1
40
6
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
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n/a
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
A562-A563
Issue
4
Volume
16
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Title
A name given to the resource
On the mechanism of bile acid inhibition of rat sterol 12 alpha -hydroxylase gene (CYP8B1) transcription by nuclear receptors, alpha -fetoprotein transcription factor (FTF) and hepatocyte nuclear receptor 4 alpha (HNF4 alpha )
Publisher
An entity responsible for making the resource available
Faseb Journal
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-03
Subject
The topic of the resource
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Creator
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Chiang J Y L; Yang Y; Zhang M; Eggertsen G
Identifier
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n/a
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2002
Biochemistry & Molecular Biology
Cell Biology
Chiang J Y L
Eggertsen G
Faseb Journal
Journal Article or Conference Abstract Publication
Life Sciences & Biomedicine - Other
Topics
Yang Y
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1111/j.1432-1033.1993.tb18082.x" target="_blank" rel="noreferrer noopener">http://doi.org/10.1111/j.1432-1033.1993.tb18082.x</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
705-710
Issue
3
Volume
215
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Title
A name given to the resource
CHOLESTEROL 7-ALPHA-HYDROXYLASE IS UP-REGULATED BY THE COMPETITIVE INHIBITOR 7-OXOCHOLESTEROL IN RAT-LIVER
Publisher
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European Journal of Biochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
1993
1993-08
Subject
The topic of the resource
Biochemistry & Molecular Biology; expression; cloning; dietary-cholesterol; purification; messenger-rna; circadian-rhythm; reductase; microsomes; sequence; serum-cholesterol
Creator
An entity primarily responsible for making the resource
Breuer O; Sudjanasugiaman E; Eggertsen G; Chiang J Y L; Bjorkhem I
Description
An account of the resource
Rats of the Sprague-Dawley strain were infused intravenously with a fat emulsion (Intralipid, trademark of Kabi Pharmacia, Uppsala, Sweden) containing 7-oxocholesterol. This resulted in an increased cholesterol 7alpha-hydroxylase activity in liver microsomes as compared to controls and was accompanied by increased levels of cholesterol 7alpha-hydroxylase mRNA and microsomal cholesterol 7alpha-hydroxylase protein. Rats were also fed a cholestyramine-supplemented diet and infused with 7-oxocholesterol. These animals excreted about half as much bile acids in faeces as cholestyramine-fed controls. Addition of 7-oxocholesterol to liver microsomes from normal rats in amounts corresponding to those present in microsomes from 7-oxocholesterol-treated rats inhibited the cholesterol 7alpha-hydroxylase activity by about 75%. Cholesterol induced a type-I binding spectrum when added to a purified bacterial-expressed cholesterol 7alpha-hydroxylase (P-450c7DELTA2-24). 7-Oxocholesterol competitively inhibited the cholesterol binding spectrum, while 7beta-hydroxycholesterol did not interfere with binding of cholesterol to the enzyme. It is concluded that treatment with the competitive inhibitor 7-oxocholesterol leads to a reduced bile acid biosynthesis and, as a consequence of reduced bile acid inhibition, a compensatory increase in cholesterol 7alpha-hydroxylase synthesis. The high enzyme activity measured in microsomal preparations from 7-oxocholesterol-treated rats may be due to a continuous conversion of 7-oxocholesterol into less inhibitory metabolites, e.g. 7beta-hydroxycholesterol. The latter compound was found in high concentrations in liver microsomes from rats treated with 7-oxocholesterol. The physiological importance of these results is discussed in relation to the previous findings that 7-oxocholesterol is accumulated in liver after cholesterol feeding and that 7-oxocholesterol is formed from cholesterol during lipid peroxidation.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1111/j.1432-1033.1993.tb18082.x" target="_blank" rel="noreferrer noopener">10.1111/j.1432-1033.1993.tb18082.x</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
1993
Biochemistry & Molecular Biology
Bjorkhem I
Breuer O
Chiang J Y L
circadian-rhythm
Cloning
dietary-cholesterol
Eggertsen G
European Journal of Biochemistry
expression
Journal Article or Conference Abstract Publication
messenger-rna
Microsomes
purification
reductase
sequence
serum-cholesterol
Sudjanasugiaman E
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
n/a
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
27-27
Volume
94
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Title
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Developmental and estrogen-mediated regulation of neurosteroid hydroxylase CYP7B1 in humans
Publisher
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Journal of Neurochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-08
Subject
The topic of the resource
Biochemistry & Molecular Biology; Neurosciences & Neurology
Creator
An entity primarily responsible for making the resource
Tang W; Eggertsen G; Chiang J Y; Norlin M
Identifier
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n/a
Format
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Journal Article
2005
Biochemistry & Molecular Biology
Chiang J Y
Eggertsen G
Journal Article
Journal of neurochemistry
Neurosciences & Neurology
Norlin M
Tang W
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.bbrc.2004.06.069" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.bbrc.2004.06.069</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
