1
40
17
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.2146/ajhp100214" target="_blank" rel="noreferrer noopener">http://doi.org/10.2146/ajhp100214</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
69-72
Issue
1
Volume
68
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Dublin Core
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Title
A name given to the resource
Stability Of Extemporaneously Prepared Acetylcysteine 1% And 10% Solutions For Treatment Of Meconium Ileus
Publisher
An entity responsible for making the resource available
American Journal of Health-System Pharmacy
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-01
Subject
The topic of the resource
Acetylcysteine; Chromatography; Color; Compounding; Concentration; cystic fibrosis; Diluents; edta; equivalent; Hydrogen ion concentration; Hydrogen sulfide; Ileus; Infant; liquid; management; Mucolytic agents; n-acetylcysteine; nonoperative treatment; obstruction; Odors; Pharmacology & Pharmacy; Sodium chloride; Stability; Storage
Creator
An entity primarily responsible for making the resource
Fohl A L; Johnson C E; Cober M P
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.2146/ajhp100214" target="_blank" rel="noreferrer noopener">10.2146/ajhp100214</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2011
Acetylcysteine
American Journal of Health-System Pharmacy
Chromatography
Cober M P
Color
compounding
Concentration
CYSTIC fibrosis
Department of Pharmacy Practice
Diluents
edta
equivalent
Fohl A L
hydrogen ion concentration
Hydrogen sulfide
Ileus
Infant
Johnson C E
Liquid
Management
Mucolytic agents
n-acetylcysteine
NEOMED College of Pharmacy
nonoperative treatment
obstruction
Odors
Pharmacology & Pharmacy
Sodium chloride
Stability
Storage
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.2146/100247" target="_blank" rel="noreferrer noopener">http://doi.org/10.2146/100247</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
843-845
Issue
9
Volume
68
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Stability of extemporaneously prepared glycopyrrolate oral suspensions
Publisher
An entity responsible for making the resource available
American Journal of Health-System Pharmacy
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-05
Subject
The topic of the resource
Color; Chromatography; Suspensions; Taste; Pharmacology & Pharmacy; liquid; Stability; Compounding; Concentration; Contamination; Glycopyrrolate; Hydrogen ion concentration; Parasympatholytic agents; sialorrhea; Storage; Vehicles
Creator
An entity primarily responsible for making the resource
Cober M P; Johnson C E; Sudekum D; Penprase K
Description
An account of the resource
Purpose. The stability of extemporaneously prepared glycopyrrolate 0.5-mg/mL suspensions was evaluated. Methods. An oral suspension of glycopyrrolate 0.5 mg/mL was prepared by thoroughly grinding 30 1-mg tablets of glycopyrrolate in a glass mortar. Thirty milliliters of Ora-Plus and 30 mL of either Ora-Sweet or Ora-Sweet SF were mixed and added to the powder to make a final volume of 60 mL. Three identical samples of the formulation were prepared and placed in 2-oz amber plastic bottles with child-resistant caps and stored at room temperature (23-25 degrees C). A 1-mL sample was withdrawn from each of the three bottles with a micropipette immediately after preparation and 7, 15, 30, 60, and 90 days afterward. After further dilution to an expected concentration of 50 mu g/mL with sample diluent, the samples were assayed in duplicate by stability-indicating high-performance liquid chromatography. The samples were visually examined for any color change and evaluated for pH on each day of analysis. Taste evaluations were performed at the beginning and end of the study. Stability was defined as the retention of at least 90% of the initial concentration. Results. At least 95% of the initial glycopyrrolate remained throughout the 90-day study period in both preparations. There were no detectable changes in color, odor, taste, and pH, and no visible microbial growth was observed in any sample. Conclusion. Extemporaneously compounded suspensions of glycopyrrolate 0.5 mg/mL in a 1:1 mixture of Ora-Plus/Ora-Sweet or Ora-Plus/Ora-Sweet SF were stable for at least 90 days when stored in amber plastic bottles at room temperature.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.2146/100247" target="_blank" rel="noreferrer noopener">10.2146/100247</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2011
American Journal of Health-System Pharmacy
Chromatography
Cober M P
Color
compounding
Concentration
Contamination
Department of Pharmacy Practice
Glycopyrrolate
hydrogen ion concentration
Johnson C E
Journal Article or Conference Abstract Publication
Liquid
NEOMED College of Pharmacy
Parasympatholytic agents
Penprase K
Pharmacology & Pharmacy
sialorrhea
Stability
Storage
Sudekum D
Suspensions
Taste
Vehicles
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
241-249
Issue
4
Volume
17
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Dublin Core
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Title
A name given to the resource
Specificity of cDNA-expressed human and rodent cytochrome P450s in the oxidative metabolism of the potent carcinogen 7,12-dimethylbenz a anthracene
Publisher
An entity responsible for making the resource available
Molecular Carcinogenesis
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-12
Subject
The topic of the resource
12-dimethylbenz(a)anthracene; 12-dimethylbenz(a)anthracene; 12-dimethylbenzanthracene metabolites; 7; binding; Biochemistry & Molecular Biology; cDNA expression; chromatography; cytochrome P450; high-performance liquid; human-tissues; hydrocarbons; liver; lung-cancer; metabolism of polycyclic aromatic; microsomes; mouse skin; Oncology; purification; rat; vaccinia virus
Creator
An entity primarily responsible for making the resource
Shou M G; Korzekwa K R; Krausz K W; Buters J T M; Grogan J; Goldfarb I; Hardwick J P; Gonzalez F J; Gelboin H V
Description
An account of the resource
7,12-Dimethylbenz[a]anthracene (DMBA), a potent carcinogen, requires metabolic activation by cytochrome P450s (P450s) to electrophilic metabolites that result in DNA modification, mutagenicity, and carcinogenicity. In this study, we used eight human forms, four rodent forms, and one rabbit form of P450 expressed from recombinant vaccinia or baculovirus vectors to define their specificity for metabolizing DMBA. Of the eight human P450s, 1A1 was the most active (specific activity = 14.7 nmol/min/nmol of P450) in total metabolism of DMBA and showed approximately 6- to 33-fold more activity than other P450s. 2B6, 2C9, and 1A2 were also capable of metabolizing DMBA (2.0-2.5 nmol/min/nmol of P450), whereas 2C8, 2E1, 3A4, and 3A5 exhibited relatively low activities. Among animal P450s, mouse 1A1 exhibited activity similar to that of human 1A1 and had 5.0- to 37-fold more activity than other rodent and rabbit P450s. In regard to enzyme regioselectivity, most human and rodent P450s predominantly formed the 8,9-diol, but human 2B6 and rat 2B1 preferentially formed the 5,6-diol. In the production of monohydroxymethyl metabolites, all the enzymes yielded more 7-hydroxymethyl-12-methylbenz[a]anthracene (7HOM12MBA) than 12-hydroxymethyl-7-methylbenz[a]anthracene (7M12HOMBA), except for human 1A1, which presented the reverse selectivity. Human liver microsomes from 10 organ donors were shown to metabolize DM BA and in most circumstances generated the meta belie profile DM BA trans-8,9-d dihydrodiol > 7HOM12MBA greater than or equal to DMBA trans-5,6-dihydrodiol greater than or equal to 7,12-dihydroxymethylbenz[a]anthracene > 7M12HOMBA > DMBA trans-3,4-dihydrodiol. Thus, the combined activity of hepatic microsomal 2C9, 1A2, and 2B6 may contribute to the metabolic activation and the metabolism of DMBA in normal human liver. (C) 1996 Wiley-Liss, Inc.
