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Text
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URL Address
<a href="http://doi.org/10.1006/bbrc.1995.1582" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1995.1582</a>
Pages
894–900
Issue
3
Volume
209
Dublin Core
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Title
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The isolation and characterization of the human cytochrome b5 gene.
Publisher
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Biochemical and biophysical research communications
Date
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1995
1995-04
Subject
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Animals; Base Sequence; Cattle; Cell Line; Cloning; Cosmids; Cultured; Cytochromes b5/biosynthesis/*genetics; DNA Primers; DNA/genetics/isolation & purification; Gene Library; Genetic; Hominidae/*genetics; Humans; Introns; Luciferases/biosynthesis; Messenger/analysis/biosynthesis; Molecular; Molecular Sequence Data; Polymerase Chain Reaction; Promoter Regions; Rabbits; RNA; Transfection; Tumor Cells
Creator
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Li X R; Giordano S J; Yoo M; Steggles A W
Description
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From a series of lambda and cosmid libraries, we isolated DNA sequences corresponding to the complete coding region for the human cytochrome b5 (CYB5) gene. The overall gene organization was the same as the bovine and rabbit CYB5 genes. One cosmid clone containing exon I plus the 5' flanking region was extensively characterized. From this clone we obtained 2000 bp of 5' flanking sequence, containing several distinctive GC rich regions, potential trans-acting factor binding sites and a 74 bp direct repeat. A series of deletion constructs were made in the pGL2 luciferase vector and then used to transfect HepG2 and K562 cells. The data obtained suggest the presence of two promotors and one silencer region in the analyzed 5' sequence.
Identifier
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<a href="http://doi.org/10.1006/bbrc.1995.1582" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1995.1582</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1995
Animals
Base Sequence
Biochemical and biophysical research communications
Cattle
Cell Line
Cloning
Cosmids
Cultured
Cytochromes b5/biosynthesis/*genetics
DNA Primers
DNA/genetics/isolation & purification
Gene Library
Genetic
Giordano S J
Hominidae/*genetics
Humans
Introns
Li X R
Luciferases/biosynthesis
Messenger/analysis/biosynthesis
Molecular
Molecular Sequence Data
Polymerase Chain Reaction
Promoter Regions
Rabbits
RNA
Steggles A W
Transfection
Tumor Cells
Yoo M