Differential Expression and Cellular Distribution of gamma-Tubulin and beta III-Tubulin in Medulloblastomas and Human Medulloblastoma Cell Lines
ring; Physiology; Cell Biology; complex; mammalian-cells; monoclonal-antibodies; candidate genes; cerebellar medulloblastomas; kinesin-like protein; microtubule nucleation; mitotic spindle; pale islands; posttranslational modification
In previous studies, we have shown overexpression and ectopic subcellular distribution of gamma-tubulin and beta III-tubulin in human glioblastomas and glioblastoma cell lines (Katsetos et al., 2006,] Neuropathol Exp Neurol 65:455-467; Katsetos et al., 2007, Neurochem Res 32:1387-1398). Here we determined the expression of gamma-tubulin in surgically excised medulloblastomas (n = 20) and in the human medulloblastoma cell lines D283 Med and DAOY. In clinical tissue samples, the immunohistochemical distribution of gamma-tubulin labeling was pervasive and inversely related to neuritogenesis. Overexpression of gamma-tubulin was widespread in poorly differentiated, proliferating tumor cells but was significantly diminished in quiescent differentiating tumor cells undergoing neuritogenesis, highlighted by beta III-tubulin immunolabeling. By quantitative real-time PCR, gamma-tubulin transcripts for TUBG1, TUBG2, and TUBB3 genes were detected in both cell lines but expression was less prominent when compared with the human glioblastoma cell lines. Immunoblotting revealed comparable amounts of gamma-tubulin and beta III-tubulin in different phases of cell cycle; however, a larger amount of gamma-tubulin was detected in D283 Med when compared with DAOY cells. Interphase D283 Med cells exhibited predominantly diffuse cytoplasmic gamma-tubulin localization, in addition to the expected centrosome-associated distribution. Robust beta III-tubulin immunoreactivity was detected in mitotic spindles of DAOY cells. Our data indicate that overexpression of gamma-tubulin may be linked to phenotypic dedifferentiation (anaplasia) and tumor progression in medulloblastomas and may potentially serve as a promising tumor marker. J. Cell. Physiol. 223: 519-529,2010. (C) 2010 Wiley-Liss, Inc.
Caracciolo V; D'Agostino L; Draberova E; Sladkova V; Crozier-Fitzgerald C; Agamanolis D P; De Chadarevian J P; Legido A; Giordano A; Draber P; Katsetos C D
Journal of Cellular Physiology
2010
2010-05
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1002/jcp.22077" target="_blank" rel="noreferrer noopener">10.1002/jcp.22077</a>
Class Iii Beta-tubulin Is Constitutively Coexpressed With Glial Fibrillary Acidic Protein And Nestin In Midgestational Human Fetal Astrocytes: Implications For Phenotypic Identity
adult mammalian; astrocytes; brain; class III beta-tubulin; fetal glia; gamma-tubulin; glial fibrillary acidic; microtubule associated protein 2; microtubule associated protein 2; monoclonal-antibody; nestin; neural stem cells; neural stem cells; neuronal differentiation; Neurosciences & Neurology; Pathology; posttranslational modification; protein; radial glia; spinal cord; subventricular zone; ventricular/subventricular zone
Draberova E; Del Valle L; Gordon J; Markova V; Smejkalova B; Bertrand L; de Chadarevian J P; Agamanolis D P; Legido A; Khalili K; Draber P; Katsetos C D
Journal of Neuropathology and Experimental Neurology
2008
2008-04
Journal Article or Conference Abstract Publication
<a href="http://doi.org/10.1097/NEN.0b013e31816a686d" target="_blank" rel="noreferrer noopener">10.1097/NEN.0b013e31816a686d</a>