1
40
3
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.3109/01902148.2010.514024" target="_blank" rel="noreferrer noopener">http://doi.org/10.3109/01902148.2010.514024</a>
Pages
44–56
Issue
1
Volume
37
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Lung fluid absorption is induced in preterm guinea pigs ventilated with low tidal volumes.
Publisher
An entity responsible for making the resource available
Experimental lung research
Date
A point or period of time associated with an event in the lifecycle of the resource
2011
2011-02
Subject
The topic of the resource
Time Factors; Animals; Gestational Age; Guinea Pigs; *Premature Birth; Phosphorylation; Enzyme Activation; Permeability; Extracellular Signal-Regulated MAP Kinases/metabolism; Intubation; Epithelial Sodium Channels/metabolism; Absorption; Sodium-Potassium-Exchanging ATPase/metabolism; Fetal Organ Maturity; Epinephrine/blood; Extravascular Lung Water/*metabolism; *Tidal Volume; Albumins/administration & dosage/*metabolism; Hydrocortisone/blood; Lung/embryology/*metabolism/physiopathology; MAP Kinase Kinase Kinases/metabolism; Ventilator-Induced Lung Injury/metabolism/physiopathology/*prevention & control; Newborn; Intratracheal; Artificial/adverse effects/*methods; Respiration
Creator
An entity primarily responsible for making the resource
Koshy Shyny; Beard LaMonta L; Kuzenko Stephanie R; Li Tianbo; Folkesson Hans G
Description
An account of the resource
The objective of this study was to determine if low tidal volume (V(t)) ventilation was beneficial when ventilating preterm fetuses. The authors ventilated preterm guinea pig fetuses at gestation day (GD) 67, 3 days before birth, newborn, and 10-day-old (PD10) guinea pigs with low V(t) (6 mL/kg body weight [bw]) and compared them to age-matched fetuses/animals ventilated with higher potentially injurious V(t) (12 mL/kg bw). Lung fluid absorption was measured after intratracheal instillation of 5% albumin in 0.9% NaCl. Low V(t) ventilation stimulated lung fluid absorption when compared to higher V(t) in all groups. The increased lung fluid absorption in low V(t)-ventilated fetuses was associated with increased alpha epithelial Na channel (alphaEnaC) mRNA. However, alphaENaC and betaENaC protein was unchanged over the 1-hour study. Because stretch induces mitogen-activated protein (MAP) kinase expression and MAP kinases may affect lung fluid absorption, the authors investigated if MAP kinase (MAPK) expression was affected by V(t). Extracellular signal-regulated kinase (ERK) and MAPK/ERK kinase (MEK) were phosphorylated in the higher V(t)-ventilated guinea pig fetuses. This suggested that a reduced activation of MAP kinases might explain the increased lung fluid absorption in the low V(t)-ventilated fetuses. Thus these data suggest that low V(t) ventilation increases fetal lung fluid absorption and thus may be preferential to use clinically.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.3109/01902148.2010.514024" target="_blank" rel="noreferrer noopener">10.3109/01902148.2010.514024</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Premature Birth
*Tidal Volume
2011
Absorption
Albumins/administration & dosage/*metabolism
Animals
Artificial/adverse effects/*methods
Beard LaMonta L
Enzyme Activation
Epinephrine/blood
Epithelial Sodium Channels/metabolism
Experimental lung research
Extracellular Signal-Regulated MAP Kinases/metabolism
Extravascular Lung Water/*metabolism
Fetal Organ Maturity
Folkesson Hans G
Gestational Age
Guinea Pigs
Hydrocortisone/blood
Intratracheal
Intubation
Koshy Shyny
Kuzenko Stephanie R
Li Tianbo
Lung/embryology/*metabolism/physiopathology
MAP Kinase Kinase Kinases/metabolism
Newborn
Permeability
Phosphorylation
Respiration
Sodium-Potassium-Exchanging ATPase/metabolism
Time Factors
Ventilator-Induced Lung Injury/metabolism/physiopathology/*prevention & control
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1371/journal.pone.0115325" target="_blank" rel="noreferrer noopener">http://doi.org/10.1371/journal.pone.0115325</a>
Pages
e0115325–e0115325
Issue
2
Volume
10
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Integrin mediated adhesion of osteoblasts to connective tissue growth factor (CTGF/CCN2) induces cytoskeleton reorganization and cell differentiation.