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An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g" target="_blank" rel="noreferrer noopener">10.1002/(sici)1098-2744(199612)17:4%3C241::aid-mc8%3E3.0.co;2-g</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
12-dimethylbenz(a)anthracene
12-dimethylbenzanthracene metabolites
1996
7
Binding
Biochemistry & Molecular Biology
Buters J T M
cDNA expression
Chromatography
cytochrome P450
Gelboin H V
Goldfarb I
Gonzalez F J
Grogan J
Hardwick J P
high-performance liquid
human-tissues
Hydrocarbons
Journal Article
Korzekwa K R
Krausz K W
Liver
lung-cancer
metabolism of polycyclic aromatic
Microsomes
Molecular carcinogenesis
mouse skin
oncology
purification
rat
Shou M G
vaccinia virus
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
191–208
Issue
2
Volume
1
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Natural cytotoxins in human plasma: isolation and characterization of phospholipids associated with cytotoxic lipoproteins.
Publisher
An entity responsible for making the resource available
Molecular toxicology
Date
A point or period of time associated with an event in the lifecycle of the resource
1987
1987-09
Subject
The topic of the resource
Animals; Mice; Cell Line; Chromatography; Cytotoxins/*blood; Lipoproteins/*blood; Molecular Weight; Phospholipids/*blood; Type C Phospholipases/pharmacology; Ultracentrifugation; Thin Layer
Creator
An entity primarily responsible for making the resource
Prezioso J A; Koo P H
Description
An account of the resource
Most of the heat-stable natural cytotoxins in normal human plasma were fractionated by gel filtration into two active fractions: the alpha
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1987
Animals
Cell Line
Chromatography
Cytotoxins/*blood
Koo P H
Lipoproteins/*blood
Mice
Molecular toxicology
Molecular Weight
Phospholipids/*blood
Prezioso J A
Thin Layer
Type C Phospholipases/pharmacology
Ultracentrifugation
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
19186–19191
Issue
29
Volume
266
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The expression of a catalytically active cholesterol 7 alpha-hydroxylase cytochrome P450 in Escherichia coli.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1991
1991-10
Subject
The topic of the resource
Gene Expression; Amino Acid Sequence; Base Sequence; Polymerase Chain Reaction; Liver/enzymology; Catalysis; Molecular Sequence Data; Substrate Specificity; Cholesterol/metabolism; Cholesterol 7-alpha-Hydroxylase/*genetics; DNA/genetics; Codon; Escherichia coli/*enzymology; Plasmids; Chromatography; Blotting; Western; Electrophoresis; Polyacrylamide Gel; Liquid; Microsomes
Creator
An entity primarily responsible for making the resource
Li Y C; Chiang J Y
Description
An account of the resource
We have recently cloned a full-length cDNA encoding the rat hepatic cholesterol 7 alpha-hydroxylase cytochrome P450 (P450c7) (Li, Y. C., Wang, D. P., and Chiang, J. Y. L. (1990) J. Biol. Chem. 265, 12012-12019), which catalyzes the rate-limiting reaction of bile acid synthesis in the liver. By using the polymerase chain reaction, we have designed two P450c7 cDNAs. One has the second Met codon deleted and the third Thr codon replaced with an Ala. The other lacks codons for the NH2-terminal hydrophobic sequence of amino acids 2-24 (P450c7 delta 2-24). The cDNAs were separately cloned into the expression vector pKK233-2 and transformed into Escherichia coli. After induction with isopropyl-beta-D-thiogalactopyranoside, bacteria harboring recombinant plasmids expressed a polypeptide which reacted with the antibody against cholesterol 7 alpha-hydroxylase in immunoblots. The slightly modified full-length enzyme was expressed to 0.2% of the total bacterial lysate and was located in the membrane fraction, whereas P450c7 delta 2-24 was expressed at a 10-fold higher level (2%), of which 85% was in the cytosol and the remaining associated with the membranes. We have purified P450c7 delta 2-24 which showed a typical reduced-CO difference spectrum of cytochrome P450 and reconstituted cholesterol 7 alpha-hydroxylase activity in the presence of NADPH-cytochrome P450 reductase. P450c7 delta 2-24 has a similar Km for cholesterol (24.6 microM) but a lower Vmax (0.10 nmol/min) and a lower turnover number (1.93 min-1) as compared with the enzyme isolated from rat liver microsomes. The purified P450c7 delta 2-24 has an unique hydrophilic NH2 terminus and contains monomers and dimers in equal amounts. This is the first report demonstrating that a genetically engineered cytochrome P450 enzyme lacking a typical NH2-terminal hydrophobic sequence is mainly cytosolic and catalytically active.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1991
Amino Acid Sequence
Base Sequence
Blotting
Catalysis
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cholesterol/metabolism
Chromatography
Codon
Department of Integrative Medical Sciences
DNA/genetics
Electrophoresis
Escherichia coli/*enzymology
Gene Expression
Li Y C
Liquid
Liver/enzymology
Microsomes
Molecular Sequence Data
NEOMED College of Medicine
Plasmids
Polyacrylamide Gel
Polymerase Chain Reaction
Substrate Specificity
The Journal of biological chemistry
Western
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0091-3057(88)90102-5" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0091-3057(88)90102-5</a>
Pages
795–799
Issue
3
Volume
30
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
A rapid and simple HPLC microassay for biogenic amines in discrete brain regions.
