1
40
15
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajpgi.00170.2004" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpgi.00170.2004</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
G60-G66
Issue
1
Volume
288
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Fxr-activating Ligands Inhibit Rabbit Asbt Expression Via Fxr-shp-ftf Cascade
Publisher
An entity responsible for making the resource available
American Journal of Physiology-Gastrointestinal and Liver Physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-01
Subject
The topic of the resource
bile-acid transporter; cloning; down-regulation; Gastroenterology & Hepatology; identification; ileal; messenger-rna; negative feedback-regulation; nuclear receptor; Physiology; rats; real-time
Creator
An entity primarily responsible for making the resource
Li H; Chen F; Shang Q; Pan L X; Shneider B L; Chiang J Y L; Forman B M; Ananthanarayanan M; Tint G S; Salen G; Xu G R
Description
An account of the resource
The regulation of the rabbit apical sodium-dependent bile acid transporter ( ASBT) was studied both in vivo and in vitro. New Zealand White rabbits were fed 0.5% deoxycholic acid (DCA) or SC-435, a competitive ASBT inhibitor, for 1 wk. In DCA-fed rabbits, ASBT expression was repressed, associated with activated FXR, and evidenced by increased ileal short heterodimer partner (SHP) mRNA. Feeding SC-435 to the rabbits blocked bile acid absorption, decreased SHP mRNA, and increased ASBT expression. A 1.9-kb rabbit ASBT 5'-flanking region (promoter) was cloned, and a cis-acting element for alpha-fetoprotein transcription factor (FTF) was identified (-1166/-1158). The effects of transcriptional factors and different bile acids on the rabbit ASBT promoter were studied in Caco-2 cells. FTF stimulated the rabbit ASBT promoter activity fourfold but not after the FTF binding site was deleted from the promoter. Increasing the SHP protein notably inhibited FTF-dependent trans-activation of rabbit ASBT. Adding hydrophobic bile acids deoxycholic acid, chenodeoxycholic acid, and cholic acid, activating ligands for FXR, inhibited rabbit ASBT promoter activity in Caco-2 cells, but this inhibitory effect was abolished after the FTF binding site was deleted. Ursodeoxycholic acid and ursocholic acid, nonactivating ligands for FXR, did not repress ASBT promoter activity. Thus the rabbit ASBT promoter is negative-feedback regulated by bile acids via a functional FTF binding site. Only FXR-activating ligands can downregulate rabbit ASBT expression through the regulatory cascade FXR-SHP-FTF.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajpgi.00170.2004" target="_blank" rel="noreferrer noopener">10.1152/ajpgi.00170.2004</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2005
American Journal of Physiology-Gastrointestinal and Liver Physiology
Ananthanarayanan M
bile-acid transporter
Chen F
Chiang J Y L
Cloning
Down-Regulation
Forman B M
Gastroenterology & Hepatology
identification
ileal
Journal Article or Conference Abstract Publication
Li H
messenger-rna
negative feedback-regulation
Nuclear Receptor
Pan L X
Physiology
Rats
real-time
Salen G
Shang Q
Shneider B L
Tint G S
Xu G R
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1093/rheumatology/kes363" target="_blank" rel="noreferrer noopener">http://doi.org/10.1093/rheumatology/kes363</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
998-1008
Issue
6
Volume
52
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Title
A name given to the resource
Delphinidin Inhibits Il-1 Beta-induced Activation Of Nf-kappa B By Modulating The Phosphorylation Of Irak-1(ser376) In Human Articular Chondrocytes
Publisher
An entity responsible for making the resource available
Rheumatology
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-06
Subject
The topic of the resource
chondrocytes; COX-2; delphinidin; down-regulation; factor-alpha; gene-expression; human osteoarthritis chondrocytes; IL-1 beta; in-vitro; interleukin-1; kinase; nf-kappa-b; osteoarthritis; oxidative stress; PGE(2); rheumatoid-arthritis; Rheumatology; tumor-necrosis-factor
Creator
An entity primarily responsible for making the resource
Haseeb A; Chen D X; Haqqi T M
Description
An account of the resource
Objective. In OA, there is enhanced expression of pro-inflammatory cytokines such as IL-1 beta in the affected joint. Delphinidin, an anthocyanidin found in pigmented fruits and vegetables, has been shown to possess anti-inflammatory and antioxidant properties. In the present study we determined whether delphinidin would inhibit the IL-1 beta-induced activation of NF-kappa B in human chondrocytes and determined the mechanism of its action. Methods. PGE(2) levels and activation of NF-kappa B p65 in human OA chondrocytes were determined by ELISA-based assays. Protein expression of cyclo-oxygenase-2 (COX-2) and phosphorylation of kinases was determined by western immunoblotting. Expression level of mRNAs was determined by TaqMan assays. Results. Delphinidin inhibited IL-1 beta-induced expression of COX-2 and production of PGE(2) in human chondrocytes. Delphinidin also inhibited IL-1 beta-mediated phosphorylation of IL-1 receptor-associated kinase-1(Ser376), phosphorylation of IKK alpha/beta, expression of IKK beta, degradation of I kappa B alpha, and activation and nuclear translocation of NF-kappa B/p65. Phosphorylation of TGF-beta-activated kinase 1 was not observed but NF-kappa B-inducing kinase (NIK) was phosphorylated and phosphorylation of NIK was blocked by delphinidin in IL-1 beta-treated human chondrocytes. Conclusion. These data identify delphinidin as a novel inhibitor of IL-1 beta-induced production of cartilage-degrading molecule PGE(2) via inhibition of COX-2 expression and provide new insight into the mechanism of its action. Our results also identify inhibition of IRAK1(Ser376) phosphorylation by delphinidin in IL-1 beta-induced activation of NF-kappa B in human chondrocytes. Given the important role played by IL-1 beta-induced NF-kappa B activation, COX-2 expression and PGE(2) production in OA, our results may have important implications for the development of novel therapeutic strategies for the prevention/treatment of OA.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1093/rheumatology/kes363" target="_blank" rel="noreferrer noopener">10.1093/rheumatology/kes363</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2013
Chen D X
Chondrocytes
COX-2
delphinidin
Down-Regulation
factor-alpha
gene-expression
Haqqi T M
Haseeb A
human osteoarthritis chondrocytes
IL-1 beta
in-vitro
interleukin-1
Journal Article or Conference Abstract Publication
Kinase
nf-kappa-b
Osteoarthritis
Oxidative Stress
PGE(2)
rheumatoid-arthritis
Rheumatology
tumor-necrosis-factor
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1006/bbrc.1996.1412" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/bbrc.1996.1412</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
663-671
Issue
3
Volume
226
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of the hamster cholesterol 7 alpha-hydroxylase gene (CYP7A): Prevalence of negative over positive transcriptional control
Publisher
An entity responsible for making the resource available
Biochemical and Biophysical Research Communications
Date