Publisher
An entity responsible for making the resource available
PloS one
Date
A point or period of time associated with an event in the lifecycle of the resource
2015
2015
Subject
The topic of the resource
Animals; Mice; Signal Transduction; Cell Line; Cell Adhesion; *Cell Differentiation; Connective Tissue Growth Factor/chemistry/*metabolism; Core Binding Factor Alpha 1 Subunit/metabolism; Cytoskeleton/*metabolism; Extracellular Signal-Regulated MAP Kinases/metabolism; Focal Adhesion Protein-Tyrosine Kinases/metabolism; Integrins/*metabolism; Osteoblasts/*cytology/*metabolism; rac GTP-Binding Proteins/metabolism; Transcriptional Activation; Receptors; Vitronectin/metabolism
Creator
An entity primarily responsible for making the resource
Hendesi Honey; Barbe Mary F; Safadi Fayez F; Monroy M Alexandra; Popoff Steven N
Description
An account of the resource
Pre-osteoblast adhesion and interaction with extracellular matrix (ECM) proteins through integrin receptors result in activation of signaling pathways regulating osteoblast differentiation. Connective tissue growth factor (CTGF/CCN2) is a matricellular protein secreted into the ECM. Prior studies in various cell types have shown that cell adhesion to CTGF via integrin receptors results in activation of specific signaling pathways that regulate cell functions, such as differentiation and cytoskeletal reorganization. To date, there are no studies that have examined whether CTGF can serve as an adhesive substrate for osteoblasts. In this study, we used the MC3T3-E1 cell line to demonstrate that CTGF serves as an adhesive matrix for osteoblasts. Anti-integrin blocking experiments and co-immunoprecipitation assays demonstrated that the integrin alphavbeta1 plays a key role in osteoblast adhesion to a CTGF matrix. Immunofluorescence staining of osteoblasts cultured on a CTGF matrix confirmed actin cytoskeletal reorganization, enhanced spreading, formation of focal adhesions, and activation of Rac1. Alkaline phosphatase (ALP) staining and activity assays, as well as Alizarin red staining demonstrated that osteoblast attachment to CTGF matrix enhanced maturation, bone nodule formation and matrix mineralization. To investigate whether the effect of CTGF on osteoblast differentiation involves integrin-mediated activation of specific signaling pathways, we performed Western blot, chromatin immunoprecipitation (ChIP) and qPCR assays. Osteoblasts cultured on a CTGF matrix showed increased total and phosphorylated (activated) forms of focal adhesion kinase (FAK) and extracellular signal-regulated kinase (ERK). Inhibition of ERK blocked osteogenic differentiation in cells cultured on a CTGF matrix. There was an increase in runt-related transcription factor 2 (Runx2) binding to the osteocalcin gene promoter, and in the expression of osteogenic markers regulated by Runx2. Collectively, the results of this study are the first to demonstrate CTGF serves as a suitable matrix protein, enhancing osteoblast adhesion (via alphavbeta1 integrin) and promoting cell spreading via cytoskeletal reorganization and Rac1 activation. Furthermore, integrin-mediated activation of ERK signaling resulted in increased osteoblast differentiation accompanied by an increase in Runx2 binding to the osteocalcin promoter and in the expression of osteogenic markers.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1371/journal.pone.0115325" target="_blank" rel="noreferrer noopener">10.1371/journal.pone.0115325</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
*Cell Differentiation
2015
Animals
Barbe Mary F
Cell Adhesion
Cell Line
Connective Tissue Growth Factor/chemistry/*metabolism
Core Binding Factor Alpha 1 Subunit/metabolism
Cytoskeleton/*metabolism
Department of Anatomy & Neurobiology
Extracellular Signal-Regulated MAP Kinases/metabolism
Focal Adhesion Protein-Tyrosine Kinases/metabolism
Hendesi Honey
Integrins/*metabolism
Mice
Monroy M Alexandra
NEOMED College of Medicine
Osteoblasts/*cytology/*metabolism
PloS one
Popoff Steven N
rac GTP-Binding Proteins/metabolism
Receptors
Safadi Fayez F
Signal Transduction
Transcriptional Activation
Vitronectin/metabolism
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1186/1465-9921-8-27" target="_blank" rel="noreferrer noopener">http://doi.org/10.1186/1465-9921-8-27</a>
Pages
27–27
Volume
8
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Stimulation of MAP kinase pathways after maternal IL-1beta exposure induces fetal lung fluid absorption in guinea pigs.