Publisher
An entity responsible for making the resource available
Pharmacology, biochemistry, and behavior
Date
A point or period of time associated with an event in the lifecycle of the resource
1988
1988-07
Subject
The topic of the resource
Male; Animals; Rats; Reference Values; Organ Specificity; *Brain Chemistry; Chromatography; Biogenic Amines/*analysis; Corpus Striatum/analysis; Indicators and Reagents; Nucleus Accumbens/analysis; Inbred Strains; High Pressure Liquid/methods
Creator
An entity primarily responsible for making the resource
Donzanti B A; Yamamoto B K
Description
An account of the resource
A rapid microassay is described for the measurement of biogenic amines using an isocratic HPLC system with electrochemical detection. Catecholamines, indoleamines and their major metabolites were extracted with 150 microliters of perchloric acid from brain tissue punches (less than 250 micrograms) using a simple one-step sample preparation method. These compounds were separated on a short (80 mm) column with 3 microns particle size packing, and electrochemically detected within a total run time of less than 6 minutes. Detection limit sensitivity was approximately 2-5 pg. This method, detailed in an easy-to-follow description, reduces assay time, minimizes the possibility for errors, maximizes efficiency, and requires only standard HPLC equipment and supplies.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0091-3057(88)90102-5" target="_blank" rel="noreferrer noopener">10.1016/0091-3057(88)90102-5</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Brain Chemistry
1988
Animals
Biogenic Amines/*analysis
Chromatography
Corpus Striatum/analysis
Donzanti B A
High Pressure Liquid/methods
Inbred Strains
Indicators and Reagents
Male
Nucleus Accumbens/analysis
Organ Specificity
Pharmacology, biochemistry, and behavior
Rats
Reference Values
Yamamoto B K
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0024-3205(88)90267-6" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0024-3205(88)90267-6</a>
Pages
913–922
Issue
11
Volume
43
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates.
Publisher
An entity responsible for making the resource available
Life sciences
Date
A point or period of time associated with an event in the lifecycle of the resource
1988
1905-06
Subject
The topic of the resource
Dialysis; Male; Animals; Rats; *Brain Chemistry; Chromatography; Electrochemistry; Corpus Striatum/analysis; Amino Acids/*analysis; Neurotransmitter Agents/*analysis; Inbred Strains; High Pressure Liquid/*methods
Creator
An entity primarily responsible for making the resource
Donzanti B A; Yamamoto B K
Description
An account of the resource
An improved, HPLC with electrochemical detection method for the isocratic separation and determination of amino acids from post-mortem brain tissue and from microdialysates of awake-behaving animals is described. Optimal conditions that maximize stability, resolution, and sensitivity were determined for the pre-column derivatization of amino acids using o-phthalaldehyde and
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0024-3205(88)90267-6" target="_blank" rel="noreferrer noopener">10.1016/0024-3205(88)90267-6</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Brain Chemistry
1988
Amino Acids/*analysis
Animals
Chromatography
Corpus Striatum/analysis
Dialysis
Donzanti B A
Electrochemistry
High Pressure Liquid/*methods
Inbred Strains
Life sciences
Male
Neurotransmitter Agents/*analysis
Rats
Yamamoto B K
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
3889–3897
Issue
7
Volume
265
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of cholesterol 7 alpha-hydroxylase in the liver. Purification of cholesterol 7 alpha-hydroxylase and the immunochemical evidence for the induction of cholesterol 7 alpha-hydroxylase by cholestyramine and circadian rhythm.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-03
Subject
The topic of the resource
Animals; Rats; Organ Specificity; Kinetics; *Circadian Rhythm; Enzyme Induction; Liver/drug effects/*enzymology; Cholesterol 7-alpha-Hydroxylase/*biosynthesis/isolation & purification/metabolism; Cholestyramine Resin/*pharmacology; Chromatography; Durapatite; Hydroxyapatites; Isoenzymes/*biosynthesis/isolation & purification; Obesity/enzymology; Polyethylene Glycols; Steroid Hydroxylases/*biosynthesis; Inbred Strains; Zucker; Microsomes; Ion Exchange
Creator
An entity primarily responsible for making the resource
Chiang J Y; Miller W F; Lin G M
Description
An account of the resource
Two cholesterol 7 alpha-hydroxylase isozymes were purified from liver microsomes of cholestyramine-treated female rats by using anion exchange high performance liquid chromatography. These two cytochrome P-450 isozymes were similar in electrophoretic mobility, immunocross-reactivity, and Vmax but differed in Km for cholesterol, turnover number, and charges. Antibody against the major isozyme was raised in rabbit. This antibody specifically inhibited microsomal cholesterol 7 alpha-hydroxylase activity. Immunoblot of microsomal polypeptides indicated that microsomal cholesterol 7 alpha-hydroxylase enzyme levels were increased in parallel with cholesterol 7 alpha-hydroxylase activity upon the treatment of rats with diet supplemented with cholestyramine. Both cholesterol 7 alpha-hydroxylase activity and enzyme levels were drastically reduced immediately after the removal of cholestyramine from the diet. Cholesterol 7 alpha-hydroxylase activity was also detected in the microsomes of kidney, heart, and lung in about 7-27% of the level found in the liver. 3-Methylcholanthrene treatment induced cholesterol 7 alpha-hydroxylase activity and enzyme level. In contrast, pregnenolone-16 alpha-carbonitrile or dexamethasone treatment greatly depressed enzyme and activity in rats. Cholesterol 7 alpha-hydroxylase enzyme level was 2-3-fold higher in liver microsomes of rats maintained under the reversed light cycle than under the normal light cycle. In genetically obese Zucker rats, cholesterol 7 alpha-hydroxylase activity and enzyme level did not respond to the change in the light cycle, however, were induced to the same levels as in the lean rats by cholestyramine treatment. This study provided the first direct evidence that the bile acid feedback regulation and circadian rhythm of microsomal cholesterol 7 alpha-hydroxylase activity involved the induction of cholesterol 7 alpha-hydroxylase enzyme level.
