1
40
3
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Text
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URL Address
<a href="http://doi.org/10.1016/j.abb.2008.01.018" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.abb.2008.01.018</a>
Pages
1–16
Issue
1
Volume
472
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Genomic structure and regulation of the rat hepatic CYP4F1 gene by peroxisome proliferators.
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
2008
2008-04
Subject
The topic of the resource
Animals; Cell Line; Chromosome Mapping; Cytochrome P-450 Enzyme System/*genetics; Gene Expression Regulation/drug effects/*genetics; Genetic/*genetics; Hepatocytes/drug effects/*physiology; Liver/drug effects/*physiology; Peroxisome Proliferator-Activated Receptors/*genetics; Peroxisome Proliferators/*pharmacology; Promoter Regions; Rats
Creator
An entity primarily responsible for making the resource
Donelson Ellen; Chen Liping; Zhang Xiaolan; Goswami Puja; Song Byoung J; Hardwick James P
Description
An account of the resource
The rat hepatic gene CYP4F1 encodes a fatty acid omega hydroxylase P450 that metabolizes proinflammatory eicosanoids and long-chain fatty acids. We have completely sequenced the CYP4F1 gene (Accession Nos. AF200361 and AF181083), identified multiple transcription start sites, and characterized a strong core promoter region, -760/116, induced by retinoic acids and peroxisome proliferators in rat hepatoma McA-RH7777 cells. Three peroxisome proliferator responsive elements (PPRE) bind both PPARalpha/RXRalpha and HNF4alpha. Co-transfection of McA-RH7777 cells with the -760/116 reporter construct and PPARalpha/RXRalpha or HNF4alpha showed that HNF4alpha activated while PPARalpha/RXRalpha inhibited CYP4F1 promoter activity. Treating cells with Wy14,643 reversed all initial effects, indicating co-regulation of CYP4F1 gene transcription by PPARalpha/RXRalpha and HNF4alpha. Chromatin immunoprecipitation analysis of cells treated with Wy14,643 showed association of PPARalpha/RXRalpha with the active transcription of the CYP4F1 gene while in clofibrate treated rats HNF4alpha binds during gene repression, suggesting differential regulation of the CYP4F1 gene in vivo and in cell lines.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.abb.2008.01.018" target="_blank" rel="noreferrer noopener">10.1016/j.abb.2008.01.018</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2008
Animals
Archives of biochemistry and biophysics
Cell Line
Chen Liping
Chromosome Mapping
Cytochrome P-450 Enzyme System/*genetics
Department of Integrative Medical Sciences
Donelson Ellen
Gene Expression Regulation/drug effects/*genetics
Genetic/*genetics
Goswami Puja
Hardwick James P
Hepatocytes/drug effects/*physiology
Liver/drug effects/*physiology
NEOMED College of Medicine
Peroxisome Proliferator-Activated Receptors/*genetics
Peroxisome Proliferators/*pharmacology
Promoter Regions
Rats
Song Byoung J
Zhang Xiaolan
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M105117200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M105117200</a>
Pages
41690–41699
Issue
45
Volume
276
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
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Transcriptional regulation of the human sterol 12alpha-hydroxylase gene (CYP8B1): roles of heaptocyte nuclear factor 4alpha in mediating bile acid repression.
