Description
1. gamma-Aminobutyric acidA (GABAA) receptor-mediated inhibition of pyramidal neuron dendrites was studied in area CA1 of the rat hippocampal slice preparation with the use of intracellular and extracellular recording and one-dimensional current source-density (CSD) analysis. 2. Electrical stimulation of Schaffer collateral/commissural fibers evoked monosynaptic excitatory postsynaptic potentials (EPSPs) and population EPSPs, which were followed by biphasic inhibitory postsynaptic potentials (IPSPs). In the presence of the excitatory amino acid receptor antagonists 6,7-dinitroquinoxaline-2,3-dione (DNQX) and D,L-2-amino-5-phosphonovalerate (APV), stimulation in stratum radiatum evoked monosynaptic fast, GABAA and late, GABAB receptor-mediated IPSPs and fast and late positive field potentials recorded in s. radiatum. 3. Fast monosynaptic IPSPs and fast positive field potentials evoked in the presence of DNQX and APV were reversibly abolished by the GABAA receptor antagonist bicuculline methiodide (BMI; 30 microM) and were not changed by the GABAB receptor antagonist
Subject
2-Amino-5-phosphonovalerate/pharmacology; Animals; Bicuculline/analogs & derivatives/pharmacology; Chlorides/pharmacology; Dendrites/drug effects/*physiology; Evoked Potentials/drug effects; GABA-A Receptor Antagonists; GABA-A/drug effects/*physiology; Hippocampus/*physiology; In Vitro Techniques; Kinetics; Mathematics; Membrane Potentials/drug effects; Models; Neurological; Neurons/drug effects/*physiology; Organophosphorus Compounds/pharmacology; Pyramidal Tracts/drug effects/*physiology; Quinoxalines/pharmacology; Rats; Receptors; Synapses/drug effects/physiology