A point or period of time associated with an event in the lifecycle of the resource
1996
1996-09
Subject
The topic of the resource
Biophysics; down-regulation; Biochemistry & Molecular Biology; hmg-coa reductase; messenger-rna levels; Bile acids; protein-kinase-c; cultures; primary; thyroid-hormone; rat hepatocytes; hormonal-regulation; hypophysectomized rats
Creator
An entity primarily responsible for making the resource
DeFabiani E; Crestani M; Marrapodi M; Pinelli A; Chiang J Y L; Galli G
Description
An account of the resource
Cholesterol 7 alpha-hydroxylase plays a crucial role in cholesterol homeostasis. We investigated the regulation of this enzyme in the hamster, a suitable animal model for studying cholesterol metabolism. DNase I hypersensitivity assay revealed the presence of a hypersensitive region in the proximal promoter. Both negative (bile acids, phorbol esters and insulin) and positive (glucocorticoid hormones) effects were mediated through sequences in the region 318 bp upstream of the ATG codon. All-trans-retinoic acid, cAMP, and LDL did not affect transcriptional activity. These findings show that the hamster cholesterol 7 alpha-hydroxylase gene undergoes a predominant negative regulation, as opposed to the rat CYP7A homologous gene. (C) 1996 Academic Press, Inc.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1006/bbrc.1996.1412" target="_blank" rel="noreferrer noopener">10.1006/bbrc.1996.1412</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1996
BILE acids
Biochemical and biophysical research communications
Biochemistry & Molecular Biology
Biophysics
Chiang J Y L
Crestani M
cultures
DeFabiani E
Down-Regulation
Galli G
HMG-CoA reductase
hormonal-regulation
hypophysectomized rats
Journal Article or Conference Abstract Publication
Marrapodi M
messenger-rna levels
Pinelli A
primary
protein-kinase-c
rat hepatocytes
thyroid-hormone
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0378-1119(03)00631-0" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0378-1119(03)00631-0</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
71-82
Volume
313
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Regulation of human sterol 27-hydroxylase gene (CYP27A1) by bile acids and hepatocyte nuclear factor 4 alpha (HNF4 alpha)
Publisher
An entity responsible for making the resource available
Gene
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-08
Subject
The topic of the resource
rat; liver; bile acid synthesis; down-regulation; hepatocytes; nuclear receptor; transcriptional regulation; negative feedback-regulation; Genetics & Heredity; cholesterol 7-alpha-hydroxylase; cholic-acid; farnesoid X receptor; heterodimer partner; x-receptor; small; alpha-fetoprotein transcription factor; cerebrotendinous xanthomatosis; factor 4-alpha
Creator
An entity primarily responsible for making the resource
Chen W L; Chiang J Y L
Description
An account of the resource
Mitochondrial sterol 27-hydroxylase (CYP27Al) catalyses sterol side-chain oxidation of bile acid synthesis front cholesterol, and the first reaction of the acidic bile acid biosynthetic pathway. Hydrophobic bile acids suppress human CYP27Al gene reporter activity when assayed in human hepatocellular blastoma HepG2 cells. Bile acids also inhibit CYP27Al reporter activity in human embryonic kidney 293 cells. A putative bile acid response element (BARE) was mapped to a region downstream of nt - 147 of the human CYP27Al gene, within which a binding site for a liver-specific nuclear receptor, HNF4alpha, is identified. HNF4alpha strongly stimulates CYP27Al gene transcription and mutation of its binding site markedly reduced promoter activity. Results suggest that human CYP27Al gene transcription is suppressed by bile acids and HNF4alpha plays a pivotal role in transcriptional regulation of this gene. (C) 2003 Elsevier Science B.V. All rights reserved.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0378-1119(03)00631-0" target="_blank" rel="noreferrer noopener">10.1016/s0378-1119(03)00631-0</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2003
alpha-fetoprotein transcription factor
Bile acid synthesis
cerebrotendinous xanthomatosis
Chen W L
Chiang J Y L
cholesterol 7-alpha-hydroxylase
cholic-acid
Down-Regulation
factor 4-alpha
Farnesoid X receptor
gene
Genetics & Heredity
hepatocytes
heterodimer partner
Journal Article or Conference Abstract Publication
Liver
negative feedback-regulation
Nuclear Receptor
rat
Small
transcriptional regulation
x-receptor
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/ajpgi.00209.2007" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/ajpgi.00209.2007</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
G817-G823
Issue
4
Volume
293
Search for Full-text
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Title
A name given to the resource
An overlapping binding site in the CYP7A1 promoter allows activation of FXR to override the stimulation by LXR alpha
Publisher
An entity responsible for making the resource available
American Journal of Physiology-Gastrointestinal and Liver Physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007-10
Subject
The topic of the resource
7-alpha-hydroxylase gene; activity; bile-acid biosynthesis; cholesterol; cholesterol 7 alpha-hydroxylase; dietary-cholesterol; down-regulation; farnesoid X; fetoprotein transcription factor; Gastroenterology & Hepatology; heterodimer partner; liver X receptor; LXR binding site; messenger-rna; metabolism; orphan nuclear receptor; Physiology; rat; receptor; regulation; short; transcriptional; x-receptor
Creator
An entity primarily responsible for making the resource
Shang Q; Pan L X; Saumoy M; Chiang J Y L; Tint G S; Salen G; Xu G R
Description
An account of the resource
An overlapping binding site in the CYP7A1 promoter allows activation of FXR to override the stimulation by LXR alpha. Am J Physiol Gastrointest Liver Physiol 293: G817-G823, 2007. First published August 9, 2007; doi:10.1152/ajpgi.00209.2007.-The aim of this study was to explore why in rabbits activation of farnesoid X receptor (FXR) is dominant over activated liver X receptor-alpha (LXR alpha) in the regulation of CYP7A1. We cloned the rabbit CYP7A1 promoter and found a fetoprotein transcription factor (FTF) binding element embedded within the LXR alpha binding site (LXRE). Gel shift assays demonstrated that FTF competes with LXR alpha for binding to LXRE. Short heterodimer partner (SHP) enhances the competitive ability of FTF. Studies in HepG2 cells showed that SHP combined with FTF had more powerful effect to offset the stimulation of CYP7A1 by LXR alpha. Gel shift and chromatin immunoprecipitation assays demonstrated that SHP with FTF diminished LXR alpha\binding to the CYP7A1 promoter. In vivo studies in rabbits fed cholesterol for 10 days showed that hepatic expression of SHP but not FTF rose and LXR alpha-bound LXRE decreased. We propose that the SHP/FTF heterodimer occupies LXRE via the embedded FTF binding element and blocks LXR alpha from recruiting to LXRE. Therefore, activation of FXR, which upregulates SHP expression, will eliminate the stimulatory effect of LXR alpha on the CYP7A1 promoter because increased levels of SHP combined with FTF diminish the recruitment of LXR alpha to CYP7A1 promoter.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/ajpgi.00209.2007" target="_blank" rel="noreferrer noopener">10.1152/ajpgi.00209.2007</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2007