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Circadian Rhythm
1990
Animals
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*biosynthesis/isolation & purification/metabolism
Cholestyramine Resin/*pharmacology
Chromatography
Department of Integrative Medical Sciences
Durapatite
Enzyme Induction
Hydroxyapatites
Inbred Strains
Ion Exchange
Isoenzymes/*biosynthesis/isolation & purification
Kinetics
Lin G M
Liver/drug effects/*enzymology
Microsomes
Miller W F
NEOMED College of Medicine
Obesity/enzymology
Organ Specificity
Polyethylene Glycols
Rats
Steroid Hydroxylases/*biosynthesis
The Journal of biological chemistry
Zucker
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.2146/ajhp130118" target="_blank" rel="noreferrer noopener">http://doi.org/10.2146/ajhp130118</a>
Pages
1908–1912
Issue
21
Volume
70
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Evaluation and use of a rapid Staphylococcus aureus assay by an antimicrobial stewardship program.
Publisher
An entity responsible for making the resource available
American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-11
Subject
The topic of the resource
Humans; Time Factors; Microbial Sensitivity Tests; Sensitivity and Specificity; Prospective Studies; Hospitals; Anti-Bacterial Agents/administration & dosage/*pharmacology; Bacteriological Techniques; False Negative Reactions; Methicillin-Resistant Staphylococcus aureus/*isolation & purification; Staphylococcal Infections/*diagnosis/microbiology; Staphylococcus aureus/*isolation & purification; Cell Culture Techniques; Chromatography; Human; Funding Source; Community; Affinity; Observational Methods; Biological Assay – Methods; Methicillin-Resistant Staphylococcus Aureus – Analysis
Creator
An entity primarily responsible for making the resource
Trienski Tamara L; Barrett Heather L; Pasquale Timothy R; DiPersio Joseph R; File Thomas M Jr
Description
An account of the resource
PURPOSE: The performance of a rapid test for methicillin-resistant Staphylococcus aureus (MRSA) in a large community hospital was investigated. METHODS: A prospective observational study was conducted to evaluate an immunochromatographic assay (Alere PBP2a Culture Colony Test, Alere Scarborough, Inc.) for rapid differentiation of MRSA and methicillin-susceptible S. aureus (MSSA) strains using isolates cultured overnight on common laboratory media. S. aureus isolates cultured for 12-24 hours were tested with the assay, which detects penicillin-binding protein 2a (PBP2a) and provides results in six minutes. The test results were compared with data from standard overnight antimicrobial susceptibility testing to determine the assay's sensitivity and specificity. Changes in therapy associated with use of the rapid assay were evaluated. RESULTS: Over an 11-month period, 661 inpatient isolates from mostly nonhematologic sites were tested. There were six false-negative results, indicating assay sensitivity of 98.4%, with no false positives (specificity of 100%). Eight invalid test results were documented. During designated evaluation periods, a total of 169 patient cases involving PBP2a testing were reviewed by the hospital's antimicrobial stewardship pharmacist. In 63 of those cases (37%), changes in therapy were implemented on the day of test result posting. Interventions often involved switching patients from inappropriate to appropriate MRSA therapy or optimizing MRSA- or MSSA-targeted therapy. CONCLUSION: An assay for quickly differentiating between MRSA and MSSA was highly sensitive, highly specific, and inexpensive in actual hospital use and led to rapid prescription of appropriate antistaphylococcal therapy 24-48 hours after culture specimens were collected.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.2146/ajhp130118" target="_blank" rel="noreferrer noopener">10.2146/ajhp130118</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2013
Affinity
American journal of health-system pharmacy : AJHP : official journal of the American Society of Health-System Pharmacists
Anti-Bacterial Agents/administration & dosage/*pharmacology
Bacteriological Techniques
Barrett Heather L
Biological Assay – Methods
Cell Culture Techniques
Chromatography
Community
Department of Internal Medicine
DiPersio Joseph R
False Negative Reactions
File Thomas M Jr
Funding Source
Hospitals
Human
Humans
Methicillin-Resistant Staphylococcus Aureus – Analysis
Methicillin-Resistant Staphylococcus aureus/*isolation & purification
Microbial Sensitivity Tests
NEOMED College of Medicine
Observational Methods
Pasquale Timothy R
Prospective Studies
Sensitivity and Specificity
Staphylococcal Infections/*diagnosis/microbiology
Staphylococcus aureus/*isolation & purification
Time Factors
Trienski Tamara L
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/jappl.1995.78.4.1219" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/jappl.1995.78.4.1219</a>
Pages
1219–1224
Issue
4
Volume
78
Dublin Core
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Title
A name given to the resource
Daily spontaneous running alters behavioral and neurochemical indexes of nigrostriatal function.
