Browse Items · NEOMED Bibliography Database

A novel role of transforming growth factor beta1 in transcriptional repression of human cholesterol 7alpha-hydroxylase gene.

BACKGROUND & AIMS: Inhibition of cholesterol 7alpha-hydroxylase (CYP7A1) by bile acids and inflammatory cytokines provides an important mechanism to protect hepatocytes from bile acid toxicity during cholestasis. Transforming growth factor beta1…

Mechanism of rifampicin and pregnane X receptor inhibition of human cholesterol 7 alpha-hydroxylase gene transcription.

Bile acids, steroids, and drugs activate steroid and xenobiotic receptor pregnane X receptor (PXR; NR1I2), which induces human cytochrome P4503A4 (CYP3A4) in drug metabolism and cholesterol 7 alpha-hydroxylase (CYP7A1) in bile acid synthesis in the…

Bile acids and cytokines inhibit the human cholesterol 7 alpha-hydroxylase gene via the JNK/c-jun pathway in human liver cells.

Cholesterol 7 alpha-hydroxylase (CYP7A1) of the bile acid biosynthesis pathway is suppressed by bile acids and inflammatory cytokines. Bile acids are known to induce inflammatory cytokines to activate the mitogen-activated protein kinase/c-Jun…

The isolation and characterization of the human cytochrome b5 gene.

From a series of lambda and cosmid libraries, we isolated DNA sequences corresponding to the complete coding region for the human cytochrome b5 (CYB5) gene. The overall gene organization was the same as the bovine and rabbit CYB5 genes. One cosmid…

Serotonin-activated alpha 2-macroglobulin inhibits neurite outgrowth and survival of embryonic sensory and cerebral cortical neurons.

Methylamine-modified alpha-2-macroglobulin (MA-alpha 2M) has been recently shown to inhibit the biological activity of beta-nerve growth factor (NGF) in promoting neurite outgrowth by embryonic dorsal root ganglia in culture (Koo PH, Liebl DJ, J…

Monoamine-activated alpha 2-macroglobulin inhibits choline acetyltransferase of embryonic basal forebrain neurons and reversal of the inhibition by NGF and BDNF but not NT-3.

Monoamine-activated alpha 2-macroglobulin (alpha 2M) has recently been shown to inhibit the growth and survival of cholinergic neurons of the basal forebrain (Liebl and Koo: J Neurosci Res 35:170-182, 1993). The mechanism of this inhibitory effect is…

Tags: 1994, alpha-Macroglobulins/antagonists & inhibitors/*pharmacology, Animals, Biogenic Monoamines/*pharmacology, Brain-Derived Neurotrophic Factor, Cells, Choline O-Acetyltransferase/*antagonists & inhibitors, Cultured, Humans, Journal of neuroscience research, Koo P H, Liebl D J, Nerve Growth Factors/*pharmacology, Nerve Tissue Proteins/pharmacology, Neurons/drug effects/*metabolism, Neurotrophin 3, Prosencephalon/*cytology, Rats

Histone deacetylase inhibitor vorinostat (SAHA, MK0683) perturb miR-9-MCPIP1 axis to block IL-1beta-induced IL-6 expression in human OA chondrocytes.

AIM OF THE STUDY: High levels of IL-6 are believed to contribute to osteoarthritis (OA) pathogenesis. The expression of IL-6 is regulated post-transcriptionally by the miR-9-MCPIP-1 axis in chondrocytes. Vorinostat (SAHA) inhibits the IL-6 expression…

MicroRNA-9 promotion of interleukin-6 expression by inhibiting monocyte chemoattractant protein-induced protein 1 expression in interleukin-1beta-stimulated human chondrocytes.

OBJECTIVE: Enhanced expression of interleukin-6 (IL-6) plays an important role in the pathogenesis of osteoarthritis (OA). Monocyte chemoattractant protein-induced protein 1 (MCPIP-1) is a novel posttranscriptional regulator of IL-6 expression and is…

Tags: 2015, Academic Medical Centers, Aged, Arthritis & rheumatology (Hoboken, N.J.), Articular/drug effects/*metabolism, Cartilage, Cells, Chondrocytes – Physiology, Chondrocytes/drug effects/*metabolism, Cultured, Department of Anatomy & Neurobiology, Enzyme-Linked Immunosorbent Assay, Funding Source, Gene Knockdown Techniques, Haqqi Tariq M, Haseeb Abdul, Hip/*genetics/metabolism, Human, Humans, Immunoblotting – Utilization, Immunoprecipitation, Interleukin-1beta/pharmacology, Interleukin-6/*genetics/metabolism, Interleukins – Physiology, Makki Mohammad S, Messenger/drug effects/*metabolism, MicroRNAs/drug effects/*genetics/metabolism, Middle Aged, NEOMED College of Medicine, Ohio, Osteoarthritis, Osteoarthritis – Complications, Osteoarthritis – Physiopathology, Reverse Transcriptase Polymerase Chain Reaction, Ribonucleases/drug effects/*genetics/metabolism, RNA, T-Tests, Transcription Factors/drug effects/*genetics/metabolism

An effective and efficient method of transfecting primary human chondrocytes in suspension.

