Epithelial surface changes and induction of gallstones in the male Syrian hamster gallbladder as a result of a two-month sex steroid treatment.
Male; Animals; Body Weight/drug effects; Reference Values; Cricetinae; Mesocricetus; Estradiol/*toxicity; Medroxyprogesterone/*toxicity; Cell Membrane/pathology/ultrastructure; Cholelithiasis/chemically induced/*pathology; Cytoplasmic Granules/ultrastructure; Epithelium/pathology/ultrastructure; Gallbladder/drug effects/*pathology/ultrastructure; Vacuoles/ultrastructure; Microscopy; Electron; Scanning
Transmission (TEM) and scanning (SEM) electron microscopic observations were correlated to characterize morphologic changes induced in the gallbladder of male Syrian hamsters following a two-month estradiol (E) and estradiol + medroxyprogesterone (E + MP) treatment. Compared to control (C), E-treated surface epithelial cells show pleomorphism, cytoplasmic vacuolizations, apical granules, excrescences and decapitations, and small gallstone-like deposits. Following both E + MP treatment, a large accumulation of apical granules containing acidic mucoid products, abundant intraluminal deposits and numerous fields of observation suggest that cell debris and mucous condensation could participate in the formation of the large intraluminal gallstone-like deposits detected as a result of this treatment. In control gallbladders these events were never observed. MP added to E also increases liver and gallbladder weight as well as blood lipid levels. These findings complement and confirm other previous data obtained following short steroid treatment in male, ovariectomized and intact female hamsters. In addition, these results support our hypothesis that gallstone nucleation and growth originate from multiple factors, hormonal disturbance, modulation of liver lipid metabolism, production of cell debris and mucus, can be responsible for the initial gallstone nucleation.
Gilloteaux J; Kosek E; Kelly T R
Journal of submicroscopic cytology and pathology
1993
1993-10
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Gallstones induced by sex steroids in the female Syrian hamster: duration effects.
Female; Time Factors; Animals; Body Weight; Lipids/blood; Organ Size; Cricetinae; Mesocricetus; Epithelium/drug effects/ultrastructure; Cholelithiasis/*chemically induced; Estradiol/*toxicity; Gallbladder/*drug effects/pathology; Medroxyprogesterone/*toxicity; Microscopy; Electron
Scanning (SEM), and transmission (TEM) electron microscopy were used and correlated to morphologically characterize changes induced in the gallbladder epithelium of female Syrian hamster in response to treatments with estradiol (E) alone, and estradiol with medroxyprogesterone (E + MP). Compared with control (C), the E- and E + MP-treated groups demonstrated alterations in the serum lipid profile as well as significantly decreased body weights. The liver with gallbladder weights, as well as the uterus weights, were significantly increased. Two-month E and E + MP treatment groups exhibited increased number of anionically charged apical granules, and luminal mucoid elements. Contrastingly, the three-month treatment groups demonstrated larger and more gallstone-like deposits as compared to the C and two-month E and E + MP groups. This report presents a comprehensive overview of our previous and current data, including that of other investigators in relation to the various factors and parameters involved in the cholelithiatic process.
Karkare S; Gilloteaux J
Journal of submicroscopic cytology and pathology
1995
1995-01
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Morphological aspects of female Syrian hamster gallbladder induced by one-month sex steroid treatment.
Female; Animals; Body Weight; Lipids/blood; Organ Size; Cricetinae; Mesocricetus; Epithelium/drug effects/ultrastructure; Estradiol/*toxicity; Medroxyprogesterone/*toxicity; Gallbladder/*drug effects/metabolism/ultrastructure; Microscopy; Electron; Scanning
Light (LM), transmission (TEM), and scanning (SEM) electron microscopy were used to characterize morphological changes induced in the gallbladder epithelium of female Syrian hamsters in response to one-month estradiol alone (E) and estradiol with medroxyprogesterone (E + MP) treatments. TEM data were correlated with the SEM observations. Compared with control (C), E- and E + MP-treated hamsters showed significant decreases in body weight, while the liver and gallbladder, and uterus weights increased. Moreover, E treatment induced some subcellular changes (microvilli, nucleus, mitochondria, RER, glycogen, abundant apical granules). The E + MP treatment appeared to exacerbate these similar changes and, in addition, induced apical excrescences and cell shedding. Both E and E + MP gallbladders showed luminal micelles, cellular debris and crystalliths associated with mucus. Simultaneously, an increased acidification of the mucoid content of the apical granules was noticed.
Karkare S; Kelly T R; Gilloteaux J
Journal of submicroscopic cytology and pathology
1995
1995-01
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).