OA). Harpagoside, one of the bioactive components of Harpagophytum procumbens (Hp), has been shown to possess anti-inflammatory properties. Here we used an in vitro model of inflammation in OA to investigate the potential of harpagoside to suppress the production of inflammatory cytokines/chemokines such as IL-6 and matrix degrading proteases. We further investigated the likely targets of harpagoside in primary human OA chondrocytes. OA chondrocytes were pre-treated with harpagoside before stimulation with IL-1beta. mRNA expression profile of 92 cytokines/chemokines was determined using TaqMan Human Chemokine PCR Array. Expression levels of selected mRNAs were confirmed using TaqMan assays. Protein levels of IL-6 and MMP-13 were assayed by ELISA and immunoblotting. Total protein levels and phosphorylation of signaling proteins were determined by immunoblotting. Cellular localization of
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/jor.23262" target="_blank" rel="noreferrer noopener">10.1002/jor.23262</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*c-FOS/AP-1
*harpagoside
*IL-6
*MMP-13
*OSTEOARTHRITIS
2017
Ansari Mohammad Yunus
CCAAT-Enhancer-Binding Protein-beta/metabolism
Chemokines/metabolism
Chondrocytes/*drug effects/metabolism
Department of Anatomy & Neurobiology
Drug Evaluation
Glycosides/pharmacology/*therapeutic use
Haqqi Tariq M
Harpagophytum
Haseeb Abdul
Humans
Interleukin-1beta
Interleukin-6/antagonists & inhibitors/*metabolism
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
Matrix Metalloproteinase 13/metabolism
NEOMED College of Medicine
NF-kappa B/metabolism
Osteoarthritis/*drug therapy/metabolism
Phytotherapy
Plant Extracts/pharmacology/therapeutic use
Preclinical
Primary Cell Culture
Proto-Oncogene Proteins c-fos/metabolism
Pyrans/pharmacology/*therapeutic use
Reactive Oxygen Species/metabolism
Transcription Factor AP-1/metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1038/srep43789" target="_blank" rel="noreferrer noopener">http://doi.org/10.1038/srep43789</a>
Pages
43789–43789
Volume
7
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1beta-induced activation of AP-1 in human OA chondrocytes.
Publisher
An entity responsible for making the resource available
Scientific reports
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-03
Subject
The topic of the resource
Cells; Chemical Fractionation/methods; Chondrocytes/*drug effects/metabolism; Cultured; Cyclooxygenase 2/genetics/metabolism; Flavanones/*pharmacology; Gene Expression Regulation/drug effects; Humans; Interleukin-1beta/pharmacology; Interleukin-6/genetics/metabolism; Osteoarthritis/pathology; Plant Extracts/*pharmacology; Plant Roots/*chemistry; Protective Agents/pharmacology; Reactive Oxygen Species/metabolism; Scutellaria baicalensis/*chemistry; Transcription Factor AP-1/genetics/*metabolism
Creator
An entity primarily responsible for making the resource
Khan Nazir M; Haseeb Abdul; Ansari Mohammad Y; Haqqi Tariq M
Description
An account of the resource
Osteoarthritis (OA) is a common joint disorder with varying degrees of inflammation and sustained oxidative stress. The root extract of Scutellaria baicalensis (SBE) has been used for the treatment of inflammatory and other diseases. Here, we performed activity-guided HPLC-fractionation of SBE, identified the active ingredient(s) and investigated its chondroprotective potential. We found that the Wogonin containing fraction-4 (F4) was the most potent fraction based on its ability to inhibit ROS production and the suppression of catabolic markers including IL-6, COX-2, iNOS, MMP-3, MMP-9,
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1038/srep43789" target="_blank" rel="noreferrer noopener">10.1038/srep43789</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2017
Ansari Mohammad Y
Cells
Chemical Fractionation/methods
Chondrocytes/*drug effects/metabolism
Cultured
Cyclooxygenase 2/genetics/metabolism
Department of Anatomy & Neurobiology
Flavanones/*pharmacology
Gene Expression Regulation/drug effects
Haqqi Tariq M
Haseeb Abdul
Humans
Interleukin-1beta/pharmacology
Interleukin-6/genetics/metabolism
Khan Nazir M
NEOMED College of Medicine
Osteoarthritis/pathology
Plant Extracts/*pharmacology
Plant Roots/*chemistry
Protective Agents/pharmacology
Reactive Oxygen Species/metabolism
Scientific reports
Scutellaria baicalensis/*chemistry
Transcription Factor AP-1/genetics/*metabolism