Publisher
An entity responsible for making the resource available
Journal of applied physiology (Bethesda, Md. : 1985)
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-04
Subject
The topic of the resource
Animal/*physiology; Animals; Behavior; Chromatography; Circadian Rhythm; Corpus Striatum/cytology/*physiology; Dopamine/*metabolism; High Pressure Liquid; In Vitro Techniques; Male; Neuropsychological Tests; Organ Size; Perfusion; Physical Conditioning; Potassium/*metabolism; Psychomotor Performance/*physiology; Random Allocation; Rats; Sprague-Dawley
Creator
An entity primarily responsible for making the resource
Dluzen D E; Liu B; Chen C Y; DiCarlo S E
Description
An account of the resource
Behavioral and neurochemical indexes of nigrostriatal dopaminergic function were compared between sedentary control rats (n = 12) and daily spontaneous running (DSR) male rats (n = 10). Nine weeks of DSR did not significantly alter body, heart, pituitary, or testes weights. DSR and control animals did differ in performance on a sensorimotor beam walking task, with DSR rats showing significantly shorter times required to cross the beam (60 +/- 17 vs. 119 +/- 14s; P \textless 0.02) as well as fewer slips off the beam (3.0 +/- 0.8 vs 6.2 +/- 1.1; P \textless 0.05). DSR animals also engaged in significantly greater durations of social investigation than control rats (43 +/- 5 vs 25 +/- 3 s; P \textless 0.01) when tested in a social investigation memory-recognition test. Basal dopamine release rates from superfused corpus striatal tissue fragments of DSR rats were about one-half those obtained from control animals (18 +/- 5 vs. 34 +/- 6 pg.mg-1.min-1; P \textless 0.05), whereas responses of these striatal tissue fragments to a depolarizing concentration of potassium were virtually identical (45 +/- 10 vs. 47 +/- 8 pg.mg-1.min-1). These data indicate that a relatively limited intensity of DSR insufficient to alter cardiovascular function can exert substantial effects on behavioral and neurochemical indicators of nigrostriatal dopaminergic activity.
Identifier
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<a href="http://doi.org/10.1152/jappl.1995.78.4.1219" target="_blank" rel="noreferrer noopener">10.1152/jappl.1995.78.4.1219</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1995
Animal/*physiology
Animals
Behavior
Chen C Y
Chromatography
Circadian Rhythm
Corpus Striatum/cytology/*physiology
DiCarlo S E
Dluzen D E
Dopamine/*metabolism
High Pressure Liquid
In Vitro Techniques
Journal of applied physiology (Bethesda, Md. : 1985)
Liu B
Male
Neuropsychological Tests
Organ Size
Perfusion
Physical Conditioning
Potassium/*metabolism
Psychomotor Performance/*physiology
Random Allocation
Rats
Sprague-Dawley
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1093/chromsci/bmr055" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/chromsci/bmr055</a>
Pages
271–276
Issue
3
Volume
50
Dublin Core
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Title
A name given to the resource
Development and validation of a novel RP-HPLC method for the analysis of reduced glutathione.
Publisher
An entity responsible for making the resource available
Journal of chromatographic science
Date
A point or period of time associated with an event in the lifecycle of the resource
2012
2012-03
Subject
The topic of the resource
Chromatography; Glutathione/*analysis; High Pressure Liquid/*methods; Limit of Detection; Linear Models; Nanoparticles/chemistry; Pharmaceutical Preparations/chemistry; Reproducibility of Results; Reverse-Phase/*methods; Spectrophotometry; Ultraviolet
Creator
An entity primarily responsible for making the resource
Sutariya Vijaykumar; Wehrung Daniel; Geldenhuys Werner J
Description
An account of the resource
The objective of this study was the development, optimization, and validation of a novel reverse-phase high-pressure liquid chromatography (RP-HPLC) method for the quantification of reduced glutathione in pharmaceutical formulations utilizing simple UV detection. The separation utilized a C18 column at room temperature and UV absorption was measured at 215 nm. The mobile phase was an isocratic flow of a 50/50 (v/v) mixture of water (pH 7.0) and acetonitrile flowing at 1.0 mL/min. Validation of the method assessed the methods ability in seven categories: linearity, range, limit of detection, limit of quantification, accuracy, precision, and selectivity. Analysis of the system suitability showed acceptable levels of suitability in all categories. Likewise, the method displayed an acceptable degree of linearity (r(2) = 0.9994) over a concentration range of 2.5-60 microg/mL. The detection limit and quantification limit were 0.6 and 1.8 microg/mL respectively. The percent recovery of the method was 98.80-100.79%. Following validation the method was employed in the determination of glutathione in pharmaceutical formulations in the form of a conjugate and a nanoparticle. The proposed method offers a simple, accurate, and inexpensive way to quantify reduced glutathione.
Identifier
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<a href="http://doi.org/10.1093/chromsci/bmr055" target="_blank" rel="noreferrer noopener">10.1093/chromsci/bmr055</a>
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2012
Chromatography
Geldenhuys Werner J
Glutathione/*analysis
High Pressure Liquid/*methods
Journal of chromatographic science
Limit of Detection
Linear Models
Nanoparticles/chemistry
Pharmaceutical Preparations/chemistry
Reproducibility of Results
Reverse-Phase/*methods
Spectrophotometry
Sutariya Vijaykumar
Ultraviolet
Wehrung Daniel
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1021/acs.jproteome.5b00990" target="_blank" rel="noreferrer noopener">http://doi.org/10.1021/acs.jproteome.5b00990</a>
Pages
2115–2122
Issue
7
Volume
15
Dublin Core
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Title
A name given to the resource
Gaussian Process Modeling of Protein Turnover.
