1
40
2
-
Text
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URL Address
<a href="http://doi.org/10.1371/journal.pone.0054804" target="_blank" rel="noreferrer noopener">http://doi.org/10.1371/journal.pone.0054804</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
11-11
Issue
1
Volume
8
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Extracellular ATP and Toll-Like Receptor 2 Agonists Trigger in Human Monocytes an Activation Program That Favors T Helper 17
Publisher
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PLOS ONE
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-01
Subject
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adaptive immunity; calcium ionophore; cd14(+) monocytes; cells; growth-factor-beta; host-defense; human dendritic cells; il-12 production; in-vivo; Science & Technology - Other Topics; serum-free conditions; th17
Creator
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Paustian C; Taylor P; Johnson T; Xu M; Ramirez N; Rosenthal K S; Shu S Y; Cohen P A; Czerniecki B J; Koski G K
Description
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Strategically-paired Toll-like receptor (TLR) ligands induce a unique dendritic cell (DC) phenotype that polarizes Th1 responses. We therefore investigated pairing single TLR ligands with a non TLR-mediated danger signal to cooperatively induce distinct DC properties from cultured human monocytes. Adenosine triphosphate (ATP) and the TLR2 ligand lipoteichoic acid (LTA) selectively and synergistically induced expression of IL-23 and IL-1 beta from cultured monocytes as determined by ELISA assays. Flow cytometric analysis revealed that a sizable sub-population of treated cells acquired DC-like properties including activated surface phenotype with trans-well assays showing enhanced migration towards CCR7 ligands. Such activated cells also preferentially deviated, in an IL-23 and IL-1-dependent manner, CD4(pos) T lymphocyte responses toward the IL-22(hi), IL-17(hi)/IFN-gamma(lo) Th17 phenotype in standard in vitro allogeneic sensitization assays. Although pharmacological activation of either ionotropic or cAMP-dependent pathways acted in synergy with LTA to enhance IL-23, only inhibition of the cAMP-dependent pathway antagonized ATP-enhanced cytokine production. ATP plus atypical lipopolysaccharide from P. gingivalis (signaling through TLR2) was slightly superior to E. coli-derived LPS (TLR4 ligand) for inducing the high IL-23-secreting DC-like phenotype, but greatly inferior for inducing IL-12 p70 production when paired with IFN-gamma, a distinction reflected in activated DCs' ability to deviate lymphocytes toward Th1. Collectively, our data suggest TLR2 ligands encountered by innate immune cells in an environment with physiologically-relevant levels of extracellular ATP can induce a distinct activation state favoring IL-23- and IL-1 beta-dependent Th17 type response.
Identifier
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<a href="http://doi.org/10.1371/journal.pone.0054804" target="_blank" rel="noreferrer noopener">10.1371/journal.pone.0054804</a>
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Journal Article
2013
Adaptive Immunity
calcium ionophore
cd14(+) monocytes
Cells
Cohen P A
Czerniecki B J
growth-factor-beta
host-defense
human dendritic cells
il-12 production
in-vivo
Johnson T
Journal Article
Koski G K
Paustian C
PloS one
Ramirez N
Rosenthal K S
Science & Technology - Other Topics
serum-free conditions
Shu S Y
Taylor P
th17
Xu M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.vaccine.2010.06.043" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.vaccine.2010.06.043</a>
Pages
5533–5542
Issue
34
Volume
28
Dublin Core
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Title
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J-LEAPS vaccines initiate murine Th1 responses by activating dendritic cells.
Publisher
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Vaccine
Date
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2010
2010-08
Subject
The topic of the resource
*Antigen Presentation; Adoptive Transfer; Animals; Cells; Cultured; Dendritic Cells/*immunology; Epitopes; Female; gag Gene Products; Herpes Simplex/immunology/prevention & control; Herpesvirus 1; Human Immunodeficiency Virus/immunology; Human/immunology; Inbred A; Inbred C57BL; Interferon-gamma/immunology; Interleukin-12/immunology; Mice; T-Lymphocyte/immunology; Th1 Cells/*immunology; Viral Envelope Proteins/immunology; Viral Vaccines/*immunology
Creator
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Taylor P R; Koski G K; Paustian C C; Bailey E; Cohen P A; Moore F B-G; Zimmerman D H; Rosenthal K S
Description
An account of the resource
The Ligand Epitope Antigen Presentation System (LEAPS) converts a peptide containing a T cell epitope as small as 8 amino acids into an immunogen and directs the nature of the subsequent response. Tandem synthesis of the J peptide (a peptide from the beta-2-microglobulin) with peptides of 15 or 30 amino acids from HSV-1 or HIV made them immunogenic and promoted Th1 immune responses. Immunization of A/J or C57BL/6 mice with J-LEAPS heteroconjugates containing an epitope from the HSV-1 glycoprotein D (JgD) or an epitope from the HIV gag protein (JH) emulsified with Seppic ISA51 induced increased levels of IL-12p70 by day 3 and increased levels of interferon gamma (IFN-gamma) on days 10 and 24. Interestingly, levels of IL-10, TNF-alpha, and IL-6 did not change. Neither the H nor the gD peptides alone elicited responses and only weak responses followed immunization with the J peptide. Bone marrow (BM) cells became CD86 and CD11c positive within 48 h of treatment with JgD or JH. JH or JgD treatment promoted
Identifier
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<a href="http://doi.org/10.1016/j.vaccine.2010.06.043" target="_blank" rel="noreferrer noopener">10.1016/j.vaccine.2010.06.043</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Antigen Presentation
2010
Adoptive Transfer
Animals
Bailey E
Cells
Cohen P A
Cultured
Dendritic Cells/*immunology
Epitopes
Female
gag Gene Products
Herpes Simplex/immunology/prevention & control
Herpesvirus 1
Human Immunodeficiency Virus/immunology
Human/immunology
Inbred A
Inbred C57BL
Interferon-gamma/immunology
Interleukin-12/immunology
Koski G K
Mice
Moore F B-G
Paustian C C
Rosenthal K S
T-Lymphocyte/immunology
Taylor P R
Th1 Cells/*immunology
Vaccine
Viral Envelope Proteins/immunology
Viral Vaccines/*immunology
Zimmerman D H