Pentobarbital differentially inhibits N-methyl-D-aspartate and kainate-stimulated [3H]noradrenaline overflow in rat cortical slices.
*Excitatory Amino Acid Agonists; Adrenergic alpha-Agonists/metabolism; Animals; GABA Modulators/*pharmacology; Kainic Acid/*antagonists & inhibitors; Male; N-Methylaspartate/*antagonists & inhibitors; Norepinephrine/metabolism; Pentobarbital/*pharmacology; Prefrontal Cortex/*drug effects; Rats; Sprague-Dawley
1. This study examined the ability of pentobarbital to inhibit NMDA and kainate-stimulated [3H]noradrenaline ([3H]NA) overflow in rat brain cortical slices. 2. Pentobarbital inhibited NMDA-evoked [3H]NA overflow at 100 microM and greater and inhibited kainate-evoked [3H]NA overflow at 10 microM and greater. 3. The ability of pentobarbital to inhibit concentration-response curves for NMDA and kainate-evoked overflow of [3H]NA were also examined. Pentobarbital (300 microM) caused a 20% reduction in NMDA and a 50% reduction in kainate-induced maximal responses.
Brown L M
General pharmacology
1995
1995-11
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/0306-3623(95)00057-7" target="_blank" rel="noreferrer noopener">10.1016/0306-3623(95)00057-7</a>
Phenobarbital pre-treatment prevents kainic acid-induced impairments in acquisition learning.
Animal/drug effects; Animals; Behavior; Cues; Excitatory Amino Acid Agonists/*toxicity; GABA Modulators/*pharmacology; Kainic Acid/*antagonists & inhibitors/*toxicity; Learning/drug effects; Long-Evans; Male; Maze Learning/*drug effects; Memory/drug effects; Phenobarbital/*pharmacology; Rats; Seizures/chemically induced/prevention & control
This study examined the protective effect of phenobarbital on kainic acid-induced deficits in acquisition learning. A single kainic acid injection (9 mg/kg i.p.) was administered five days prior to testing using the Morris water maze test. Kainic acid produced deficits in the acquisition of spatial information observed as an increase in latency to a hidden escape platform. Daily phenobarbital treatment (20 mg/kg i.p.) initiated 45 minutes prior to the kainic acid injection blocked the kainic acid-induced deficits in acquisition learning. When daily phenobarbital treatment was initiated 2-3 hours after kainic acid seizure development it did not block the kainic acid induced-deficits in water maze performance. Daily administration of phenobarbital alone at the moderate concentration used in this study did not cause alterations in behavioral performance in the Morris water maze. These studies indicate that phenobarbital pre-treatment results in a behavioral neuroprotection against kainic acid-induced neurotoxicity.
Brown-Croyts L M; Caton P W; Radecki D T; McPherson S L
Life sciences
2000
2000-06
Article information provided for research and reference use only. All rights are retained by the journal listed under publisher and/or the creator(s).
<a href="http://doi.org/10.1016/s0024-3205(00)00658-5" target="_blank" rel="noreferrer noopener">10.1016/s0024-3205(00)00658-5</a>