Publisher
An entity responsible for making the resource available
Journal of proteome research
Date
A point or period of time associated with an event in the lifecycle of the resource
2016
2016-07
Subject
The topic of the resource
*Brain Chemistry; *dynamic proteome; *Gaussian process; *mass spectrometry; *Ornstein-Uhlenbeck process; *protein degradation rate constant; *protein turnover rate constant; *stable isotope labeling; *stochastic differential equation for protein turnover rate constant; Animals; Chromatography; Data Interpretation; Isotope Labeling; Kinetics; Liquid; Liver/*chemistry; Mass Spectrometry; Mice; Normal Distribution; Proteins/*metabolism; Proteome/*metabolism; Proteomics/methods; Statistical; Stochastic Processes
Creator
An entity primarily responsible for making the resource
Rahman Mahbubur; Previs Stephen F; Kasumov Takhar; Sadygov Rovshan G
Description
An account of the resource
We describe a stochastic model to compute in vivo protein turnover rate constants from stable-isotope labeling and high-throughput liquid chromatography-mass spectrometry experiments. We show that the often-used one- and two-compartment nonstochastic models allow explicit solutions from the corresponding stochastic differential equations. The resulting stochastic process is a Gaussian processes with Ornstein-Uhlenbeck covariance matrix. We applied the stochastic model to a large-scale data set from (15)N labeling and compared its performance metrics with those of the nonstochastic curve fitting. The comparison showed that for more than 99% of proteins, the stochastic model produced better fits to the experimental data (based on residual sum of squares). The model was used for extracting protein-decay rate constants from mouse brain (slow turnover) and liver (fast turnover) samples. We found that the most affected (compared to two-exponent curve fitting) results were those for liver proteins. The ratio of the median of degradation rate constants of liver proteins to those of brain proteins increased 4-fold in stochastic modeling compared to the two-exponent fitting. Stochastic modeling predicted stronger differences of protein turnover processes between mouse liver and brain than previously estimated. The model is independent of the labeling isotope. To show this, we also applied the model to protein turnover studied in induced heart failure in rats, in which metabolic labeling was achieved by administering heavy water. No changes in the model were necessary for adapting to heavy-water labeling. The approach has been implemented in a freely available R code.
Identifier
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<a href="http://doi.org/10.1021/acs.jproteome.5b00990" target="_blank" rel="noreferrer noopener">10.1021/acs.jproteome.5b00990</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Brain Chemistry
*dynamic proteome
*Gaussian process
*Mass spectrometry
*Ornstein-Uhlenbeck process
*protein degradation rate constant
*protein turnover rate constant
*stable isotope labeling
*stochastic differential equation for protein turnover rate constant
2016
Animals
Chromatography
Data Interpretation
Department of Pharmaceutical Sciences
Isotope Labeling
Journal of proteome research
Kasumov Takhar
Kinetics
Liquid
Liver/*chemistry
Mass spectrometry
Mice
NEOMED College of Pharmacy
Normal Distribution
Previs Stephen F
Proteins/*metabolism
Proteome/*metabolism
Proteomics/methods
Rahman Mahbubur
Sadygov Rovshan G
Statistical
Stochastic Processes
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0361-9230(96)00216-x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0361-9230(96)00216-x</a>
Pages
95–98
Issue
2
Volume
42
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The effects of aging on spinal neurochemistry in the rat.
Publisher
An entity responsible for making the resource available
Brain research bulletin
Date
A point or period of time associated with an event in the lifecycle of the resource
1997
1905-6
Subject
The topic of the resource
Aging/*metabolism; Animals; Chromatography; Dopamine/metabolism; Electrochemistry/methods; High Pressure Liquid; Inbred F344; Male; Norepinephrine/metabolism; Rats; Serotonin/metabolism; Spinal Cord/*metabolism
Creator
An entity primarily responsible for making the resource
Ko M L; King M A; Gordon T L; Crisp T
Description
An account of the resource
The purpose of this study was to investigate how the aging process alters the basal levels of serotonin, norepinephrine, dopamine, and their respective metabolites in the spinal cord using high-performance liquid chromatography and electrochemical detection. Young, mature and aged male Fischer 344 rats (5-6,
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0361-9230(96)00216-x" target="_blank" rel="noreferrer noopener">10.1016/s0361-9230(96)00216-x</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1997
Aging/*metabolism
Animals
Brain research bulletin
Chromatography
Crisp T
Dopamine/metabolism
Electrochemistry/methods
Gordon T L
High Pressure Liquid
Inbred F344
King M A
Ko M L
Male
Norepinephrine/metabolism
Rats
Serotonin/metabolism
Spinal Cord/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.neuroscience.2008.04.051" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.neuroscience.2008.04.051</a>
Pages
1488–1496
Issue
4
Volume
154
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Differences in reserpine-induced striatal dopamine output and content between female and male mice: implications for sex differences in vesicular monoamine transporter 2 function.