Publisher
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The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2001
2001-11
Subject
The topic of the resource
*DNA-Binding Proteins; *Transcription; Base Sequence; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Bile Acids and Salts/*pharmacology; Binding Sites; Cholesterol 7-alpha-Hydroxylase/genetics; Cultured; Cytochrome P-450 Enzyme System/*genetics; Cytoplasmic and Nuclear/physiology; Genetic; Genetic/physiology; Hepatocyte Nuclear Factor 4; Humans; Molecular Sequence Data; Phosphoproteins/*physiology; Promoter Regions; Receptors; Repressor Proteins/*pharmacology; Steroid 12-alpha-Hydroxylase; Steroid Hydroxylases/*genetics; Transcription Factors/*physiology; Tumor Cells
Creator
An entity primarily responsible for making the resource
Zhang M; Chiang J Y
Description
An account of the resource
Sterol 12alpha-hydroxylase catalyzes the synthesis of cholic acid and controls the ratio of cholic acid over chenodeoxycholic acid in the bile. Transcription of CYP8B1 is inhibited by bile acids, cholesterol, and insulin. To study the mechanism of CYP8B1 transcription by bile acids, we have cloned and determined 3389 base pairs of the 5'-upstream nucleotide sequences of the human CYP8B1. Deletion analysis of CYP8B1/luciferase reporter activity in HepG2 cells revealed that the sequences from -57 to +300 were important for basal and liver-specific promoter activities. Hepatocyte nuclear factor 4alpha (HNF4alpha) strongly activated human CYP8B1 promoter activities, whereas cholesterol 7alpha-hydroxylase promoter factor (CPF), an NR5A2 family of nuclear receptors, had much less effect. Electrophoretic mobility shift assay identified an overlapping HNF4alpha- and CPF-binding site in the +198/+227 region. The human CYP8B1 promoter activities were strongly repressed by bile acids, and the bile acid response element was localized between +137 and +220. Site-directed mutagenesis of the HNF4alpha-binding site markedly reduced promoter activity and its response to bile acid repression. On the other hand, mutation of the
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M105117200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M105117200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA-Binding Proteins
*Transcription
2001
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
Bile Acids and Salts/*pharmacology
Binding Sites
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/genetics
Cultured
Cytochrome P-450 Enzyme System/*genetics
Cytoplasmic and Nuclear/physiology
Department of Integrative Medical Sciences
Genetic
Genetic/physiology
Hepatocyte Nuclear Factor 4
Humans
Molecular Sequence Data
NEOMED College of Medicine
Phosphoproteins/*physiology
Promoter Regions
Receptors
Repressor Proteins/*pharmacology
Steroid 12-alpha-Hydroxylase
Steroid Hydroxylases/*genetics
The Journal of biological chemistry
Transcription Factors/*physiology
Tumor Cells
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
63–73
Issue
1
Volume
1583
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
On the mechanism of bile acid inhibition of rat sterol 12alpha-hydroxylase gene (CYP8B1) transcription: roles of alpha-fetoprotein transcription factor and hepatocyte nuclear factor 4alpha.
Publisher
An entity responsible for making the resource available
Biochimica et biophysica acta
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-06
Subject
The topic of the resource
Animals; Rats; Gene Expression Regulation; Base Sequence; Cattle; Cytochrome P-450 Enzyme System/*genetics; Hepatocyte Nuclear Factor 4; *DNA-Binding Proteins; Bile Acids and Salts/*pharmacology; Steroid Hydroxylases/*genetics; alpha-Fetoproteins/genetics/*physiology; DNA; Phosphoproteins/genetics/*physiology; Steroid 12-alpha-Hydroxylase; Transcription Factors/genetics/*physiology; Sprague-Dawley; RNA; Transcription; Genetic; Promoter Regions; Messenger/genetics; Enzymologic/*drug effects/physiology; Genetic/*drug effects
Creator
An entity primarily responsible for making the resource
Yang Yizeng; Zhang Ming; Eggertsen Gosta; Chiang John Y L
Description
An account of the resource
The sterol 12alpha-hydroxylase (CYP8B1) is a key enzyme of the bile acid biosynthetic pathway. It regulates the composition of bile acids in bile, i.e. ratio between cholic acid (CA) and chenodeoxycholic acid (CDCA). In similarity with cholesterol 7alpha-hydroxylase (CYP7A1), this enzyme is subjected to a negative feedback regulation by bile acids. It has been recently reported that bile acid-activated farnesoid X receptor (FXR) induces the small heterodimer partner (SHP) that interacts with alpha-fetoprotein transcription factor (FTF) and down-regulates CYP7A1 transcription. We studied whether the same mechanism also regulated rat CYP8B1 gene transcription. Feeding rats with CDCA caused a
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*DNA-Binding Proteins
2002
alpha-Fetoproteins/genetics/*physiology
Animals
Base Sequence
Bile Acids and Salts/*pharmacology
Biochimica et biophysica acta
Cattle
Chiang John Y L
Cytochrome P-450 Enzyme System/*genetics
Department of Integrative Medical Sciences
DNA
Eggertsen Gosta
Enzymologic/*drug effects/physiology
Gene Expression Regulation
Genetic
Genetic/*drug effects
Hepatocyte Nuclear Factor 4
Messenger/genetics
NEOMED College of Medicine
Phosphoproteins/genetics/*physiology
Promoter Regions
Rats
RNA
Sprague-Dawley
Steroid 12-alpha-Hydroxylase
Steroid Hydroxylases/*genetics
Transcription
Transcription Factors/genetics/*physiology
Yang Yizeng
Zhang Ming