1204-1210
Issue
4
Volume
320
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Title
A name given to the resource
Mechanisms of cholesterol and sterol regulatory element binding protein regulation of the sterol 12 alpha-hydroxylase gene (CYP8B1)
Publisher
An entity responsible for making the resource available
Biochemical and Biophysical Research Communications
Date
A point or period of time associated with an event in the lifecycle of the resource
2004
2004-08
Subject
The topic of the resource
bile acids; bile-acid metabolism; Biochemistry & Molecular Biology; Biophysics; cholesterol; CYP8B1; gene regulation; liver; mice; mouse; pathway; promoter; rat; receptor lxr-alpha; SREBP; srebps; suppresses
Creator
An entity primarily responsible for making the resource
Yang Y Z; Eggertsen G; Gafvels M; Andersson U; Einarsson C; Bjorkhem I; Chiang J Y L
Description
An account of the resource
Sterol 12alpha-hydroxylase (CYP8B1) is an obligatory enzyme for the synthesis of cholic acid and regulation of liver bile acid synthesis and intestine cholesterol absorption. The present study evaluates the roles for sterol regulatory element binding proteins (SREBPs) in the regulation of the CYP8B1 gene. Cholesterol feeding of mice and rats decreased the activity of CYP8B1, contrary to the up-regulation of cholesterol 7alpha-hydroxylase (CYP7A1). Cholesterol feeding also reduced mRNA levels for SREBP-1 but not for SREBP-2 in rat livers. Cholesterol and 25-hydroxycholesterol decreased the CYP8B1/luciferase reporter activity. Co-transfection of SREBP-1a and -1c stimulated CYP8B1 promoter activity, while SREBP-2 did not have any effects. Electrophoretic mobility shift assay and mutagenesis analyses identified several functional sterol regulatory elements (SRE) and E-box motifs in the rat CYP8B1 promoter. Our results indicate that SREBP-1a and -1c enhance transcription of the CYP8B1 gene through binding to SRE. Cholesterol loading reduces SREBP-1 mRNA expression in addition to reducing functional SREBP-1 protein, and results in decreasing CYP8B1 gene transcription. (C) 2004 Elsevier Inc. All rights reserved.
Identifier
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<a href="http://doi.org/10.1016/j.bbrc.2004.06.069" target="_blank" rel="noreferrer noopener">10.1016/j.bbrc.2004.06.069</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2004
Andersson U
BILE acids
bile-acid metabolism
Biochemical and biophysical research communications
Biochemistry & Molecular Biology
Biophysics
Bjorkhem I
Chiang J Y L
Cholesterol
CYP8B1
Eggertsen G
Einarsson C
Gafvels M
Gene Regulation
Journal Article
Liver
Mice
mouse
pathway
promoter
rat
receptor lxr-alpha
SREBP
srebps
suppresses
Yang Y Z
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/s1388-1981(02)00186-5" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/s1388-1981(02)00186-5</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
63-73
Issue
1
Volume
1583
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Title
A name given to the resource
On the mechanism of bile acid inhibition of rat sterol 12 alpha-hydroxylase gene (CYP8B1) transcription: roles of alpha-fetoprotein transcription factor and hepatocyte nuclear factor 4 alpha
Publisher
An entity responsible for making the resource available
Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids
Date
A point or period of time associated with an event in the lifecycle of the resource
2002
2002-06
Subject
The topic of the resource
activation; bile acid synthesise; Biochemistry & Molecular Biology; Biophysics; biosynthesis; Cell Biology; cholesterol 7-alpha-hydroxylase gene; Cyp7a1; cytochrome P450; expression; feedback-regulation; gene regulation; liver microsomal metabolism; nuclear receptor; promoter; receptor; repression
Creator
An entity primarily responsible for making the resource
Yang Y Z; Zhang M; Eggertsen G; Chiang J Y L
Description
An account of the resource
The sterol 12alpha-hydroxylase (CYP8B1) is a key enzyme of the bile acid biosynthetic pathway. It regulates the composition of bile acids in bile, i.e. ratio between cholic acid (CA) and chenodeoxycholic acid (CDCA). In similarity with cholesterol 7alpha-hydroxylase (CYP7A1), this enzyme is subjected to a negative feedback regulation by bile acids, It has been recently reported that bile acid-activated famesoid X receptor (FXR) induces the small heterodimer partner (SHP) that interacts with alpha-fetoprotein transcription factor (FTF) and down-regulates CYP7A1 transcription. We studied whether the same mechanism also regulated rat CYP8B1 gene transcription. Feeding rats with CDCA caused a 40-50% decrease of CYP8B1 and hepatocyte nuclear factor 4alpha (HNF4alpha) mRNA expression levels. This was associated with an increase in FTF mRNA expression, but SHP mRNA expression was not altered. Electrophoretic mobility shift assay (EMSA) and transient transfection assay of promoter/reporter genes coupled to mutagenesis analysis identified a putative bile acid response element (BARE) that has an HNF4alpha binding site embedded in two overlapping FTF binding sites. Mutation of the HNF4alpha binding site markedly reduced basal promoter activity and its repression by bile acids. Cotransfection with FTF strongly repressed CYP8B1 transcription. Interestingly, HNF4alpha could overcome the inhibitory effects of FTF and bile acids. We conclude that FTF and HNF4alpha not only play critical roles on CYP8B1 gene transcription, but also mediate bile acid feedback inhibition. This study reveals a novel mechanism by which bile acids inhibit rat CYP8B1 gene transcription by inducing FTF and inhibiting HNF4alpha expression. (C) 2002 Elsevier Science B.V. All rights reserved.
