1
40
1
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/excr.1998.4051" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/excr.1998.4051</a>
Pages
230–241
Issue
1
Volume
241
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Type VI collagen increases cell survival and prevents anti-beta 1 integrin-mediated apoptosis.
Publisher
An entity responsible for making the resource available
Experimental cell research
Date
A point or period of time associated with an event in the lifecycle of the resource
1998
1998-05
Subject
The topic of the resource
Animals; Antibodies; Apoptosis/*drug effects/physiology; Cell Nucleus/drug effects/metabolism; Cell Survival/drug effects/physiology; Cells; Chick Embryo; Collagen/*pharmacology; Cornea/drug effects/embryology/ultrastructure; Culture Media/chemistry/pharmacology; Cultured; DNA Fragmentation/drug effects; Extracellular Matrix/physiology; Eye/drug effects/embryology/ultrastructure; Fibroblasts/drug effects/metabolism/ultrastructure; Genetic Techniques; Integrins/immunology; Monoclonal/*drug effects/pharmacology
Creator
An entity primarily responsible for making the resource
Howell S J; Doane K J
Description
An account of the resource
Cell-matrix interactions are important in the development of the avian cornea. Type VI collagen is present within the periocular mesenchyme prior to the migration of cells into the corneal stroma and is abundant in the mature stroma. Whether the interaction of cells with type VI collagen is essential for cellular survival in the cornea is not known. In the present study, we examined the interaction of corneal cells with type VI collagen in vitro to determine if it can increase cell proliferation and decrease apoptosis. In vivo analysis demonstrated that apoptosis occurs in the periocular region during early stages of avian corneal development, but in fully mature corneas apoptosis only occurs in the corneal epithelium and not in the stroma. In vitro analysis examined the importance of beta 1 integrin interactions with type VI collagen in mature corneal fibroblasts and the precursor cells. Using an anti-beta 1 integrin blocking antibody, CSAT, integrin/matrix interactions were disrupted. Results indicated that viability of both corneal fibroblasts and periocular mesenchyme cells was greater on type VI collagen than on type I collagen or BSA-blocked glass. In addition, less apoptosis was observed for both cell types on type VI collagen when beta 1 integrin–matrix interactions were disrupted. These data indicated that these cells require intact beta 1 interactions with type I collagen and with BSA-coated glass controls to remain viable. Thus, type VI collagen may play a role in the rescue of corneal cells from anti-beta 1 integrin-induced apoptosis by increasing cell survival, probably via a non-beta 1 integrin-dependent mechanism.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/excr.1998.4051" target="_blank" rel="noreferrer noopener">10.1006/excr.1998.4051</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1998
Animals
Antibodies
Apoptosis/*drug effects/physiology
Cell Nucleus/drug effects/metabolism
Cell Survival/drug effects/physiology
Cells
Chick Embryo
Collagen/*pharmacology
Cornea/drug effects/embryology/ultrastructure
Culture Media/chemistry/pharmacology
Cultured
DNA Fragmentation/drug effects
Doane K J
Experimental cell research
Extracellular Matrix/physiology
Eye/drug effects/embryology/ultrastructure
Fibroblasts/drug effects/metabolism/ultrastructure
Genetic Techniques
Howell S J
Integrins/immunology
Monoclonal/*drug effects/pharmacology