Publisher
An entity responsible for making the resource available
Neuroscience
Date
A point or period of time associated with an event in the lifecycle of the resource
2008
2008-07
Subject
The topic of the resource
*Sex Characteristics; Adrenergic Uptake Inhibitors/*pharmacology; Animals; Chromatography; Corpus Striatum/drug effects/*metabolism; Dopamine/*metabolism; Female; High Pressure Liquid; Male; Mice; Orchiectomy; Ovariectomy; Reserpine/*pharmacology; Vesicular Monoamine Transport Proteins/*metabolism
Creator
An entity primarily responsible for making the resource
Dluzen D E; Bhatt S; McDermott J L
Description
An account of the resource
In this report a series of six in vitro experiments in which reserpine-evoked dopamine output and two in vivo experiments in which the effects of reserpine injections upon dopamine content from striatal tissue of female and male mice were performed as a means to assess possible sex differences in vesicular monoamine transporter 2 (VMAT2) function. Significantly greater amounts of dopamine were obtained from striatal tissue of female mice in response to either a brief (experiment 1) or continuous (experiment 2) infusion of reserpine. Similarly, reserpine-evoked dopamine output from striatal tissue of gonadectomized females was significantly greater that that of gonadectomized males (experiment 3). When reserpine-evoked dopamine responses were compared directly between intact versus gonadectomized females (experiment 4) or males (experiment 5) no statistically significant differences were obtained. Finally, comparisons of gonadectomized females treated or not with estrogen revealed no statistically significant differences in reserpine-evoked dopamine output (experiment 6). Injections of reserpine produced significantly greater depletions of striatal dopamine content within intact female versus male mice (experiment 7). Dopamine contents of gonadectomized females treated or not with estrogen did not differ following treatment with reserpine, but were significantly greater than that of gonadectomized males (experiment 8). Taken together, these results show that female striatal tissue is more responsive to reserpine-evoked dopamine output, and this sex difference appears to be estrogen independent. Similarly, the dopamine depleting effects of reserpine are greater in intact female mice, however, gonadectomy reverses this effect in an estrogen independent manner. The data suggest that female mice may have a greater amount/activity of VMAT2 function as revealed by the increased responsiveness to the VMAT2 blocking drug, reserpine. Such differences in VMAT2 function may be related to the gender differences observed in conditions like Parkinson's disease and drug addiction.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.neuroscience.2008.04.051" target="_blank" rel="noreferrer noopener">10.1016/j.neuroscience.2008.04.051</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Sex Characteristics
2008
Adrenergic Uptake Inhibitors/*pharmacology
Animals
Bhatt S
Chromatography
Corpus Striatum/drug effects/*metabolism
Dluzen D E
Dopamine/*metabolism
Female
High Pressure Liquid
Male
McDermott J L
Mice
Neuroscience
Orchiectomy
Ovariectomy
Reserpine/*pharmacology
Vesicular Monoamine Transport Proteins/*metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.jchromb.2014.05.048" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.jchromb.2014.05.048</a>
Pages
83–89
Volume
963
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Development and validation of an LC-MS/MS method for determination of the L-type voltage-gated calcium channel/NMDA receptor antagonist NGP1-01 in mouse serum.
Publisher
An entity responsible for making the resource available
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-07
Subject
The topic of the resource
Animals; Bridged-Ring Compounds/*blood; Calcium Channel Blockers/*blood; Chromatography; LC-MS/MS; Limit of Detection; Liquid/*methods; Mice; Mouse serum; Multifunctional drug; N-Methyl-D-Aspartate/*antagonists & inhibitors; Neuroprotective agent; NGP1-01; Pentacycloundecylamine; Receptors; Reproducibility of Results; Tandem Mass Spectrometry/*methods
Creator
An entity primarily responsible for making the resource
Jogiraju Harini; Zhou Xiang; Gobburi Ashta Lakshmi Prasad; Pedada Kiran K; Geldenhuys Werner J; Van der Schyf Cornelis J; Crish Samuel D; Anderson David J
Description
An account of the resource
NGP1-01 (8-benzylamino-8,11-oxapentacyclo[5.4.0.0(2,6).0(3,10).0(5,9)]undecane) is a heterocyclic cage compound with multifunctional calcium channel blocking activity that has been demonstrated to be neuroprotective in several neurodegenerative models. A sensitive internal standard LC-MS/MS method was developed and validated to quantify NGP1-01 in mouse serum. The internal standard (IS) was
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.jchromb.2014.05.048" target="_blank" rel="noreferrer noopener">10.1016/j.jchromb.2014.05.048</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2014
Anderson David J
Animals
Bridged-Ring Compounds/*blood
Calcium Channel Blockers/*blood
Chromatography
Crish Samuel D
Department of Pharmaceutical Sciences
Geldenhuys Werner J
Gobburi Ashta Lakshmi Prasad
Jogiraju Harini
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
LC-MS/MS
Limit of Detection
Liquid/*methods
Mice
Mouse serum
Multifunctional drug
N-Methyl-D-Aspartate/*antagonists & inhibitors
NEOMED College of Pharmacy
Neuroprotective agent
NGP1-01
Pedada Kiran K
Pentacycloundecylamine
Receptors
Reproducibility of Results
Tandem Mass Spectrometry/*methods
Van der Schyf Cornelis J
Zhou Xiang
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.jchromb.2013.11.048" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.jchromb.2013.11.048</a>
Pages
141–146
Volume
945-946
Dublin Core
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Title
A name given to the resource
A quantitative LC-MS/MS method for determination of thiazolidinedione mitoNEET ligand NL-1 in mouse serum suitable for pharmacokinetic studies.
