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URL Address
<a href="http://doi.org/10.1161/HYPERTENSIONAHA.107.098459" target="_blank" rel="noreferrer noopener">http://doi.org/10.1161/HYPERTENSIONAHA.107.098459</a>
Pages
704–711
Issue
3
Volume
51
Dublin Core
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Title
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Angiotensin II-induced extracellular signal-regulated kinase 1/2 activation is mediated by protein kinase Cdelta and intracellular calcium in adult rat cardiac fibroblasts.
Publisher
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Hypertension (Dallas, Tex. : 1979)
Date
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2008
2008-03
Subject
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Acetophenones/pharmacology; Angiotensin II/*physiology; Animals; Benzopyrans/pharmacology; Calcium/*metabolism; Cell Proliferation; Cells; Cultured; Enzyme Activation; ErbB Receptors/metabolism; Fibroblasts/*metabolism; Male; Mitogen-Activated Protein Kinase 1/*metabolism; Mitogen-Activated Protein Kinase 3/*metabolism; Myocardium/*cytology/metabolism; Phorbol Esters/pharmacology; Phosphorylation; Protein Kinase C-delta/genetics/*metabolism; Rats; Signal Transduction/physiology; Sprague-Dawley
Creator
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Olson Erik R; Shamhart Patricia E; Naugle Jennifer E; Meszaros J Gary
Description
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Angiotensin II (Ang II)-induced proliferation of cardiac fibroblasts is a major contributing factor to the pathogenesis of cardiac fibrosis. Ang II activates extracellular signal-regulated kinase (ERK) 1/2 to induce cardiac fibroblast proliferation, but the signaling pathways leading to ERK 1/2 activation have not been elucidated in these cells. The goal of the current study was to identify the intracellular mediators of Ang II-induced ERK 1/2 activation in adult rat cardiac fibroblasts. We determined that 100 nmol/L of Ang II-induced ERK 1/2 phosphorylation is inhibited by simultaneous chelation of cytosolic calcium and downregulation of protein kinase C (PKC) by phorbol ester or by the specific PKCdelta inhibitor rottlerin, as well as PKCdelta small interfering RNA, but not by inhibition of 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate, phorbol ester, rottlerin, or PKCdelta small interfering RNA alone. We also found that Ang II does not transactivate the epidermal growth factor receptor in adult cardiac fibroblasts, because pretreatment with 1 mumol/L of AG 1478 did not significantly inhibit [(3)H]-thymidine incorporation or ERK 1/2 activation. In addition, immunoprecipitation of the epidermal growth factor receptor demonstrated no significant Ang II-induced phosphorylation of tyrosine residues. Inhibition of phosphatidylinositide 3-kinase, PKCzeta, and src tyrosine kinase had no effect on Ang II-induced ERK 1/2 activation. Collectively, these data demonstrate that Ang II does not transactivate the epidermal growth factor receptor in adult rat cardiac fibroblasts to activate ERK 1/2, a common pathway described in vascular smooth muscle and other cell types, but rather occurs via activation of distinct parallel signaling pathways mechanistically controlled by intracellular Ca(2+) and PKCdelta.
Identifier
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<a href="http://doi.org/10.1161/HYPERTENSIONAHA.107.098459" target="_blank" rel="noreferrer noopener">10.1161/HYPERTENSIONAHA.107.098459</a>
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Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2008
Acetophenones/pharmacology
Angiotensin II/*physiology
Animals
Benzopyrans/pharmacology
Calcium/*metabolism
Cell Proliferation
Cells
Cultured
Department of Integrative Medical Sciences
Enzyme Activation
ErbB Receptors/metabolism
Fibroblasts/*metabolism
Hypertension (Dallas, Tex. : 1979)
Male
Meszaros J Gary
Mitogen-Activated Protein Kinase 1/*metabolism
Mitogen-Activated Protein Kinase 3/*metabolism
Myocardium/*cytology/metabolism
Naugle Jennifer E
NEOMED College of Medicine
Olson Erik R
Phorbol Esters/pharmacology
Phosphorylation
Protein Kinase C-delta/genetics/*metabolism
Rats
Shamhart Patricia E
Signal Transduction/physiology
Sprague-Dawley