Apical excrescences in the gallbladder epithelium of the female Syrian hamster in response to medroxyprogesterone.
Animals; Bile/metabolism; Cholelithiasis/*chemically induced/pathology; Cholesterol/metabolism; Cricetinae; Electron; Epithelium/ultrastructure; Female; Gallbladder/*drug effects/metabolism/ultrastructure; Medroxyprogesterone Acetate/*pharmacology/toxicity; Mesocricetus/*anatomy & histology; Microscopy; Mucus/metabolism; Ovariectomy; Scanning; Sex Factors
All the intact female Syrian hamsters treated with medroxyprogesterone (MP) for a one-month period, without dietary manipulation, display gallbladder surface epithelial changes, and intraluminal deposits. These changes include excrescences in various stages, bulging, and extrusion of material from the epithelial cells. The most striking scanning electron microscopic observations are the dramatic events, comparable to apocrine-like secretory events observed in another related study using oophorectomized hamsters. Since the hamster gallbladder does not possess mucous goblet cells, it appears that this phenomenon could be a response to the MP treatment, thus providing a larger amount of mucous product than usual with cellular material, in addition to the possible alteration in the quality of the bile following this treatment. As a result of MP treatment, intraluminal deposits were also confirmed by using light and transmission electron microscopy. In control hamsters these events were not observed, however, small blebs outlining surface epithelial cells are seen. The results in this report complement the previous studies using the male and oophorectomized Syrian hamster model subjected to similar experimental conditions.
Gilloteaux J; Karkare S; Kelly T R
The Anatomical record
1993
1993-07
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1002/ar.1092360308" target="_blank" rel="noreferrer noopener">10.1002/ar.1092360308</a>
Human gallbladder mucosa ultrastructure: evidence of intraepithelial nerve structures.
Electron/methods; Epithelial Cells; Epithelium/ultrastructure; Gallbladder/cytology/innervation/*ultrastructure; Humans; Mast Cells/ultrastructure; Microscopy; Mucous Membrane/cytology/ultrastructure; Nerve Endings/ultrastructure; Osmium Tetroxide; Staining and Labeling/methods; Zinc
The zinc iodide-osmium tetroxide (ZIO) fixative-staining method was used along with topographical ultrastructure to investigate cholecystectomized human gallbladders under light and electron microscopic techniques. This method delineated neuronal structures which may be involved in controlling the functions of the gallbladder epithelium. Three epithelial cell types were described in the surface epithelium: 1) Columnar clear cells; 2) dark, tuft osmiophilic cells; and 3) basal clear cells with electron-dense granules and showing intense ZIOphilic staining properties. While mucous granules were delineated in the first two cell types, the columnar epithelial clear cells are of uncertain function(s) and content but are probably absorptive cells. The small basal clear cells displaying intense ZIOphilia are associated with intraepithelial nerve endings. These nerve structures may have a sensory and/or motor function(s); they were detected throughout the gallbladder epithelial lining and mucosa.
Gilloteaux J; Pomerants B; Kelly T R
The American journal of anatomy
1989
1989-04
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1002/aja.1001840407" target="_blank" rel="noreferrer noopener">10.1002/aja.1001840407</a>