1
40
6
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
Pages
12012–12019
Issue
20
Volume
265
Dublin Core
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Title
A name given to the resource
Regulation of cholesterol 7 alpha-hydroxylase in the liver. Cloning, sequencing, and regulation of cholesterol 7 alpha-hydroxylase mRNA.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1990
1990-07
Subject
The topic of the resource
Female; Animals; Rats; Amino Acid Sequence; *Gene Expression Regulation; Kinetics; Base Sequence; Immunoblotting; Molecular Sequence Data; Steroid Hydroxylases/*genetics; Circadian Rhythm; DNA/genetics/isolation & purification; Restriction Mapping; Enzyme Induction; Liver/drug effects/*enzymology; Cell Fractionation; Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics/immunology; Cholestyramine Resin/pharmacology; Cytochrome P-450 Enzyme System/genetics; Epitopes/analysis; Polyribosomes/metabolism/ultrastructure; Inbred Strains; RNA; Enzymologic; Sequence Homology; Cloning; Nucleic Acid; Messenger/*genetics; Centrifugation; Density Gradient; Molecular/methods
Creator
An entity primarily responsible for making the resource
Li Y C; Wang D P; Chiang J Y
Description
An account of the resource
Monospecific antibody against purified rat liver cholesterol 7 alpha-hydroxylase cytochrome P-450 was used to screen a lambda gt11 cDNA library constructed from immuno-enriched polysomal RNA of cholestyramine-treated female rat liver. Two types of cDNA clones differing in the length of the 3'-untranslated region were identified, and DNA sequences were determined. The full length clone contains 3561 base pairs plus a long poly(A) tail. The amino acid sequence deduced from the open reading frame revealed a unique P-450 protein containing 503 amino acid residues which belonged to a new gene family designated family VII or CYP7. Southern blot hybridization experiments indicated that the minimal size of P-450 VII gene was 11 kilobase pairs (kb), and there was probably only one gene in this new family. Northern blot hybridization using specific cDNA probes revealed at least two major mRNA species of about 4.0 kb and 2.1 kb, respectively. These two mRNA species may be derived from the use of different polyadenylation signals and reverse-transcribed to two types of cDNA clones. Cholesterol 7 alpha-hydroxylase mRNAs were induced 2- to 3-fold in rat liver by cholestyramine treatment. The mRNA level was rapidly reduced upon the removal of the inducer. Similarly, cholesterol feeding induced enzyme activity, protein, and mRNA levels in the rat by 2-fold, suggesting that cholesterol is an important regulator of cholesterol 7 alpha-hydroxylase in the liver. On the other hand, dexamethasone and pregnenolone-16 alpha-carbonitrile drastically reduced the activity, protein, and mRNA levels. These experiments suggest that the induction of cholesterol 7 alpha-hydroxylase activity by cholestyramine or cholesterol and inhibition of cholesterol 7 alpha-hydroxylase activity by bile acid feedback are results of the rapid turnover of cholesterol 7 alpha-hydroxylase enzyme and mRNA levels.
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
1990
Amino Acid Sequence
Animals
Base Sequence
Cell Fractionation
Centrifugation
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/biosynthesis/*genetics/immunology
Cholestyramine Resin/pharmacology
Circadian Rhythm
Cloning
Cytochrome P-450 Enzyme System/genetics
Density Gradient
Department of Integrative Medical Sciences
DNA/genetics/isolation & purification
Enzyme Induction
Enzymologic
Epitopes/analysis
Female
Immunoblotting
Inbred Strains
Kinetics
Li Y C
Liver/drug effects/*enzymology
Messenger/*genetics
Molecular Sequence Data
Molecular/methods
NEOMED College of Medicine
Nucleic Acid
Polyribosomes/metabolism/ultrastructure
Rats
Restriction Mapping
RNA
Sequence Homology
Steroid Hydroxylases/*genetics
The Journal of biological chemistry
Wang D P
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.4081/reumatismo.2002.272" target="_blank" rel="noreferrer noopener">http://doi.org/10.4081/reumatismo.2002.272</a>
Pages
272–284
Issue
3
Volume
54
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Contributions of paleorheumatology to understanding contemporary disease.