7-alpha-hydroxylase gene
activity
American Journal of Physiology-Gastrointestinal and Liver Physiology
bile-acid biosynthesis
Chiang J Y L
Cholesterol
cholesterol 7 alpha-hydroxylase
dietary-cholesterol
Down-Regulation
farnesoid X
fetoprotein transcription factor
Gastroenterology & Hepatology
heterodimer partner
Journal Article
liver X receptor
LXR binding site
messenger-rna
Metabolism
orphan nuclear receptor
Pan L X
Physiology
rat
Receptor
regulation
Salen G
Saumoy M
Shang Q
short
Tint G S
transcriptional
x-receptor
Xu G R
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1152/japplphysiol.00275.2002" target="_blank" rel="noreferrer noopener">http://doi.org/10.1152/japplphysiol.00275.2002</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1875-1880
Issue
5
Volume
93
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The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Lung edema clearance: 20 years of progress selected contribution: Long-term effects of beta(2)-adrenergic receptor stimulation on alveolar fluid clearance in mice
Publisher
An entity responsible for making the resource available
Journal of Applied Physiology
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-11
Subject
The topic of the resource
acute lung injury; alveolar; beta-adrenergic-receptor; desensitization; down-regulation; epithelium; hydrostatic pulmonary-edema; in-vivo; liquid clearance; lung fluid balance; messenger-rna; Physiology; pulmonary edema; rat lung; resolution; sodium transport; Sport Sciences
Creator
An entity primarily responsible for making the resource
Sartori C; Fang X; McGraw D W; Koch P; Snider M E; Folkesson H G; Matthay M A
Description
An account of the resource
Stimulation of active fluid transport with beta-adrenergic receptor (betaAR) agonists can accelerate the resolution of alveolar edema. However, chronic betaAR-agonist administration may cause betaAR desensitization and downregulation that may impair physiological responsiveness to betaAR-agonist stimulation. Therefore, we measured baseline and terbutaline- (10(-3) M) stimulated alveolar fluid clearance in mice that received subcutaneously (miniosmotic pumps) either saline or albuterol (2 mg.kg(-1).day(-1)) for 1, 3, or 6 days. Continuous albuterol administration increased plasma albuterol levels (10(-5) M), an effect that was associated with 1) a significant decrease in betaAR density and 2) attenuation, but not ablation, of maximal terbutaline- induced cAMP production. Forskolin-mediated cAMP-release was unaffected. Continuous albuterol infusion stimulated alveolar fluid clearance on day 1 but did not increase alveolar fluid clearance on days 3 and 6. However, terbutaline- stimulated alveolar fluid clearance in albuterol-treated mice was not reduced compared with saline-treated mice. Despite significant reductions in betaAR density and agonist-mediated cAMP production by long-term betaAR-agonist exposure, maximal betaAR-agonist-mediated increase in alveolar fluid clearance is not diminished in mice.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1152/japplphysiol.00275.2002" target="_blank" rel="noreferrer noopener">10.1152/japplphysiol.00275.2002</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2002
acute lung injury
alveolar
beta-adrenergic-receptor
Desensitization
Down-Regulation
Epithelium
Fang X
Folkesson H G
hydrostatic pulmonary-edema
in-vivo
Journal Article
Journal of Applied Physiology
Koch P
liquid clearance
lung fluid balance
Matthay M A
McGraw D W
messenger-rna
Physiology
pulmonary edema
rat lung
resolution
Sartori C
Snider M E
sodium transport
Sport Sciences
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1124/dmd.32.4.367" target="_blank" rel="noreferrer noopener">http://doi.org/10.1124/dmd.32.4.367</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
367-375
Issue
4
Volume
32
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Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Transcriptional suppression of cytochrome P450 genes by endogenous and exogenous chemicals
Publisher
An entity responsible for making the resource available
Drug Metabolism and Disposition
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-04
Subject
The topic of the resource
bile acid; cholesterol 7 alpha-hydroxylase; cyp2c11 gene; cyp7a1 transcription; down-regulation; messenger-rna; nuclear receptor; Pharmacology & Pharmacy; polycyclic aromatic-hydrocarbons; pregnane X receptor; rat-liver
Creator
An entity primarily responsible for making the resource
Riddick D S; Lee C; Bhathena A; Timsit Y E; Cheng P Y; Morgan E T; Prough R A; Ripp S L; Miller K K M; Jahan A; Chiang J Y L
Description
An account of the resource
This article is an invited report of a symposium sponsored by the Division for Drug Metabolism of the American Society for Pharmacology and Experimental Therapeutics held at Experimental Biology 2003 in San Diego, California, April 11 - 15, 2003. Several members of the cytochrome P450 (P450) superfamily are induced after exposure to a variety of chemical signals, and we have gained considerable mechanistic insight into these processes over the past four decades. In addition, the expression of many P450s is suppressed in response to various endogenous and exogenous chemicals; however, relatively little is known about the molecular mechanisms involved. The goal of this symposium was to critically examine our current understanding of molecular mechanisms involved in transcriptional suppression of CYP genes by endogenous and exogenous chemicals. Specific examples were drawn from the following chemical categories: polycyclic and halogenated aromatic hydrocarbon environmental toxicants, inflammatory mediators, the endogenous sterol dehydroepiandrosterone and peroxisome proliferators, and bile acids. Multiple molecular mechanisms are involved in transcriptional suppression, and these processes often involve rather complex cascades of transcription factors and other regulatory proteins. Mechanistic studies of CYP gene suppression can enhance our understanding of how organisms respond to xenobiotics as well as to perturbations in endogenous chemicals involved in maintaining homeostasis.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1124/dmd.32.4.367" target="_blank" rel="noreferrer noopener">10.1124/dmd.32.4.367</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2004
Bhathena A
bile acid
Cheng P Y
Chiang J Y L
cholesterol 7 alpha-hydroxylase
cyp2c11 gene
cyp7a1 transcription
Down-Regulation
Drug Metabolism and Disposition
Jahan A
Journal Article
Lee C
messenger-rna
Miller K K M
Morgan E T
Nuclear Receptor
Pharmacology & Pharmacy
polycyclic aromatic-hydrocarbons
Pregnane X Receptor
Prough R A
rat-liver
Riddick D S
Ripp S L
Timsit Y E
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1369/jhc.4A6455.2004" target="_blank" rel="noreferrer noopener">http://doi.org/10.1369/jhc.4A6455.2004</a>
Pages
1665–1674
Issue
12
Volume
52
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Characterization of vascular endothelial growth factor (VEGF) in the uterine cervix over pregnancy: effects of denervation and implications for cervical ripening.