Human chondrocytes accumulate an ECM-rich matrix by secreting matrix macromolecules during monolayer culture, which makes them difficult to transfect efficiently. Here we report a non-viral based protocol to transfect the primary human chondrocytes…

Strain-dependent structural variants of herpes simplex virus type 1 ICP34.5 determine viral plaque size, efficiency of glycoprotein processing, and viral release and neuroinvasive disease potential.

The ability of certain strains of herpes simplex virus type 1 (HSV-1) to cause encephalitis or neuroinvasive disease in the mouse upon peripheral infection is dependent on a combination of activities of specific forms of viral proteins. The…

Interleukin IL-12 blocks a specific subset of the transcriptional profile responsive to UVB in epidermal keratinocytes.

Interleukin-12 (IL-12) is a proinflammatory and immunomodulatory cytokine that plays a critical role it in innate and adaptive immunity by inducing production of interferon-gamma and other cytokines. IL-12 was shown to block the ultraviolet…

Tags: *Keratinocytes/metabolism/radiation effects, *Ultraviolet Rays, 2006, Blumenberg Miroslav, Cell Culture Techniques, Cells, Cultured, Epidermal Cells, Gene Expression Regulation/drug effects, Genetic/*radiation effects, Humans, Interleukin-12/*genetics/*metabolism/pharmacology, Molecular immunology, Molenda Matthew, Mukkamala Lakshmi, Transcription

Thrombospondin-4 mediates TGF-beta-induced angiogenesis.

TGF-beta is a multifunctional cytokine affecting many cell types and implicated in tissue remodeling processes. Due to its many functions and cell-specific effects, the consequences of TGF-beta signaling are process-and stage-dependent, and it is not…

Type VI collagen induces cardiac myofibroblast differentiation: implications for postinfarction remodeling.

Cardiac fibroblast (CF) proliferation and differentiation into hypersecretory myofibroblasts can lead to excessive extracellular matrix (ECM) production and cardiac fibrosis. In turn, the ECM produced can potentially activate CFs via distinct…

The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor.

BACKGROUND: Neuroinflammation is one of the hallmarks of neurodegenerative diseases, such as Parkinson's disease (PD). Activation of glial cells, including microglia and astrocytes, is a characteristic of the inflammatory response. Glycoprotein…

The presence of extracellular matrix alters the chondrocyte response to endoplasmic reticulum stress.

The objective of this study was to test the hypothesis that extracellular matrix (ECM) would alter the endoplasmic reticulum (ER) stress response of chondrocytes. Chondrocytes were isolated from calf knees and maintained in monolayer culture or…

LPS-TLR4 Pathway Mediates Ductular Cell Expansion in Alcoholic Hepatitis.

Alcoholic hepatitis (AH) is the most severe form of alcoholic liver disease for which there are no effective therapies. Patients with AH show impaired hepatocyte proliferation, expansion of inefficient ductular cells and high lipopolysaccharide (LPS)…

Tags: 2016, Affo Silvia, Alcoholic/*immunology, Altamirano Jose, Animals, Arroyo Vicente, Bataller Ramon, Caballeria Juan, Cell Proliferation, Cells, Chen Jiegen, Collagen/genetics/metabolism, Common/*pathology, Crews Fulton, Cultured, Department of Pharmaceutical Sciences, Dumitru Raluca, Female, Gene Expression Profiling, Gene Expression Regulation, Gines Pere, Hepatic Duct, Hepatitis, Hepatocytes/*metabolism/pathology, Humans, Inbred BALB C, Keratins, Knockout, Lipopolysaccharides/metabolism, Lozano Juan Jose, Male, Matsushita Hiroshi, Mice, Middle Aged, Morales-Ibanez Oriol, NEOMED College of Pharmacy, Odena Gemma, Rautou Pierre-Emmanuel, Rodrigo-Torres Daniel, Sancho-Bru Pau, Scientific reports, Seki Ekihiro, Signal Transduction, Toll-Like Receptor 4/genetics/*metabolism, Type I/genetics/*metabolism, Valla Dominique, You Min, Zou Jian

Endothelin-mediated in vivo pressor responses following TRPV1 activation.