Publisher
An entity responsible for making the resource available
Reumatismo
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-09
Subject
The topic of the resource
Humans; Animals; Immunohistochemistry; Body Temperature; Phylogeny; Osteoarthritis/pathology; Radiography; Fossils; Bone Remodeling; DNA; DNA/genetics/isolation & purification; *Paleopathology/instrumentation/methods; *Rheumatology; Bone and Bones/chemistry/diagnostic imaging/immunology; Bone Diseases/*pathology; Collagen/immunology/isolation & purification; Dinosaurs; Gout/pathology; Joint Diseases/*pathology; Mammals; Microscopy/methods; Rheumatic Diseases/pathology; Imaging; Arthritis; Three-Dimensional; Sequence Analysis; Infectious/pathology; Reactive/pathology
Creator
An entity primarily responsible for making the resource
Rothschild B
Description
An account of the resource
As paleopathology has evolved from observational speculation to analysis of testable hypotheses, so too has recognition of its contribution to vertebrate paleontology. In the presence of significant structural and density variation (between matrix and osseous structures), x-rays provide an additional perspective of osseous response to stress and disease. As film techniques are time and cost expensive, fluoroscopy has proven a valuable alternative. Radiologic techniques also allow non-invasive "sectioning" of specimens, illustrating significant internal detail. The object can be "split" on a plane and the two portions rotated to "open" the image. This three-dimensional approach now can be applied to other forms of sequential data to their facilitate 3-dimensional representation graphically or with solid representations. Antigen and microstructure may be well preserved in fossils. Molecular preservation with retention of helical structure and sensitivity to collagenase has been demonstrated in 10,000 year old collagen. Antigen has been extracted from 100 million year old bone and documented, in situ, in 11,000 year old bone. If the appropriate site in the tissue is assessed, if antigen is still present, and if the appropriate antisera is utilized, fixation of the antibody to the specimen can be detected. Minute amounts of DNA can be amplified and analyzed. Recovery of DNA from a 40,000 year old mammoth, 17,000 year old bison and from 25 million year old insects provides opportunity for cloning and independent assessment of relationships. Implications of available technology focuses direction for development of collaborative approaches.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.4081/reumatismo.2002.272" target="_blank" rel="noreferrer noopener">10.4081/reumatismo.2002.272</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Paleopathology/instrumentation/methods
*Rheumatology
2002
Animals
Arthritis
Body Temperature
Bone and Bones/chemistry/diagnostic imaging/immunology
Bone Diseases/*pathology
Bone Remodeling
Collagen/immunology/isolation & purification
Dinosaurs
DNA
DNA/genetics/isolation & purification
Fossils
Gout/pathology
Humans
Imaging
Immunohistochemistry
Infectious/pathology
Joint Diseases/*pathology
Mammals
Microscopy/methods
Osteoarthritis/pathology
Phylogeny
Radiography
Reactive/pathology
Reumatismo
Rheumatic Diseases/pathology
Rothschild B
Sequence Analysis
Three-Dimensional
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/0167-4781(93)90274-h" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/0167-4781(93)90274-h</a>
Pages
95–100
Issue
1
Volume
1172
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Differential expression of the mRNAs for the soluble and membrane-bound forms of rabbit cytochrome b5.
Publisher
An entity responsible for making the resource available
Biochimica et biophysica acta
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-02
Subject
The topic of the resource
Agar Gel; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cell Membrane/metabolism; Cytochromes b5/*genetics; Cytosol/metabolism; DNA/genetics/isolation & purification; Electrophoresis; Exons; Leukocytes/metabolism; Liver/*metabolism; Messenger/genetics/isolation & purification/*metabolism; Molecular Sequence Data; Oligodeoxyribonucleotides; Polymerase Chain Reaction/methods; Rabbits; Reticulocytes/metabolism; RNA; Southern
Creator
An entity primarily responsible for making the resource
Giordano S J; Steggles A W
Description
An account of the resource
Total RNA was extracted from a variety of rabbit tissues and reverse transcribed for use in the polymerase chain reaction technique. Using primers designed to amplify the membrane-bound liver cytochrome b5 cDNA, products of two sizes were observed. Both hybridized strongly to a radiolabelled liver cytochrome b5 probe. Sequencing confirmed that the two types of cDNA product encoded the membrane-bound and the soluble forms of b5. Messenger RNA corresponding to the soluble cytochrome was detected in the lung, gallbladder and the adrenal gland, as well as in reticulocytes and bone marrow. This was an unexpected finding since the protein has been isolated only from erythrocytes. In contrast, membrane-bound cytochrome b5 mRNA was detected in all tissues tested, suggesting that the corresponding protein is ubiquitous in tissue distribution.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/0167-4781(93)90274-h" target="_blank" rel="noreferrer noopener">10.1016/0167-4781(93)90274-h</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
Agar Gel
Amino Acid Sequence
Animals
Base Sequence
Biochimica et biophysica acta
Blotting
Cell Membrane/metabolism
Cytochromes b5/*genetics
Cytosol/metabolism
DNA/genetics/isolation & purification
Electrophoresis
Exons
Giordano S J
Leukocytes/metabolism
Liver/*metabolism
Messenger/genetics/isolation & purification/*metabolism
Molecular Sequence Data
Oligodeoxyribonucleotides
Polymerase Chain Reaction/methods
Rabbits
Reticulocytes/metabolism
RNA
Southern
Steggles A W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/geno.1993.1331" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/geno.1993.1331</a>
Pages
348–354
Issue
2
Volume
17
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The isolation and characterization of the bovine cytochrome b5 gene, and a transcribed pseudogene.
