1 40 1 Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. URL Address <a href="http://doi.org/10.1006/excr.1998.4051" target="_blank" rel="noreferrer noopener">http://doi.org/10.1006/excr.1998.4051</a> Pages 230–241 Issue 1 Volume 241 Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Type VI collagen increases cell survival and prevents anti-beta 1 integrin-mediated apoptosis. Publisher An entity responsible for making the resource available Experimental cell research Date A point or period of time associated with an event in the lifecycle of the resource 1998 1998-05 Subject The topic of the resource Animals; Antibodies; Apoptosis/*drug effects/physiology; Cell Nucleus/drug effects/metabolism; Cell Survival/drug effects/physiology; Cells; Chick Embryo; Collagen/*pharmacology; Cornea/drug effects/embryology/ultrastructure; Culture Media/chemistry/pharmacology; Cultured; DNA Fragmentation/drug effects; Extracellular Matrix/physiology; Eye/drug effects/embryology/ultrastructure; Fibroblasts/drug effects/metabolism/ultrastructure; Genetic Techniques; Integrins/immunology; Monoclonal/*drug effects/pharmacology Creator An entity primarily responsible for making the resource Howell S J; Doane K J Description An account of the resource Cell-matrix interactions are important in the development of the avian cornea. Type VI collagen is present within the periocular mesenchyme prior to the migration of cells into the corneal stroma and is abundant in the mature stroma. Whether the interaction of cells with type VI collagen is essential for cellular survival in the cornea is not known. In the present study, we examined the interaction of corneal cells with type VI collagen in vitro to determine if it can increase cell proliferation and decrease apoptosis. In vivo analysis demonstrated that apoptosis occurs in the periocular region during early stages of avian corneal development, but in fully mature corneas apoptosis only occurs in the corneal epithelium and not in the stroma. In vitro analysis examined the importance of beta 1 integrin interactions with type VI collagen in mature corneal fibroblasts and the precursor cells. Using an anti-beta 1 integrin blocking antibody, CSAT, integrin/matrix interactions were disrupted. Results indicated that viability of both corneal fibroblasts and periocular mesenchyme cells was greater on type VI collagen than on type I collagen or BSA-blocked glass. In addition, less apoptosis was observed for both cell types on type VI collagen when beta 1 integrin–matrix interactions were disrupted. These data indicated that these cells require intact beta 1 interactions with type I collagen and with BSA-coated glass controls to remain viable. Thus, type VI collagen may play a role in the rescue of corneal cells from anti-beta 1 integrin-induced apoptosis by increasing cell survival, probably via a non-beta 1 integrin-dependent mechanism. Identifier An unambiguous reference to the resource within a given context <a href="http://doi.org/10.1006/excr.1998.4051" target="_blank" rel="noreferrer noopener">10.1006/excr.1998.4051</a> Rights Information about rights held in and over the resource Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s). 1998 Animals Antibodies Apoptosis/*drug effects/physiology Cell Nucleus/drug effects/metabolism Cell Survival/drug effects/physiology Cells Chick Embryo Collagen/*pharmacology Cornea/drug effects/embryology/ultrastructure Culture Media/chemistry/pharmacology Cultured DNA Fragmentation/drug effects Doane K J Experimental cell research Extracellular Matrix/physiology Eye/drug effects/embryology/ultrastructure Fibroblasts/drug effects/metabolism/ultrastructure Genetic Techniques Howell S J Integrins/immunology Monoclonal/*drug effects/pharmacology