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Text
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URL Address
<a href="http://doi.org/10.1016/s0040-8166(98)80061-x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0040-8166(98)80061-x</a>
Pages
475–484
Issue
4
Volume
30
Dublin Core
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Title
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Ultrastructural nucleolar alterations induced by an ametantrone–poly r(A-U) complex.
Publisher
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Tissue & cell
Date
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1998
1998-08
Subject
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Antineoplastic Agents/*pharmacology; Carcinoma; Cell Nucleolus/*chemistry/drug effects/ultrastructure; Cultured/chemistry/drug effects/ultrastructure; DNA; Humans; Immunoelectron; Microscopy; Mitoxantrone/*analogs & derivatives/pharmacology; Neoplasm/*analysis; Neoplasm/analysis; Poly A-U/*pharmacology; Ribosomal/analysis; Ribosomal/analysis/ultrastructure; RNA; Transitional Cell; Tumor Cells; Urinary Bladder Neoplasms
Creator
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Jamison J M; Gilloteaux J; Thiry M; Authelet M; Goessens G; Summers J L
Description
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The current study has documented changes in the ultrastructure as well as in the intranucleolar distribution of rDNA and rRNA in RT4 (human transitional cell bladder carcinoma) cell nucleoli following a 3-h exposure to toxic doses of 50 microM ametantrone (AMT), 200 microM poly (adenylate-uridylate) (poly r(A-U) or an AMT/poly r(A-U) combination with an AMT/polyribonucleotide ratio of 1:4 and a poly r(A-U) concentration of 200 microM. While the main nucleolar components (fibrillar center (F), dense fibrillar component (D), granular component (G) and interstices (I) can be discerned following all treatments, the nucleoli exhibit: compaction, segregation, a decrease in the number of F, an increase in the size of remaining F, margination of intranucleolar chromatin and retention of intranucleolar pre-rRNA and rRNA. The relative abilities of the test agents to induce nucleolar compaction are AMT/poly r(A-U) \textgreater poly r(A-U) \textgreater AMT \textgreater sham-treated, while the abilities of the test agents to induce the remaining nucleolar changes are AMT/poly r(A-U) \textgreater or = AMT \textgreater poly r(A-U) \textgreater sham-treated cells. Poly r(A-U) and the induced interferon induce nucleolar compaction, while AMT produces nucleolar segregation. These results are consistent with a model in which the poly r(A-U) and/or the AMT inhibit DNA transcription and rRNA processing as well as the release of nascent preribosomes from the nucleolus.
Identifier
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<a href="http://doi.org/10.1016/s0040-8166(98)80061-x" target="_blank" rel="noreferrer noopener">10.1016/s0040-8166(98)80061-x</a>
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1998
Antineoplastic Agents/*pharmacology
Authelet M
Carcinoma
Cell Nucleolus/*chemistry/drug effects/ultrastructure
Cultured/chemistry/drug effects/ultrastructure
DNA
Gilloteaux J
Goessens G
Humans
Immunoelectron
Jamison J M
Microscopy
Mitoxantrone/*analogs & derivatives/pharmacology
Neoplasm/*analysis
Neoplasm/analysis
Poly A-U/*pharmacology
Ribosomal/analysis
Ribosomal/analysis/ultrastructure
RNA
Summers J L
Thiry M
Tissue & cell
Transitional Cell
Tumor Cells
Urinary Bladder Neoplasms