Transient receptor potential vanilliod 1 (TRPV1) channels have recently been postulated to play a role in the vascular complications/consequences associated with diabetes despite the fact that the mechanisms through which TRPV1 regulates vascular…

Inhibition of cardiac fibroblast proliferation and myofibroblast differentiation by resveratrol.

Cardiac fibroblasts (CFs) regulate myocardial remodeling by proliferating, differentiating, and secreting extracellular matrix proteins. Prolonged activation of CFs leads to cardiac fibrosis and reduced myocardial contractile function. Resveratrol…

Tags: 2005, 70-kDa/metabolism, American journal of physiology. Heart and circulatory physiology, Angiotensin II/pharmacology, Animals, Antioxidants/*pharmacology, Bomser Joshua A, Cell Differentiation/drug effects, Cell Division/drug effects, Cells, Cultured, Department of Integrative Medical Sciences, Drug Interactions, Epidermal Growth Factor/pharmacology, Fibroblasts/*cytology/*drug effects, Male, Meszaros J Gary, Mitogen-Activated Protein Kinase 1/metabolism, Mitogen-Activated Protein Kinase 3/metabolism, Myocardium/*cytology, Naugle Jennifer E, NEOMED College of Medicine, Olson Erik R, Protein-Serine-Threonine Kinases/metabolism, Proto-Oncogene Proteins c-akt, Proto-Oncogene Proteins/metabolism, Rats, Resveratrol, Ribosomal Protein S6 Kinases, Signal Transduction/drug effects, Sprague-Dawley, Stilbenes/*pharmacology, Vasoconstrictor Agents/pharmacology, Zhang Xiaojin

Angiotensin II-induced extracellular signal-regulated kinase 1/2 activation is mediated by protein kinase Cdelta and intracellular calcium in adult rat cardiac fibroblasts.

Angiotensin II (Ang II)-induced proliferation of cardiac fibroblasts is a major contributing factor to the pathogenesis of cardiac fibrosis. Ang II activates extracellular signal-regulated kinase (ERK) 1/2 to induce cardiac fibroblast proliferation,…

Osteoactivin (GPNMB) ectodomain protein promotes growth and invasive behavior of human lung cancer cells.

The potential application of GPNMB/OA as a therapeutic target for lung cancer will require a greater understanding of the impact of GPNMB/OA ectodomain (ECD) protein shedding into tumor tissues. Thus, in this work we characterized GPNMB/OA expression…

Immune response to nonspecific and altered tissue antigens in soft tissue allografts.

Soft tissue allografts have many uses in orthopaedic surgery, including knee ligament reconstruction, hand tendon surgery, shoulder instability, and rotator cuff reconstruction. The predictable biologic incorporation of soft tissue allografts without…

Mitochondrial oxidative stress corrupts coronary collateral growth by activating adenosine monophosphate activated kinase-alpha signaling.

OBJECTIVE: Our goal was to determine the mechanism by which mitochondrial oxidative stress impairs collateral growth in the heart. APPROACH AND RESULTS: Rats were treated with rotenone (mitochondrial complex I inhibitor that increases reactive oxygen…

Tags: 2013, AMP-Activated Protein Kinases/*metabolism, Animal, Animals, Arteriosclerosis, thrombosis, and vascular biology, Body Weight/physiology, Cells, Chen Chwen-Lih, Chen Yeong-Renn, Chilian William M, Collateral Circulation, Coronary Circulation, Coronary Vessels/cytology/*enzymology, Cultured, Department of Integrative Medical Sciences, Disease Models, Dyck Jason R B, Endothelial Cells/cytology/*enzymology, Enrick Molly, Hardwick James P, Humans, Inbred WKY, Ischemia/metabolism/pathology, Kolz Christopher, Mitochondria, Mitochondria/drug effects/*metabolism, Myocardium/enzymology/pathology, NEOMED College of Medicine, Oxidative Stress/*physiology, Pung Yuh Fen, Rats, reactive oxygen species, Rotenone/pharmacology, Sam Wai Johnn, Signal Transduction/*physiology, Stevanov Kelly, Thakker Prashanth, TOR Serine-Threonine Kinases/metabolism, Uncoupling Agents/pharmacology, Yin Liya

Mechanisms of Chinese Medicine Xinmailong's protection against heart failure in pressure-overloaded mice and cultured cardiomyocytes.