Publisher
An entity responsible for making the resource available
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-12
Subject
The topic of the resource
Female; Animals; Immunohistochemistry; Pregnancy; Rats; Microcirculation; Phosphorylation; Down-Regulation; Enzyme-Linked Immunosorbent Assay; Reverse Transcriptase Polymerase Chain Reaction; Cervical Ripening/*metabolism; Cervix Uteri/blood supply/innervation/*metabolism; Denervation; Nitric Oxide Synthase Type III; Nitric Oxide Synthase/biosynthesis; Protein Isoforms/biosynthesis/metabolism; Protein-Serine-Threonine Kinases/biosynthesis; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins/biosynthesis; Vascular Endothelial Growth Factor A/biosynthesis/*metabolism; Vascular Endothelial Growth Factor Receptor-2/biosynthesis; Wistar; Animal/*metabolism
Creator
An entity primarily responsible for making the resource
Mowa C N; Jesmin S; Sakuma I; Usip S; Togashi H; Yoshioka M; Hattori Y; Papka R
Description
An account of the resource
Bilateral neurectomy of the pelvic nerve (BLPN) that carries uterine cervix-related sensory nerves induces dystocia, and administration of its vasoactive neuropeptides induces changes in the cervical microvasculature, resembling those that occur in the ripening cervix. This study was designed to test the hypothesis that (a) the cervix of pregnant rats expresses vascular endothelial growth factor (VEGF) and components of the angiogenic signaling pathway [VEGF receptors (Flt-1, KDR), activity of protein kinase B, Akt (phosphorylated Akt), and endothelial nitric oxide synthase (eNOS)] and von Willebrand Factor (vWF) and that these molecules undergo changes with pregnancy, and (b) bilateral pelvic neurectomy (BLPN) alters levels of VEGF concentration in the cervix. Using RT-PCR and sequencing, two VEGF isoforms, 120 and 164, were identified in the rat cervix. VEGF, VEGF receptor-1 (Flt-1), eNOS, and vWF immunoreactivities (ir) were localized in the microvasculature of cervical stroma. Their protein levels increased during pregnancy but decreased to control levels by 2 days postpartum. VEGF receptor-2 (KDR)-ir was confined to the epithelium of the endocervix. BLPN downregulated levels of VEGF by a third. Therefore, the components of the angiogenic signaling pathway are expressed in the cervix and change over pregnancy. Furthermore, angiogenic and sensory neuronal factors may be important in regulating the dynamic microvasculature in the ripening cervix and may subsequently play a role in cervical ripening and the birth process.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1369/jhc.4A6455.2004" target="_blank" rel="noreferrer noopener">10.1369/jhc.4A6455.2004</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2004
Animal/*metabolism
Animals
Cervical Ripening/*metabolism
Cervix Uteri/blood supply/innervation/*metabolism
Denervation
Department of Anatomy & Neurobiology
Down-Regulation
Enzyme-Linked Immunosorbent Assay
Female
Hattori Y
Immunohistochemistry
Jesmin S
Microcirculation
Mowa C N
NEOMED College of Medicine
Nitric Oxide Synthase Type III
Nitric Oxide Synthase/biosynthesis
Papka R
Phosphorylation
Pregnancy
Protein Isoforms/biosynthesis/metabolism
Protein-Serine-Threonine Kinases/biosynthesis
Proto-Oncogene Proteins c-akt
Proto-Oncogene Proteins/biosynthesis
Rats
Reverse Transcriptase Polymerase Chain Reaction
Sakuma I
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Togashi H
Usip S
Vascular Endothelial Growth Factor A/biosynthesis/*metabolism
Vascular Endothelial Growth Factor Receptor-2/biosynthesis
Wistar
Yoshioka M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1369/jhc.2009.953893" target="_blank" rel="noreferrer noopener">http://doi.org/10.1369/jhc.2009.953893</a>
Pages
923–931
Issue
10
Volume
57
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Advanced osteoarthritis in humans is associated with altered collagen VI expression and upregulation of ER-stress markers Grp78 and bag-1.