Publisher
An entity responsible for making the resource available
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Date
A point or period of time associated with an event in the lifecycle of the resource
2014
2014-01
Subject
The topic of the resource
%CV; %RE; 1-methyl-4-phenyl-1; 2; 3; 3-thiazolidine-2; 4-dione; 5-[3; 5-[4-hydroxy-3; 5-di-tert-butyl-4-hydroxyphenyl)methyl]-1; 5-dimethyl-phenyl)methyl]thiazolidine-2; 6-tetrahydropyridine; Animals; Carry-over; Chromatography; High Pressure Liquid/*methods; internal standard; IS; LC-MS/MS; Limit of Detection; LLOQ; lower limit of quantification; Methanol; Mice; mitoNEET; Mouse serum; MPTP; MRM; multiple reaction monitoring; NL-1; NL-2; percent coefficient of variation; peroxisome proliferator activated receptor-gamma; pharmacokinetic; PK; PPAR-gamma; QC; quality control; relative error; relative matrix effect; RME; SD; standard deviation; Tandem Mass Spectrometry/*methods; thiazolidinedione; Thiazolidinedione (TZD); Thiazolidinediones/*blood; TZD
Creator
An entity primarily responsible for making the resource
Pedada Kiran K; Zhou Xiang; Jogiraju Harini; Carroll Richard T; Geldenhuys Werner J; Lin Li; Anderson David J
Description
An account of the resource
Thiazolidinedione (TZD) compounds have shown promise as antidiabetic, antibiotics, antifungal and neuroprotective agents. The mitochondrial effect of a novel mitoNEET ligand, NL-1 {5-[(3,5-di-tert-butyl-4-hydroxyphenyl)methyl]-1,3-thiazolidine-2,4-dione}, and other TZD compounds, is a newly proposed mechanism for the neuroprotective action of these TZD compounds. In this work, a sensitive LC-MS/MS assay has been developed and validated for quantification of NL-1 in mouse serum. Sample preparation involved an acetonitrile protein precipitation procedure with addition of an internal standard NL-2 {5-[(4-hydroxy-3,5-dimethyl-phenyl)methyl]thiazolidine-2,4-dione}. LC-MS/MS analysis utilized a Columbus C-18 HPLC column (2mmx50mm, 5mum). Chromatography employed a multiple step gradient program that featured a steep linear gradient (25-95% in 0.5min) of 15muM ammonium acetate (additive for eliminating carry-over) in 2% methanol mixing with increasing proportions of 100% methanol. The HPLC was interfaced to a QTrap 5500 mass spectrometer (AB Sciex) equipped with an electrospray ionization source used in a negative ionization mode. Multiple reaction monitoring (MRM) of m/z 334–\textgreater263 for NL-1 and m/z 250–\textgreater179 for NL-2 was done. The method had a linear range of at least 1-100ng/mL in serum. The intra-assay and inter-assay percent coefficient of variation (%CV) were less than 4% and accuracies (%RE) ranged from -2.7% to 2.0%. The analytical procedure gave 96-115% absolute extraction recovery of NL-1. The relative matrix effect was measured and found to be insignificant. The analyte in serum was confirmed to be stable during storage and treatment. The method is suitable for pharmacokinetic (PK) studies of the parent drug NL-1 based on the preliminary serum results from dosed NL-1 mouse studies.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.jchromb.2013.11.048" target="_blank" rel="noreferrer noopener">10.1016/j.jchromb.2013.11.048</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
%CV
%RE
1-Methyl-4-phenyl-1
2
2014
3
3-thiazolidine-2
4-dione
5-[3
5-[4-hydroxy-3
5-di-tert-butyl-4-hydroxyphenyl)methyl]-1
5-dimethyl-phenyl)methyl]thiazolidine-2
6-tetrahydropyridine
Anderson David J
Animals
Carroll Richard T
Carry-over
Chromatography
Department of Internal Medicine
Geldenhuys Werner J
High Pressure Liquid/*methods
internal standard
IS
Jogiraju Harini
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
LC-MS/MS
Limit of Detection
Lin Li
LLOQ
lower limit of quantification
Methanol
Mice
mitoNEET
Mouse serum
MPTP
MRM
multiple reaction monitoring
NEOMED College of Medicine
NL-1
NL-2
Pedada Kiran K
percent coefficient of variation
peroxisome proliferator activated receptor-gamma
pharmacokinetic
PK
PPAR-gamma
QC
Quality Control
relative error
relative matrix effect
RME
SD
standard deviation
Tandem Mass Spectrometry/*methods
thiazolidinedione
Thiazolidinedione (TZD)
Thiazolidinediones/*blood
TZD
Zhou Xiang
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1007/bf01250672" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf01250672</a>
Pages
197–207
Issue
3
Volume
89
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
L-dopa infusion mode differentially affects corpus striatal dopamine efflux in the presence of reserpine.
Publisher
An entity responsible for making the resource available
Journal of neural transmission. General section
Date
A point or period of time associated with an event in the lifecycle of the resource
1992
1905-6
Subject
The topic of the resource
Amphetamine/pharmacology; Animals; Chromatography; Corpus Striatum/*drug effects/metabolism; Dopamine/*metabolism; High Pressure Liquid; Infusions; Intravenous; Levodopa/*pharmacology; Male; Potassium/pharmacology; Rats; Reserpine/*pharmacology; Sprague-Dawley
Creator
An entity primarily responsible for making the resource
Dluzen D E; Kratko F T Jr
Description
An account of the resource
In the present experiment we tested the effects of L-DOPA upon dopamine (DA) efflux in vitro from superfused corpus striatal tissue fragments in medium containing reserpine. The purposes of this experiment were first, to evaluate the effects of differing infusion modes of L-DOPA upon DA efflux under conditions in which DA storage capacity has been diminished, and second, to compare this L-DOPA stimulated DA efflux with that of other putative DA secretagogues such as amphetamine and potassium. No differences were obtained in stimulated DA efflux between superfusions performed in the presence or absence of reserpine (10 microM) in the medium when L-DOPA (5 microM) was infused in a continuous (70 minute) mode during the superfusion. In contrast, a continuous infusion of either amphetamine (10 microM) or high potassium (30 mM) resulted in significantly greater stimulated DA efflux in superfusions performed with reserpine in the medium. In addition, when L-DOPA (5 microM) was administered for a brief
Identifier
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<a href="http://doi.org/10.1007/bf01250672" target="_blank" rel="noreferrer noopener">10.1007/bf01250672</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1992
Amphetamine/pharmacology
Animals
Chromatography
Corpus Striatum/*drug effects/metabolism
Dluzen D E
Dopamine/*metabolism
High Pressure Liquid
Infusions
Intravenous
Journal of neural transmission. General section
Kratko F T Jr
Levodopa/*pharmacology
Male
Potassium/pharmacology
Rats
Reserpine/*pharmacology
Sprague-Dawley