Patients with heart failure (HF) have high mortality and mobility. Xinmailong (XML) injection, a Chinese Medicine, is clinically effective in treating HF. However, the mechanism of XML's effectiveness on HF was unclear, and thus, was the target of…

Optimal reactive oxygen species concentration and p38 MAP kinase are required for coronary collateral growth.

Reactive oxygen species (ROS) are implicated in coronary collateral growth (CCG). We evaluated the requirement for ROS in human coronary artery endothelial cell (HCAEC) tube formation, CCG in vivo, and signaling (p38 MAP kinase) by which ROS may…

Tags: *Collateral Circulation/drug effects, *Coronary Circulation/drug effects, *MAP Kinase Signaling System/drug effects, 2007, Acetophenones/pharmacology, American journal of physiology. Heart and circulatory physiology, Animal, Animals, Blood Flow Velocity, Cells, Chilian William M, Coronary Vessels/surgery, Cultured, Department of Integrative Medical Sciences, Disease Models, Ditiocarb/pharmacology, Endothelial Cells/drug effects/enzymology/*metabolism, Enzyme Inhibitors/pharmacology, Humans, Imidazoles/pharmacology, Inbred WKY, Kolz Christopher, Ligation, Male, Myocardial Reperfusion Injury/enzymology/metabolism/*physiopathology, NADPH Oxidases/antagonists & inhibitors/metabolism, NEOMED College of Medicine, Neovascularization, Onium Compounds/pharmacology, Oxygenases/antagonists & inhibitors/metabolism, p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism, Physiologic, Potter Barry, Pyridines/pharmacology, Rats, Reactive Oxygen Species/*metabolism, Reed Ryan, Rocic Petra, Superoxide Dismutase/antagonists & inhibitors/metabolism, Vascular Endothelial Growth Factor A/metabolism

Leptin augments recruitment of IRF-1 and CREB to thrombospondin-1 gene promoter in vascular smooth muscle cells in vitro.

We previously reported that high pathophysiological concentrations of leptin, the adipocyte-secreted peptide, upregulate the expression of a potent proatherogenic matricellular protein, thrombospondin-1 (TSP-1), in vascular smooth muscle cells.…

Tags: *cAMP response element-binding protein, *interferon regulatory factor-1, *leptin, *thrombospondin-1, *Transcription, *vascular smooth muscle cells, 2016, American journal of physiology. Cell physiology, Binding Sites/genetics, Cells, Chromatin Immunoprecipitation/methods, Cultured, Cyclic AMP Response Element-Binding Protein/*metabolism, Department of Integrative Medical Sciences, Ganguly Rituparna, Gene Expression Regulation/genetics, Genetic/genetics, Humans, Interferon Regulatory Factor-1/*metabolism, Leptin/*metabolism, Muscle, Mutagenesis, Myocytes, NEOMED College of Medicine, Promoter Regions, Raman Priya, Response Elements/genetics, Sahu Soumyadip, Site-Directed/methods, Smooth, Smooth Muscle/*metabolism, Thrombospondin 1/*genetics/*metabolism, Transcription, Transcriptional Activation/genetics, Transfection/methods, Up-Regulation/genetics, Vascular/*metabolism

Functional outcome after anal sphincter injury and treatment with mesenchymal stem cells.

This research demonstrates the regenerative effects of mesenchymal stem cells (MSCs) on the injured anal sphincter by comparing anal sphincter pressures following intramuscular and serial intravascular MSC infusion in a rat model of anal sphincter…

Histochemical analyses of tissue-engineered human menisci.

The field of tissue engineering remains one of the least explored areas of current meniscal research but holds great promise. In this investigation, meniscal fibrochondrocytes were isolated from fresh human meniscal tissue and seeded onto synthetic…

Current challenges in dedifferentiated fat cells research.

Dedifferentiated fat cells show great promises as a novel cell source for stem cell research. It has many advantages when used for cell-based therapeutics including abundance, pluripotency, and safety. However, there are many obstacles researchers…

Tags: *adult stem cells, *Biomedical Research, *Cell Dedifferentiation, *culture purity, *dedifferentiated fat cells, *dedifferentiation, *surface marker, 2016, Adipocytes/*cytology, Animals, Cells, Cultured, Evancho-Chapman M Michelle, George Richard L, Humans, Organogenesis, Phenotype, Shah Mickey, Zhang Ge

Hyperglycemia enhances function and differentiation of adult rat cardiac fibroblasts.

Diabetes is an independent risk factor for cardiovascular disease that can eventually cause cardiomyopathy and heart failure. Cardiac fibroblasts (CF) are the critical mediators of physiological and pathological cardiac remodeling; however, the…

Effects of FGF-2 and OP-1 in vitro on donor source cartilage for auricular reconstruction tissue engineering.

