Androgen-receptor defect abolishes sex differences in nitric oxide and reactivity to vasopressin in rat aorta
aorta; arginine vasopressin; binding; brain; dependent gender differences; dimorphism; Endothelium; endothelium-derived relaxing factor; estrogen; gonadal steroid hormones; hormones; l-arginine; muscle; Physiology; Sport Sciences; testicular feminized rat; testicular feminized rat; vasoconstriction
Contractions of rat thoracic aorta to vasopressin WP) are threefold higher in females (F) than in males (M), primarily because nitric oxide (NO) attenuation of contraction is greater in M. To determine the role of the androgen receptor (AR) in this mechanism, vascular reactivity to VP was examined in thoracic aorta of the testicular-feminized male (Tfm) rat, which has an X-linked, recessive defect in AR function in affected M. Maximal contraction of normal aortas to VP was fourfold higher in F (4,128 +/- 291 mg/mg ring wt) than in M (971 +/- 133 mg); maximal response of Tfm (3,967 +/- 253 mg) was similar to that of normal F. N-G-nitro-L-arginine methyl ester increased maximal response to VP threefold in M but had no effect in F or Tfm. In contrast, maximal contraction of normal aortas to phenylephrine was 43% higher in M (4,011 +/- 179 mg) than in F (2,809 +/- 78 mg); maximal response of Tfm (2,716 +/- 126 mg) was similar to that of normal F. N-G-nitro-L-arginine methyl ester increased maximal response to phenylephrine by >50% in F and Tfm but had no effect in M. Maximal contractile response to 80 mM KCl did not differ among M, F, or Tfm. Thus androgens and normal vascular AR function are important in the greater NO-mediated attenuation of reactivity to VP in M than in F rat aorta, which may involve specific modulation of endothelial VP signal transduction pathways and NO release by androgens. These data also establish the importance of the Tfm rat as a model to study the effects of androgens on cardiovascular function.
Stallone J N; Salisbury R L; Fulton C T
Journal of Applied Physiology
2001
2001-12
Journal Article
<a href="http://doi.org/10.1152/jappl.2001.91.6.2602" target="_blank" rel="noreferrer noopener">10.1152/jappl.2001.91.6.2602</a>
Constrictor Prostanoids Contribute To Sexual Dimorphism In Vasopressin (vp)-induced Contraction Of Rat Aorta
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Fulton C T; Stallone J N
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Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Silfani T N; Fulton C T; Wildroudt M L; Stallone J N; Freeman E J
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2000
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n/a
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Cardiovascular System & Cardiology
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Circulation
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Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Fulton C T; Stallone J N
Faseb Journal
1999
1999-03
Journal Article or Conference Abstract Publication
n/a
Tamoxifen (tmx) Mimics The Vascular Effects Of Estrogen (e-2) By Upregulating Constrictor Prostanoid Function By An E-2 Receptor-dependent Mechanism
Biochemistry & Molecular Biology; Cell Biology; Life Sciences & Biomedicine - Other; Topics
Fulton C T; Stallone J N; Freeman E J; Li M
Faseb Journal
2000
2000-03
Journal Article or Conference Abstract Publication
n/a