Publisher
An entity responsible for making the resource available
Genomics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-08
Subject
The topic of the resource
*Pseudogenes; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cattle/*genetics; Cytochromes b5/*genetics; DNA/genetics/isolation & purification; Exons; Gene Expression; Genetic; Genomic Library; Humans; Introns; Messenger/biosynthesis/metabolism; Molecular Sequence Data; Nucleic Acid; Nucleic Acid Conformation; Oligodeoxyribonucleotides; Rabbits; Restriction Mapping; Reticulocytes/metabolism; RNA; RNA Precursors/chemistry/metabolism; Sequence Homology; Southern; Transcription
Creator
An entity primarily responsible for making the resource
Cristiano R J; Giordano S J; Steggles A W
Description
An account of the resource
This is the first isolation and characterization of a cytochrome b5(b5) gene. The bovine b5 gene is quite large, spanning about 28 kb and contains six exons. One of these exons appears to code for a reticulocyte-specific sequence similar to that described for human and rabbit b5. All of the splicing junctions conform to the GT-AG consensus rule. The 5' flanking sequence has no obvious TATA box, two CAAT boxes, and contains several G:C-rich SpI motifs indicative of a house-keeping gene. In reticulocyte mRNA we found evidence for a transcribed b5 pseudogene, but could not detect sequences coding for the soluble form of b5. We conclude that the soluble form of b5 is derived from the membrane-bound b5 by a post-translational mechanism.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/geno.1993.1331" target="_blank" rel="noreferrer noopener">10.1006/geno.1993.1331</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Pseudogenes
1993
Amino Acid Sequence
Animals
Base Sequence
Blotting
Cattle/*genetics
Cristiano R J
Cytochromes b5/*genetics
DNA/genetics/isolation & purification
Exons
Gene Expression
Genetic
Genomic Library
Genomics
Giordano S J
Humans
Introns
Messenger/biosynthesis/metabolism
Molecular Sequence Data
Nucleic Acid
Nucleic Acid Conformation
Oligodeoxyribonucleotides
Rabbits
Restriction Mapping
Reticulocytes/metabolism
RNA
RNA Precursors/chemistry/metabolism
Sequence Homology
Southern
Steggles A W
Transcription
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/bbrc.1995.1582" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1995.1582</a>
Pages
894–900
Issue
3
Volume
209
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The isolation and characterization of the human cytochrome b5 gene.
Publisher
An entity responsible for making the resource available
Biochemical and biophysical research communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1995
1995-04
Subject
The topic of the resource
Animals; Base Sequence; Cattle; Cell Line; Cloning; Cosmids; Cultured; Cytochromes b5/biosynthesis/*genetics; DNA Primers; DNA/genetics/isolation & purification; Gene Library; Genetic; Hominidae/*genetics; Humans; Introns; Luciferases/biosynthesis; Messenger/analysis/biosynthesis; Molecular; Molecular Sequence Data; Polymerase Chain Reaction; Promoter Regions; Rabbits; RNA; Transfection; Tumor Cells
Creator
An entity primarily responsible for making the resource
Li X R; Giordano S J; Yoo M; Steggles A W
Description
An account of the resource
From a series of lambda and cosmid libraries, we isolated DNA sequences corresponding to the complete coding region for the human cytochrome b5 (CYB5) gene. The overall gene organization was the same as the bovine and rabbit CYB5 genes. One cosmid clone containing exon I plus the 5' flanking region was extensively characterized. From this clone we obtained 2000 bp of 5' flanking sequence, containing several distinctive GC rich regions, potential trans-acting factor binding sites and a 74 bp direct repeat. A series of deletion constructs were made in the pGL2 luciferase vector and then used to transfect HepG2 and K562 cells. The data obtained suggest the presence of two promotors and one silencer region in the analyzed 5' sequence.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/bbrc.1995.1582" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1995.1582</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1995
Animals
Base Sequence
Biochemical and biophysical research communications
Cattle
Cell Line
Cloning
Cosmids
Cultured
Cytochromes b5/biosynthesis/*genetics
DNA Primers
DNA/genetics/isolation & purification
Gene Library
Genetic
Giordano S J
Hominidae/*genetics
Humans
Introns
Li X R
Luciferases/biosynthesis
Messenger/analysis/biosynthesis
Molecular
Molecular Sequence Data
Polymerase Chain Reaction
Promoter Regions
Rabbits
RNA
Steggles A W
Transfection
Tumor Cells
Yoo M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/abbi.1993.1537" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/abbi.1993.1537</a>
Pages
451–460
Issue
2
Volume
306
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Genomic cloning, sequencing, and analysis of the hamster cholesterol 7 alpha-hydroxylase gene (CYP7).