Publisher
An entity responsible for making the resource available
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-10
Subject
The topic of the resource
Adult; Humans; Middle Aged; Osteoarthritis; Biomarkers/metabolism; Proteoglycans/biosynthesis; Down-Regulation; Up-Regulation; Cartilage; Antigens/biosynthesis; Bone Neoplasms/metabolism; Chondrosarcoma/metabolism; Collagen Type VI/*biosynthesis; DNA-Binding Proteins/*biosynthesis; Endoplasmic Reticulum/*metabolism; Heat-Shock Proteins/*biosynthesis; Osteosarcoma/metabolism; Transcription Factors/*biosynthesis; Articular/metabolism; Knee/*metabolism/physiopathology
Creator
An entity primarily responsible for making the resource
Nugent Ashleigh E; Speicher Danielle M; Gradisar Ian; McBurney Denise L; Baraga Anthony; Doane Kathleen J; Horton Walter E Jr
Description
An account of the resource
To test the hypothesis that a perturbation of endoplasmic reticulum (ER) function is involved in the pathogenesis of osteoarthritis (OA), articular cartilage was isolated from non-OA patients secondary to resection of osteo- or chondrosarcomas. Intra-joint samples of minimal and advanced osteoarthritic cartilage were isolated from patients undergoing total knee arthroplasty and scored for disease severity. Glucose-regulated protein-78 (grp78) and bcl-2-associated athanogene-1 (bag-1) were detected via immunofluorescence as markers of non-homeostatic ER function. Additionally, the expression of type VI collagen and its integrin receptor, NG2, was determined to examine cartilage matrix health and turnover. There was an upregulation of grp78 in advanced OA, and variable expression in minimal OA. Non-OA cartilage was consistently grp78 negative. The downstream regulator bag-1 was also upregulated in OA compared with normal cartilage. Collagen VI was mainly cell-associated in non-OA cartilage, with a more widespread distribution observed in OA cartilage along with increased intracellular staining intensity. The collagen VI integral membrane proteoglycan receptor NG2 was downregulated in advanced OA compared with its patient-matched minimally involved cartilage sample. These results suggest that chondrocytes exhibit ER stress during OA, in association with upregulation of a large secreted molecule, type VI collagen.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1369/jhc.2009.953893" target="_blank" rel="noreferrer noopener">10.1369/jhc.2009.953893</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
Adult
Antigens/biosynthesis
Articular/metabolism
Baraga Anthony
Biomarkers/metabolism
Bone Neoplasms/metabolism
Cartilage
Chondrosarcoma/metabolism
Collagen Type VI/*biosynthesis
Department of Anatomy & Neurobiology
DNA-Binding Proteins/*biosynthesis
Doane Kathleen J
Down-Regulation
Endoplasmic Reticulum/*metabolism
Gradisar Ian
Heat-Shock Proteins/*biosynthesis
Horton Walter E Jr
Humans
Knee/*metabolism/physiopathology
McBurney Denise L
Middle Aged
NEOMED College of Medicine
Nugent Ashleigh E
Osteoarthritis
Osteosarcoma/metabolism
Proteoglycans/biosynthesis
Speicher Danielle M
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Transcription Factors/*biosynthesis
Up-Regulation
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1136/gutjnl-2016-311861" target="_blank" rel="noreferrer noopener">http://doi.org/10.1136/gutjnl-2016-311861</a>
Pages
705–715
Issue
4
Volume
66
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
MicroRNA-223 ameliorates alcoholic liver injury by inhibiting the
Publisher
An entity responsible for making the resource available
Gut
Date
A point or period of time associated with an event in the lifecycle of the resource
2017
2017-04
Subject
The topic of the resource
*CYTOKINES; *ETHANOL; *FATTY LIVER; *INFLAMMATION; *LEUKOCYTES; Adult; Alanine Transaminase/blood; Alcoholic/genetics/*metabolism/pathology; Alcoholism/*blood/complications; Animals; Aspartate Aminotransferases/blood; Bilirubin/blood; Binge Drinking/*blood/complications; Case-Control Studies; Central Nervous System Depressants/administration & dosage; Down-Regulation; Ethanol/administration & dosage; Female; Humans; Inbred C57BL; Interleukin-6/genetics/metabolism; Liver Diseases; Male; Mice; MicroRNAs/*blood/*genetics; Middle Aged; NADPH Oxidases/genetics/metabolism; Neutrophils/*metabolism; Reactive Oxygen Species/metabolism; Up-Regulation; Young Adult
Creator
An entity primarily responsible for making the resource
Li Man; He Yong; Zhou Zhou; Ramirez Teresa; Gao Yueqiu; Gao Yanhang; Ross Ruth A; Cao Haixia; Cai Yan; Xu Ming-Jiang; Feng Dechun; Zhang Ping; Liangpunsakul Suthat; Gao Bin
Description
An account of the resource
OBJECTIVES: Chronic-plus-binge ethanol feeding activates neutrophils and exacerbates liver injury in mice. This study investigates how recent excessive drinking affects peripheral neutrophils and liver injury in alcoholics, and how miR-223, one of the most abundant microRNAs (miRNAs) in neutrophils, modulates neutrophil function and liver injury in ethanol-fed mice. DESIGNS: Three hundred alcoholics with (n=140) or without (n=160) recent excessive drinking and 45 healthy controls were enrolled. Mice were fed an ethanol diet for 10 days followed by a single binge of ethanol. RESULTS: Compared with healthy controls or alcoholics without recent drinking, alcoholics with recent excessive drinking had higher levels of circulating neutrophils, which correlated with serum levels of alanine transaminase (ALT) and aspartate transaminase (AST). miRNA array analysis revealed that alcoholics had elevated serum miR-223 levels compared with healthy controls. In chronic-plus-binge ethanol feeding mouse model, the levels of miR-223 were increased in both serum and neutrophils. Genetic deletion of the miR-223 gene exacerbated ethanol-induced hepatic injury, neutrophil infiltration, reactive oxygen species (ROS) and upregulated hepatic expression of interleukin (IL)-6 and phagocytic oxidase (phox) p47(phox). Mechanistic studies revealed that miR-223 directly inhibited IL-6 expression and subsequently inhibited p47(phox) expression in neutrophils. Deletion of the p47(phox) gene ameliorated ethanol-induced liver injury and ROS production by neutrophils. Finally, miR-223 expression was downregulated, while IL-6 and p47(phox) expression were upregulated in peripheral blood neutrophils from alcoholics compared with healthy controls. CONCLUSIONS: miR-223 is an important regulator to block neutrophil infiltration in alcoholic liver disease and could be a novel therapeutic target for the treatment of this malady.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1136/gutjnl-2016-311861" target="_blank" rel="noreferrer noopener">10.1136/gutjnl-2016-311861</a>