Identifier
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<a href="http://doi.org/10.1016/s1388-1981(02)00186-5" target="_blank" rel="noreferrer noopener">10.1016/s1388-1981(02)00186-5</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2002
activation
bile acid synthesise
Biochemistry & Molecular Biology
Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids
Biophysics
biosynthesis
Cell Biology
Chiang J Y L
cholesterol 7-alpha-hydroxylase gene
CYP7A1
cytochrome P450
Eggertsen G
expression
feedback-regulation
Gene Regulation
Journal Article
liver microsomal metabolism
Nuclear Receptor
promoter
Receptor
repression
Yang Y Z
Zhang M
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.jsbmb.2006.02.005" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.jsbmb.2006.02.005</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
42-51
Issue
1
Volume
100
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Title
A name given to the resource
Estrogen-mediated regulation of CYP7B1: A possible role for controlling DHEA levels in human tissues
Publisher
An entity responsible for making the resource available
Journal of Steroid Biochemistry and Molecular Biology
Date
A point or period of time associated with an event in the lifecycle of the resource
2006
2006-07
Subject
The topic of the resource
7-alpha-hydroxylase; alzheimers-disease; bile-acid biosynthesis; Biochemistry & Molecular Biology; CYP7B1; dehydroepiandrosterone 7-hydroxylase; DHEA; Endocrinology & Metabolism; estrogen; fetal development; gene-expression; human oxysterol 7-alpha-hydroxylase; prostate; rat; receptor-beta; sex hormone biosynthesis; transcriptional regulation; vascular dementia
Creator
An entity primarily responsible for making the resource
Tang W J; Eggertsen G; Chiang J Y L; Norlin M
Description
An account of the resource
The current study examines regulation of CYP7B1, a DHEA 7 alpha-hydroxylase, by sex hormones. Transfection with estrogen receptor alpha and treatment with 17 P-estradiol in human embryonic kidney 293 cells significantly increased CYP7B1 catalytic activity and mRNA, and stimulated a human CYP7B1 reporter gene. Transfection with estrogen receptor P showed similar but less significant effects. In the absence of receptors, 17 P-estradiol suppressed CYP7B1 activity, suggesting that estrogenic effects may be different in cells not expressing receptors. Quantitation of CYP7B1 mRNA in adult and fetal human tissues showed markedly higher CYP7B1 mRNA levels in fetal tissues compared with the corresponding adult ones, except in the liver. This indicates a tissue-specific, developmental regulation of CYP7B1 and suggests an important function for this enzyme in fetal life. DHEA secreted by fetal adrenals is an essential precursor for placental estrogen formation. Since CYP7B1 diverts DHEA from the sex hormone biosynthetic pathway, estrogen receptor-mediated up-regulatio of CYP7B1 should lead to less DHEA available for sex hormone synthesis and may help to maintain normal levels of estrogens and androgens in human tissues, especially during fetal development. Regulation by estrogens may also be of importance in other processes where CYP7B1 is involved, including cholesterol homeostasis, cellular proliferation, and CNS function. (c) 2006 Elsevier Ltd. All rights reserved.
Identifier
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<a href="http://doi.org/10.1016/j.jsbmb.2006.02.005" target="_blank" rel="noreferrer noopener">10.1016/j.jsbmb.2006.02.005</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article
2006
7-alpha-hydroxylase
alzheimers-disease
bile-acid biosynthesis
Biochemistry & Molecular Biology
Chiang J Y L
CYP7B1
dehydroepiandrosterone 7-hydroxylase
DHEA
Eggertsen G
Endocrinology & Metabolism
estrogen
fetal development
gene-expression
human oxysterol 7-alpha-hydroxylase
Journal Article
Journal of Steroid Biochemistry and Molecular Biology
Norlin M
Prostate
rat
receptor-beta
sex hormone biosynthesis
Tang W J
transcriptional regulation
vascular dementia