1
40
2
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1016/j.aca.2013.03.053" target="_blank" rel="noreferrer noopener">http://doi.org/10.1016/j.aca.2013.03.053</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
41-49
Volume
779
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Differences In Infrared Spectroscopic Data Of Connective Tissues In Transflectance And Transmittance Modes
Publisher
An entity responsible for making the resource available
Analytica Chimica Acta
Date
A point or period of time associated with an event in the lifecycle of the resource
2013
2013-05
Subject
The topic of the resource
BaF2 window; bone; cartilage; Chemistry; collagen orientation; consequences; Fourier transform infrared imaging spectroscopy; Low-e; proteoglycan; scattering; slide; Transflectance; Transmittance; variance
Creator
An entity primarily responsible for making the resource
Hanifi A; McGoverin C; Ou Y T; Safadi F; Spencer R G; Pleshko N
Description
An account of the resource
Fourier transform infrared imaging spectroscopy (FT-IRIS) has been used extensively to characterize the composition and orientation of macromolecules in thin tissue sections. Earlier and current studies of normal and polarized FT-IRIS data have primarily used tissues sectioned onto infrared transmissive substrates, such as salt windows. Recently, the use of low-emissivity ("low-e") substrates has become of great interest because of their low cost and favorable infrared optical properties. However, data are collected in transflectance mode when using low-e slides and in transmittance mode using salt windows. In the current study we investigated the comparability of these two modes for assessment of the composition of connective tissues. FT-IRIS data were obtained in transflectance and transmittance modes from serial sections of cartilage, bone and tendon, and from a standard polymer, polymethylmethacrylate. Both non-polarized and polarized FTIR data differed in absorbance, and in some cases peak position, between transflectance and transmittance modes. However, the FT-IRIS analysis of the collagen fibril orientation in cartilage resulted in the expected zonal arrangement of fibrils in both transmittance and transflectance. We conclude that numerical comparison of FT-IRIS-derived parameters of tissue composition should account for substrate type and data collection mode, while analysis of overall tissue architecture may be more invariant between modes. (c) 2013 Elsevier B.V. All rights reserved.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1016/j.aca.2013.03.053" target="_blank" rel="noreferrer noopener">10.1016/j.aca.2013.03.053</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2013
Analytica Chimica Acta
BaF2 window
Bone
Cartilage
Chemistry
collagen orientation
consequences
Fourier transform infrared imaging spectroscopy
Hanifi A
Journal Article or Conference Abstract Publication
Low-e
McGoverin C
Ou Y T
Pleshko N
proteoglycan
Safadi F
Scattering
slide
Spencer R G
Transflectance
Transmittance
variance
-
Text
A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text.
URL Address
<a href="http://doi.org/10.1117/1.1922329" target="_blank" rel="noreferrer noopener">http://doi.org/10.1117/1.1922329</a>
Rights
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
Pages
6-6
Issue
3
Volume
10
Search for Full-text
Locate full-text within NEOMED Library's e-journal collections
<p>Users with a NEOMED Library login can search for full-text journal articles at the following url: <a href="https://libraryguides.neomed.edu/home">https://libraryguides.neomed.edu/home</a></p>
Dublin Core
The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/.
Title
A name given to the resource
Fourier Transform Infrared Imaging Spectroscopic Analysis Of Tissue Engineered Cartilage: Histologic And Biochemical Correlations
Publisher
An entity responsible for making the resource available
Journal of Biomedical Optics
Date
A point or period of time associated with an event in the lifecycle of the resource
2005
2005-05
Subject
The topic of the resource
Biochemistry & Molecular Biology; bioreactor; chondrocytes; collagen; Fourier transform infrared imaging spectroscopy; hollow fiber bioreactor; hollow-fiber; human articular-cartilage; magnetic-resonance microscopy; matrix; Medical Imaging; model; mri techniques; Nuclear Medicine &; Optics; osteoarthritis; proteoglycans; Radiology; tissue engineered cartilage; tissue engineering
Creator
An entity primarily responsible for making the resource
Kim M; Bi X H; Horton W E; Spencer R G; Camacho N P
Description
An account of the resource
The composition of cartilage is predictive of its in vivo performance. Therefore, the ability to assess its primary macromolecular components, proteoglycan (PG) and collagen, is of great importance. In the current study, we hypothesized that PG content and distribution in tissue engineered cartilage could be determined using Fourier-transform infrared imaging spectroscopy (FT-IRIS). The cartilage was grown from chondrocytes within a hollow fiber bioreactor (HFBR) system previously used extensively to study cartilage development. FT-IRIS analysis showed a gradient of PG content, with the highest content in the center near the nutritive fibers and the lowest near the interior surface of the HFBR. Further, we found significantly greater PG content in the region near culture medium inflow (45.0%) as compared to the outflow region (24.7%) (p < 0.001). This difference paralleled the biochemically determined glycosaminoglycan difference of 42.6% versus 27.8%. In addition, FT-IRIS-determined PG content at specific positions within the tissue sections correlated with histologically determined PG content (R=50.73, p=50.007). In summary, FT-IRIS determination of PG correlates with histological determination of PG and yields quantitatively similar results to biochemical determination of glycosaminoglycan in developing cartilage. (c) 2005 Society of Photo-Optical Instrumentation Engineers.
Identifier
An unambiguous reference to the resource within a given context
<a href="http://doi.org/10.1117/1.1922329" target="_blank" rel="noreferrer noopener">10.1117/1.1922329</a>
Format
The file format, physical medium, or dimensions of the resource
Journal Article or Conference Abstract Publication
2005
Bi X H
Biochemistry & Molecular Biology
bioreactor
Camacho N P
Chondrocytes
Collagen
Fourier transform infrared imaging spectroscopy
hollow fiber bioreactor
hollow-fiber
Horton W E
human articular-cartilage
Journal Article or Conference Abstract Publication
Journal of Biomedical Optics
Kim M
magnetic-resonance microscopy
matrix
Medical Imaging
model
mri techniques
Nuclear Medicine &
optics
Osteoarthritis
proteoglycans
Radiology
Spencer R G
tissue engineered cartilage
Tissue Engineering