1
40
3
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1369/jhc.2009.953893" target="_blank" rel="noreferrer noopener">http://doi.org/10.1369/jhc.2009.953893</a>
Pages
923–931
Issue
10
Volume
57
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Advanced osteoarthritis in humans is associated with altered collagen VI expression and upregulation of ER-stress markers Grp78 and bag-1.
Publisher
An entity responsible for making the resource available
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Date
A point or period of time associated with an event in the lifecycle of the resource
2009
2009-10
Subject
The topic of the resource
Adult; Humans; Middle Aged; Osteoarthritis; Biomarkers/metabolism; Proteoglycans/biosynthesis; Down-Regulation; Up-Regulation; Cartilage; Antigens/biosynthesis; Bone Neoplasms/metabolism; Chondrosarcoma/metabolism; Collagen Type VI/*biosynthesis; DNA-Binding Proteins/*biosynthesis; Endoplasmic Reticulum/*metabolism; Heat-Shock Proteins/*biosynthesis; Osteosarcoma/metabolism; Transcription Factors/*biosynthesis; Articular/metabolism; Knee/*metabolism/physiopathology
Creator
An entity primarily responsible for making the resource
Nugent Ashleigh E; Speicher Danielle M; Gradisar Ian; McBurney Denise L; Baraga Anthony; Doane Kathleen J; Horton Walter E Jr
Description
An account of the resource
To test the hypothesis that a perturbation of endoplasmic reticulum (ER) function is involved in the pathogenesis of osteoarthritis (OA), articular cartilage was isolated from non-OA patients secondary to resection of osteo- or chondrosarcomas. Intra-joint samples of minimal and advanced osteoarthritic cartilage were isolated from patients undergoing total knee arthroplasty and scored for disease severity. Glucose-regulated protein-78 (grp78) and bcl-2-associated athanogene-1 (bag-1) were detected via immunofluorescence as markers of non-homeostatic ER function. Additionally, the expression of type VI collagen and its integrin receptor, NG2, was determined to examine cartilage matrix health and turnover. There was an upregulation of grp78 in advanced OA, and variable expression in minimal OA. Non-OA cartilage was consistently grp78 negative. The downstream regulator bag-1 was also upregulated in OA compared with normal cartilage. Collagen VI was mainly cell-associated in non-OA cartilage, with a more widespread distribution observed in OA cartilage along with increased intracellular staining intensity. The collagen VI integral membrane proteoglycan receptor NG2 was downregulated in advanced OA compared with its patient-matched minimally involved cartilage sample. These results suggest that chondrocytes exhibit ER stress during OA, in association with upregulation of a large secreted molecule, type VI collagen.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1369/jhc.2009.953893" target="_blank" rel="noreferrer noopener">10.1369/jhc.2009.953893</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2009
Adult
Antigens/biosynthesis
Articular/metabolism
Baraga Anthony
Biomarkers/metabolism
Bone Neoplasms/metabolism
Cartilage
Chondrosarcoma/metabolism
Collagen Type VI/*biosynthesis
Department of Anatomy & Neurobiology
DNA-Binding Proteins/*biosynthesis
Doane Kathleen J
Down-Regulation
Endoplasmic Reticulum/*metabolism
Gradisar Ian
Heat-Shock Proteins/*biosynthesis
Horton Walter E Jr
Humans
Knee/*metabolism/physiopathology
McBurney Denise L
Middle Aged
NEOMED College of Medicine
Nugent Ashleigh E
Osteoarthritis
Osteosarcoma/metabolism
Proteoglycans/biosynthesis
Speicher Danielle M
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Transcription Factors/*biosynthesis
Up-Regulation
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1074/jbc.M501069200" target="_blank" rel="noreferrer noopener">http://doi.org/10.1074/jbc.M501069200</a>
Pages
31156–31165
Issue
35
Volume
280
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Multiple signals induce endoplasmic reticulum stress in both primary and immortalized chondrocytes resulting in loss of differentiation, impaired cell growth, and apoptosis.