Publisher
An entity responsible for making the resource available
Archives of biochemistry and biophysics
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-11
Subject
The topic of the resource
Amino Acid; Amino Acid Sequence; Animals; Base Sequence; Blotting; Cholesterol 7-alpha-Hydroxylase/*genetics; Cloning; Cricetinae; DNA/genetics/isolation & purification; Genomic Library; Humans; Liver/enzymology; Mesocricetus/*genetics; Messenger/biosynthesis/metabolism; Molecular; Molecular Sequence Data; Northern; Poly A/biosynthesis/metabolism; Rats; Restriction Mapping; RNA; Sequence Homology; Southern
Creator
An entity primarily responsible for making the resource
Crestani M; Galli G; Chiang J Y
Description
An account of the resource
Cholesterol 7 alpha-hydroxylase is the rate limiting enzyme in bile acid biosynthesis and plays an important role in cholesterol homeostasis. The Golden Syrian hamster has been used as an animal model for the study of atherosclerosis and cholesterol gallstone disease. We have screened a lambda DASH II hamster liver genomic library using a rat cDNA as a hybridization probe. A 14-kb genomic clone has been isolated and characterized by restriction mapping and Southern blot hybridization. The clone contained the full-length gene encoding cholesterol 7 alpha-hydroxylase together with an upstream sequence of approximately 5 kb. DNA sequencing and analysis of about 11 kb of the gene revealed that the hamster CYP7 gene consists of six exons and five introns, which have the same structures and sizes as predicted in the rat and human CYP7 genes. The nucleotide and deduced amino acid sequences of the hamster cholesterol 7 alpha-hydroxylase have a high sequence identity of about 90% to the rat and 82% to the human sequences. Particularly, exons 2, 5, and 6 are highly conserved among these species, thus reflecting the presence of some domains that are crucial for the activity of this unique enzyme. The putative cholesterol-binding region, an aromatic amino acid region, and the P450 heme-binding region are completely conserved. Comparison of the 250-bp 5'-flanking sequence to the corresponding region in the rat and human genes revealed a high degree of homology ranging between 71% and 82%. Next to the canonical TATA and CCAAT boxes are many consensus sequences (LF-A1, LF-B1, TGT3) for liver-specific or -enriched transcription factors (HNF4, HNF1, and HNF5, respectively) and an imperfect direct repeat of thyroid hormone responsive element (TRE), which is located between TGT3 and LF-B1. These sequence motifs are completely conserved among the rat, human, and hamster CYP7 genes. Several modified sterol regulatory element (SRE)-like sequences are located in the upstream flanking region and in the first intron. This highly conserved proximal promoter may play important roles in the transcription activity and in the regulation of the CYP7 gene by physiological agents, such as bile acids and steroid/thyroid hormones. This is the first report describing the complete nucleotide sequence and confirming the structure of a CYP7 gene.(ABSTRACT TRUNCATED AT 400 WORDS)
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/abbi.1993.1537" target="_blank" rel="noreferrer noopener">10.1006/abbi.1993.1537</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
1993
Amino Acid
Amino Acid Sequence
Animals
Archives of biochemistry and biophysics
Base Sequence
Blotting
Chiang J Y
Cholesterol 7-alpha-Hydroxylase/*genetics
Cloning
Crestani M
Cricetinae
Department of Integrative Medical Sciences
DNA/genetics/isolation & purification
Galli G
Genomic Library
Humans
Liver/enzymology
Mesocricetus/*genetics
Messenger/biosynthesis/metabolism
Molecular
Molecular Sequence Data
NEOMED College of Medicine
Northern
Poly A/biosynthesis/metabolism
Rats
Restriction Mapping
RNA
Sequence Homology
Southern