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Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*CYTOKINES
*ETHANOL
*FATTY LIVER
*Inflammation
*LEUKOCYTES
2017
Adult
Alanine Transaminase/blood
Alcoholic/genetics/*metabolism/pathology
Alcoholism/*blood/complications
Animals
Aspartate Aminotransferases/blood
Bilirubin/blood
Binge Drinking/*blood/complications
Cai Yan
Cao Haixia
Case-Control Studies
Central Nervous System Depressants/administration & dosage
Department of Integrative Medical Sciences
Department of Pharmaceutical Sciences
Down-Regulation
Ethanol/administration & dosage
Female
Feng Dechun
Gao Bin
Gao Yanhang
Gao Yueqiu
Gut
He Yong
Humans
Inbred C57BL
Interleukin-6/genetics/metabolism
Li Man
Liangpunsakul Suthat
Liver Diseases
Male
Mice
MicroRNAs/*blood/*genetics
Middle Aged
NADPH Oxidases/genetics/metabolism
NEOMED College of Medicine
NEOMED College of Pharmacy
Neutrophils/*metabolism
Ramirez Teresa
Reactive Oxygen Species/metabolism
Ross Ruth A
Up-Regulation
Xu Ming-Jiang
Young Adult
Zhang Ping
Zhou Zhou
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M502751200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M502751200</a>
Pages
30517–30525
Issue
34
Volume
280
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Bcl-2 positively regulates Sox9-dependent chondrocyte gene expression by suppressing the MEK-ERK1/2 signaling pathway.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-08
Subject
The topic of the resource
*Gene Expression Regulation; Adenoviridae/genetics; Animals; Apoptosis; beta-Galactosidase/metabolism; Blotting; Butadienes/pharmacology; Caspase Inhibitors; Cell Differentiation; Cell Line; Chondrocytes/*metabolism; Collagen Type II/metabolism; Down-Regulation; Enzyme Inhibitors/pharmacology; Fibroblasts/metabolism; Fluorescence; Genetic; High Mobility Group Proteins/*metabolism; Lac Operon; Luciferases/metabolism; MAP Kinase Kinase Kinases/*metabolism; Messenger/metabolism; Microscopy; Mitogen-Activated Protein Kinase 1/*metabolism; Mitogen-Activated Protein Kinase 3/*metabolism; NF-kappa B/metabolism; Nitriles/pharmacology; Phenotype; Phosphorylation; Promoter Regions; Protein Kinase C-alpha; Protein Kinase C/antagonists & inhibitors; Proteoglycans/metabolism; Proto-Oncogene Proteins c-bcl-2/*metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction; RNA; Signal Transduction; Small Interfering/metabolism; SOX9 Transcription Factor; Sprague-Dawley; Time Factors; Transcription; Transcription Factors/*metabolism; Transfection; Western
Creator
An entity primarily responsible for making the resource
Yagi Rieko; McBurney Denise; Horton Walter E Jr
Description
An account of the resource
Bcl-2 is an anti-apoptotic protein that has recently been shown to regulate other cellular functions. We previously reported that Bcl-2 regulates chondrocyte matrix gene expression, independent of its anti-apoptotic function. Here, we further investigate this novel function of Bcl-2 and examine three intracellular signaling pathways likely to be associated with this function. The present study demonstrates that the activity of Sox9, a master transcription factor that regulates the gene expression of chondrocyte matrix proteins, is suppressed by Bcl-2 small interference RNA in the presence of caspase inhibitors. This effect was attenuated by prior exposure of chondrocytes to an adenoviral vector expressing sense Bcl-2. In addition, the down-regulation of Bcl-2, Sox9, and chondrocyte-specific gene expression by serum withdrawal in primary chondrocytes was reversed by expressing Bcl-2. Inhibition of the protein kinase C alpha and NFkappaB pathways had no effect on the maintenance of Sox9-dependent gene expression by Bcl-2. In contrast, whereas the MEK-ERK1/2 pathway negatively regulated the differentiated phenotype in wild type chondrocytes, inhibition of this pathway reversed the loss of differentiation markers and fibroblastic phenotype in Bcl-2-deficient chondrocytes. In conclusion, the present study identifies a specific signaling pathway, namely, MEK-ERK1/2, that is downstream of Bcl-2 in the regulation of Sox9-dependent chondrocyte gene expression and phenotype.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M502751200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M502751200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Gene Expression Regulation
2005
Adenoviridae/genetics
Animals
Apoptosis
beta-Galactosidase/metabolism
Blotting
Butadienes/pharmacology
Caspase Inhibitors
Cell Differentiation
Cell Line
Chondrocytes/*metabolism
Collagen Type II/metabolism
Department of Anatomy & Neurobiology
Down-Regulation
Enzyme Inhibitors/pharmacology
Fibroblasts/metabolism
Fluorescence
Genetic
High Mobility Group Proteins/*metabolism
Horton Walter E Jr
Lac Operon
Luciferases/metabolism
MAP Kinase Kinase Kinases/*metabolism
McBurney Denise
Messenger/metabolism
Microscopy
Mitogen-Activated Protein Kinase 1/*metabolism
Mitogen-Activated Protein Kinase 3/*metabolism
NEOMED College of Medicine
NF-kappa B/metabolism
Nitriles/pharmacology
Phenotype
Phosphorylation
Promoter Regions
Protein Kinase C-alpha
Protein Kinase C/antagonists & inhibitors
Proteoglycans/metabolism
Proto-Oncogene Proteins c-bcl-2/*metabolism
Rats
Reverse Transcriptase Polymerase Chain Reaction
RNA
Signal Transduction
Small Interfering/metabolism
SOX9 Transcription Factor
Sprague-Dawley
The Journal of biological chemistry
Time Factors
Transcription
Transcription Factors/*metabolism
Transfection
Western
Yagi Rieko
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1038/emm.2015.66" target="_blank" rel="noreferrer noopener">http://doi.org/10.1038/emm.2015.66</a>
Pages
e189–e189
Volume
47
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
miR-139 modulates MCPIP1/IL-6 expression and induces apoptosis in human OA chondrocytes.