OBJECTIVE: Microtia is a congenital partial or total loss of the external ear with current treatment approaches involving autologous construction from costal cartilage. Alternatively, tissue engineering provides possible use of normal or microtia…

Phosphatidylinositide 3-kinase regulates angiotensin II-induced cytosolic phospholipase A2 activity and growth in vascular smooth muscle cells.

Angiotensin (Ang) II via the AT(1) receptor acts as a mitogen in vascular smooth muscle cells (VSMC) through stimulation of multiple signaling mechanisms, including tyrosine kinases and mitogen-activated protein kinase (MAPK). In addition, cytosolic…

TRPA1 and TRPV1 contribute to propofol-mediated antagonism of U46619-induced constriction in murine coronary arteries.

BACKGROUND: Transient receptor potential (TRP) ion channels have emerged as key components contributing to vasoreactivity. Propofol, an anesthetic is associated with adverse side effects including hypotension and acute pain upon infusion. Our…

Osteoactivin inhibition of osteoclastogenesis is mediated through CD44-ERK signaling.

Osteoactivin is a heavily glycosylated protein shown to have a role in bone remodeling. Previous studies from our lab have shown that mutation in Osteoactivin enhances osteoclast differentiation but inhibits their function. To date, a classical…

Glucagon and cAMP inhibit cholesterol 7alpha-hydroxylase (CYP7A1) gene expression in human hepatocytes: discordant regulation of bile acid synthesis and gluconeogenesis.

The gene encoding cholesterol 7alpha-hydroxylase (CYP7A1) is tightly regulated to control bile acid synthesis and maintain lipid homeostasis. Recent studies in mice suggest that bile acid synthesis is regulated by the fasted-to-fed cycle, and fasting…

Hepatocyte growth factor signaling pathway inhibits cholesterol 7alpha-hydroxylase and bile acid synthesis in human hepatocytes.

UNLABELLED: Bile acid synthesis in the liver is regulated by the rate-limiting enzyme cholesterol 7alpha-hydroxylase (CYP7A1). Transcription of the CYP7A1 gene is inhibited by bile acids and cytokines. The rate of bile acid synthesis is reduced…

Tags: 2007, Bile Acids and Salts/*biosynthesis, Cells, Chiang John Y L, Cholesterol 7-alpha-Hydroxylase/*biosynthesis, Cultured, Department of Integrative Medical Sciences, Ellis Ewa, Hepatocyte Growth Factor/*metabolism, Hepatocytes/*metabolism, Hepatology (Baltimore, Md.), Humans, NEOMED College of Medicine, Signal Transduction, Song Kwang-Hoon, Strom Stephen

HNF4 and COUP-TFII interact to modulate transcription of the cholesterol 7alpha-hydroxylase gene (CYP7A1).

The gene for cholesterol 7alpha-hydroxylase (CYP7A1) contains a sequence at nt -149 to -118 that was found to play a large role in determining the overall transcriptional activity and regulation of the promoter. Hepatocyte nuclear factor 4 (HNF4) and…

Identification of a bile acid response element in the cholesterol 7 alpha-hydroxylase gene CYP7A.

The transcriptional activity of the cholesterol 7 alpha-hydroxylase gene CYP7A is repressed by bile acids. Taurine conjugates of chenodeoxycholate and deoxycholate, but not cholate and ursodeoxycholate, inhibited the CYP7A promoter/luciferase…

J-LEAPS vaccines initiate murine Th1 responses by activating dendritic cells.

The Ligand Epitope Antigen Presentation System (LEAPS) converts a peptide containing a T cell epitope as small as 8 amino acids into an immunogen and directs the nature of the subsequent response. Tandem synthesis of the J peptide (a peptide from the…

Synergistic antitumour activity of vitamins C and K3 against human prostate carcinoma cell lines.

Vitamins C, K3 (VC, VK3) and a VC/VK3 combination with a VC:VK3 ratio of 100:1 were assayed for their antitumour activity against two human prostatic carcinoma cell lines. Co-administration of the vitamins enhanced the antitumour activity 5- to…

Transcriptional regulation of human oxysterol 7 alpha-hydroxylase gene (CYP7B1) by Sp1.

Oxysterol 7 alpha-hydroxylase catalyzes hydroxylation of oxysterols and neurosterols and plays a role in the alternative bile acid synthesis pathway. This gene is widely expressed in many organs and peripheral tissues and may protect tissues from the…

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