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Text
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URL Address
<a href="http://doi.org/10.1007/bf00944199" target="_blank" rel="noreferrer noopener">http://doi.org/10.1007/bf00944199</a>
Pages
21–26
Issue
1
Volume
139
Dublin Core
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Title
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Induction of ethanol dependence increases signal peptidase mRNA levels in rat brain.
Publisher
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Molecular and cellular biochemistry
Date
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1994
1994-10
Subject
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*Membrane Proteins; *Serine Endopeptidases; Alcoholism/*enzymology; Amino Acid; Amino Acid Sequence; Animals; Base Sequence; Blotting; Brain/*enzymology; Complementary; DNA; Endopeptidases/*biosynthesis/chemistry/genetics; Ethanol/*pharmacology; Male; Messenger/genetics/metabolism; Molecular Sequence Data; Northern; Nucleic Acid; Rats; RNA; Sequence Homology; Sprague-Dawley; Substance Withdrawal Syndrome/enzymology
Creator
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Signs S A; Jacquet R
Description
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Differential Northern blot hybridization was used as a screening tool to identify mRNAs that respond quantitatively to the induction of ethanol dependence. Adult male rats were treated with repeated, high doses of ethanol for 4 consecutive days. This regimen resulted in the development of tolerance and dependence upon ethanol. RNA isolated from the ethanol-dependent rat brains was used to construct a cDNA library. One cDNA was identified that hybridized to a mRNA which increased in rat brain during the ethanol treatment. Sequence analysis of the cDNA indicated that it recognized a mRNA in rat brain which was very similar to that which encodes the 18 kDa subunit of canine signal peptidase. The rat signal peptidase mRNA was observed to increase in brain nearly 2-fold within 48 h after the initiation of ethanol treatment. Ethanol did not significantly alter beta-actin mRNA levels during the treatment period. These results support the existence of an ethanol-responsive signal peptidase mRNA in rat brain.
Identifier
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<a href="http://doi.org/10.1007/bf00944199" target="_blank" rel="noreferrer noopener">10.1007/bf00944199</a>
Rights
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Membrane Proteins
*Serine Endopeptidases
1994
Alcoholism/*enzymology
Amino Acid
Amino Acid Sequence
Animals
Base Sequence
Blotting
Brain/*enzymology
Complementary
DNA
Endopeptidases/*biosynthesis/chemistry/genetics
Ethanol/*pharmacology
Jacquet R
Male
Messenger/genetics/metabolism
Molecular and cellular biochemistry
Molecular Sequence Data
Northern
Nucleic Acid
Rats
RNA
Sequence Homology
Signs S A
Sprague-Dawley
Substance Withdrawal Syndrome/enzymology