Publisher
An entity responsible for making the resource available
Experimental & molecular medicine
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015-10
Subject
The topic of the resource
*Apoptosis; 3' Untranslated Regions; Aged; Chondrocytes/*metabolism/pathology; Down-Regulation; Female; Gene Expression Regulation; Humans; Interleukin-6/*genetics; Male; Messenger/genetics; MicroRNAs/*genetics; Middle Aged; Osteoarthritis/*genetics/pathology; Ribonucleases/*genetics; RNA; Transcription Factors/*genetics; Up-Regulation
Creator
An entity primarily responsible for making the resource
Makki Mohammad Shahidul; Haqqi Tariq M
Description
An account of the resource
IL-6 is an inflammatory cytokine and its overexpression plays an important role in osteoarthritis (OA) pathogenesis. Expression of IL-6 is regulated post-transcriptionally by MCPIP1. The 3' untranslated region (UTR) of MCPIP1 mRNA harbors a miR-139 'seed sequence', therefore we examined the post-transcriptional regulation of MCPIP1 by miR-139 and its impact on IL-6 expression in OA chondrocytes. Expression of miR-139 was found to be high in the damaged portion of the OA cartilage compared with unaffected cartilage from the same patient and was also induced by IL-1beta in OA chondrocytes. Inhibition of miR-139 decreased the expression of IL-6 mRNA by 38% and of secreted IL-6 protein by 40%. However, overexpression of miR-139 increased the expression of IL-6 mRNA by 36% and of secreted IL-6 protein by 56%. These data correlated with altered expression profile of MCPIP1 in transfected chondrocytes. Studies with a luciferase reporter construct confirmed the interactions of miR-139 with the 'seed sequence' located in the 3' UTR of MCPIP mRNA. Furthermore, miR-139 overexpression increased the catabolic gene expression but expression of anabolic markers remained unchanged. Overexpression of miR-139 also induced apoptosis in OA chondrocytes. Importantly, we also discovered that IL-6 is a potent inducer of miR-139 expression in OA chondrocytes. These findings indicate that miR-139 functions as a post-transcriptional regulator of MCPIP1 expression and enhances IL-6 expression, which further upregulates miR-139 expression in OA chondrocytes. These results support our hypothesis that miR-139-mediated downregulation of MCPIP1 promotes
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1038/emm.2015.66" target="_blank" rel="noreferrer noopener">10.1038/emm.2015.66</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Apoptosis
2015
3' Untranslated Regions
Aged
Chondrocytes/*metabolism/pathology
Department of Anatomy & Neurobiology
Down-Regulation
Experimental & molecular medicine
Female
Gene Expression Regulation
Haqqi Tariq M
Humans
Interleukin-6/*genetics
Makki Mohammad Shahidul
Male
Messenger/genetics
MicroRNAs/*genetics
Middle Aged
NEOMED College of Medicine
Osteoarthritis/*genetics/pathology
Ribonucleases/*genetics
RNA
Transcription Factors/*genetics
Up-Regulation
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s0196-9781(03)00120-7" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s0196-9781(03)00120-7</a>
Pages
761–771
Issue
5
Volume
24
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Substance P in the uterine cervix, dorsal root ganglia and spinal cord during pregnancy and the effect of estrogen on SP synthesis.
Publisher
An entity responsible for making the resource available
Peptides
Date
A point or period of time associated with an event in the lifecycle of the resource
2003
2003-05
Subject
The topic of the resource
Afferent/metabolism; Animals; Cervix Uteri/cytology/*metabolism; Down-Regulation; Estradiol/*analogs & derivatives/pharmacology; Estrogen/antagonists & inhibitors; Estrogens/*pharmacology; Female; Fulvestrant; Ganglia; Immunohistochemistry; In Situ Hybridization; Messenger/genetics/metabolism; Neurons; Postpartum Period; Pregnancy/*metabolism; Radioimmunoassay; Rats; Receptors; Reverse Transcriptase Polymerase Chain Reaction; RNA; Spinal Cord/cytology/*metabolism; Spinal/cytology/*metabolism; Sprague-Dawley; Substance P/*biosynthesis/genetics/metabolism; Up-Regulation
Creator
An entity primarily responsible for making the resource
Mowa C N; Usip S; Storey-Workley M; Amann R; Papka R
Description
An account of the resource
Prior to parturition the non-pliable uterine cervix undergoes a ripening process ("softens" and dilates) to allow a timely passage of the fetus at term. The exact mechanism(s) triggering and involved in cervical ripening are unknown, though evidence for a role for sensory neurons and their contained neuropeptides is emerging. Moreover, an apparent increase in neuropeptide immunoreactive nerves occurs in the cervix during pregnancy, maternal serum estrogen levels rise at term and uterine cervix-related L6-S1 dorsal root ganglia (DRG) sensory neurons express estrogen receptor (ER) and neuropeptides. Thus, we sought to test the hypothesis that the neuropeptide substance P (SP) changes biosynthesis and release over pregnancy, that estrogen, acting via the ER pathway, increases synthesis of SP in DRG, and that SP is utilized in cervical ripening at late pregnancy. Using immunohistochemistry, in situ hybridization, reverse transcriptase-polymerase chain reaction (RT-PCR) and radioimmunoassay (RIA), we investigated coexpression of ER-alpha/beta and SP; differential expression of
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/s0196-9781(03)00120-7" target="_blank" rel="noreferrer noopener">10.1016/s0196-9781(03)00120-7</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2003
Afferent/metabolism
Amann R
Animals
Cervix Uteri/cytology/*metabolism
Department of Anatomy & Neurobiology
Down-Regulation
Estradiol/*analogs & derivatives/pharmacology
Estrogen/antagonists & inhibitors
Estrogens/*pharmacology
Female
Fulvestrant
Ganglia
Immunohistochemistry
In Situ Hybridization
Messenger/genetics/metabolism
Mowa C N
NEOMED College of Medicine
Neurons
Papka R
Peptides
Postpartum Period
Pregnancy/*metabolism
Radioimmunoassay
Rats
Receptors
Reverse Transcriptase Polymerase Chain Reaction
RNA
Spinal Cord/cytology/*metabolism
Spinal/cytology/*metabolism
Sprague-Dawley
Storey-Workley M
Substance P/*biosynthesis/genetics/metabolism
Up-Regulation
Usip S
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.jnutbio.2010.09.001" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.jnutbio.2010.09.001</a>
Pages
1035–1046
Issue
11
Volume
22
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Anthocyanin-rich black currant (Ribes nigrum L.) extract affords chemoprevention against diethylnitrosamine-induced hepatocellular carcinogenesis in rats.