Publisher
An entity responsible for making the resource available
The Journal of biological chemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-09
Subject
The topic of the resource
Animals; Annexin A5/metabolism; Anti-Bacterial Agents/metabolism; Apoptosis/*physiology; Biomarkers; Caspase 12; Caspases/metabolism; CCAAT-Enhancer-Binding Proteins/metabolism; Cell Differentiation/*physiology; Cells; Chondrocytes/cytology/*physiology; Collagen Type II/metabolism; Cultured; DNA Fragmentation; Endoplasmic Reticulum/*metabolism; Extracellular Matrix/metabolism; Gene Expression Regulation; Glucose/metabolism; Proliferating Cell Nuclear Antigen/metabolism; Rats; Signal Transduction/*physiology; Thapsigargin/metabolism; Transcription Factor CHOP; Transcription Factors/metabolism; Tunicamycin/metabolism
Creator
An entity primarily responsible for making the resource
Yang Ling; Carlson Sara G; McBurney Denise; Horton Walter E Jr
Description
An account of the resource
The endoplasmic reticulum is the site of synthesis and folding of secretory proteins and is sensitive to changes in the internal and external environment of the cell. Both physiological and pathological conditions may perturb the function of the endoplasmic reticulum, resulting in endoplasmic reticulum stress. The chondrocyte is the only resident cell found in cartilage and is responsible for synthesis and turnover of the abundant extracellular matrix and may be sensitive to endoplasmic reticulum stress. Here we report that glucose withdrawal, tunicamycin, and thapsigargin induce up-regulation of GADD153 and caspase-12, two markers of endoplasmic reticulum stress, in both primary chondrocytes and a chondrocyte cell line. Other agents such as interleukin-1beta or tumor necrosis factor alpha induced a minimal or no induction of GADD153, respectively. The endoplasmic reticulum stress resulted in decreased chondrocyte growth based on cell counts, up-regulation of p21, and decreased PCNA expression. In addition, perturbation of endoplasmic reticulum function resulted in decreased accumulation of an Alcian Blue positive matrix by chondrocytes and decreased expression of type II collagen at the protein level. Further, quantitative real-time PCR was used to demonstrate a down-regulation of steady state mRNA levels coding for aggrecan, collagen II, and link protein in chondrocytes exposed to endoplasmic reticulum stress-inducing conditions. Ultimately, endoplasmic reticulum stress resulted in chondrocyte apoptosis, as evidenced by DNA fragmentation and annexin V staining. These findings have potentially important implications regarding consequences of endoplasmic reticulum stress in cartilage biology.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1074/jbc.M501069200" target="_blank" rel="noreferrer noopener">10.1074/jbc.M501069200</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2005
Animals
Annexin A5/metabolism
Anti-Bacterial Agents/metabolism
Apoptosis/*physiology
Biomarkers
Carlson Sara G
Caspase 12
Caspases/metabolism
CCAAT-Enhancer-Binding Proteins/metabolism
Cell Differentiation/*physiology
Cells
Chondrocytes/cytology/*physiology
Collagen Type II/metabolism
Cultured
Department of Anatomy & Neurobiology
DNA Fragmentation
Endoplasmic Reticulum/*metabolism
Extracellular Matrix/metabolism
Gene Expression Regulation
Glucose/metabolism
Horton Walter E Jr
McBurney Denise
NEOMED College of Medicine
Proliferating Cell Nuclear Antigen/metabolism
Rats
Signal Transduction/*physiology
Thapsigargin/metabolism
The Journal of biological chemistry
Transcription Factor CHOP
Transcription Factors/metabolism
Tunicamycin/metabolism
Yang Ling
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1002/jcb.23025" target="_blank" rel="noreferrer noopener">http://doi.org/10.1002/jcb.23025</a>
Pages
1118–1129
Issue
4
Volume
112
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
The presence of extracellular matrix alters the chondrocyte response to endoplasmic reticulum stress.
Publisher
An entity responsible for making the resource available
Journal of cellular biochemistry
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-04
Subject
The topic of the resource
*Stress; Animals; Apoptosis/drug effects; Articular/cytology; Blotting; Cartilage; Cattle; Cells; Chondrocytes/cytology/drug effects/*metabolism; Cultured; DNA-Binding Proteins/genetics/metabolism; Dose-Response Relationship; Drug; Endoplasmic Reticulum/*metabolism; Extracellular Matrix/*metabolism; Glucose/pharmacology; Heat-Shock Proteins/genetics/metabolism; Physiological; Proto-Oncogene Proteins c-bcl-2/genetics/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Thapsigargin/pharmacology; Time Factors; Transcription Factors/genetics/metabolism; Tunicamycin/pharmacology; Western
Creator
An entity primarily responsible for making the resource
Nugent Ashleigh E; McBurney Denise L; Horton Walter E Jr
Description
An account of the resource
The objective of this study was to test the hypothesis that extracellular matrix (ECM) would alter the endoplasmic reticulum (ER) stress response of chondrocytes. Chondrocytes were isolated from calf knees and maintained in monolayer culture or suspended in collagen I to form spot cultures (SCs). Our laboratory has shown that bovine chondrocytes form cartilage with properties similar to native cartilage after 2-4 weeks in SCs. Monolayer cultures treated with ER stressors glucose withdrawal (-Glu), tunicamycin (TN), or thapsigargin (TG) up-regulated Grp78 and Gadd153, demonstrating a complete ER stress response. SCs were grown at specific times from 1 day to 6 weeks before treatment with ER stressors. Additionally, SCs grown for 1, 2, or 6 weeks were treated with increasing concentrations of TN or TG. Western blotting of SCs for Grp78 indicated that increased ECM accumulation results in delayed expression; however, Grp78 mRNA is up-regulated in response to ER stressors even after 6 weeks in culture. SCs treated with ER stressors did not up-regulate Gadd153, suggesting that the cells experienced ER stress but would not undergo apoptosis. In fact, SCs undergo apoptosis upon ER stress treatment after 0-1 day of growth; however, after 4 days and to 6 weeks, apoptosis in treated samples was not different than controls. Pro-survival molecules Bcl-2 and Bag-1 were up-regulated upon ER stress in SCs. These results suggest that presence of ECM confers protection from ER stressors. Future studies involving chondrocyte physiology should focus on responses in conditions more closely mimicking the in vivo cartilage environment.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1002/jcb.23025" target="_blank" rel="noreferrer noopener">10.1002/jcb.23025</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Stress
2011
Animals
Apoptosis/drug effects
Articular/cytology
Blotting
Cartilage
Cattle
Cells
Chondrocytes/cytology/drug effects/*metabolism
Cultured
Department of Anatomy & Neurobiology
DNA-Binding Proteins/genetics/metabolism
Dose-Response Relationship
Drug
Endoplasmic Reticulum/*metabolism
Extracellular Matrix/*metabolism
Glucose/pharmacology
Heat-Shock Proteins/genetics/metabolism
Horton Walter E Jr
Journal of cellular biochemistry
McBurney Denise L
NEOMED College of Medicine
Nugent Ashleigh E
Physiological
Proto-Oncogene Proteins c-bcl-2/genetics/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Thapsigargin/pharmacology
Time Factors
Transcription Factors/genetics/metabolism
Tunicamycin/pharmacology
Western