Publisher
An entity responsible for making the resource available
Respiratory research
Date
A point or period of time associated with an event in the lifecycle of the resource
2007
2007-03
Subject
The topic of the resource
Absorption/drug effects; Animal/*metabolism; Animals; Body Fluids/*metabolism; Extracellular Signal-Regulated MAP Kinases/metabolism; Female; Fetus/metabolism; Gestational Age; Guinea Pigs; Hydrocortisone/*metabolism; Injections; Interleukin-1beta/administration & dosage/*pharmacology; Lung/*embryology; Mitogen-Activated Protein Kinase Kinases/metabolism; Mitogen-Activated Protein Kinases/*metabolism; Pregnancy; Rats; Recombinant Proteins/administration & dosage/pharmacology; Subcutaneous
Creator
An entity primarily responsible for making the resource
Bhattacharjee Reshma; Li Tianbo; Koshy Shyny; Beard LaMonta L; Sharma Kapil; Carter Ethan P; Garat Chrystelle; Folkesson Hans G
Description
An account of the resource
BACKGROUND: We tested the hypothesis that maternal interleukin-1beta (IL-1beta) pretreatment and induction of fetal cortisol synthesis activates MAP kinases and thereby affects lung fluid absorption in preterm guinea pigs. METHODS: IL-1beta was administered subcutaneously daily to timed-pregnant guinea pigs for three days. Fetuses were obtained by abdominal hysterotomy and instilled with isosmolar 5% albumin into the lungs and lung fluid movement was measured over 1 h by mass balance. MAP kinase expression was measured by western blot. RESULTS: Lung fluid absorption was induced at 61 days (D) gestation and stimulated at 68D gestation by IL-1beta. Maternal IL-1beta pretreatment upregulated ERK and upstream MEK expression at both 61 and 68D gestation, albeit being much more pronounced at 61D gestation. U0126 instillation completely blocked IL-1beta-induced lung fluid absorption as well as IL-1beta-induced/stimulated ERK expression. Cortisol synthesis inhibition by metyrapone attenuated ERK expression and lung fluid absorption in IL-1beta-pretreated fetal lungs. JNK expression after maternal
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1186/1465-9921-8-27" target="_blank" rel="noreferrer noopener">10.1186/1465-9921-8-27</a>
Rights
Information about rights held in and over the resource
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
2007
Absorption/drug effects
Animal/*metabolism
Animals
Beard LaMonta L
Bhattacharjee Reshma
Body Fluids/*metabolism
Carter Ethan P
Extracellular Signal-Regulated MAP Kinases/metabolism
Female
Fetus/metabolism
Folkesson Hans G
Garat Chrystelle
Gestational Age
Guinea Pigs
Hydrocortisone/*metabolism
Injections
Interleukin-1beta/administration & dosage/*pharmacology
Koshy Shyny
Li Tianbo
Lung/*embryology
Mitogen-Activated Protein Kinase Kinases/metabolism
Mitogen-Activated Protein Kinases/*metabolism
Pregnancy
Rats
Recombinant Proteins/administration & dosage/pharmacology
Respiratory research
Sharma Kapil
Subcutaneous