Publisher
An entity responsible for making the resource available
The Journal of nutritional biochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-11
Subject
The topic of the resource
Animals; Anthocyanins/*therapeutic use; Anticarcinogenic Agents/therapeutic use; Apoptosis/drug effects; bcl-2-Associated X Protein/metabolism; Cell Proliferation/drug effects; Chemoprevention; Diethylnitrosamine; Down-Regulation; Experimental/metabolism/pathology; Liver Neoplasms; Liver Neoplasms/chemically induced/*prevention & control; Liver/pathology; Male; Phenobarbital; Plant Extracts/*therapeutic use; Proliferating Cell Nuclear Antigen/metabolism; Proto-Oncogene Proteins c-bcl-2/metabolism; Rats; Ribes/chemistry; Sprague-Dawley; Up-Regulation
Creator
An entity primarily responsible for making the resource
Bishayee Anupam; Mbimba Thomas; Thoppil Roslin J; Haznagy-Radnai Erzsebet; Sipos Peter; Darvesh Altaf S; Folkesson Hans G; Hohmann Judit
Description
An account of the resource
Anthocyanins are known to possess potent anticarcinogenic properties against several cancers thus demonstrating potential for cancer prevention. Black currant (Ribes nigrum L., Grossulariaceae) fruits have a high anthocyanin content. This "superfruit" is known to possess various pharmacological effects including alleviation of chronic oxidative stress and inflammation. In contrast to a large volume of literature on the health benefits of black currant, limited evidence on antitumor effects of black currant exists with virtually no data on the prevention of experimental carcinogenesis. In the current study, we have investigated the chemopreventive effects of an anthocyanin-rich black currant skin extract (BCSE) utilizing our well-characterized model of rat liver carcinogenesis. Initiation of hepatocarcinogenesis was done by intraperitoneal injection of diethylnitrosamine (DENA) followed by promotion with phenobarbital. The rats were exposed to dietary BCSE for 4 weeks prior to initiation, and the treatment was continued for 22 consecutive weeks. BCSE dose-dependently decreased the incidence, total number, multiplicity, size and volume of preneoplastic hepatic nodules. The antihepatocarcinogenic effect of BCSE was confirmed by histopathological examination of liver sections. Immunohistochemical analysis of proliferating cell nuclear antigen and DNA fragmentation revealed BCSE-mediated inhibition of abnormal cell proliferation and induction of apoptosis in
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.jnutbio.2010.09.001" target="_blank" rel="noreferrer noopener">10.1016/j.jnutbio.2010.09.001</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2011
Animals
Anthocyanins/*therapeutic use
Anticarcinogenic Agents/therapeutic use
Apoptosis/drug effects
bcl-2-Associated X Protein/metabolism
Bishayee Anupam
Cell Proliferation/drug effects
Chemoprevention
Darvesh Altaf S
Department of Pharmaceutical Sciences
Diethylnitrosamine
Down-Regulation
Experimental/metabolism/pathology
Folkesson Hans G
Haznagy-Radnai Erzsebet
Hohmann Judit
Liver Neoplasms
Liver Neoplasms/chemically induced/*prevention & control
Liver/pathology
Male
Mbimba Thomas
NEOMED College of Pharmacy
Phenobarbital
Plant Extracts/*therapeutic use
Proliferating Cell Nuclear Antigen/metabolism
Proto-Oncogene Proteins c-bcl-2/metabolism
Rats
Ribes/chemistry
Sipos Peter
Sprague-Dawley
The Journal of nutritional biochemistry
Thoppil Roslin J
Up-Regulation
-
Text
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URL Address
<a href="http://doi.org/10.1002/jcb.10442" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcb.10442</a>
Pages
941–953
Issue
5
Volume
88
Dublin Core
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Title
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Coordinate down-regulation of cartilage matrix gene expression in Bcl-2 deficient chondrocytes is associated with decreased SOX9 expression and decreased mRNA stability.
Publisher
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Journal of cellular biochemistry
Date
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2003
2003-04
Subject
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Aggrecans; Animals; C-Type; Cell Line; Chondrocytes/*physiology; Collagen Type II/genetics/metabolism; Dactinomycin; Down-Regulation; Extracellular Matrix Proteins/analysis/genetics/*metabolism; Gene Expression Regulation; Glycoproteins/analysis/genetics/*metabolism; High Mobility Group Proteins/genetics/*metabolism; Lectins; Matrilin Proteins; Messenger/analysis/biosynthesis; Polymerase Chain Reaction/methods; Proteoglycans/genetics/metabolism; Proto-Oncogene Proteins c-bcl-2/deficiency/genetics/*physiology; Rats; RNA; RNA Stability; Signal Transduction; SOX9 Transcription Factor; Transcription Factors/genetics/*metabolism; Transfection
Creator
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Kinkel Mary D; Horton Walter E Jr
Description
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The anti-apoptotic protein Bcl-2 has been shown to function in roles unrelated to apoptosis in a variety of cell types. We have previously reported that loss of Bcl-2 expression alters chondrocyte morphology and modulates aggrecan expression via an apoptosis-independent pathway. Here we show that Bcl-2 is required for chondrocytes to maintain expression of a variety of cartilage-specific matrix proteins. Using quantitative, real-time PCR, we demonstrate that Bcl-2-deficient chondrocytes coordinately down-regulate genes coding for hyaline cartilage matrix proteins including collagen II, collagen IX, aggrecan, and link protein. The decrease in steady-state level of these mRNA transcripts results, in part, from decreased mRNA stability in Bcl-2-deficient chondrocytes. Transcriptional regulation is also likely involved because chondrocytes with decreased Bcl-2 levels show decreased expression of SOX9, a transcription factor necessary for expressing the major cartilage matrix proteins. In contrast, chondrocytes constitutively expressing Bcl-2 have a stable phenotype when subjected to loss of serum factor signaling. These cells maintain high levels of SOX9, as well as the SOX9 targets collagen II and aggrecan. These results suggest that Bcl-2 is involved in a pathway important for maintaining a stable chondrocyte phenotype.
Identifier
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<a href="http://doi.org/10.1002/jcb.10442" target="_blank" rel="noreferrer noopener">10.1002/jcb.10442</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2003
Aggrecans
Animals
C-Type
Cell Line
Chondrocytes/*physiology
Collagen Type II/genetics/metabolism
Dactinomycin
Down-Regulation
Extracellular Matrix Proteins/analysis/genetics/*metabolism
Gene Expression Regulation
Glycoproteins/analysis/genetics/*metabolism
High Mobility Group Proteins/genetics/*metabolism
Horton Walter E Jr
Journal of cellular biochemistry
Kinkel Mary D
Lectins
Matrilin Proteins
Messenger/analysis/biosynthesis
Polymerase Chain Reaction/methods
Proteoglycans/genetics/metabolism
Proto-Oncogene Proteins c-bcl-2/deficiency/genetics/*physiology
Rats
RNA
RNA Stability
Signal Transduction
SOX9 Transcription Factor
Transcription Factors/genetics/*metabolism
Transfection